• Mycobiology
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• 제45권4호
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• pp.421-425
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• 2017

In 2016, grape fruits showing ripe rot symptom were found in fields of Korea. The fungus was isolated and identified as Colletotrichum viniferum based on morphological characteristics and nucleotide sequence data of the internal transcribed spacer, glyceraldehyde-3-phosphate dehydrogenase and ${\beta}$-tubulin. To our knowledge, this is the first report of C. viniferum causing grape ripe rot disease of grape fruits in Korea.

• The Plant Pathology Journal
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• 제19권5호
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• pp.227-230
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• 2003

Ripe rot was commonly found in overripe kiwifruits (Actinidia deliciosa) during a disease survey conducted in Jeju and Jeonnam provinces in Korea in 1999. While the disease did not manifest any clear external symptoms on kiwifruits, it caused portion of the fruit surface to collapse. Watersoaked flesh tissue could be seen on the sunken part when the skin of the collapsed portion was peeled off. The milky internal symptom with dark green margin developed concentrically as the fruit ripened. A species of Botryosphaeria was consistently isolated from lesions showing typical symptoms of ripe rot on kiwifruit, and its pathogenicity was confirmed by artificial inoculation test on healthy kiwifruits. The fungus was also pathogenic to apple and pear. The mycological characteristics of the fungus were consistent with those of Botryosphaeria dothidea. This is the first report on the detailed mycological characteristics of the causal organism of post-harvest ripe rot of kiwifruit in Korea.

• 한국응용곤충학회지
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• 제1권
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• pp.47-50
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• 1962

만부병은 포도의 착색기부터 장마가 오면 격발하는 병해로서 우리나라에 있어서 피해가 심함은 물논 방제가 어려운 것으로서 본 시험은 피대와 약제살포를 겸하는 것과 약제살포만을 하여 그 효과를 비교코저 원예시험장 과수과 포도원에서 품종 켐벨얼리를 공시하여 시험하였다. L 유대구는 무대구보다 약제간이나 무처리간에 있어서 포도만부병을 방제하는데 효과적 이였다. 2. Tuzet와 Delan은 유대구나 무대구에 있어서 다른 처리구보다 포도만부병방제에 효과적이었다

• The Plant Pathology Journal
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• 제37권4호
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• pp.347-355
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• 2021

Ripe rot caused by Botryosphaeria dothidea is one of the serious diseases of postharvest kiwifruit. In order to control ripe rot on Actinidia chinensis cultivar 'Zesy002', several commercial agrofungicides were selected by an antifungal test on an artificial medium. Furthermore, disease suppression by the selected fungicides was evaluated on the kiwifruit by inoculation with a conidial suspension of B. dothidea. On the artificial media containing boscalid + fludioxonil was shown to be the most effective antifungal activity. However, in the bio-test pyraclostrobin + boscalid and iminoctadinetris were the most effective agrochemicals on the fruit. On the other hand, the infection structures of B. dothidea on kiwifruit treated with pyraclostrobin + boscalid were observed with a fluorescent microscope. Most of the fungal conidia had not germinated on the kiwifruit treated with the agrochemicals whereas on the untreated fruit the fungal conidia had mostly germinated. Electron microscopy of the fine structures showed morphological changes to the conidia and branch of hyphae on the kiwifruit pre-treated with pyraclostrobin + boscalid, indicating its suppression effect on fungal growth. Based on this observation, it is suggested that ripe rot by B. dothidea may be suppressed through the inhibition of conidial germination on the kiwifruit treated with the agrochemicals.

• 식물병연구
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• 제7권3호
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• pp.140-144
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• 2001

1997년부터 1999년까지 켐벨얼리품종을 대상으로 대구, 영천, 김천지방에서 탄저병의 발생소장을 조사한 결과 7월과 8월의 강우와 상관이 있었으며 7월 하순부터 초발 되었다. 과방에 봉지를 씌워 탄저병의 주 감염시기를 조사하였을 때 7월 25일 이후 봉지를 씌운 과방에서 발병이 되었으며 시기별로 외관상 건전한 과방을 습실처리하여 잠복감염을 조사하였을 때 7월 25일 이후에 수확된 과일부터 발병 되었다. 탄저병 포자현탁액을 포도표면에 접종하고 균사의 발달을 관찰한 결과 24시간에 부착기가 관찰되었으며 48시간후에는 전면이 균사로 덮여졌다. 접종후 경과시간별 발병정도는 24시간이상 발병조건 부여시 발병이 되었다. 포도 탄저병 방제방법으로는 타로닐 ·마이탄 수화제를 7월 20일 경부터 4회살포시 높은 방제가를 보였다.

• 식물병연구
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• 제24권2호
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• pp.113-122
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• 2018

Ripe rot, Botrytis storage rot and Sclerotinia rot are major fungal diseases that lead to deterioration of fruit quality in Korea. Ripe rot, which is caused by Botryosphaeria dothidea, affects harvested fruits during post-storage ripening, while Botrytis storage rot, caused by Botrytis cinerea, affects harvested fruits during cold storage, and Sclerotinia rot, caused by Sclerotinia sclerotiorum, mainly affects immature fruits on the trees. Major fungal fruit rots tend to affect yellow- and red-fleshed cultivars of kiwifruit more severely because of the sharp increase in their cultivation acreage in recent years in Korea. In this review, we summarize symptoms and epidemiological characteristics of the major fungal fruit rots and propose integrated management methods of the diseases that can be practically utilized at the farmers' orchards in order to prevent the diseases based on our research works and field experiences and the research works of others conducted during the last three decades worldwide.

