• Applied Biological Chemistry
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• v.22 no.4
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• pp.191-197
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• 1979

${\beta}-Tyrosinase$ was purified and crystallized from cells of Escherichia intermedia A-21 grown in a medium supplemented with 0.2% L-tyrosine. Molecular weight of its subunit, Km value and absorption spectra were determined. Crystallization methods were also studied to eliminate any unnecessary procedures. The results obtained were as follows: 1. The purification procedure included ammonium sulfate fractionation, dialysis against potassium phosphate buffer, pH 6.0 and pH 7.0, and DEAE-Sephadex column chromatography. In the column chromatography, 11 mg of protein was applied per ml of DEAE-Sephadex for efficiency. 2. Steps of protamine sulfate treatment and Sephadex G-150 gel filtration could be eliminated for this enzyme from the known procedures. 3. The purified enzyme was dissolved in 0.01M potassium phosphate buffer containing 2-mercaptoethanol, with a concentration of 20mg/ml. Crystalline enzyme, which appears as hexagonal rods, was obtained by adding solid fine powdered ammonium sulfate to the enzyme solution. 4. Absorption maxima of the enzyme appeared at 340 and 430nm when associated with pyridoxal phosphate. 5. Km value of the enzyme for L-tyrosine was $2.31{\times}10^{-4}M$ and the molecular weight of its subunit was determined by SDS-polyacrylamide electrophoresis to be approximately 50,000.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.7 no.2
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• pp.113-119
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• 2006

Brazing is an important manufacturing process in the fabrication of Heavy Water Reactor fuel rods, in which bearing and spacer pads are joined to Zircaloy-4 cladding tubes. The physical vapor deposition(PVD) technique is currently used to deposit metallic Be on the surfaces of pads as a filler metal. Amorphous Zr-Be binary alloys which are manufactured by rapid solidification process are under developing to substitute the conventional PVD-Be coating. In the present study, brazed joint with PVD and amorphous alloys of $Zr_{1-x}Be_{x}(0.3{\le}x{\le}0.5)$ as filler metals are compared by mechanism, microstructure and hardness. The thickness of brazed joint with amorphous alloys became much smaller than that of PVD-Be. The erosion of base metal did not occur in the brazed joint with amorphous alloys. The brazing mechanism for PVD-Be seems to be Be diffusion into Zr-4 with capillary action resulting from eutectic reaction while that for amorphous alloys are associated with the liquid phase formation in the brazed joint. The brazed joint microstructure with PVD-Be consists of dendrite while that with amorphous alloys is globular. The $Zr_{0.7}Be_{0.3}$ alloy shows the smooth interface with little erosion in the base metal and is recommended a most suitable brazing filler metal for Zircaloy-4.

• Korean Journal of Materials Research
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• v.21 no.12
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• pp.697-702
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• 2011

Two-dimensional (2D) nano patterns including a two-dimensional Bravais lattice were fabricated by laser interference lithography using a two step exposure process. After the first exposure, the substrate itself was rotated by a certain angle, $90^{\circ}$ for a square or rectangular lattice, $75^{\circ}$ for an oblique lattice, and $60^{\circ}$ for a hexagonal lattice, and the $90^{\circ}$ and laser incident angle changed for rectangular and the $45^{\circ}$ and laser incident angle changed for a centered rectangular; we then carried out a second exposure process to form 2D bravais lattices. The band structure of five different 2D nano patterns was simulated by a beam propagation program. The presence of the band-gap effect was shown in an oblique and hexagonal structure. The oblique latticed ZnO nano-photonic crystal array had a pseudo-bandgap at a frequency of 0.337-0.375, 0.575-0.596 and 0.858-0.870. The hexagonal latticed ZnO nano-crystallite array had a pseudo-bandgap at a frequency of 0.335-0.384 and 0.585-0.645. The ZnO nano structure with an oblique and hexagonal structure was grown through the patterned opening window area by a hydrothermal method. The morphology of 2D nano patterns and ZnO nano structures were investigated by atomic force microscopy and scanning electron microscopy. The diameter of the opening window was approximately 250 nm. The height and width of ZnO nano-photonic crystals were 380 nm and 250 nm, respectively.

