• Title/Summary/Keyword: ribosomal RNA

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Phylogenetic Relationships of the Aphyllophorales Inferred from Sequence analysis of Nuclear Small Subunit Ribosomal DNA

  • Kim, Seon-Young;Jung, Hack-Sung
    • Journal of Microbiology
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    • v.38 no.3
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    • pp.122-131
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    • 2000
  • Phylogenetic classification of the Aphyllophorales was conducted based on the analysis of nuclear small subunit ribosomal RNA (nuc SSU rDNA) sequence. Based on phylogenetic groupings and taxonomic characters, 16 families were recognized and discussed. Although many of the characters had more or less homoplasies, miroscopic characters such ad the mitic system and clamp, spore amyloidity and rot type appeared to be important in the classification of the Aphyllophorales. Phylogenetically significant families were newly defined to improve the classification of the order Aphyllophorales.

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Inference of Genetic Regulatory Modules Using ChIP-on-chip and mRNA Expression Data

  • Cho, Hye-Young;Lee, Do-Heon
    • Bioinformatics and Biosystems
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    • v.2 no.2
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    • pp.62-65
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    • 2007
  • We present here the strategy of data integration for inference of genetic regulatory modules. First, we construct all possible combinations of regulators of genes using chromatin-immunoprecipitation(ChIP)-chip data. Second, hierarchical clustering method is employed to analyze mRNA expression profiles. Third, integration method is applied to both of the data. Finally, we construct a genetic regulatory module which is involved in the function of ribosomal protein synthesis.

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A Newly Recorded Sea Star of the Genus Marginaster (Asteroidea: Valvatida: Poraniidae) from the Korea Strait, Korea

  • Lee, Taekjun;Bae, Sungjun;Kim, Dae-Jin;Shin, Sook
    • Animal Systematics, Evolution and Diversity
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    • v.33 no.4
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    • pp.274-277
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    • 2017
  • A sea star was collected from the Korea Strait in the waters adjacent to eastern Jeju Island, Korea ($33^{\circ}39^{\prime}86^{{\prime}{\prime}}N$, $127^{\circ}33^{\prime}12^{{\prime}{\prime}}E$) at a depth of 92 m on November 5, 2016. This specimen was identified as Marginaster paucispinus Fisher, 1913, from the family Poraniidae of the order Valvatida, based on morphological characteristics. The genus Marginaster Perrier, 1881 and M. paucispinus, which were first reported in the South China Sea, are new to the Korean fauna. Partial sequences of mitochondrial COI and 16S ribosomal RNA of M. paucispinus were have been determined for the first time and were deposited in GenBank. They are the first molecular records for the genus Marginaster.

Taxonomic Studies of the Beta Hemolysis-causing Pathogen Bacillus cereus Isolated from Sea Water

  • Kim, Sam-Sun;Park, Yong-Ha;Lee, Jung-Sook;Yoon, Jung-Hoon;Shin, Yong-Kook;Rhee, In-Koo;Kim, Young-Jae
    • Journal of Microbiology and Biotechnology
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    • v.8 no.1
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    • pp.67-73
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    • 1998
  • A bacterial strain that excretes hemolysins and proteases into the growth medium was isolated from sea water and designated as KYJ 961. A nearly complete nucleotide sequence of a 16S ribosomal RNA gene from the isolate was determined following the isolation and cloning of amplified genes. On the basis of the 16S ribosomal DNA sequence data, and morphological, chemotaxonomic, and physiological characteristics, strain KYJ 961 was classified as a strain of Bacillus cereus.

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DNA Barcoding of Boccardiella hamata (Annelida: Polychaeta: Spionidae) in South Korea

  • Lee, Geon Hyeok;Yoon, Seong Myeong;Min, Gi-Sik
    • Animal Systematics, Evolution and Diversity
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    • v.36 no.3
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    • pp.268-273
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    • 2020
  • A spionid polychaete, Boccardiella hamata (Webster, 1879) has been found from mud in crevices between the shells of oysters and adherent substrates in South Korea. The sequences of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit 1 (CO1), 16S ribosomal DNA (16S), and the nuclear 18S ribosomal DNA (18S) from Korean individuals of Boccardiella hamata were determined in the present study. The molecular analysis based on the 18S rRNA gene sequences showed clear separation among the spionid polychaete species, and the sequences of Korean and Japanese individuals are completely identical. The morphological diagnosis and photographs of B. hamata are also provided.

Drug Resistance Effects of Ribosomal Protein L24 Overexpression in Hepatocellular Carcinoma HepG2 Cells

  • Guo, Yong-Li;Kong, Qing-Sheng;Liu, Hong-Sheng;Tan, Wen-Bin
    • Asian Pacific Journal of Cancer Prevention
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    • v.15 no.22
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    • pp.9853-9857
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    • 2014
  • Background: The morbidity and mortality rate of liver cancer continues to rise in China and advanced cases respond poorly to chemotherapy. Ribosomal protein L24 has been reported to be a potential therapeutic target whose depletion or acetylation inhibits polysome assembly and cell growth of cancer. Materials and Methods: Total RNA of cultured amycin-resistant and susceptible HepG2 cells was isolated, and real time quantitative RT-PCR were used to indicate differences between amycin-resistant and susceptible strains of HepG2 cells. Viability assays were used to determine amycin resistance in RPL24 transfected and control vector and null-transfected HepG2 cell lines. Results: The ribosomal protein L24 transcription level was 7.7 times higher in the drug-resistant HepG2 cells as compared to susceptible cells on quantitative RT-PCR analysis. This was associated with enhanced drug resistance as determined by methyl tritiated thymidine (3H-TdR) incorporation. Conclusions: The ribosomal protein L24 gene may have effects on drug resistance mechanisms in hepatocellular carcinoma HepG2 cells.