• Title/Summary/Keyword: root culture

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Improved Anthocyanin Production in Hairy Root Culture of Daucus carota by Fungal Elicitor (당근의 모상근 배양에서 Fungal Elicitor에 의한 Anthocyanin 생산의 향상)

  • Kim, Chang-Heon;Lee, Seong-Woo;Whang, Baik;Chung, In-Sik
    • Microbiology and Biotechnology Letters
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    • v.22 no.4
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    • pp.395-400
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    • 1994
  • In order to improve anthocyanin production, effects of fungal elicitation in hairy root culture were investigated. fungal elicitor prepared from Fusarium moniliforme was the best in enhancement of anthocyanin production among the eight fungal elicitors tested. The optimum treat-ment time and concentration of treated elicitor for anthocyanin production were 12 hours and 3.28 mg carbohydrates per liter medium. Also, fungal elicitor was treated to hairy root culture in flat-bottomed fluidized-bed bioreactor. The anthocyanin production of elicited culture was enhanced 227% than non-treated.

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Optimization of Submerged Culture Conditions for the Production of Ginseng Root Using Response Surface Method (반응표면분석법을 이용한 인삼 Root 액체배양조건의 최적화)

  • 오훈일;장은정;이시경;박동기
    • Journal of Ginseng Research
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    • v.24 no.2
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    • pp.58-63
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    • 2000
  • To develop the production of ginseng root using plant tissue culture technology, submerged culture conditions were optimized by means of the fractional factorial design with 4 factors and 3 levels by a RSM computer program. The ginseng (Panax ginseng C. A. Meyer) roots induced by plant growth regulators were cultured on SH medium and the effects of various pH of medium, sucrose concentration, nitrogen concentration and phosphate concentration on fresh weight of the ginseng root were investigated. The fresh weight of ginseng root increased with a decrease in nitrogen concentration and fresh weight of ginseng root varied from 1.00 to 2.33g under various conditions. The optimum pH of medium and sucrose concentration determined by a partial differentiation of the model equation, nitrogen and phosphate concentration were pH 5.6, sucrose 3.8%, nitrogen 50 mg/L and phosphate 80.7 mg/L, respectively. Under these conditions, the predicted growth of ginseng root was estimated to be 2.36g.

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In vitro Formation of Tuberous Roots from Root Ends in the Rooted Tuberous stem without shoots in Cyclamen persicum MILL.

  • Lim, Jong-Gu;Junzo, Fujigaki
    • Plant Resources
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    • v.7 no.3
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    • pp.222-225
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    • 2004
  • In Japan, propagation of cyclamen is mainly from seedlings. However, seeds are expensive and germination is slow and non..uniform. Therefore, to achieve genetically uniform propagation, multiplication must be vegetative. The rooted tuberous stems without shoots as sources of explants were cultured on the media containing BA and sucrose. After 30 days cultivation, tuberous roots were produced from the root ends attached to a tuberous stem and its capability was dependent on the type of media. The highest percentage of tuberous root formation was observed in Culture on the medium of 1/3 MS containing 0.05mgL$^{-1}$ NAA, 0.5mg L$^{-1}$ BA and 5% sucrose. Growth rates of the tuberous roots were greatly influenced by the cutting positions of a root in explants. The highest growth of was observed if small amount of root end was cut at initiation of tissue culture.

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Production of Gericudranins by Hairy Root Culture of Cudrania tricuspidata

  • Seo, Weon-Taek;Lee, In-Kyoung;Yoo, Ick-Dong;Park , Young-Hoon
    • Journal of Microbiology and Biotechnology
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    • v.5 no.4
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    • pp.234-237
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    • 1995
  • Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.

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Effect of Cytosine Arabinoside Pre-treatment in Dorsal Root Ganglion Explant Culture (Cytosine Arabinoside 전처리가 척수후근절 외식편 배양에 미치는 영향)

  • Jung, H.Y.;Han, S.;Park, J.W.;Youn, I.
    • Journal of Biomedical Engineering Research
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    • v.36 no.6
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    • pp.296-301
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    • 2015
  • Explant culture condition of dorsal root ganglion have been used to investigate the pathophysiology of peripheral nerve injury, while applying for the various clinical symptom such as trauma, pressure, and stretch. However, explant culture is usually contaminated by mitotic cells, which may observed as a newly divided cells including fibroblast or glia. The mitotic cells could be able to interrupt and change the cell signaling that make it difficult to avoid detrimental effects during the experiments. To eliminate mitotic cells, anti-mitotic reagents like mixture of uridine and 5-fluorodeoxyuridine or cytosine arabinoside were added to the cultures on the following day, but there is no research that investigate viability of anti-mitotic reagent in dorsal root ganglion explant culture. In this study, we investigate inhibition effect of cytosine arabinoside to mitotic cells in dorsal root ganglion explant culture. Also we visualized and analyzed anti-mitotic effect and toxicity of cytosine arabinoside in various concentration condition. This dorsal root ganglion explant culture condition can be applied to research that effect and mechanism of various stimulation and chemical application which affect peripheral nerve regeneration.

