• 한국자원식물학회지
• /
• 제29권4호
• /
• pp.504-510
• /
• 2016

본 연구는 중간 신육형 직립 초형 동부 계통의 파종기 이동이 생태반응과 생육 및 수량에 미치는 영향을 구명하고자 전남 나주(위도 35° 04' N, 경도 126° 54' E)에서 2013년과 2014년에 수행하였다. 시험계통으로 전남1호와 전남2호(옥당)를 이용하여 4월 중순부터 8월 중순까지 1개월 간격으로 5회 파종하였다. 출현일수는 4월 중순 파종에서 12일로 길었고, 기타 파종기에서는 5∼3일이었다. 개화일수는 4월 중순부터 7월 중순까지는 파종기가 지연됨에 따라 짧아지다 8월 중순 파종에서 길어졌다. 즉, 4월 중순 파종에서 62일 내외로 가장 길었고, 7월 중순 파종에서 35일로 가장 짧았다. 개화시에서 수확기까지의 소요일수는 4월 중순부터 7월 중순 파종에서 17∼15일로 짧았으나, 8월 중순 파종에서는 24일로 긴 편이었다. 경장은 5월 중순과 6월 중순 파종에서 긴 편이었고, 화경장은 4월 중순 파종에서 긴 편이었으며, 주경절수는 7월 중순 파종, 분지수는 4월 중순 파종에서 많았다. 전남1호와 전남2호(옥당)의 10 a당 수량은 4월 중순 파종에서 각각 199, 211 ㎏로 가장 많았고, 다음으로 7월 중순 파종에서 각각 191, 195 ㎏로 많았다.

• 한국의학물리학회지:의학물리
• /
• 제27권1호
• /
• pp.37-45
• /
• 2016

양성자 치료 시 양성자 빔의 특성을 이용하여 치료 부위에 국부적인 선량을 부여하고 정상조직에 불필요한 선량을 줄이기 위해서는 인체 내 양성자 빔의 비정을 실시간으로 확인하는 것이 중요하다. 이를 위해 본 연구팀은 24개의 섬광검출기 배열 및 24채널의 신호 처리 시스템으로 구성된 즉발감마선 카메라 모듈을 개발하고 있다. 본 연구에서는 다채널의 섬광 검출기 신호를 처리하기 위하여 이중모드 다채널 신호 처리 모듈을 개발하여 그 성능을 평가해보았다. 성능을 평가한 결과 에너지 교정 모드를 통해 다채널의 섬광검출기에 대하여 동시에 에너지 교정이 가능함을 확인하였고, 이를 통하여 정확하게 3 MeV에 해당하는 측정 하한 값을 결정할 수 있었다. 고속 데이터 획득 모드를 통해 45 MeV 양성자 빔에서 발생한 즉발감마선 분포를 측정한 결과 $3{\times}10^9$개의 양성자 빔에서도 양성자 선량 분포와 유사한 결과를 얻을 수 있었고, 빔 비정을 평가한 결과 $17.13{\pm}0.76mm$로 EBT film을 통하여 측정한 비정인 16.15 mm와 굉장히 밀접한 관련이 있음을 확인하였다.

• Parasites, Hosts and Diseases
• /
• 제51권6호
• /
• pp.719-726
• /
• 2013

Mitochondrial genomes have been extensively studied for phylogenetic purposes and to investigate intra- and interspecific genetic variations. In recent years, numerous groups have undertaken sequencing of platyhelminth mitochondrial genomes. Haplorchis taichui (family Heterophyidae) is a trematode that infects humans and animals mainly in Asia, including the Mekong River basin. We sequenced and determined the organization of the complete mitochondrial genome of H. taichui. The mitochondrial genome is 15,130 bp long, containing 12 protein-coding genes, 2 ribosomal RNAs (rRNAs, a small and a large subunit), and 22 transfer RNAs (tRNAs). Like other trematodes, it does not encode the atp8 gene. All genes are transcribed from the same strand. The ATG initiation codon is used for 9 protein-coding genes, and GTG for the remaining 3 (nad1, nad4, and nad5). The mitochondrial genome of H. taichui has a single long non-coding region between trnE and trnG. H. taichui has evolved as being more closely related to Opisthorchiidae than other trematode groups with maximal support in the phylogenetic analysis. Our results could provide a resource for the comparative mitochondrial genome analysis of trematodes, and may yield genetic markers for molecular epidemiological investigations into intestinal flukes.

