• 한국산림과학회지
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• 제97권3호
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• pp.249-254
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• 2008

생물반응기를 이용한 가시오갈피 부정근 배양시에 jasmonic acid를 농도별 (0, 0.01, 0.05, 0.1, 03, 0.5 mg/L)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Jasmonic acid 처리농도별 부정근의 생장은 무처리구에서 4.6 g Dry weight (DW)/L로 가장 높았으며 jasmonic acid 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B와 E의 함량은 jasmonic acid 농도가 높을수록 증가하여 0.5 mg/L 처리구에서 각각 $476.3{\mu}g/g\;DW$, $676.0{\mu}g/g\;DW$로 생산량이 가장 많았다. 반면에 eleutheroside $E_1$은 0.01 mg/L 처리구에서 $46.1{\mu}g/g\;DW$로 생산량이 가장 많았다. 배지 1L당 eleutheroside류의 총생산량은 0.01 mg/L 처리구에서 $2468.6{\mu}g/L$를 생산하여 가장 우수하였다. 0.1 mg/L의 jasmonic acid 처리 후 10일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 jasmonic acid처리 후 6일째, eleutheroside E는 처리 후 8일째, eleutheroside $E_1$은 처리 후 4일째 각각 가장 많은 생산량을 보여 주었다.

• 한국산림과학회지
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• 제99권3호
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• pp.331-336
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• 2010

생물반응기를 이용한 가시오갈피 부정근 배양시에 methyl jasmonate를 농도별 (0, 5, 10, 20, 40 및 80 ${\mu}M$)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Methyl jasmonate 처리농도별 부정근의 생장은 무처리구에서 5.4 g dry weight(DW)/L로 가장 높았으며 methyl jasmonate 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B의 함량은 methyl jasmonate 농도가 높을수록 증가하여 40 ${\mu}M$ 처리구에서 각각 359.9 ${\mu}g$/g DW로 생산량이 가장 많았다. 반면에 eleutheroside E와 $E_1$은 10 ${\mu}M$ 처리구에서 각각 798.1 ${\mu}g$/g DW, 19.7 ${\mu}g$/g DW로 생산량이 가장 많았다. 배지 1 L당 eleutheroside류의 총생산량은 10 ${\mu}M$ 처리구에서 3818.1 ${\mu}g$/L를 생산하여 가장 우수하였다. 20 ${\mu}M$의 methyl jasmonate 처리 후 15일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 methyl jasmonate처리 후 12일째, eleutheroside E는 처리 후 3일째, eleutheroside $E_1$은 처리 후 9일째 각각 가장 많은 생산량을 보여 주었다.

• 농업과학연구
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• 제37권2호
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• pp.191-197
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• 2010

The objective of this research was to determine the influence of the physical and chemical properties of root substrates used during the production of 'Maehyang' strawberry propaguleson the growth of the mother plants and the rate of daughter plant formation. Plants were cultured in plastic bags containing six different formulations of root substrates composed of: a) 50% coir dust and 50% perlite (5:5 by volume, A), b) 60% coir dust and 40% perlite (6:4, B), c) 70% coir dust and 30% perlite (7:3, C), d) 70% coir dust and 30% coconut chip (7:3 D), e) 60% coir dust and 40% coconut chip (60:40, E), or f) 50% sphagnum peat and 50% vermiculite (50:50, F). All media formulations contained a moderate level of base fertilizers. Physical and chemical properties of each formulation were determined before plant establishment and after 120 days of stock plant culture and runner production. Total porosity (TP) and container capacity (CC) of all substrate formulations were higher than 85% and 55%, respectively, allowing a suitable range of air and water holding characteristics. Formulation F provided the highest TP and CC values among the all substrate modifications evaluated. Substrate formulations A, B, C and F had higher electrical conductivity (EC) and $NO_3{^-}$-N concentrations than formulations D and E, when determined before and after plant culture. Formulations A, B, C, and F, having higher EC readings, also performed better as root substrates thanthe formulations D and E in increasing fresh and dry weights of the runners as well as the production of daughter plants per plant. The 'Maehyang' strawberry plants grown in the formulation F had the highest tissue N content, followed by those grown in substrate B, A, C, or D for 120 days after transplanting. Formulation F also facilitated accumulation of higher tissue phosphorus (P) and copper (Cu) contents compared to other treatments. Results of this experiment suggest that the chemical properties, rather than physical properties, of root substrates had a major influence on the growth of mother plants and the occurrence of healthy daughter plants during the bag-culture phase of propagation.

