• Journal of the East Asian Society of Dietary Life
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• v.24 no.4
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• pp.503-513
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• 2014

This study investigated the effects of dietary consciousness of gender and generation on food intake frequency in Daegu, Sangju, Kimchen and Gumi. There was a total of 359 subjects, which consisted of elementary students, college students, and adults. The survey period was from March to May 2011, and it was a self-questionnaire by convenience sampling. The results were as follows: 1. A 9 point scale on intake frequency of 59 kinds foods was classified into 17 groups. Gender had significant differences on intake frequency of meats, beans, root vegetables, tuber vegetables, fruit vegetables, mushrooms, nuts, liquors, and cola. Sixteen food groups, except for fats, showed significant differences in intake frequency by generation. 2. Dietary consciousness consisted of 15 items, and it was divided into nutritional consciousness and meal consciousness. Meal consciousness was significantly higher in females than in males, as well as college students and adults than in elementary students. 3. Nutritional consciousness had significant effects on intake frequencies of grains, leaf vegetables, root vegetables, fruit vegetables, milk and milk products, fat and fatty oils. Meal consciousness had effects on intake of grain, leaf vegetables, root vegetables, seaweeds, teas and coffee. 4. Meal consciousness of males had significant effects on intake frequencies of grains, leaf vegetables, root vegetables, seaweeds, teas, and coffee. Elementary students showed significant effects on grains, leaf vegetables, root vegetables and seaweeds.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.164-170
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• 2017

A rapid and efficient in vitro regeneration system was established for Hibiscus syriacus L. The successful regeneration protocol employs induction of shoot organogenesis on leaf, petiole, and root explants. Among the various plant growth regulators evaluated, thidiazuron (TDZ) was the most effective for inducing rapid shoot formation. Most efficient shoot regeneration frequency was obtained from Murashige and Skoog (MS) media containing 0.01 mg/L TDZ. Regeneration efficiency was highest in the roots, and lowest in the leaves. A combination of 0.01 mg/L TDZ with benzyladenine (BAP) markedly improved the frequency of shoot differentiation from the root (up to 98%) and petiole (up to 88%) explants. Furthermore, leaf and petiole explants showed the highest frequency of shoot induction in half-strength MS media containing 0.01 mg/L TDZ and 1.0 mg/L BAP, while root explants formed the greatest number of shoots when 0.01 mg/L TDZ and 0.1 mg/L BAP were added to half-strength MS media. Although the frequency of shoot differentiation from leaf explants was only 50%, the leaf is considered the most efficient plant organ for use in tissue culture because leaves are easier to obtain than roots and petioles. Our findings show that various organs of H. syriacus can be used for plant regeneration, and the protocol developed in this study may be applicable in the horticulture industry.

• Journal of Ginseng Research
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• v.45 no.1
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• Plant Resources
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• v.7 no.1
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• pp.69-76
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• 2004

Lettuce (Lactuca sativa L.) is known to contain water-soluble substances that are biologically active. Aqueous or methanol extracts and residues from leaves of lettuce plants were assayed to determine their allelopathic effects, and the causative allelochemicals from fractions were quantified by means of HPLC analysis and bioassayed. Extracts from oven-dried leaf samples were more phytotoxic than those from freeze-dried samples. Leaf extracts of 40 g L$^{-1}$ were completely inhibitory on root growth of alfalfa (Medicago sativa L.), while root growths of barley (Hordeum vulgare L.) and soybean (Glycine max L.) were less sensitive. Early seedling growth of both alfalfa and barnyard grass (Echinochloa crus-galli) was significantly reduced by methanol leaf extracts. The major allelopathic substances analyzed by HPLC were coumarin, trans-cinnamic acid, o-coumaric acid, p-coumaric acid and chlorogenic acid. Of them p-coumaric acid was found as the greatest amount (8.9 mg 100 g$^{-1}$ ) in the EtOAc fraction; only coumarin was found in all the fractions. Hexane and EtOAc fractions of L. sativa reduced alfalfa root growth more than did BuOH and water fractions. These results suggest that lettuce had potent herbicidal activity, and that its activity differed depending on type and amount of causative compounds by fraction.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2000.05a
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• pp.38-44
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• 2000