• 한국임상수의학회지
• /
• 제21권2호
• /
• pp.102-108
• /
• 2004

Orf virus (ORFV), a member of genus Parapoxvirus (family-Poxviridae), a causative agent of contagious ecthyma in sheep and goat leading to a condition commonly known as vesicular dermatitis. Recently, twelve goats from Iksan in Jeonbuk province were observed with clinical signs like necrotic vesicular lesions around the mucosa of mouth, nasal cavity, eye, ear, teats, abdomen and groin. Based on these clinical symptoms, contagious ecthyma infection was suspected. The skin scrapping was collected from lesions for isolation of DNA and subsequent PCR amplification of ORFV specific 235 bp region of B2L gene. All of the samples were found positive by PCR analysis. Sequencing and further phylogenetic analysis of the PCR product revealed 100% identity to Japan isolate of ORFV (Okinawa, GenBank accession number AB080769), and showed 99.6% of similarity to New Zealand strain (NZ-2, GenBank accession number U06671). It was concluded that ORFV strain detected in the present study is homologous to Japan isolate and New Zealand strain. The PCR test based on amplification of B2L gene is a highly useful tools for rapid and specific diagnosis of contagious ecthyma.

• Journal of Korean Neurosurgical Society
• /
• 제46권5호
• /
• pp.479-483
• /
• 2009

Objective : A mouse model of spinal cord injury (SCI) could further increase our basic understanding of the mechanisms involved in injury and repair of the nervous system. The purpose of this study was to investigate whether methods used to produce and evaluate photochemical graded ischemic SCI in rats, could be successfully adapted to mice, in a reliable and reproducible manner. Methods : Thirty female imprinting control region mice (weighting 25-30 g, 8 weeks of age) were used in this study. Following intraperitoneal injection of Rose bengal, the translucent dorsal surface of the T8-T9 vertebral laminae of the mice were illuminated with a fiber optic bundle of a cold light source. The mice were divided into three groups; Group 1 (20 mg/kg Rose bengal, 5 minutes illumination), Group 2 (20 mg/kg Rose bengal, 10 minutes illumination), and Group 3 (40 mg/kg Rose bengal, 10 minutes illumination). The locomotor function, according to the Basso-Beattie-Bresnahan scale, was assessed at three days after the injury and then once per week for four weeks. The animals were sacrificed at 28 days after the injury, and the histopathology of the lesions was assessed. Results : The mice in group 1 had no hindlimb movement until seven days after the injury. Most mice had later recovery with movement in more than two joints at 28 days after injury. There was limited recovery of one joint, with only slight movement, for the mice in groups 2 and 3. The histopathology showed that the mice in group 1 had a cystic cavity involving the dorsal and partial involvement of the dorsolateral funiculi. A larger cavity, involving the dorsal, dorsolateral funiculi and the gray matter of the dorsal and ventral horns was found in group 2. In group 3, most of the spinal cord was destroyed and only a thin rim of tissue remained. Conclusion : The results of this study show that the photochemical graded ischemic SCI model. described in rats, can be successfully adapted to mice, in a reliable and reproducible manner. The functional deficits are correlated an increase in the irradiation time and, therefore, to the severity of the injury. The photothrombotic model of SCI, in mice with 20 mg/kg Rose bengal for 5 minutes illumination, provides an effective model that could be used in future research. This photochemical model can be used for investigating secondary responses associated with traumatic SCI.