• Journal of the Korean Society for Nondestructive Testing
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• v.23 no.3
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• pp.227-236
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• 2003

Leaks in underground pipelines can cause social, environmental and economical problems. One of relevant countermeasures against leaks is to find and repair of leak points of the pipes. Leak noise is a good source to identify the location of leak points of the pipelines. Although there have been several methods to detect the leak location with leak noise, such as listening rods, hydrophones or ground microphones, they have not been so efficient tools. In this paper, acoustic emission (AE) sensors and accelermeters are used to detect leak locations which could provide all easier and move efficient method. Filtering, signal processing and algorithm of raw input data from sensors for the detection of leak location are described. A 120m-long pipeline system for experiment is installed and the results with the system show that the algorithm with the AE sensors and accelerometers offers accurate pinpointing of leaks. Theoretical analysis of sound wave propagation speed of water in underground pipes, which is critically important in leak locating, is also described.

• Journal of Energy Engineering
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• v.13 no.4
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• pp.242-258
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• 2004

The duplex burnable poison absorbers concept was suggested by Korea Atomic Energy Research Institute. This BP rod is composed of inner region of natural U-Gd$_2$O$_3$ and outer shell of enriched UO$_2$-Er$_2$O$_3$. It is expected that this burnable absorber has same reactivity control capability with gadolinia burnable absorber used in extened fuel cycle. In order to evaluate the nuclear feasibility of duplex BPs, the nuclear design characteristics were compared with that of four types of burnable absorbers; gadolinia, erbia, IFBA, dysprosia duplex BP on 24 months fuel cycle for Korean Standard Nuclear Power plants. According to the evaluation results of nuclear characteristics, the duplex BPs were better than other BPs on k-infinitives, reactivity holddown worth (RHW), pin power peaking and moderator temperature coefficient (MTC). The possibility of nuclear core design was also confirmed based on the optimized fuel assemblies which were searched for a sensitivity analysis. Characteristics of core design with duplex BPs was compared with that of reference core with gadolinia BPs for cycle length, power peaking and MTC. The duplex BP core had a little longer cycle length by 4 to 7 days because of increased amount of fissile in enriched uranium at the outer shell of duplex BP In case of power peaking F$\_$Q/ of duplex BP core was reduced from 1.5773 to 1.5335. MTC was also less -0.48 pcm/C than that of reference core. Finally, evaluation of fuel cycle economy was performed for the manufacturing feasibility test and fuel cost evaluation with duplex BPs. Fuel cycle economy of duplex BP core almost was equivalent with that of gadolinia BP core.

• Nuclear Engineering and Technology
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• v.23 no.1
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• pp.1-11
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• 1991

The objective of this study is to investigate the effect of weld line on the mechanical strengths and the process of weld line elimination in the Zircaloy-4 resistance upset welding for the fabrication of heavy water reactor fuel rods. The weld current and the amount of upset increased linearly with the main heat, in which two relations between them were derived. It was found that the threshold to obtain sound weld was 50% of main heat in terms of weld upset size, mechanical strengths and weld line elimination. The weld microstructure of resistance upset welds of Zircaloy-4 comprsied basketweave, Widmanstatten and martensite respectively by changing the main heats. Dimples on uniaxially fractured surface at weld line in the Zr-4 welds were larger and deeper compared with those on biaxially fractured surface. It was also found that the process of the weld line elimination in the resistance upset weld of Zircaloy-4 could be divided into three stages in terms of the presence of many pores, their shrinkage and elimination, and the shrinkage of the original weld interface with increasing weld currents.

• Journal of the Korea Institute of Building Construction
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• v.19 no.1
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• pp.9-18
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• 2019

Concrete, which is the most widely used building material in modern times, has been improved not only in strength but also in structural performance such as increase in toughness and ductility, weight reduction, and improvement in quality of human life. Due to the surge in demand for the building, there is a tendency to be used variously from architectural panel and architecture to interior accessories. In Korea, a light-transmitting concrete, LEFC(Light Emotion Friendly Concrete), that insert plastic rods to stimulate emotional sensation through the combination of light and concrete has developed. In previous research, it was confirmed that the use of a synthetic foam agent rather than an animal foam agent did not cause a fogging phenomenon. In this study, lightweight by applying foaming agent to LEFC and two types of fiber (Nylon Fiber, Polyvinyl Alcohol) were compared to achieve to investigate the fiber to be applied in future. An equation that can predict the loss and adhesion reduction of the concrete section according to the diameter of the rod (5mm, 10mm) and the interval (10mm, 15mm, 20mm) was proposed.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.17 no.2
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• pp.167-181
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• 2019

