• Korean Journal of Fisheries and Aquatic Sciences
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• v.48 no.2
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• pp.178-186
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• 2015

Protease inhibitors (PI) of trypsin and papain as target proteases from the roe of bastard halibut Paralichthys olivaceus were fractionated out using ammonium sulfate precipitation (A), DEAE 650M anion exchange chromatography (D), and Sephacryl S-300 gel filtration (S). The recovery percentages of the fractions with the strongest inhibitory activity for each fractionation method were 13% for the A4 fraction, 21.2% for the D3 fraction, and 21.3% for the S2 fraction, with specific inhibitory activities of the fractions toward trypsin and casein of 168, 139, and 218 U/mg, respectively, while no inhibition of papain was observed. The $IC_{50}$ for the trypsin-specific substrate $N{\alpha}$-benzoyl-$\small{L}$-arginine-p-nitroanilide (BAPNA) was 0.65, 1.55, 2.26, and 2.85 mg/mL for the A4, S2, A3, and D3 fractions, respectively. These results suggest that chromatographic fractionation methods (D and S) based on the molecular mass and charge of the protein were more effective at fractionating PI than was ammonium sulfate precipitation based on protein solubility, and that the bastard halibut roe extract acts as a serine protease inhibitor. Therefore, the PI fraction from fish roe might be useful for inhibiting proteases in foodstuffs, and could constitute an alternative food-grade inhibitor for the surimi industry.

• Fisheries and Aquatic Sciences
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• v.19 no.5
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• pp.25.1-25.8
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• 2016

The fractionation of serine protease inhibitor (SPI) from fish roe extracts was carried out using polyethylene glycol-4000 (PEG4000) precipitation. The protease inhibitory activity of extracts and PEG fractions from Alaska pollock (AP), bastard halibut (BH), skipjack tuna (ST), and yellowfin tuna (YT) roes were determined against target proteases. All of the roe extracts showed inhibitory activity toward bromelain (BR), chymotrypsin (CH), trypsin (TR), papain-EDTA (PED), and alcalase (AL) as target proteases. PEG fractions, which have positive inhibitory activity and high recovery (%), were the PEG1 fraction (0-5 %, w/v) against cysteine proteases (BR and PA) and the PEG4 fraction (20-40 %, w/v) against serine proteases (CH and TR). The strongest specific inhibitory activity toward CH and TR of PEG4 fractions was AP (9278 and 1170 U/mg) followed by ST (6687 and 2064 U/mg), YT (3951 and 1536 U/mg), and BH (538 and 98 U/mg). The inhibitory activity of serine protease in extracts and PEG fractions from fish roe was stronger than that of cysteine protease toward common casein substrate. Therefore, SPI is mainly distributed in fish roe and PEG fractionation effectively isolated the SPI from fish roes.

• Journal of the Korean Applied Science and Technology
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• v.31 no.3
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• pp.387-393
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• 2014

In this study were performed to investigate the physicochemical properties of flying fish roe shell extract(FRSE) extracted from flying fish roe shell. FRSE was prepared using marine flying fish roe shell, and then analyzed it's physicochemical properties. The result showed that the nutritional composition of FRSE consisted of 81.00% protein, 9.12% ash and 4.48% moisture. There were OH-proline and glycine known as characteristics of collagen peptide in the amino acid analysis of FRSE, and there were large amount of glutamic acid and aspartic acid involved in the metabolism of glucose and fat. The calories of FRSE was 347 kcal/100g and molecular weight appeared less than 1,300 Da molecular weight distribution.

• Journal of the Korean Society of Food Science and Nutrition
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• v.20 no.5
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• pp.503-508
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• 1991

The salted and dried mullet(Mugil Japonicus) roe had about 42% of protein and 41% of lipid content as major compoments. Therefore the lipid oxidation and the deterioration of protein occurred during drying and storage periods. In order to keep the good quality of the dried roe, acid, peroxide, thiobarbituric acid value and the contitutional amino acid were studied in the different drying air velocities at the conditions of 60% R.H. and $20^{\circ}C$ of drying air temperature. The optimum air velocity showed 2m/sec, which was excellently superior to the lipid and protein quality than those of other air velocities on the dried mullet roe. The drying period shortened from 20 days in the conventional processing method to 7 days in the modified processing method that had 2m/sec of drying air velocity. The drying rate curve had only a falling-rate drying period and not to have a constant-rate drying period.

• International Area Studies Review
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• v.12 no.2
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• pp.149-173
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• 2008

This paper empirically studies the relationship between R&D expenditures and firms value. First, we can conjecture that R&D expenditures are enhancing the firms value. Such findings depend on an existing research, which R&D expenditures are intangible asset rather than expenses. Although, under U.S. accounting standards, financial statements do not report intangible assets but costs. Second, we can conjecture that short-term, the rate of increase in R&D expenditures had negative influence on firms valuation, because such findings indicates that R&D spending of costs incur mis-pricing. But long-term, consistently R&D expenditures may attract investors on the stock market. Third, lately firms focus on capital efficiency management, such a firms R&D expenditures incur high ROE. Generally investors put too much confidence in capital efficiency management and high ROE may attract investors on the stock market. Finally, High-Tech through the R&D investment improve firms competitive advantage, by competitive advantage, firms have reduced cost and raised productivity in the end improve firms value.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.2 no.1
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• pp.1-14
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• 2021