• 한국산림과학회지
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• 제96권5호
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• pp.539-542
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• 2007

This study was carried out to investigate the impacts of jasmonic acid (JA) on adventitious root culture of Eleutherococcus senticosus. Adventitious root of E. senticosus were treated with jasmonic acid (JA) and cultured for 30 days. JA inhibited the root growth but increased eleutherosides accumulation, total phenolic contents and antioxidant activity. Among various concentrations of JA, 1.0 mg/L JA increased the total phenolic contents in E. senticosus adventitious root to $39.81{\mu}g/g$, about 2.6 times higher than that of the control. Consequently, high accumulation of total phenolic contents led to increase the antioxidant activity to 82.41%. The antioxidant activity of control was 37.89% at $2500{\mu}g/mL$. A linear correlation ($R^2$ = 0.9937, 0.9648 and 0.9883) was also shown between antioxidant activity (at 1250, 1875, and $2500{\mu}g/mL$) and total phenolic contents of adventitious root of E. senticosus.

• International Journal of Industrial Entomology and Biomaterials
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• 제6권2호
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• pp.133-138
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• 2003

Five seed kernel namely, Neem (Azadirachta indica A. Juss.), Pongamia (Pongamia glabra L. Pierre), Tamarind (Tamarindus indica L.), Mahua (Madhuca indica Gmel.) and Shikakai (Acacia cancinna De.) were tested against hatching of eggs and larval mortality of Meloidogyne incognita causing root knot disease in mulberry along with Furadan (Carbofuran) and Bionema (a bioformulation developed from Verticillium chlamydosporium) for comparison. Results revealed that highest hush-up of hatching was observed in Neem (77.40%) and Pongamia (75.99%) seed kernel extracts at 100% concentration over the check. Similarly, highest larval mortality was observed in Neem and Pongamia by 76.00% and 74.50%, respectively at 100% concentration after 72 hrs of exposure period. Pot culture studies revealed that pre-application of seed kernel powders (20 days before inoculation of nematode) found to be more effective in controlling the root knot disease than post application. In pre application of seed kernel powders, maximum reduction of root knots was observed in case of Neem seed kernel powder (54.85%) followed by Pongamia (51.9%). Similar trend was also observed in reduction of egg masses/plant and nematode population ／250 cc soil. Rest of the seed kernel extracts was found to be less effective in suppression of hatching, enhancing the larval mortality and controlling the root knot disease. However, application of Furadan and Bionema tested for the comparison were found to be more effective than seed kernel powders. The generated information seems to be useful in developing an ecofriendly integrated approach for the control of root knot nematode disease in mulberry.

• 한국산학기술학회논문지
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• 제11권4호
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• pp.1528-1533
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• 2010

갈대뿌리를 첨가하여 효모 발효시킨 산머루주의 발효특성을 조사하였다. 산머루주 발효 과정에 갈대뿌리의 첨가는 효모의 당 소모를 촉진시켜 발효를 빠르게 진행시키는 효과가 있음을 보여주었고, 발효액의 pH의 증가와 산도의 감소를 가져와서 효모가 발효를 더 잘할 수 있도록 해주었다. 특히 갈대뿌리 2%를 첨가한 산머루주는 발효기간 4일 이내에 에탄올이 약 13% 생성되는 것으로 나타나 갈대뿌리 첨가가 발효시간을 단축하는데 큰 효과가 있음을 알 수 있었다. 또한, 갈대뿌리 2%를 첨가하여 발효시킨 산머루주의 관능이 가장 우수한 것으로 나타났다. 즉, 효모를 사용하여 산머루의 알코올 발효 시에 갈대뿌리의 첨가는 당의 소모와 에탄올 생성을 촉진하고 관능을 좋게 하는 긍정적인 효과를 갖는다는 것을 확인하였다.