Currently, the development of evaluation technology for vibration and shock load characteristics and spent nuclear fuel structural integrity under normal conditions of transport is being conducted in the Republic of Korea. This is the first such research conducted in the Republic of Korea and, thus, previous international studies need to be investigated and will be referred to in the ongoing project. Before 2000, several studies related to measurement of vibration and shock loads on spent nuclear fuel were conducted in the US. US national research institutes conducted uniaxial fuel assembly shaker tests, concrete block tests, and multi-axis fuel assembly tests between 2009 and 2016. In 2017, multi-modal transportation tests including road, sea, and rail transport were also performed by research institutes from the US, Spain and the Republic of Korea. Therefore, test preparation procedures, acceleration and strain measurement results, and finite-element and multi-body dynamics analysis were investigated. Based on the measured strain data, the preliminary conclusion was obtained that the measured strain was too small to cause damage to spent nuclear fuel rods. However, this conclusion is a preliminary conclusion that only reviews part of the results; a detailed review is being conducted in the US. The investigation of international studies on spent nuclear fuel structural integrity tests considering vibration and shock loads under normal conditions of transport in the US will be useful data for the project being conducted in the Republic of Korea.

• Journal of Korean Tunnelling and Underground Space Association
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• v.21 no.3
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• pp.397-418
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• 2019

It is very important to understand and analyze the interactive behaviour between ground and adjacent structures due to tunneling. With many technological advancement in modern society, numerous methods for analyzing the interactive behaviour are used in a wide range of civil engineering fields. Close range photogrammetry is mainly being used in the field of geotechnical engineering and research on measuring methods associated with GeoPIV has been currently increased. Originally, the close range photogrammetry using target points and aluminum rods for VMS (Vision Measurement System) program has been used. However, applying this has a problem that external errors can be occurred because the target points are artificially installed by hand, and if the grid between points is being wider or narrower, deficient data can be obtained. Therefore, in this study, MATLAB-based No-target program that can analyze displacement without using target was developed. Additionally, this study focused on comparison and verification with existing program through numerical analysis and laboratory model test. Three cases of Greenfield condition, Strip foundation, and Pile foundation were analyzed. From results of VMS program and No-target program, the error rate and reliability of the total displacement and the vertical displacement were analyzed. It was also compared and verified through the finite element numerical program, PLAXIS.

• Korean Journal of Clinical Laboratory Science
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• v.51 no.2
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• pp.155-163
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• 2019

During the time period from 2010 to 2017, out of 162,551 blood specimens, 11,233 (6.9%) specimens were positive for culture and 11,865 strains were cultured. Among the isolates, 47.8% were Gram positive cocci, 38.8% were Gram negative rods, 4.2% were Gram positive bacilli, 6.8% were fungi and 2.3% were anaerobes. When the culture results were compared according to gender, 55.0% (2,732/4,969) of the isolates were found in males and 45.0% (2,237/4,969) were isolated in females. In addition, when categorized according to age group, people in their 70s were the most separated by 28.7% (1,426/4,969) and this showed a great difference from 1.2% (62/4,969) of people in their teens. MRSA decreased significantly from 66.7% in 2016 to 46.8% in 2017. The vancomycin resistance rate of E. faecium was 35.0% (48/137). The ESBL positive rate of E. coli in intestinal bacteria was increased from 17.2% in 2010 to 28.8% in 2017, but the positive rate decreased for K. pneumoniae. 11.8% (14/119) of multidrug-resistant P. aeruginosa (MDRPA) of P. aeruginosa and 64.3% (161/252) of MDRAB of A. baumannii showed high resistance. Because the microbial susceptibility and antimicrobial susceptibility patterns of the blood specimens isolated from all the blood specimens differ according to the time period, region and patients, periodic analyses of different pathogens and understanding the changes in the degree of susceptibility to antimicrobial susceptibility have been conducted in hospitals.