• The Plant Pathology Journal
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• v.21 no.4
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• pp.402-405
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• 2005

We have previously identified genes for four different protease inhibitors (PIs) that were induced upon rice blast infection in a rice blast resistant mutant SHM-11. Our expression analysis of the PIs indicated that induction of the PIs was the highest 24 hr after rice blast inoculation in the rice mutant SHM-11. Three PIs in the group of serine PIs were highly expressed while a cystein PI was weakly expressed upon rice blast inoculation. Four PIs were weakly induced 48 hr after pathogen inoculation in rice blast susceptible wild type rice plant. The simultaneous expression of three serine PIs was apparent from SHM-11 and two of them were induced in rice blast resistant Taebaegbyeo. One of them was induced in rice blast resistant Hwayeongbyeo while none of them were expressed in rice blast susceptible Nagdongbyeo and rice blast resistant Dongjinbyeo. Our results suggest that the expression of PI gene is rice cultivar specific and may be linked with the rice blast resistance in a specific rice mutant by the simultaneous expression of the PI genes.

• Korean Journal Plant Pathology
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• v.13 no.2
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• pp.79-84
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• 1997

Incidence of rice blast on new rice cultivars and elite lines was observed from 194 to 1996 in Icheon, Chuncheon, Jecheon and Naju areas. The observations were made in the nuseries and in the fields. In the nurseries, only cultivars Daesanbyeo and Hyangmibyeo 2 showed moderate levels of resistance to leaf blast, with the disease index 0 to 6. From the field observations, it was found that cultivars Hyangmibyeo 2 and Suwon 414 were highly resistant to leaf blast, but susceptible to neck blast. the fields, leaf blast was not observed. In general, there was great yearly and regional variation in the incidence of neck blast within the same cultivars, some times ranging from 0 to 100% of incidence. However, the range of fluctuation in the disease incidence were relatively small in the cultivars Daejinbyeo (0∼17.5%), Daesanbyeo (0∼4.0%), Donganbyeo (0∼21.4%) and Hwasambyeo (0∼13.9%). Hyangmibyeo 2 and Seojinbyeo were rarely infected with neck blast in Chuncheon and Naju all of the years, the same cultivars were severely infested with neck blast; 45.1 and 45.5%, respectively, in Jecheon in 1995. The occurrence of different races of rice blast fungus were different at different areas. However, it was found that in Icheon, Chuncheon, Jecheon and Naju areas, the dominant races were KI-409, KJ-201 and KJ-301.

• The Plant Pathology Journal
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• v.38 no.1
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• pp.46-51
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• 2022

Rice blast is the most destructive disease threatening stable rice production in rice-growing areas. Cultivation of disease-resistant rice cultivars is the most effective way to control rice blast disease. However, the rice blast resistance is easy to breakdown within years by blast fungus that continually changes to adapt to new cultivars. Therefore, it is important to continuously monitor the incidence of rice blast disease and race differentiation of rice blast fungus in fields. In 2020, a severe rice blast disease occurred nationwide in Korea. We evaluated the incidence of rice blast disease in Yeoju and compared the weather conditions at the periods of rice blast disease in 2019 and 2020. We investigated the races and avirulence genes of rice blast isolates in Yeoju to identify race diversity and genetic characteristics of the isolates. This study will provide empirical support for rice blast control and the breeding of blast-resistant rice cultivars.

• Journal of Crop Science and Biotechnology
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• v.11 no.3
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• pp.205-214
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• 2008

Blast disease caused by the fungal pathogen, Magnaporthe oryzae, is one of the most damaging diseases in rice. The use of resistant varieties is an effective measure to control the disease, however, many resistant varieties were broken down to their resistance effects by the differentiating of new virulent isolates. This study was done to analyze the haplotypes of 31 microsatellite markers linked to five major R genes and two QTLs and to identify the alleles for the putatively novel genes related to durable resistance to blast in 56 Korean japonica and four indica varieties. The 31 microsatellite markers produced 2 to 13 alleles(mean = 5.4) and had PICi values ranging from 0.065 to 0.860(mean=0.563) among the 60 rice accessions. Cluster analysis based on allele diversities of 31 microsatellite markers grouped into 60 haplotypes and ten major clusters in 0.810 genetic similarity. A subcluster IV-1 grouped of early flowering varieties harboring Piz and/or Pi9(t) on chromosome 6 and Pita/Pita-2 gene on chromosome 12. The other subcluster V-1 consisted of four stable resistance varieties Donghae, Seomjin, Palgong and Milyang20. The analysis of putative QTLs associated with seven blast resistance genes using ANOVA and linear regression showed high significance to blast resistance across regions and isolates in the markers of two genes Piz and/or Pi9(t) and Pita/Pita-2. These results illustrate the utility of microsatellite markers to identify rice varieties is likely carrying the same R genes and QTLs and rice lines with potentially novel resistant gene.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.4
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• pp.329-341
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• 2011

