• 한국육종학회지
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• 제40권3호
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• pp.211-214
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• 2008

Haploid system by anther culture allows the development of homozygous lines when doubled. The response of anther culture to Basta (glufosinate) resistance was investigated on transgenic plants (cv. Anjungbyeo) in order to identify inheritance of bar gene associated with Basta. Most of the regenerated transgenic plants were sterile, and only a few plants produced viable seeds ($A_1$) in the greenhouse. The bar gene was analysis by PCR in basta resistant transgenic plant ($TA_0$). The transgenic seeds ($A_1$) were significantly germinated in Basta solution compared with non-transformed seeds. As a result of anther culture, in regenerated haploid plants, segregation ratio was 1:1 in five of eight cross combinations. In diploid plants, segregation ratio was 1:1 in seven of eight cross combinations. Although there was some differences in the cross combinations, most of the combinations had 1:1 segregation ratio which supports the theory. The difference may be a result of the small sample size or the difference of anther culture response caused by genotypic difference. Hence, when many cross combinations were anther-cultured the results would support the theory.

• 분석과학
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• 제37권1호
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• pp.12-24
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• 2024

In this study, laser-induced breakdown spectroscopy (LIBS) was used to estimate the degree of rice polishing. As-threshed rice seeds were dehusked and polished for different times, and the resulting grains were analyzed using LIBS. Various atomic, ionic, and molecular emissions were identified in the LIBS spectra. Their correlation with the amount of polished-off matter was investigated. Na I and Rb I emission line intensities showed linear sensitivity in the widest range of polished-off-matter amount. Thus, univariate models based on those lines were developed to predict the weight percent of polished-off matter and showed 3-5 % accuracy performances. Partial least squares-regression (PLS-R) was also applied to develop a multivariate model using Si I, Mg I, Ca I, Na I, K I, and Rb I emission lines. It outperformed the univariate models in prediction accuracy (2 %). Our results suggest that LIBS can be a reliable tool for authenticating the degree of rice polishing, which is closed related to nutrition, shelf life, appearance, and commercial value of rice products.

• Journal of Biosystems Engineering
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• 제22권4호
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• pp.401-410
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• 1997

Scattering of seeds is one of the problems in the hill- dropping planters with a roller-type metering device. For crops like rice, corns and beans, the hill-dropping is own better for a high yield than the drill planting. The roller type metering device has been used for the drill planters and has many advantages. However, it has a problem of scattering of seeds when they are placed on rows. In this study a method was developed to design the roller type metering device fir the precision hill-droppings. Design parameters were derived to configure completely the geometries of the roller and brush. The method was applied to the design of the metering device of the rice seeding machines. The field test showed that the metering device designed by the method made a better performance of seed placement than that made by the precvious one. The method also can be applied to the design of metering devices for the precise hill-droppings of other crops.

• 한국작물학회지
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• 제42권4호
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• pp.373-377
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• 1997

벼 담수 직파재배와 콩 포장에서 조류 기피제인 methiocarb 50％ WP의 처리에 의한 새 피해 경감 효과를 조사하기 위하여 화성벼와 태광콩을 공시하고 약제의 양을 달리하여 시험한 결과를 요약하면 다음과 같다. 1. <실험 1> 벼 종자에 대한 methiocarb 처리에 따른 새 피해 경감 효과와 모의 생육 1) 벼 담수 직파재배에서 무처리구는 파종 후 2일에 12％, 3일 후에 37％, 5일 후에 50％의 새 피해가 발생하였으나, 벼 종자 5g 처리구는 4일 후에 35％, 5일 후에 39％의 피해가 있었고, 10, 15g 처리구는 파종 후 6일에 9％ 정도이었다. 2) 벼 담수 직파재배에서는 파종 7일 후에는 초엽과 침엽이 출현함으로써 새에 의한 피해가 상당히 감소되는 것으로 나타나서 새에 의한 피해방지는 파종 후 7일 이내가 중요하였다. 3) 약제 처리에 대한 벼 종자의 출아율은 종자 kg 당 methiocard 10g 처리구까지는 차이가 없었으나 15g 처리구에서는 유의하게 출아율이 저하하는 것으로 나타났다. 4) 파종 20일 후의 초장과 엽수는 종자 kg당 methiocarb 15g 처리구가 유의하게 작아지는 것으로 나타났다. 2. <실험 2> 콩에 대한 methiocarb 처리에 따른 새 피해 경감 효과 1) 콩 포장에서 새 피해 발생 정도를 경시적으로 조사한 결과 methiocarb 7.5g 처리구는 무처리구에 비하여 현저하게 낮아지는 것으로 나타났다. 2) 콩 포장에 대한 methiocarb 처리에 의한 약해 정도를 경시적으로 조사한 결과 전 처리구에서 약해 발생은 관찰되지 않았다.