• The Plant Pathology Journal
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• 제24권3호
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• pp.269-278
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• 2008

Ripe rot was frequently observed on fruits, leaves and stems of grape growing in eight locations in Korea from 2004 to 2006. All 30 isolates of Colletotrichum sp. were obtained from lesions of the ripe rot on grape plants. Out of the isolates, 19 isolates were identified as Colletotrichum acutatum and the others as Colletotrichum gloeosporioides based on morphological and cultural characteristics. Inter and intra specific variations of the Colletotrichum spp. isolates were investigated using RAPD and sequences of rDNA ITS and $\beta$-tubulin-2. Isolates of C. acutatum and C. gloeosporioides were distinctly differentiated by molecular analyses. Phylogenetic trees of ITS and$\beta$-tubulin-2 showed that Korean isolates of C. acutatum were clustered into groups A2 and A3 among the eight global groups. A2 included non-chromogenic isolates and A3 chromogenic ones. Both C. acutatum and C. gloeosporioides isolates were tested for pathogenicity to grape leaves. All isolates tested induced lesions on the leaves of grape by artificial inoculation. There was no difference in pathogenicity between C. acutatum and C. gloeosporioides isolates. This is the first report that C. acutatum except C. gloeosporioides causes grape ripe rot in Korea.

• 식물병연구
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• 제20권4호
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• pp.275-282
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• 2014

본 연구에서는 국내에서 가장 많이 생산, 소비되는 '캠벨얼리' 포도의 수확 후 저장과정에서 발생하는 썩음병의 종류, 병원균, 그리고 발병률을 조사하였다. 3곳의 국내 주요 포도 재배지역에서 생산되어 포장을 마친 '캠벨얼리'를 2주간 냉장보관하고, 4주간 상온저장 후 발생한 썩음병 발병률을 조사하였다. '캠벨얼리' 저장 중 발생하는 주요 썩음병은 잿빛곰팡이병, 푸른곰팡이병, 만부병, 새 썩음병 1, 새 썩음병 2이었다. 잿빛곰팡이병, 푸른곰팡이병, 만부병의 병징으로부터 분리한 병원균은 각각 Botrytis cinerea, Penicillium sclerotiorum, Colletotrichum acutatum으로 동정되었으며, 새 썩음병 1과 새 썩음병 2에서 분리한 병원균은 아직 정확한 동정 중이다. 국내 주요 포도 재배지역 3곳에서 생산된 포도에서 발생한 각 병의 발병률은 새 썩음병 1과 새 썩음병 2가 높았으며, 만부병이 가장 낮았고, 잿빛곰팡이병과 푸른곰팡이병의 발생도 낮았다. 또한 각 병의 발병은 재배 지역이 다른 포도에서 상당한 발병률의 차이를 보였다.

• 식물병연구
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• 제9권4호
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• pp.196-200
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• 2003

우리나라 참다래 주요 재배지인 전남과 경남 그리고 제주지역의 16개 포장에서 임의로 수집한 1600개 참다래 과실의 평균 저장병 발병율은 32.0%로 나타났지만, 저장병 발병율은 수확한 과수원에 따라서 5%에서 68%까지 다양했다. 저장병에 걸린 참다래 과실 중에서 15.4%가 외부 병징, 68.4%가 내부 병징을 나타내었고, 외부와 내부 모두에 병징을 나타내는 것은 16.2%였다. 저장병에 걸린 과실들에서 병원균의 검출율은 분리한 지역에 따라 차이가 있었으며, Botryosphaeria dothidea가 83.3%, Diaporthe actinidiae는 11.9%, Botrytis cinerea가 1.4%의 검출율을 보여 주요 저장병원균으로 밝혀졌다. 참다래 주요 저장병원균으로 확인된 B. dothidea와 D. actinidiae에 의해 발생하는 참다래 주요 저장병을 과실에 나타난 각 병징과 각 병원균들의 특성을 고려하여 과숙썩음병과 과실꼭지썩음병으로 각각 구분하여 명명하고자 한다.

• The Plant Pathology Journal
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• 제26권1호
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• pp.49-56
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• 2010

Thirty-six isolates of Colletotrichum spp. were obtained from infected grapes in two different locations of Korea; 18 isolates from Cheonahn, where carbendazim (MBC) and the mixture of MBC and diethofencarb (NPC) had been applied to control grape ripe rot, and 18 isolates from Cheongju, where no fungicides had been used. Sequences analysis of the internal transcribed spacer (ITS) and the $\beta$-tubulin gene identified 34 of the 36 isolates as Colletotrichum gloeosporioides. The remaining two isolates from Cheongju were identified as C. acutatum. Of the 18 isolates from Cheonahn, 12 were resistant to both MBC and the mixture (MBC+NPC), and six were sensitive to them. All C. gloeosporioides isolates from Cheongju, but not the two C. acutatum isolates, were sensitive to these fungicides. Sequence analysis of the $\beta$-tubulin gene in all isolates revealed that C. gloeosporioides resistant to MBC and MBC+NPC had a tyrosine instead of phenylalanine at the amino acid position 200. The appearance of resistance to MBC and the mixture in C. gloeosporioides correlated with the history of fungicide application in Korea.