To determine the optimum sowing date and nursery period in Alisma piantago in the southern area of Korea, Alisma plantago local cultivar was grown under three different sowing dates and nursery periods. The plant height, leaf width and leaf length at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 12.1cm, 2.5cm and 3.1cm respectively and it shows tess decrease than that of plant height, leaf width and leaf length with 12.3cm, 2.6cm and 3.2cm respectively which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows less decrease than that of plant height, leaf width and leaf length with 15.2cm, 3.1cm and 5.2cm respectively at the area sown on the seedbed in July 10 and condected the growing seedling in the same date. The stem length, number of stems and diameter of root at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 31cm, 11 and 3.7cm respectively and it shows more increase than that of stem length, number of stem and diameter of and on the other hand shows tess decrease than that of stem length, number of stems and diameter of root with 30cm, 10cm, 35cm respectively which was sown on the seedbed in 30 days, and on the other hand shows less decrease than that of stem length, number of stems and diameter of root with 32cm,13cm,3.9cm respectively at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date, The fresh yield of roots at the area sown on the seedbed in June 30 and conducted the growing seedling in 30 days is 431.4Kg/10a and it shows more increase of 26.9Kg than that of fresh yield of root with 410.59g110a which was sown on the seedbed in lune 20an4 carried out the growing seedling in 30 days, and it also shows more increase of 7Kg than that of fresh yield of root with 430.4Kg/10a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. The dry yield of roots at the area on the seedbed in June 30 and conducted the growing seedling in 30 days is 351.9Kg110a which was sown on the seedbed in June 20 and carried out the growing seedling in 30 days, and it also shows more increase of 16.4Kg than that of dry yield of root with 335.4Kg110a at the area sown on the seedbed in July 10 and conducted the growing seedling in the same date. Therefore, the seedling period of proper seedbed for high yield bumper crop of Alisma plantago for exporting to Japan is June 30 and the number of days for seedling is 30 days.

• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.354-359
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• 2010

In the present study, we investigated the chemical composition, antioxidant activities and nitrite scavenging ability in leaf and root of Youngia sonchifolia Max. The leaf powder contained 4.3% of water, 53.9% of crude carbohydrate, 21.6% of crude protein, 3.5% of crude fat and 16.7% of crude ash. The root powder contained 4.8% of water, 65.9% of crude carbohydrate, 17.4% of crude protein, 3.2% of crude fat and 8.7% of crude ash. The major mineral elements both in leaf and root powder were potassium, calcium, and magnesium. Contents of unsaturated fatty acids were higher than those of saturated fatty acids both in leaf and root powder. Total polyphenol and flavonoid contents of methanol extract in leaf were 3,922.4 mg/100 g and 1,903.2 mg/100 g respectively. In comparison, total polyphenol and flavonoid contents of methanol extract in root powder were 1,898.4 mg/100 g and 359.8 mg/100 g. The antioxidative activities of several solvents extract of leaf and root powder were investigated by measuring electron-donating ability using 1,1-diphenyl-2-picrylhydrazyl (DPPH). Of the each extracts, ethyl acetate extract of leaf and root powder showed relatively higher antioxidant activity; 94.3% in the leaf powder and 92.9% in the root powder. Nitrite scavenging ability was also highest in the ethyl acetate extract of leaf (45.4%) and root powder (28.8%). These results suggest that ethyl acetate extract of Youngia sonchifolia Max. can be used as a functional materials.