• 한국작물학회지
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• 제53권4호
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• pp.417-420
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• 2008

2006년도에 생산한 일품벼 종자의 키다리병 이병종자와 건전종자를 사용하여 소독 방법별(관행-프로크로라츠 2,000배액 상온 24시간 침지후 침종 5일, 발아상-프로크로라츠 2,000배액 $33^{\circ}C$에서 48시간 침지 및 침종, 무처리-상온 6일 침종) 생육 특성과 수량 등을 분석한 결과는 다음과 같다. 키다리병 이병 종자의 소독 방법별 키다리병 발생율은 파종 후 10일째 무처리와 관행은 각각 76.0%, 5.0%였으나 발아 상 $33^{\circ}C$에서 프로크로라츠 2,000배액에 48시간 동안 소독 시는 발생하지 않았으며, 수확기 때는 발아상 소독시 발병율 3.3%로 관행 9.2%에 비해 낮아 효과적이었다. 수수는 발아상 소독시 30개/주였으나 관행이나 무처리시는 각각 27개, 24개로 적었다. 등숙율은 발아상 소독시 $81.3{\sim}80.3%$로서 관행 75.4%에 비해 $5.9{\sim}4.9%$ 향상 되었고 수량은 $512{\sim}517\;kg/1,000\;m^2$로 관행에 비해 $6{\sim}7%$ 증수되었다. 키다리병 이병 종자라도 발아상 이용 소독시 키다리병은 건전 종자와 비슷하게 발생하여 매우 효과적인 방법이라 할 수 있겠다.

• 현장농수산연구지
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• 제13권1호
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• pp.19-26
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• 2011

최근 우리나라에서는 육묘에서 이앙작업 단축을 위하여 본답육묘(못자리)에서 하우스 육묘(육묘공장) 시스템을 전국적으로 채택하고 있으나 이앙당시 유묘의 연약한 생장으로 모내기 약 2주전 별도로 본답 또는 외기로 육묘상자를 옮겨 경화를 시키는 번거로운 문제점에 있다. 따라서 본 시험에서는 철분코팅볍씨를 이용할 경우 다음과 같은 결과를 얻어 육묘공장(못자리)에서도 본 연구결과를 활용할 경우 유용할 것으로 사료되어진다. 1. 철분코팅을 한 볍씨는 무처리에 비하여 발아, 출아, 유묘생장에 극히 정상적이었다. 2. 이앙당시 유묘의 초장 생장은 무처리에 비하여 작아 육묘상자 운반과정, 이앙작업 시 손상이 줄어 들 것으로 보였다. 3. 유묘의 엽수 뿌리 생육이 무처리에 비하여 양호하여 초기 유묘생장에 유리할 것으로 보였다. 4. 철분코팅을 한 유묘의 지상부, 지하부 생장이 무처리에 비하여 양호하여 건묘육성에 유리한 것으로 나타났다.

• The Plant Pathology Journal
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• 제16권4호
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• pp.236-241
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• 2000

A selective agar medium was developed and tested for the isolation of Acidovorax avenae subsp. avenae, the causal bacterial pathogen of bacterial brown stripe, from rice seeds. The new selective agar medium, designated sorbitol pyroglutamic acid agar (SPA) medium, contained 0.5 g of $K_2$HPO$_4$, 3.0 g of Na$_2$HPO$_4$, 2.0 g of D-sorbitol, 0.2 g of L-pyroglutamic acid, 10.0 $m\ell$ of tween 80, 40.0 mg of victoria blue B, 15.0 g of agar, 150.0 mg of ampicillin and 25.0 mg of vancomycin per litter. Colonies of A. avenae subsp. avenae on SPA medium were smooth, round, convex, shiny, blue and 1.5-2.0 mm in diameter 4 days after incubation at 28$^{\circ}C$. Blue colored colony having dark blue zone was typical type of A. avenae subsp. avenae colonies on the medium. Mean recovery of 8 isolates of A. avenae subsp. avenae on the selective SPA medium was 95.8% in comparison to that on KB medium. The saprophytic bacteria were reduced to 97.9% on SPA medium compared to those on KB medium. Most of other rice seedborne bacteria as well as reported pathogenic bacteria were failed to grow on SPA medium. This medium was highly selective for recovering A. avenae subsp. avenae from rice seed samples, and it could be used to enhance the recovery of this bacterium from rice seed samples, which may be contaminated with large numbers of competing microorganisms.

