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http://dx.doi.org/10.5635/ASED.2020.36.3.037

DNA Barcoding of Boccardiella hamata (Annelida: Polychaeta: Spionidae) in South Korea  

Lee, Geon Hyeok (Department of Biological Sciences, Inha University)
Yoon, Seong Myeong (Department of Biology, College of Natural Sciences, Chosun University)
Min, Gi-Sik (Department of Biological Sciences, Inha University)
Abstract
A spionid polychaete, Boccardiella hamata (Webster, 1879) has been found from mud in crevices between the shells of oysters and adherent substrates in South Korea. The sequences of mitochondrial DNA (mtDNA) cytochrome c oxidase subunit 1 (CO1), 16S ribosomal DNA (16S), and the nuclear 18S ribosomal DNA (18S) from Korean individuals of Boccardiella hamata were determined in the present study. The molecular analysis based on the 18S rRNA gene sequences showed clear separation among the spionid polychaete species, and the sequences of Korean and Japanese individuals are completely identical. The morphological diagnosis and photographs of B. hamata are also provided.
Keywords
Spionidae; Boccardiella hamata; DNA barcode; CO1; 18S rRNA gene; Korea;
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1 Kessing B, Croom H, Martin A, McIntosh C, Owen McMillian W, Palumbi S, 1989. The simple fool's guide to PCR. Department of Zoology, University of Hawaii, Honolulu, HI, pp. 1-47.
2 Kim KS, Ji SJ, Min GS, 2020. COI DNA barcoding for Sterkiella multicirrata (Ciliophora: Oxytrichidae) from South Korea. Animal Systematics, Evolution and Diversity, 36:7-9. https://doi.org/10.5635/ASED.2020.36.1.025   DOI
3 Meisner K, Bick A, Bastrop R, 2011. On the identity of Spio filicornis (O.F. Muller, 1776)-with the designation of a neotype, and the description of two new species from the North East Atlantic Ocean based on morphological and genetic studies. Zootaxa, 2815:1-27. https://doi.org/10.11646/zootaxa.2815.1.1.1   DOI
4 Nishitani G, Nagai S, Hayakawa S, Kosaka Y, Sakurada K, Kamiyama T, Gojobori T, 2012. Multiple plastids collected by the dinoflagellate Dinophysis mitra through kleptoplastidy. Applied and Environmental Microbiology, 78:813-821. https://doi.org/10.1128/AEM.06544-11   DOI
5 Okuda S, 1937. Spioniform polychaetes from Japan. Journal of the Faculty of Science, Hokkaido Imperial University, Series VI, Zoology, 5:217-254.
6 Paik EI, 1989. Illustrated encyclopedia of fauna and flora of Korea. Vol. 31. Ministry of Education Press, Seoul, pp. 452-468.
7 Sato-Okoshi W, Okoshi K, 1997. Survey of the genera Polydora, Boccardiella and Boccardia (Polychaeta, Spionidae) in barkley sound (Vancouver Island, Canada), with special reference to boring activity. Bulletin of Marine Science, 60:482-493.
8 Radashevsky VI, 1993. Revision of the genus Polydora and related genera from the north west Pacific (Polychaeta: Spionidae). Publications of the Seto Marine Biological Laboratory, 36:1-60.   DOI
9 Sato-Okoshi W, 2000. Polydorid species (Polychaeta: Spionidae) in Japan, with descriptions of morphology, ecology and burrow structure. 2. Non-boring species. Journal of the Marine Biological Association of the United Kingdom, 80:443-456. https://doi.org/10.1017/S0025315499002143   DOI
10 Sato-Okoshi W, Abe H, 2012a. Morphological and molecular sequence analysis of the harmful shell boring species of Polydora (Polychaeta: Spionidae) from Japan and Australia. Aquaculture, 368-369:40-47. https://doi.org/10.1016/j.aquaculture.2012.08.046   DOI
11 Simon CA, Sato-Okoshi W, Abe H. 2019. Hidden diversity within the cosmopolitan species Pseudopolydora antennata (Claparede, 1869) (Spionidae: Annelida). Marin Biodiversity, 49:25-42. https://doi.org/10.1007/s12526-017-0751-y   DOI
12 da Silva JM, Willows-Munro S, 2016. A review of over a decade of DNA barcoding in South Africa: a faunal perspective. African Zoology, 51:1-12. https://doi.org/10.1080/15627020.2016.1151377   DOI
13 Teramoto W, Sato-Okoshi W, Abe H, Nishitani G, Endo Y, 2013. Morphology, 18S rRNA gene sequence and life history of a new Polydora species (Polychaeta: Spionidae) from northeastern Japan. Aquatic Biology, 18:31-45. https://doi.org/10.3354/ab00485   DOI
14 Walker LM, 2011. A review of the current status of the Polydora complex (Polychaeta: Spionidae) in Australia and a checklist of recorded species. Zootaxa, 2751:40-62. https://doi.org/10.11646/zootaxa.2751.1.3   DOI
