• Journal of the Korean Dietetic Association
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• v.26 no.1
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• pp.1-13
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• 2020

This study was undertaken to optimize preparation of sweet rice muffins using lotus (Nelumbo nucifera Gaertn) seed powder. The experimental conditions used in the preparation included lotus seed powder (X₁) and soybean oil (X₂). The muffin formulation was optimized using rheology. Lightness (P<0.01) displayed a quadratic model pattern, whereas yellowness (P<0.01) and redness (P<0.05) was presented a linear model. Texture properties significantly differed in cohesiveness (P<0.05). Evaluating the sensory characteristics of muffins, except for flavor and texture, the remaining properties of color (P<0.01), taste, overall quality, and appearance (P<0.05) were found to be significantly different. The overall quality was also affected by the proportion of lotus seed powder, rather than the amount of soybean oil. The optimal ratio for palatability of muffins was determined to be 47.80 g lotus seed powder and 95.28 g soybean oil. Taken together, our results indicate that sweet rice muffins prepared using lotus seed powder are sufficiently competitive in terms of function and quality.

• The Korean Journal of Pesticide Science
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• v.7 no.3
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• pp.216-222
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• 2003

In order to develop effective control methods for Bakanae disease caused by Gibberella fujikuroi (Saito) Ito during rearing of rice seedlings, we investigated the disease resistance of 15 rice varieties to G. fujikuroi and control effect of six seed disinfectants, and tried to improve the using methods of the seed disinfectants. Disease resistance was tested by investigating the disease incidence on each rice cultivar grown in rice seedling box infested with or not infested with G. fujikuroi at 30 days after sowing seeds and 20 days after heading date. The results showed that Hwayongbyeo, Dongjinbyeo, Hwoanbyeo, Nonghobyeo, Nampyeongbyeo, and Hwojinbyeo were resistant G. fujikuroi, meanwhile Keulubyeo, Sobibyeo, Odaebyeo, Junambyeo, Samchonebyeo, Sangjubyeo, and Hwabongbyeo were susceptible. Three seed disinfectants, prochloraz, fludioxonil, and carproamid + thiram + fludioxonil controlled Bakanae disease of rice very well, while bonomyl + thiram, thiophanate-methyl + thiram and thiophanate-methyl + triflumizole did not suppress the disease enough. Water temperature was turned to be an important factor for controlling the disease by treating seed disinfectants. Prochloraz showed 61% control value on the disease at $10^{\circ}C$, but it showed above 95% control value at the range of $30-35^{\circ}C$. It was confirmed that the control effect of seed disinfectants increased with increasing water temperature. Meanwhile soaking period of rice seeds in the suspension of seed disinfectants and chemical dosage had no high relation to control the disease. This results suggest that rice varieties, water temperature, and optimal selection of suitable seed disinfectants are very important to control Bakanae disease effectively.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.3
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• pp.270-277
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• 1994

This study was conducted to investigate change of seed dormancy during storage in rice. The result of germination rate observed at different storage duration after harvest indicated that two Japonica rice varieties, Sangpungbyeo and Hwaseongbyeo, tow sare type local varieties, Jakwangdo and Mongeunsare, and a Tongil type rice, Samgangbyeo, showed no dormancy in rice seed harvested at 40 days after heading, However, weak dormancy was found in matured rice seed of Sobaekbyeo and Odaebyeo, and strong seed dormancy was found in Jungwonbyeo, IR-20 and IR-42. Rice seed stored for 30 days after harvest at 2$0^{\circ}C$ constant temperature or 25/15$^{\circ}C$ of day /night temperature fluctuation showed higher germination rate above 90% in all varieties tested. But germination rate of seeds stored at 4$^{\circ}C$ for 30 to 180 days showed varietal difference, higher in rice varieties having weak seed dormancy and lower in strong dormancy varieties. Days to 80% germination and average days for germination were shortened by storage for 30 to 180 days after harvest at 2$0^{\circ}C$, 4$^{\circ}C$ with RH 40% or day /night 25/15$^{\circ}C$ temperature condition in all rice varieties tested, but the degree of acceleration was lower at the condition of 4$^{\circ}C$ with RH 40%.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.4
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• pp.434-439
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• 2008