The study has been carried out with an objective to prepare Siberian roe deer habitat potential maps in South Korea based on three geographic information system-based models including frequency ratio (FR) as a bivariate statistical approach as well as convolutional neural network (CNN) and long short-term memory (LSTM) as machine learning algorithms. According to field observations, 741 locations were reported as roe deer's habitat preferences. The dataset were divided with a proportion of 70:30 for constructing models and validation purposes. Through FR model, a total of 10 influential factors were opted for the modelling process, namely altitude, valley depth, slope height, topographic position index (TPI), topographic wetness index (TWI), normalized difference water index, drainage density, road density, radar intensity, and morphological feature. The results of variable importance analysis determined that TPI, TWI, altitude and valley depth have higher impact on predicting. Furthermore, the area under the receiver operating characteristic (ROC) curve was applied to assess the prediction accuracies of three models. The results showed that all the models almost have similar performances, but LSTM model had relatively higher prediction ability in comparison to FR and CNN models with the accuracy of 76% and 73% during the training and validation process. The obtained map of LSTM model was categorized into five classes of potentiality including very low, low, moderate, high and very high with proportions of 19.70%, 19.81%, 19.31%, 19.86%, and 21.31%, respectively. The resultant potential maps may be valuable to monitor and preserve the Siberian roe deer habitats.

• Fisheries and Aquatic Sciences
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• v.25 no.7
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• pp.390-402
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• 2022

In this study we investigate physical and chemical characteristics of immature and mature skipjack tuna (Katsuwonus pelamis) roes in fresh and dried forms. Fresh roes were studied for histological structure and also dried by three methods: hot air drying (HD), vacuum drying (VD) and freeze drying (FD). The obtained roe powders were analysed for proximate composition, color value, fatty acid composition, amino acid profile, equivalent umami concentration (EUC) and protein pattern. Unyolked oocytes were more common in immature roes, while fully yolked oocytes were more common in mature roes. All dried tuna roes contained high content of protein and lipid (69.31%-70.55% and 11.14%-16.02%, respectively). The powders obtained by FD provided the highest lightness value (L^*). The main fatty acid found in all roe powders was docosahexaenoic acid (DHA) (23.49%-27.02%). Glutamic acid, leucine, and aspartic acid were the three most abundant amino acids found in the powders (13.58-14.61, 8.06-8.42, and 7.81-8.39 g/100 g of protein, respectively). The mature roe powder obtained from HD provided the highest EUC value (73.09 g monosodium glutamate/100 g of samples). The protein band at molecular weight of 97 kDa (vitelline) represented the major protein. Therefore, dried tuna roe could be a functional ingredient source of protein and lipid rich in DHA and it also has potential to be used as taste enhancer with umami compound.

• Culinary science and hospitality research
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• v.22 no.6
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• pp.52-60
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• 2016

This study attempts to measure the financial performance of the food distribution company. In order to achieve the goal, this study have measured the ratios of ROE, ROA applying the DuPont analysis, which have been demonstrated with tables to show the change periodically. DuPont analysis is based on analysis of Return on Equity (ROE) & Return on Investment (ROI). The return on equity disaggregate performance into three components: Net Profit Margin, Total Asset Turnover, and the Equity Multiplier. The return on investment consists of Assets Turnover (Operating Income${\times}$Total Assets) and Profit Margin (EBIT${\times}$Operating Income). From the study it if found that Hyundae Green Food's Financial performance is high followed by Foodmerce and then Dongwon home food and Lotte Food. The four companies are significant at their level. In conclusion, ROE & ROI is the most comprehensive measure of profitability of a firm. It considers the operating and investing decisions can be made as well as the financing and their leverage-related decisions.

• 한국전산유체공학회:학술대회논문집
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• 2005.10a
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• pp.145-150
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• 2005

Generally, Compressible Navier-Stokes codes are used to solve high mach number flows. But, Most of high mach number flows embrace low mach number flows. This phenomenon results in low convergence rate and non-physical solution in CFD analysis. So Many researchers developed preconditioning technique to solve these problems. This Study presents how to modify previous compressible N-S computer code with little changes of structure into preconditioned compressible N-S code applying Roe's Approximate Riemann Solver. And this study show developed preconditioning code is very well operated at all mach number flows.

• Asian Journal of Business Environment
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• v.6 no.1
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• pp.5-12
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• 2016

Purpose - The purpose of this study is to investigate the effect of corporate governance practices such as (board size, board composition, CEO duality and audit committee) on the performance of selected Pakistani firms. Research design, data, and methodology - This study examines corporate governance structure by using the data of 80 non-financial firms listed on Karachi Stock Exchange Pakistan during 2010-2014. Hypotheses of the study were tested by using both descriptive and inferential statistics. Result - The findings indicate that board size and audit committee is positively related to the firm performance (ROA & ROE). In contrast, board composition and CEO duality are negatively related to the firm performance (ROA & ROE). As far as controlling variables is concerned, leverage is negative, whereas firm size is positively related to all measures of performance. Conclusions - Empirical findings concluded that corporate governance practices affect the firm performance. Therefore, it is suggested that managers should understand the governance mechanisms to work more efficiently in the firm.