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Molecular Detection and Characterization of Orf Virus from Outbreak of Contagious Pustular Dermatitis in Korean Indigenous Goats  

Park, Jin-Ho (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Guk-Jong (Kim's Animal Clinic)
Choi, Wook (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Kim, Eun-Ha (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Han, Jae-Chul (Livestock Development and Research Institute)
Ou, Sung-Guk (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Lee, Jon-Hwa (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Cho, Mae-Rim (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Song, Hee-Jong (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Chae, Jun-Seok (College of Veterinary Medicine and Bio-Safety Research Institute, Chonbuk National University)
Publication Information
Journal of Veterinary Clinics / v.21, no.2, 2004 , pp. 102-108 More about this Journal
Abstract
Orf virus (ORFV), a member of genus Parapoxvirus (family-Poxviridae), a causative agent of contagious ecthyma in sheep and goat leading to a condition commonly known as vesicular dermatitis. Recently, twelve goats from Iksan in Jeonbuk province were observed with clinical signs like necrotic vesicular lesions around the mucosa of mouth, nasal cavity, eye, ear, teats, abdomen and groin. Based on these clinical symptoms, contagious ecthyma infection was suspected. The skin scrapping was collected from lesions for isolation of DNA and subsequent PCR amplification of ORFV specific 235 bp region of B2L gene. All of the samples were found positive by PCR analysis. Sequencing and further phylogenetic analysis of the PCR product revealed 100% identity to Japan isolate of ORFV (Okinawa, GenBank accession number AB080769), and showed 99.6% of similarity to New Zealand strain (NZ-2, GenBank accession number U06671). It was concluded that ORFV strain detected in the present study is homologous to Japan isolate and New Zealand strain. The PCR test based on amplification of B2L gene is a highly useful tools for rapid and specific diagnosis of contagious ecthyma.
Keywords
Orf virus; contagious ecthyma; B2L gene; PCR amplification; phylogenetic tree;
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