• 대한영양사협회학술지
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• 제26권1호
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• pp.1-13
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• 2020

This study was undertaken to optimize preparation of sweet rice muffins using lotus (Nelumbo nucifera Gaertn) seed powder. The experimental conditions used in the preparation included lotus seed powder (X₁) and soybean oil (X₂). The muffin formulation was optimized using rheology. Lightness (P<0.01) displayed a quadratic model pattern, whereas yellowness (P<0.01) and redness (P<0.05) was presented a linear model. Texture properties significantly differed in cohesiveness (P<0.05). Evaluating the sensory characteristics of muffins, except for flavor and texture, the remaining properties of color (P<0.01), taste, overall quality, and appearance (P<0.05) were found to be significantly different. The overall quality was also affected by the proportion of lotus seed powder, rather than the amount of soybean oil. The optimal ratio for palatability of muffins was determined to be 47.80 g lotus seed powder and 95.28 g soybean oil. Taken together, our results indicate that sweet rice muffins prepared using lotus seed powder are sufficiently competitive in terms of function and quality.

• 농약과학회지
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• 제7권3호
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• pp.216-222
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• 2003

벼 육묘기간 중에 발생되는 벼 키다리병의 방제법을 확립하고자 최근에 육성된 벼 품종별 발병 정도와 시판되는 종자소독제의 방제효과를 조사하였으며 종자소독시 약액의 온도, 처리시간 및 약량에 따른 벼 키다리병 방제효과를 조사하였다. 품종별 벼 키다리병 발생정도를 조사한 결과 화영벼, 동진벼, 호안벼, 농호벼, 남평벼, 호진벼 6 품종이 저항성 품종으로, 그루벼, 소비벼, 오대벼, 주남벼, 삼천벼, 상주벼, 화봉벼 7 품종이 감수성 품종으로 분류되었다. 종자소독제별 벼 키다리병 방제효과에 있어서 prochloraz, fludioxonil, carpropamid+imidacloprid+fludioxonil 등은 높은 방제 효과를 보였으나 benomyl+thiram, thiophanatemethy+thiram, thiophanate-metyl+triflumizole등은 방제효과가 저조하였다. 종자소독당시 수온에 따른 벼 키다리병 방제가는 $10^{\circ}C$에서는 61% 이었으나 온도가 높아질수록 상승하여 $30\sim35^{\circ}C$에서는 벼 키다리병을 $95\sim98%$이상 방제할 수 있었다. 그러나 종자소독시간과 약량은 벼 키다리병 방제에 크게 영향을 미치지 않았다. 따라서 벼 키다리병을 효율적으로 방제하기 위해서는 저항성이 강한 벼 품종과 효과가 우수한 종자 소독제 선정 그리고 종자소독시 적정수온을 유지하는 것이 중요한 것으로 나타났다.

• 한국작물학회지
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• 제39권3호
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• pp.270-277
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• 1994

본 연구는 소백벼, 중원벼 등 국내 재배품종과 교배모본으로 이용되는 재래 및 도입품종을 공시하여 저장조건에 따른 종실의 휴면성의 변화를 알고자 수행 되었으며 결과를 요약하면 아래와 같다. 1. 출수후 40일 된 종실의 수확직후 및 저장기간에 따른 발아율을 조사한 결과 자포니카인 화성벼와 상풍벼, 샤레형 재래종인 자광도와 몽근샤레, 그리고 통일형 품종인 삼강벼는 성숙종자에 휴면성이 거의 없었고, 자포니카형 조생종인 소백벼와 오대벼는 약한 휴면성을, 그리고 통일형인 중원벼와 인디카형 품종인 IR-20과 IR-42는 강한 휴면성을 가지고 있었다. 2. 출수후 40일에 수확한 종실은 2$0^{\circ}C$ 항온 또는 서야 25/15$^{\circ}C$ 변온조건에 30일동안만 저장하여도 휴면성이 강한 품종을 포함한 모든 공시품종이 90%이상의 높은 발아율을 보였으나, 4$^{\circ}C$, 상대습도 40%조건에서 30일에서 180일까지 저장했을 때는 품종간 발아율 차이가 인정되었고, 휴면성이 강한 인디카형 품종인 IR-20은 180일간 저장후에도 발아율이 60%에 미치지 못하였다. 3. 성숙한 종실을 2$0^{\circ}C$ 또는 서야 20/15$^{\circ}C$ 조건에 30일 이상 180일까지 저장하면 모든 품종이 수확직후의 종실보다 발아기까지의 일수가 짧아졌으며, 4$^{\circ}C$에 저장했을때도 고온조건에서보다는 그 정도가 낮지만 저장기간이 길어질수록 발아율이 높아지고 발아일수도 빨라졌다.