• Korean Journal of Food Science and Technology
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• v.37 no.2
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• pp.154-163
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• 2005

Chemical and flavor components of Bulro Kugi (Lycium chinense Mill) fruit leaf, and root were compared. Fructose and glucose were detected in fruit and leaf, and sucrose in root, respectively. Citrate was the highest among organic acids in fruits, and malate in leaf and root Capsaicin was detected in leaf and root. Volatile flavor compounds were extracted by simultaneous distillation and extraction method using Likens and Nickerson's extraction apparatus. Concentrated flavor extract was analysed, and 128 compounds, including 22 acids 15 alcohols, 12 aldehydes, 8 esters, 10 furans, 26 hydrocarbons, 4 phenols, 2 pyrroles, 1 pyrazine, and 28 miscellaneous components. were identified by GC and GC-MS. Main volatile compounds were hexadecanoic acid and 2-furancarboxaldehyde in hot-air dried fruit, hexadecanoic acid and 1-hexadecene in fresh fruit, 3, 7, 11, 15-tetranethyl-2-hexadecan-1-ol and hexadecanoir acid in leaf, and hexadecanoic acid in root.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.6 no.6
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• pp.25-28
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• 2003

This study was conducted to development of rapid propagation method by leaf cuttings in Hibiscus hamabo native to southern seaside of Korea, and special object of this study was to determine the effects of IAA, IBA, and NAA on rooting in leaf cuttings of H. hamabo. Rooting was promoted by dipping treated with IAA and IBA. And rooting percentage was greatest at 1,000 and 2,000$mg{\cdot}L^{-1}$ IBA. At higher concentrations of IAA and IBA, more adventitious roots were developed. Also, IAA at high concentrations (above 2,000$mg{\cdot}L^{-1}$) and IBA ranged from 500~2,000$mg{\cdot}L^{-1}$ promoted on root number and rootingratio. However, root formation in Hibiscus leaf cuttings inhibited by NAA application.

• The Plant Pathology Journal
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• v.17 no.4
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• pp.227-230
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• 2001

Bioassay techniques using young leaves and roots were developed to screen resistance of Atractylis spp. against Phytophthora drechsleri. Among 638 plants collected from various regions of Korea from 1994 to 1996, 67 were pre-screened in fields naturally infested with P. drechsleri, which is the causal pathogen of rhizome rot of Atractylis. Among the pre-screened sources, 18 (ca. 26.8%) were highly resistant to the pathogen in leaf inoculation. In the root inoculation test, abundant sporangia were formed in susceptible plant roots, while only a few or no sporangia were produced on the roots which were found resistant in the leaf inoculation test. Among the selected resistant plants, A. japonica 96066 and 96104 were used to cross with another species, A. macrocephala 96362 that showed high yield with good quality of rhizome but susceptible to the pathogen. The F$_1$hybrids designated as HA03 turned out to be resistant to the pathogen, indicating that resistant gene(s) was inherited. Among intra-species hybrids of A. japonica, HA07 and HA09 were resistant to the pathogen in leaf inoculation and moderate in root inoculation. However, HA08 was susceptible in both inoculation tests. This result suggests that the parent material might be genetically heterogeneous. Further genetic study should be carried out to verify this phenomenon.

• The Korea Journal of Herbology
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• v.34 no.1
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• pp.59-65
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• 2019

Objectives : This study reviewed the antioxidant activity of each part of Portulacae Herba (PH) in order to better analyze the possibility of PH being used as a natural material. Methods : The root, stem, and leaves of the PH were separated, dried at $50^{\circ}C$, put in a 70% ethanol and then extracted three times every six hours. Antioxidant activities was performed by measuring the total polyphenol and total flavonoid contents, DPPH, ABTS, nitrite, hydroxyl radical scavenging activity, and $Fe^{2+}$ chelating. Results : The total polyphenol and total flavonoid contents was highest in the root, then the leaves, and lastly the stem. DPPH and ABTS radical scavenging activities for the root extract indicated the highest activity followed by the leaf and stem extracts. It also showed similar activity to that of the ascorbic acid. Hydroxyl radical scavenging activity and $Fe^{2+}$ chelating activity resulted to be highest in the root extract and then the leaf and stem extracts. Nitrite scavenging activity decreased with higher pH levels, and activity was highest in the order of root extract, leaf extract, and stem extract. Also, in reaction to a solution with a pH 1.2 level, the root extract showed similar scavenging activity to that of BHT. Conclusions : Therefore, based on the above results, it was verified that the roots of PH have a high antioxidant level, and they can be considered to have potential to be applied to functional food, cosmetics, and medicine with antioxidant efficacy.