15 Webster HE, 1879. The Annelida Chaetopoda of the Virginian coast. Transactions of the Albany Institute, 9:202-269.
16 Abe H, Kondoh T, Sato-Okoshi W, 2016. First report of the morphology and rDNA sequences of two Pseudopolydora species (Annelida: Spionidae) from Japan. Zoological Science, 33:650-658.   DOI
17 Clare EL, Lim BK, Engstrom MD, Eger JL, Hebert PDN, 2007. DNA barcoding of Neotropical bats: species identification and discovery in Guyana. Molecular Ecology Notes, 7:184-190. https://doi.org/10.1111/j.1471-8286.2006.01657.x   DOI
18 Kerckhof F, Faasse MA, 2014. Boccardia proboscidea and Boccardiella hamata (Polychaeta: Spionidae: Polydorinae), introduced mud worms new for the North Sea and Europe, respectively. Marine Biodiversity Records, 7:e76. https://doi.org/10.1017/S1755267214000803   DOI
19 Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35:1547-1549. https://doi.org/10.1093/molbev/msy096   DOI
20 Kimura M, 1980. A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences. Journal of Molecular Evolution, 16:111-120. https://doi.org/10.1007/BF01731581   DOI
21 Paik EI, 1982. Taxonomic studies on polychaetous annelids in Korea. Research Bulletin of the Hyosung Women's University, 24:745-913.
22 Radashevsky VI, Pankova VV, 2013. Shell-boring versus tube-dwelling: is the mode of life fixed or flexible? Two cases in spionid polychaetes (Annelida, Spionidae). Marine Biology, 160:1619-1624. https://doi.org/10.1007/s00227-013-2214-8   DOI
23 Blake JA, 1983. Polychaetes of the family Spionidae from South America, Antarctica and adjacent seas and islands. In: Biology of the Antarctic Seas XIV. Antarctic Research Series, 39:205-288.   DOI
24 Sato-Okoshi W, Abe H, 2013. Morphology and molecular analysis of the 18S rRNA gene of oyster shell borers, Polydora species (Polychaeta: Spionidae), from Japan and Australia. Journal of the Marine Biological Association of the United Kingdom, 93:1279-1286. https://doi.org/10.1017/S002531541200152X   DOI
25 Sato-Okoshi W, Okoshi K, Koh BS, Kim YH, Hong JS, 2012b. Polydorid species (Polychaeta, Spionidae) associated with commercially important mollusk shells in Korean waters. Aquaculture, 350-353:82-90. https://doi.org/10.1016/j.aquaculture.2012.04.013   DOI
26 Tamura K, Nei M, 1993. Estimation of the number of nucleotide substitution in the control regions of mitochondrial DNA in humans and chimpanzees. Molecular Biology and Evolution, 10:512-526. https://doi.org/10.1093/oxfordjournals.molbev.a040023
27 Zhou J, Ji W, Li X, 2010. Records of Polydora complex spionids (Polychaeta: Spionidae) from China's coastal waters, with emphasis on parasitic species and the description of a new species. Marine Fisheries, 32:1-15.   DOI
28 Williams LG, Karl SA, Rice S, Simon C, 2017. Molecular identification of polydorid polychaetes (Annelida: Spionidae): is there a quick way to identify pest and alien species? African Zoology, 52:105-117. https://doi.org/10.1080/15627020.2017.1313131   DOI
29 Berkeley E, 1927. Polychaetous annelids from the Nanaimo district: Part 3. Leodicidae to Spionidae. Contributions to Canadian Biology and Fisheries, 3:407-422. https://doi.org/10.1139/f26-017
30 Blake JA, 1966. On Boccardia hamata (Webster), new combination (Polychaeta: Spionidae). Bulletin of the Southern California Academy of Sciences, 65:176-184.
31 Blake JA, Kudenov JD, 1978. The Spionidae (Polychaeta) from southeastern Australia and adjacent areas with a revision of the genera. Memoirs of the Natural Museum of Victoria, 39:171-280.   DOI
32 Hartman O, 1969. Atlas of the sedentariate polychaetous annelids from California. Allan Hancock Foundation, University of Southern California, Los Angeles, CA, pp. 1-812.
33 Hebert PDN, Cywinska A, Ball SL, deWaard JR, 2003. Biological identifications through DNA barcodes. Proceedings of the Royal Society B, Biological Sciences, 270:313-321. https://doi.org/10.1098/rspb.2002.2218
34 Imajima M, Hartman O, 1964. The polychaetous annelids of Japan. Part II. Allan Hancock Foundation Occasional Papers, 26:239-452.