• 한국작물학회지
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• 제53권4호
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• pp.434-439
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• 2008

The amount of salt to make seed sorting solution of the specific gravity of 1.13 was reconsidered and determined as 3.8 kg salt in 18 L water, which is lower amount than currently used. Five rice cultivars were examined. Percent germination and seedling emergence were not similar. Seedling emergence rate of Japonica varieties, Nampyungbyeo and Daerypbyeo-1 were 87% and 95% under specific gravity of 1.13, respectively. Seedling emergence rate of Tongil type variety, Dasanbyeo was as high as 67% in specific gravity of 1.06. Seedling emergence rate of waxy rice, Hwasunchalbyeo and Aranghangchalbyeo were examined. Seedling emergence rate was 94% in both cultivars in specific gravity of 1.04. Seedling emergence rate was same in specific gravity of 1.08 which is generally used for selecting seed currently. Early growth (plant height, leaf number, and dry weight) were not significantly different by specific gravity within species. In all cultivars except waxy rice, highest seedling emergence rate was observed in specific gravity of 1.13 which is currently used for selection and decreased as specific gravity is lowed. However, considering total amount of seeds in each group of specific gravity, amount of seed in lower specific gravity group is relatively small and total seedling emergence rate within variety dose not show big difference. However, if seeds with low speicific gravity are produced due to the bad grain filling condition and consequently total seed content of low specific gravity increases, results will be differnt. Reduction in total growth and yield could occur. It will be important to comply with the seed sorting criterion of 1.13 for Japonica, 1.06 for Tongil, and 1.04 for waxy rice variety to ensure the maximum rice growth and yield.

• The Plant Pathology Journal
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• 제34권6호
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• pp.490-498
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• 2018

Panicle blight and seed rot disease caused mainly by Burkholderia glumae and Burkholderia gladioli is threatening rice cultivation worldwide. The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent cross-reactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and $3.86{\times}10^2cells$ for B. glumae and $5.85{\times}10^2cells$ for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with a detection limit as low as $1.86{\times}10^3cells$ per rice seed for B. glumae and $1.04{\times}10^4cells$ per rice seed of B. gladioli. The novel primers maybe valuable as a more sensitive, specific, and robust tool for the efficient simultaneous detection of B. glumae and B. gladioli on rice seeds, which is important in combating rice panicle blight and seed rot by early detection and confirmation of the dissemination of pathogen-free rice seeds.

• 한국산업정보학회논문지
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• 제25권5호
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• pp.23-29
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• 2020

농업진흥청 국립농업과학원에서는 다양한 종류의 농작물에 대해 우량 종자 확보를 위한 생육환경 모니터링 및 수확된 종자의 분석과 같은 다양한 연구를 진행하고 있다. 본 논문에서는 농업진흥청에서 보유하고 있는 다양한 종류의 농작물 씨앗을 분석하기 위해 종자 객체 검출 방법을 제안한다. 제안된 방법은 Mask-RCNN을 이용한 전이학습을 수행하며 주어진 특정 환경 (일정한 조도, 흰색 배경)에서 촬영한 입력 영상을 종자 객체 인식을 위한 적절한 매개 변수 적합 (Tuning) 과정 및 영상 분할 작업을 진행한다. 제안된 방법으로 종자 객체 검출에 대한 실험결과로 벼 이삭 영상의 경우 82%와 단순한 볍씨 영상의 경우 97%의 정확도로 벼 낱알을 검출하였다. 향후 연구로 복잡한 상황의 종자 영상 분할을 위한 심화학습 기반의 접근법 및 검출된 종자 객체로부터 길이, 폭, 두께와 같은 정밀한 데이터 분석을 통하여 우량 종자 연구를 계획하고 있다.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2019년도 추계학술대회
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• pp.205-205
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• 2019

• 한국응용곤충학회지
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• 제23권2호
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• pp.126-131
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• 1984

1. 영남작물시험장, 호남작물시험장 및 충남농촌진흥원에서 분양받은 18개의 벼종자시료와 충남지역 일반농가에서 수확한 8개의 벼종자시료 등 모두 26개의 종자시료를 공시하여 표준습지법으로 조사하였던 바 21종의 균류가 검출되었으며, 벼 갈샐잎마름병균(Gerlachia oryzae)은 22개의 벼 종자시료에서 $1.0\~45.0\%$의 범위로 검출되었다. 2. 종자전염성 G. oryzae의 효과적인 검출방법을 찾기 위하여 표준습지법, 냉동습지법, 물한천법 등으로 비교 조사한 결과, 냉동습지법에서 검출율이 가장 높았다. 3. 벼종자의 각 부위를 무균적으로 분리하여 부위별로 G. oryzae의 감염여부를 표준습지법으로 조사한 결과, 벼 종자의 껍질에서 가장 많이 검출되었으며, 심하게 이병된 종자는 배유 및 종피 뿐 만 아니라 배에 까지 감염되어 있었다. 4. G. oryzae에 감염된 벼 종자를 파종하여 심하게 이병된 종자는 종자부패 및 묘입고를 일으켰으며, 약하게 이병된 종자는 초엽, 1엽, 2엽에 갈색의 병징감염을 초래하였다.

• Journal of Plant Biotechnology
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• 제39권3호
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• 현장농수산연구지
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• 제21권2호
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• pp.77-86
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• 2019

To determine none top soil covering in rice seedling nursery method for the sparse machine transplanting, four different sowing methods were tested. Shoot and root length, fresh weight, leaf number and color using leaf color chart(LCC) and SPAD were collected as the data comparison of methods. The seedling height showed the highest growth according to the conventional (230g seed rate of pre-emerged seeds and top-soil covering) > high sowing density 1 (290g seed rate of pre-emerged seeds and top-soil covering) ≥ high sowing density 2(290g seed rate of pre-emerged seeds and none top-soil covering) > high sowing density 3(290g seed rate of iron-coated seeds and none top-soil covering). There was any statistical difference between groups in root length, leaf number, LCC, and SPAD values. Thus, a high sowing density of 290g for rice nursery seedling box was recommended to the sparse machine transplanting in rice cultivation with the none top-soil covering method, enabling convenient handling in transportation and machine transplanting work.