The amount of salt to make seed sorting solution of the specific gravity of 1.13 was reconsidered and determined as 3.8 kg salt in 18 L water, which is lower amount than currently used. Five rice cultivars were examined. Percent germination and seedling emergence were not similar. Seedling emergence rate of Japonica varieties, Nampyungbyeo and Daerypbyeo-1 were 87% and 95% under specific gravity of 1.13, respectively. Seedling emergence rate of Tongil type variety, Dasanbyeo was as high as 67% in specific gravity of 1.06. Seedling emergence rate of waxy rice, Hwasunchalbyeo and Aranghangchalbyeo were examined. Seedling emergence rate was 94% in both cultivars in specific gravity of 1.04. Seedling emergence rate was same in specific gravity of 1.08 which is generally used for selecting seed currently. Early growth (plant height, leaf number, and dry weight) were not significantly different by specific gravity within species. In all cultivars except waxy rice, highest seedling emergence rate was observed in specific gravity of 1.13 which is currently used for selection and decreased as specific gravity is lowed. However, considering total amount of seeds in each group of specific gravity, amount of seed in lower specific gravity group is relatively small and total seedling emergence rate within variety dose not show big difference. However, if seeds with low speicific gravity are produced due to the bad grain filling condition and consequently total seed content of low specific gravity increases, results will be differnt. Reduction in total growth and yield could occur. It will be important to comply with the seed sorting criterion of 1.13 for Japonica, 1.06 for Tongil, and 1.04 for waxy rice variety to ensure the maximum rice growth and yield.

• The Plant Pathology Journal
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• v.34 no.6
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• pp.490-498
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• 2018

Panicle blight and seed rot disease caused mainly by Burkholderia glumae and Burkholderia gladioli is threatening rice cultivation worldwide. The bacteria have been reported as seed-borne pathogens from rice. Accurate detection of both pathogens on the seeds is very important for limiting the disease dissemination. Novel primer pairs targeting specific molecular markers were developed for the robust detection of B. glumae and B. gladioli. The designed primers were specific in detecting the target species with no apparent cross-reactions with other related Burkholderia species at the expected product size. Both primer pairs displayed a high degree of sensitivity for detection of B. glumae and B. gladioli separately in monoplex PCR or simultaneously in duplex PCR from both extracted gDNA and directly preheated bacterial cell suspensions. Limit of detection was as low as 0.1 ng of gDNA of both species and $3.86{\times}10^2cells$ for B. glumae and $5.85{\times}10^2cells$ for B. gladioli. On inoculated rice seeds, the designed primers could separately or simultaneously detect B. glumae and B. gladioli with a detection limit as low as $1.86{\times}10^3cells$ per rice seed for B. glumae and $1.04{\times}10^4cells$ per rice seed of B. gladioli. The novel primers maybe valuable as a more sensitive, specific, and robust tool for the efficient simultaneous detection of B. glumae and B. gladioli on rice seeds, which is important in combating rice panicle blight and seed rot by early detection and confirmation of the dissemination of pathogen-free rice seeds.

• Journal of Korea Society of Industrial Information Systems
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• v.25 no.5
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• pp.23-29
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• 2020

The National Institute of Agricultural Sciences of the Rural Developement Administration (NAS, RDA) is conducting various studies on various crops, such as monitoring the cultivation environment and analyzing harvested seeds for high-throughput phenotyping. In this paper, we propose a deep learning-based rice seed segmentation method to analyze the seeds of various crops owned by the NAS. Using Mask-RCNN deep learning model, we perform the rice seed segmentation from manually taken images under specific environment (constant lighting, white background) for analyzing the seed characteristics. For this purpose, we perform the parameter tuning process of the Mask-RCNN model. By the proposed method, the results of the test on seed object detection showed that the accuracy was 82% for rice stem image and 97% for rice grain image, respectively. As a future study, we are planning to researches of more reliable seeds extraction from cluttered seed images by a deep learning-based approach and selection of high-throughput phenotype through precise data analysis such as length, width, and thickness from the detected seed objects.