• 대기
• /
• 제25권2호
• /
• pp.261-270
• /
• 2015

Characteristics of snowfall and snow crystal habits have been investigated in the campaign of Experiment on Snow Storms At Yeongdong (ESSAY) using radiosonde soundings, Global Navigation Satellite System (GNSS), and a digital camera with a magnifier for taking a photograph of snowfall crystals. The analysis period is 6 to 14 February 2014, when the accumulated snowfall amount is 192.8 cm with the longest snowfall duration of 9 days. The synoptic situations are similar to those of the previous studies such as the Low pressure system passing by the far South of the Korean peninsula along with the Siberian High extending to northern Japan, which eventually results in the northeasterly or easterly flows and the long-lasting snowfall episodes in the Yeongdong region. In general, the ice clouds tended to exist below around 2~3 km with the consistent easterly flows, and the winds shifted to northerly~northwesterly above the clouds layer. The snow crystal habits observed in the ESSAY campaign were mainly dendrite, consisting of 70% of the entire habits. The rimed habits were frequently captured when two-layered clouds were observed, probably through the process of freezing of super-cooled droplets on the ice particles. The homogeneous habit such as dendrite was shown in case of shallow clouds with its thickness of below 500 m whereas various habits were captured such as dendrites, rimed dendrites, aggregates of dendrites, plates, rimed plates, etc in the thick cloud with its thickness greater than 1.5 km. The dendrites appeared to be dominant in the condition of cloud top temperature specifically ranging $-12{\sim}-16^{\circ}C$. However, the association of snow crystal habits with temperature and super-saturation in the cloud could not be examined in the current study. Better understandings of characteristics of snow crystal habits would contribute to preventing breakdown accidents such as a greenhouse destruction and collapse of a temporary building due to heavy snowfall, and traffic accidents due to snow-slippery road condition, providing a higher-level weather information of snow quality for skiers participating in the winter sports, and estimating more accurate snowfall amount, location, and duration with the fallspeed of solid precipitation.

• 한국석유지질학회지
• /
• 제4권1_2호
• /
• pp.20-26
• /
• 1996

백악기 능주분지를포함한 옥천대 서부 일부지역의 지하 지질구조를 파악하기 위하여 이 지역의 항공자력 자료를 분석 및 해석하였다. 연구지역은 전라남도 무안군, 영암군, 강진군, 장흥군 및 동부 해남군 등이 포함된다. 극자료화 자력이상을 정성적 분석 및 정량적 모델링에 의해 다음과 같은 사실을 알게 되었다. :연구지역의 북서부의 나주군 동강면에서는 대자율이 작은 선캠브리아기의 편마암과 백악기의 불국사 화강암이 분포되어 있다. 그러나 자력이상을 설명하기 위해서는 이들 암석이 지하 깊숙히에서 대자율이 큰 반암에 의해 관입되어 있는 상태를 가정해야만 한다 능주퇴적분지를 동서로 가르는 단면 자력 자료를 모델링한 결과에 의하면 분지의 북부는 남부보다 그 깊이가 더 깊고 최고 깊이가 $3\cal{km}$ 정도 된다. 분지의 기저부는 서부쪽이 동부쪽보다 경사가 더 급하다. 능주분지의 퇴적암의 대자율이 크게 나타나는 점으로 미루어보아 상당한 양의 화산 암류가 포함되어 있을 것으로 유추된다. 이러한 사실은 능주분지에 석유 또는 천연가스의 부존 가능성을 배제한다.

• Toxicological Research
• /
• 제31권4호
• /
• pp.403-414
• /
• 2015

Lithospermum erythrorhizon has long been used as a traditional oriental medicine. In this study, the acute and 28-day subacute oral dose toxicity studies of hexane extracts of the roots of L. erythrorhizon (LEH) were performed in Sprague-Dawley rats. In the acute toxicity study, LEH was administered once orally to 5 male and 5 female rats at dose levels of 500, 1,000, and 2,000 mg/kg. Mortality, clinical signs, and body weight changes were monitored for 14 days. Salivation, soft stool, soiled perineal region, compound-colored stool, chromaturia and a decrease in body weight were observed in the extract-treated groups, and no deaths occurred during the study. Therefore, the approximate lethal dose (ALD) of LEH in male and female rats was higher than 2,000 mg/kg. In the subacute toxicity study, LEH was administered orally to male and female rats for 28 days at dose levels of 25, 100, and 400 mg/kg/day. There was no LEH-related toxic effect in the body weight, food consumption, ophthalmology, hematology, clinical chemistry and organ weights. Compound-colored (black) stool, chromaturia and increased protein, ketone bodies, bilirubin and occult blood in urine were observed in the male and female rats treated with the test substance. In addition, the necropsy revealed dark red discoloration of the kidneys, and the histopathological examination showed presence of red brown pigment or increased hyaline droplets in the renal tubules of the renal cortex. However, there were no test substance-related toxic effects in the hematology and clinical chemistry, and no morphological changes were observed in the histopathological examination of the kidneys. Therefore, it was determined that there was no significant toxicity because the changes observed were caused by the intrinsic color of the test substance. These results suggest that the no-observed-adverse-effect Level (NOAEL) of LEH is greater than 400 mg/kg/day in both sexes.