The objective of this study was to determine the genetic diversities of major rice blast resistance genes among 84 accessions of aromatic rice germplasm. Eighty four accessions were characterized by a dominant 11 set of PCR-based SNP and CAPS marker, which showed the broad spectrum resistance and closest linkage to seven major rice blast resistance (R) genes, Pia, Pib, Pii, Pi5 (Pi3), Pita (Pita-2), and Pi9 (t). The allele specific PCR markers assay genotype of SCAR and STS markers was applied to estimate the presence or absence of PCR amplicons detected with a pair of PCR markers. One indica accession, Basmati (IT211194), showed the positive amplicons of five major rice blast resistance genes, Pia, Pi5 (Pi3), Pib, Pi-ta (Pi-ta2), and Pik-5 (Pish). Among 48 accessions of the PCR amplicons detected with yca72 marker, only five accessions were identified to Pia gene on chromosome 11. The Pib gene was estimated with the NSb marker and was detected in 65 of 84 accessions. This study showed that nine of 84 accessions contained the Pii gene and owned Pi5 (Pi3) in 42 of 84 accessions by JJ817 and JJ113-T markers, which is coclosest with Pii on chromosome 9. Only six accessions were detected two alleles of the Pita or Pita-2 genes. Three of accessions were identified as the Pi9 (t) gene locus.

• Korean Journal of Agricultural Science
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• v.16 no.1
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• pp.1-18
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• 1989

This experiment was undertaken to clarify derivation of resistance of Japonica type of rice varieties to rice blast in Korea and to classify Japonica type of rice varieties on the basis of their rice blast reaction in the blast nuisery test. 1. The resistance of Iljin, Kwancheok 9, Koshi, Baedal and Paldal, Jaekeon, Sinpung, Jinheung, Hokwang and Palkeum, and Gwangmyungbyeo and Yeongdeogbyeo to rice blast was derived from Kyudai Taicho Asahi 3, Futaba and 2067, respectively. 2. The resistance of Kwanok, Mankyeong and Nongbaek to the rice blast was derived from Kanto 55, Hokwang and Kusabue, and Ishigarisiroge, respectively. 3. The resistance of Seomjinbyeo, Sinseonchalbyeo, Donghaebyeo and Tamjinbyeo to the rice blast was derived from Milyang 20 and the source of resistance to the rice blast in Jinjubyeo and Daecheongbyeo was HR 769 or HR 1590. 4. The resistance of Dobongbyeo, Gwanagbyeo and Chiagbyeo to the rice blast was derived from Tjina, Kongo and Kuik 90, respectively. 5. The resistance of Seolagbyeo, Seonambyeo, Kihobyeo, Namyangbyeo, Samnambyeo, Seohaebyeo, Whaseongbyeo, Daegwanbyeo and Taeseongbyeo, and Sobaegbyeo, Odaebyeo and Unbongbyeo to the rice blast was derived from Fuji 280 and Fuji 269, respectively. 6. The source of resistance to the rice blast in Cheonmabyeo and Baegambyeo was BL 7 and Nongbaek, the resistance of Dongjinbyeo and Sangpungbyeo to the rice blast was derived from Satominori and Simokita, respectively. 7. Japonica type of rice varieties was classified into eleven varietal groups according to their reaction to the blast as follows. Eight varieties of Jinheung group, two varieties of Dongjinbyeo group, two varieties of Jinjubyeo group, three varieties of Gwanagbyeo group, four varieties of Sobaegbyeo group, one variety of Nongbaek group, two varieties of Baegambyeo group, five varieties of Sinseonchalbyeo group, five varieties of Seonambyeo group, two varieties of Taeseongbyeo group and some variety of Nagdongbyeo group.