Auxin Affects on Production of Adventitious Roots and Secondary Metabolites in Echinacea angustifolia (오옥신 처리가 에키네시아의 부정근 및 생리활성물질 생산에 미치는 영향)

  • Jang, Young Se;Cui, Hai Yan;Lee, Eun Jung;Kim, Hae Won;Paek, Kee-Yoeup
    • Korean Journal of Medicinal Crop Science
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    • v.20 no.6
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    • pp.479-486
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    • 2012
  • The production of adventitious roots derived from root explant of Echinacea angustifolia and its secondary metabolite content were assessed in different types and levels of auxin. The induction of adventitious roots from root explant cultured in Murashige and Skoog solid medium supplemented with 1.0 mg/L indole -3-butyric acid (IBA) attained highest as 20.87 mg fresh weight and 3.07 mg dry weight per culture but root suspension culture at the same concentration of IBA enhanced biomass production as 3.07 g fresh weight and 0.38 g per culture after 4 weeks in culture. 3.0 mg/L ${\alpha}$-naphthalene acetic acid (NAA) treatment had similar effect on root biomass production as 3.07 g fresh weight and 0.38 g per culture with liquid suspension culture, whereas adventitious roots exposed to over 3.0-5.0 mg/L IBA or 5.0 mg/L NAA were less responsive by reducing the number of adventitious roots and/or changing root morphology such as short and thick. The content of secondary metabolites such as phenolic, flavonoids and total caffeic acid in adventitious roots cultured on MS medium supplemented with 1.0 mg/L IBA were attained highest as 27.20, 9.60. 10.67 mg/g dry weight, respectively. Overall, the best production of root biomass and secondary metabolites were given by 1.0 mg/L IBA.

Effects of Media and Growth Regulators on the Growth and Saponin Production of Ginseng Root (인삼 Root의 생장 및 사포닌 생성에 미치는 배지와 생장조절물질의 영향)

  • 김정혜;장은정;오훈일
    • Journal of Ginseng Research
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    • v.25 no.3
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    • pp.130-135
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    • 2001
  • Effects of media and growth regulators on the growth and saponin accumulation of ginseng root were investigated to develop the ginseng root culture system. When Panax ginseng C. A. Meyer roots were induced and cultured in various liquid media, the maximum root growth and saponin production were obtained in SH medium and an initial doubleing time of ginseng root was approximately 10 days. The patterns and contents of ginsenosides of cultured ginseng root in various media were different from each other. SH and White media resulted in higher total ginsenosides contents than the other media. Among different combinations and concentrations of growth regulators, SH medium containing 4.0mg/ L NAA gave best growth of ginseng roots, while saponin content was highest in SH medium containing 0.5mg/L BAP. These results suggested that the rapid production of ginseng saponin is possible by root culture of Panax ginseng.

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Physiological Studies on the Formation of Hairy Root by the Agrobacterium rhizogenes ; IV. Culture of Hairy Root and Survey of the Culture Condition. (Agrobacterium rhizogenes 에 의한 hairy root 형성에 대한 생리학적 연구 ; IV. Hairy root 배양 및 배양 조건에 관한 조사)

  • Hwang, Baik;An, Jun-Cheul;lee, Jae-Hyuk
    • KSBB Journal
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    • v.4 no.3
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    • pp.246-253
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    • 1989
  • Hairy roots of carrot were induced by Agrobacterium rhizogenes $A_4$ strain within about 2-4 weeks after inoculated from root disc. Early axonic culture is established in RCM agar medium and following is in MS rigid medium. After 15 days culture, the hairy roots were vigrous growth in about 10 times of initial inoculum. Anthocyanin contents of hairy roots were more than of ordinary roots. 2, 4-D ($10^{-4}mg/ l$), sucrose (5%), nitrogen source (0.03M) contained medium was optimized to growth of hairy root and contents of anthocyanin. Phenotypic alterations of leaves are observed in transformed plants and determined the transformation of hairy roots and the transformed plants by opine assay.

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Stimulation Effect of a Soft Ferrite Ceramic Powder on Growth in Plant Cell and Tissue Cultures (연자성 세라믹 분말에 의한 식물세포 및 조직의 생장촉진 효과)

  • Ahn, Jun-Cheul;Kim, Yu-Jung;Park, Chan-Young;Hwang, Baik
    • KSBB Journal
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    • v.13 no.5
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    • pp.530-534
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    • 1998
  • The addition of the ceramic powder as state of bare in culture medium has stimulated the growth of Achyranthes japonica in both the disorganized cell and the plantlet. The grwoth rate of Hyoscyamus niger adventitious root and Pylatycodon grandiflorum hairy root was enhanced up to 100 and 250%, respectively, even though Scopolia parviflora hairy root and Hyoscyamus albus adventitious root were not. The ceramic powder has enhanced the growth of H. niger adventitious root even in a test tube immersed into its culture medium to irradiate alone without any direct contact. The ceramic powder seems to have a significant role on both the growth and the physiological action of some plants.

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Saikosaponin Contents in Bupleurum falcatum Root Produced by Tissue Culture (조직배양(組織培養)한 시호근(柴胡根)의 Saikosaponin 함량(含量))

  • Jo, Pil-Hyung;Seong, Rack-Seon;Bae, Hyung-Hwa;Soh, Woong-Young;Cho, Duck-Yee
    • Korean Journal of Pharmacognosy
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    • v.21 no.3
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    • pp.205-209
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    • 1990
  • The study for the quantitative analysis of saikosaponin of Bupleurum falcatum root produced by tissue culture was attempted. The optimal concentration of 2,4-D was 0.1 mg/l for inducing the callus. The induction and growth of the adventitious root from the callus was obtained in the suspension culture containing MS basal medium supplemented with no plant growth regulators. The results of the quantitative analysis of saikosaponins by high performance liquid chromatography (HPLC) showed that the content of saikosaponin a and c was high in the one-year-old root from somatic embryos and that of saikosaponin d was remarkably high in three-months-old adventitious root from callus.

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