• 자원환경지질
• /
• 제53권2호
• /
• pp.147-157
• /
• 2020

남극 빙상의 질량 변화는 지구온난화와 관련된 기후 변화와 해수면 상승의 가장 중요한 지표 중 하나이다. 본 연구에서는 위성 중력 자료와 광역 기후 수치 모델을 사용하여, 남극 빙상 질량에 대한 시공간 변화의 특성을 분석하였다. 중력 자료의 분석을 통해 연구 기간(2002.08-2016.08) 동안 지속적으로 남극 빙상의 심각한 질량 손실이 서남극을 중심으로 발생하였음을 확인하였고, 상대적으로 미약한 질량 증가가 동남극에 존재함을 확인하였다. 또한 이들 질량 변동이 해안 지역에 집중되어 있음을 함께 확인하였다. 광역 기후 수치 모델을 사용하여 이러한 질량 변동의 시간적, 공간적 패턴을 유사하게 재현할 수 있었으나, 관측 값에 비해 그 변화 폭이 매우 작았다. 이러한 문제는 빙하의 기저 유출량에 대한 조정을 통해 상당 부분 해결이 되었다. 이 과정에서 재현된 빙상의 질량 변화는 2009년 이전 관측 값의 추세를 97%정도 설명할 수 있었다. 이러한 결과는 빙하 흐름의 역학적 변동이 빙상의 가장 자리를 따라 크게 변하였고, 이러한 변화가 지난 10여 년 동안 남극 빙상의 질량 변화에 크게 영향을 주었다는 것을 의미한다.

• Journal of Microbiology and Biotechnology
• /
• 제19권3호
• /
• pp.331-337
• /
• 2009

Interleukin-13 (IL-13) has been proposed as a therapeutic target for bronchial asthma as it plays crucial roles in the pathogenesis of the disease. We developed an in vitro test system measuring transcriptional downregulatory activities on IL-13 as a primary screening method to select drug candidates from natural products. The promoter region of IL-13 (-2,048 to +1) was cloned into the upstream of a luciferase gene in the plasmid pGL4.14 containing the hygromycin resistance gene as a selection marker, generating pGL4.14-IL-13. The EL-4 thymoma and RBL-2H3 mast cells transiently expressing this plasmid highly produced the luciferase activities by responding to PI (PMA and ionomycin) stimulation up to 8-fold and 13-fold compared with the control, respectively, whereas cyclosporin A, a well-known antiasthmatic agent, significantly downregulated the activities. The BF1 clone of RBL-2H3 cells constitutively expressing pGL4.14-IL-13 was established by selecting surviving cells under a constant lethal dose of hygromycin treatment. The feasibility of this system was evaluated by measuring the downregulatory activities of 354 natural products on the IL-13 promoter using the BF1 clone. An extract from Morus bombycis (named TBRC 156) significantly inhibited PI-induced luciferase activities and IL-13 mRNA expression, but not the protein expression. Fisetin (named TBRC 353) inhibited not only PI-induced luciferase activities and mRNA expression, but also the IL-13 protein secretion, whereas myricetin (named TBRC 354) could not suppress the IL-13 expression at all. Our data indicated that this in vitro test system is able to discriminate the effects on IL-13 expression, and furthermore, that it might be suitable as a simple and time-saving primary screening system to select antiasthmatic agents by measuring transcriptional activities of the IL-13 promoter.