• 한국자원식물학회지
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• 제24권5호
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• pp.514-520
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• 2011

본 연구는 아그로박테리움을 이용한 기능획득 일일초 모상근의 대량생산을 위한 조건 확립에 대한 것이다. 본 연구에서는, 효율적인 형질전환 일일초 모상근 생산에 있어서의 최적의 일일초 품종의 선발과 최적의 일일초 조직을 결정하였으며, 또한 다양한 배지에 있어서의 모상근 유도를 조사하였다. 최종적으로 약 2,500개의 독립적인 형질전환 일일초 모상근 line을 생산하였으며, 또한 이들을 이용하여, 대사체 연구를 위한 효율적 관리 시스템을 구축하였다. 이들 모상근 line은 일일초 인돌알칼로이드 생합성 관련 유전자의 발굴 및 기능해석에 유용하게 쓰일 것이다.

• 한국식물병리학회지
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• 제14권3호
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• pp.268-277
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• 1998

The production of Korean ginseng, one of the most important medicinal root crops, is limited by many factors including soil sickness, root rots in fields as well as during storage prior to consumption. Although much research has been conducted on the diseases in field condition, little information is available on the control of postharvest roots rots. To obtain better management strategy of postharvest root rots in ginseng, biological control using antagonistic bacteria was attempted. Of 208 bacteria obtained form suppressive soil samples, 4 were selected based on the inhibitory effect on mycelial growth of two major causal fungi for postharvest root rots in ginseng, Botrytis cinerea and Fusarium solani. The culture filtrates of these bacterial antagonists greatly inhibited the conidial germination of both pathogenic fungi and produced abnormal morphology such as swollen germ tubes in F. solani and vacuolation of nongerminated conidia in B. cinerea. The population levels of bacterial antagonists on the ginseng roots were gradually increased up to 8 days of incubation. Postharvest root rots of ginseng caused by f. solani and B. cinerea were controlled in dipping tests in the ranges of 60∼80% by antagonistic Bacillus spp. obtained from suppressive soil. These results suggest that biological control using these antagonistic bacteria would be an alternative strategy to control postharvest root rots in ginseng.

• 한국식품영양학회지
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• 제31권4호
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• pp.549-558
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• 2018

The aim of this study was to investigate the characteristics and antioxidant activities of vinegar made with Etteum bell flower root (0.5%, 1%, 1.5% and 2%) during two-step fermentation. Acetic acid was fermented at $30^{\circ}C$ for 16 days and samples of vinegar were extracted at 2, 4, 6, 8, 10, 12 and 16 days. The pH of Etteum bell flower root vinegar did not significantly differ among the samples, but the acidity increased during fermentation. Alcohol content decreased at 16 days of fermentation and less than 1% alcohol was shown in all samples after fermentation. The pure acetic acid yield was 88.85~98.97%, whereby the total phenolic compound content and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging activities increased as the ratio of the Etteum bell flower root increased. The sensory scores of vinegar fermented with 1.5% Etteum bell flower root are greater than those of vinegar prepared by other treatments. Therefore, vinegar with 1.5% Etteum bell flower root added is considered to be the most suitable for manufacturing.

• Journal of Ginseng Research
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• 제28권1호
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• pp.67-70
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• 2004

인삼 근부병의 생물학적 방제를 위해 인삼 재배토양으로부터 분리된 길항미생물 Bacillu subtilis B-4228을 사용하여 방제효과를 검정하였다. 공시 균주 B-4228과 C. destructans의 대치배양결과, C destructans의 균사가 비정상적인 형태로 팽창하였으며 강한 생장억제력을 보였다. C. destructans에 의해 오염된 이병토를 이용한 pot시험결과, 건전근율이 무처리구에서 20%였으나 공시 균주 B-4228의 처리구는 82%로 높게 나타났고, 이병율(개체 인삼에 대한 뿌리썩음 이병정도의 총합비율)은 무처리의 경우 50.4%였으나 공시 균주 B-4228의 처리구는 6.0%로 낮았다.