• Proceedings of the Korean Society of Crop Science Conference
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• 2019.09a
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• pp.205-205
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• 2019

• Korean journal of applied entomology
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• v.23 no.2 s.59
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• pp.126-131
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• 1984

Twenty one fungi were detected from 26 rice seed samples obtained from Yeongnam Crop Experiment Station, Honam Crop Experiment Station, Chungnam Provincial Office of Rural Development and farmers of Chungnam Province. Gerlachia oryzae was one of frequently detected fungi with $1.0\~45.0\%$ of detection ratio from 22 rice seed samples. The deep-freezing method was selected as the most suitable for routine seed health testing when the blotter method, deep-freezing method and agar plate method were evaluated for efficiency of detecting seed-borne G. oryzae from rice seeds. Our data obtained by seed component plating technique suggested that G. oryzae was present not only on chaff, in endosperm and in seed coat, but also in the embryo. Seed-borne G. oryzae caused seed rot, seedling blight and symptom of brownish discoloration on coleoptile primary and 2nd leaf when the infected seeds were sown in agar of test tube or in soil.

• Journal of Plant Biotechnology
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• v.39 no.3
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• pp.169-174
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• 2012

Quantitative trait loci (QTLs), which were related to the ability of callus induction and plant regeneration in seed culture of rice, were analyzed using a mapping population from a cross between the rice cultivars 'Samgang' (tongil type) and 'Nagdong' (japonica). A tongil type rice cultivar, 'Samgang' showed lower frequency (20%) of plant regeneration than that (35%) of japonica rice, 'Nagdong'. Transgressive segregations were observed for the ability of callus induction and plant regeneration from the seed-derived calli of 58 doubled haploid (DH) lines. The ability of plant regeneration of 58 doubled haploid lines showed a continuous distribution with comparatively wide range (10.0 to 66.7%) of variation. Composite interval mapping analysis was used to identify the QTLs controlling callus induction and plant regeneration ability. Four significant QTLs, qCWS6, qCWS8, qCWS9 and qCWS11, associated with callus weight per seed were detected on chromosomes 6, 8, 9, and 11 with LOD values of 3.30, 2.60, 2.70 and 2.43, explaining 36% of the total phenotypic variation. Three significant QTLs, qPR1, qPR6, and qPR11, for the ability of plant regeneration were located on chromosome 1, 6, and 11 at LOD score of 2.25, 2.15 and 2.55, accounting for 24 % of the total phenotypic variation. The present study should be useful for improving the efficiency of plant regeneration in tissue culture of indica rice by means of marker-assisted selection.

• Journal of Practical Agriculture & Fisheries Research
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• v.21 no.2
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• pp.77-86
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• 2019

To determine none top soil covering in rice seedling nursery method for the sparse machine transplanting, four different sowing methods were tested. Shoot and root length, fresh weight, leaf number and color using leaf color chart(LCC) and SPAD were collected as the data comparison of methods. The seedling height showed the highest growth according to the conventional (230g seed rate of pre-emerged seeds and top-soil covering) > high sowing density 1 (290g seed rate of pre-emerged seeds and top-soil covering) ≥ high sowing density 2(290g seed rate of pre-emerged seeds and none top-soil covering) > high sowing density 3(290g seed rate of iron-coated seeds and none top-soil covering). There was any statistical difference between groups in root length, leaf number, LCC, and SPAD values. Thus, a high sowing density of 290g for rice nursery seedling box was recommended to the sparse machine transplanting in rice cultivation with the none top-soil covering method, enabling convenient handling in transportation and machine transplanting work.