• Korean Journal of Agricultural Science
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• v.16 no.2
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• pp.129-137
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• 1989

This experiment was undertaken to clarify derivation of resistance of Tongil type of rice varieties to rice blast in Korea and to classify Tongil type of rice varieties on the basis of their rice blast reactions in th blast nursery test. 1. The resistance of Tongil, Josaengtongil, Yeongnamjosaeng, Hwanggeumbyeo, Honamjosaeng, Noupung, Milyang 21, Milyang 22, Milyang 23, Raekyung, Manseogbyeo, Yongmunbyeo and Yongjubyeo to rice blast was derived from IR 8 or IR 24. 2. The resistance of Milyang 20, Nampungbyeo and Milyang 42, and Samseongbyeo, Seogwangbyeo, Pungsanbyeo and Shingwangbyeo to the rice blast was derived from IR 946 and IR 1539, and IR 1545, respectively. 3. The resistance of Palgwangbyeo, Sujeongbyeo, Hangangchalbyeo, Baegunchalbyeo, Samgangbyeo and Weonpungbyeo, and Taebaegbyeo and Chupungbyeo, and Kayabyeo to the rice blast was derived from IR 2061(IR 29), IR 747 and IR 32, respectively. 4. Cheongcheongbyeo, and Jungweonbyeo and Namyeongbyeo, and Changseongbyeo to the rice blast was derived from IR 2035, IR 5533, and HR 2797 and HR 1671, respectively. 5. Tongil type of rice varieties was classified into Tongil group, Milyang 30 group, Baegyangbyeo group and Taebaegbyeo group.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.67.2-68
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• 2003

A rice cultivar, Taebaegbyeo, is highly resistant to rice blast and moderately resistant to bacterial leaf blight (BLB) caused by Magnaporthe grisea and Xanthomonas oryzae pv. oryzae, respectively. To study the rice disease resistance mechanism, we generated rice deletion M3 mutants by gamma-ray irradiation. Blast and BLB responses of 16,000 M3 mutants were screened by inoculating mixtures of 4 races (KJ-201, H-1113a, KI-313, KI-409) of M. grisea and 3 Korean races of X. oryzae pv. oryzae. We selected so far 21 M3 mutants of Taebaegbyeo showing high susceptibility to the diseases. One of the mutants, KCT-6417, was susceptible to KI-1113a race of M. grisea, suggesting the deletion of a race-specific blast resistance gene in the mutant. To isolate rice genes involved in blast resistance and defense response, we take a PCR-based suppression subtractive hybridization approach using cDNAs of blast-inoculated wild type and the KCT-6417 as a tester and a driver, respectively. Genes specifically expressed in the wild type will be presented. The selected genes would give us a clue to understand mechanism for the race specific resistance and defense responses against M. grisea H-1113a in Taebaegbyeo.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.64.2-64
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• 2003

Twenty-seven monogenic rice lines harboring major resistant gene for blast were screened to analyze their resistance spectrum to Korean blast fungus population using 190 isolates collected from 1985 to 2002. Especially, the monogenic line containing Pi-9 gene was screened using 320 isolates. Based on the monogenic lines-blast isolate interactions, the 27 rice lines were classified into 9 groups. The chinese rice cultivar LTH showed susceptible to all the tested isolates. Those lines IRBLz-Fu, ERBL5-M and IRBL9-W harboring Pi-z, Pi-5, and Pi-9, respectively showed broader spectrum of resistance than those rice lines having Pi-19, Pi-7 etc. Interestingly, the Pi-9 gene(IRBL9-W) showed resistance to most isolates collected before 2000, but it showed susceptible reactions to 5％ and 20％ of blast fungus population in 2001 and 2002, respectively. Population of virulent isolates to Pi-ta, Pi-b, and Pi-7 also were increased in 2002 compared to those before 2000.

• Research in Plant Disease
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• v.21 no.1
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• pp.20-23
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• 2015

A sequential planting method was developed to screen rice plants with durable resistance against rice blast in a short time, and applied for several years in Korean rice breeding program. In this study, we showed the advantages of a sequential planting method compared to other pathogenicity tests. The correlation analysis among three pathogenicity tests and other factors demonstrated that durable resistance depended on the average of diseased leaf area and the number of compatible pathogens. Significant correlations were found in the nursery test but not in the field test result. In addition, we traced changes in the pathogen population during sequential planting stages through re-isolation of the pathogen. The portion of compatible pathogens was increased during sequential planting. Through this study, we provide an effective sequential planting method and direction of durable resistance in a breeding program.