• 한국작물학회지
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• 제31권3호
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• pp.311-317
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• 1986

수도의 재배 환경차이에 따른 탈립성 변이를 조사한 결과는 다음과 같다. 1. 출수후 일수에 따른 탈립율 변이는 품종간에 다소 상이한 경향이었으나 역탈립성 품종에서 수확시기별 탈발률 변이가 큰 편이었다. 2. 수확후 건조일수에 따른 탈립률 변리는 수확직후보다는 6 일간 건조시켰을 때 탈립률이 훨씬 높게 나타났다. 3. 재배시기별 탈립률 변리는 재배시기가 늦어질수록 탈립률이 낮아졌으며 이러한 경향은 역탈립성품종에서 더욱 뚜렷하게 나타났다. 4. 탈립성의 연차간 변이도 유의한 변이가 인정되었으며 역탈립성 품종에서 더욱 높게 나타났다. 5. 질소 시비수준 증가함에 따라 대체로 탈립률이 높아지는 경향이었으며 역탈립성 품종에서 그 변이정도가 컸다. 6. 출수전 11∼5일 사이의 저온처리와 저온 및 차광처리에서 탈립률이 가장 높게 나타났으며 밀양2003는 저온처리에 비해 저온과 차광을 동시에 처리했을 때 탈립률이 더욱 높게 나타났다.

• 한국육종학회지
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• 제42권2호
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• pp.144-150
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• 2010

The objective of this study was to map gene/QTL for photoblastism in a weedy rice (photoblastic rice: PBR) using DNA markers. Light-induced effect on germination of seeds was compared among three accessions (Oryza sativa L.), PBR, Milyang 23 and Ilpum. Results showed that PBR seeds started to show photoblastism during seed development, different from Ilpum and Milyang 23. Frequency distribution of germination in the F4 lines from crosses between Ilpum and PBR and, Milyang 23 and PBR revealed bimodal distributions suggesting that photoblastism was controlled by a few genes. Bulked segregant analysis using $F_4$ populations derived from the above two crosses was conducted to identify gene/QTL for photoblastism. Two QTL were identified on chromosomes 1 and 12 explaining 11.2 and 12.8% of the phenotypic variance, respectively. Two QTL were further mapped between two SSR markers, RM8260 and RM246 on chromosome 1, and between RM270 and 1103 on chromosome 12. It is noteworthy that two QTL for photoblastism were colocalized with the QTL for seed dormancy reported in the previous QTL studies. The clustering of two genes for photoblastism and dormancy possibly indicates that these regions constitute rice phytochrome gene clusters related to germination. Because PBR has a low degree of dormancy, a pleiotropic effect of a single gene controlling dormancy and photoblastism can be ruled out. The linked markers will provide the foundation for positional cloning of the gene.

• 한국농업기계학회:학술대회논문집
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• 한국농업기계학회 1996년도 International Conference on Agricultural Machinery Engineering Proceedings
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• pp.1009-1020
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• 1996

Direct-seeded rice can have comparable yield with transplanted rice if its inherent problems can be solved. It is a labor-saving technology and can significantly reduce production cost because seedling nursery , pulling , and transplanting are omitted. Turnaround time between cropping is reduced hence the possibility of a third annual crop. But direct-seeded rice is very vulnerable to pest attack (by birds, rats, and golden snails), desiccation, weed infestation, and prone to lodging resulting to unstable crop establishment and inconsistent yield. These problems can be solved by anaerobic seeding (sowing pre-germinated seeds under the soil). It requires precise seed placement into the soil to optimize its benefits. We developed a four-row anaerobic direct seeder (US $ 200 commercial price) for this purpose . It consist of a structural framework mounted with a drum -hopper metering device, flotation type drivewheels, spring-loaded and adjustable furrow closers, and furrow open rs, and a plastic rainguard. It can sow in line pre-germinated seeds into the soil thus permitting the use of mechanical weeders for a chemical-free weed control. Its performance was comparable with the Japanese two-row anaerobic seeder (costing US$400) in terms of seed placement and crop establishment. It was tested with five cultivars. Seeding rate varied from 38 kg/ha to 80kg/ha. Crop establishment ranged from 64 to 99 percent while grain yield varied from 3.0 t/ha to 5.4t/ha. A six-row anaerobic seeder was also developed and adapted to a powertiller for increased capacity , field efficiency , and easier operation. The anaerobic seeder is useful to farmers shifting to direct seeding to reduce rice production cost and to researchers conducting agronomic studies in direct-seeded rice. Blueprint of the machine is available free of charge from IRRI.