Production of Gericudranins by Hairy Root Culture of Cudrania tricuspidata

Production of new flavanol derivatives with cytotoxic activity, gericudranin A and B, was studied by using hairy root cultures of Cudrania tricuspidata. Schenk and Hildebrandt (SH) medium was chosen for root growth and gericudranin production. After 35 days culture in a half-strength liquid SH medium containing $30g^{glucose}$/l, hairy root growth reached $138g^{FW}$/I and gericudranin A and B were produced at concentrations of 27mg/l and 21 mg/l, respectively. It was also observed that the contents of gericudranin A and B in hairy root were eight and six times higher than those of cudraniae radix, respectively.