• The Korean Journal of Malacology
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• v.12 no.2
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• pp.85-90
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• 1996

3종의 복족류(Rapana venosa, Reishia bronni, Anthosiphonaria sirius)와 1종의 다판류, Lepidosona(Lepidosona) coreanica에 대한 18S ribosomal DNA의 염기서열을 밝히고 이들을 이미 보고된 18종의 이매패류, 2종의 복족류 그리고 1종의 다판류의 염기서열과 비교분석하였다. 그 셜과 복족류는 V4 region에서 다른 연체동물과 구별되는 독특한 inseerted sequinces를 가지고 있었으며, V2 region에서 복족류(Prosobranchia와 Pulmonata)와 이매패류(Pteriomorphia 와Heteerodonta) 각각의 두 아강들이 서로 다른 특징적인 insertions 또는 deletions으로 구분되었다.

• Mycobiology
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• v.29 no.2
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• pp.73-79
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• 2001

The Polyporaceae is a chaotic mass of genera having poroid hymenophores in the Aphyllophorales. To classify the Polyporaceae into more natural groups, phylogenetic analyses were performed using nuclear small subunit ribosomal DNA sequences. Thirty-six species from the families of the Polyporaceae, the Hymenochaetaceae, the Ganodermataceae, the Corticiaceae, the Bondarzewiaceae, the Meruliaceae, the Steccherinaceae and the Lentinaceae were phylogenetically compared. By performing maximum parsimony analysis, seven phylogenetically meaningful groups were identified and discussed. The hyphal system, presence or absence of clamps, and the type of rot were found as important characters in defining the groups. Each group was phylogenetically significant enough to be a core member of each family when the Polyporaceae was split into smaller and more natural families.

• Journal of Sericultural and Entomological Science
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• v.43 no.1
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• pp.1-8
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• 2001

Nucleotide sequence in internal transcribed spacer (ITS) regions of ribosomal DNA among mulberry varieties (Morus species) were analyzed in order to identify the possibility of classification for the species. The variations in the ITS regions were compared among 9 mulberry varieties and one variety of Cudrania species as an outgroup. ITS 1 region of the varieties ranging from 219 to 220 bp in length was 49-50 bp shorter than ITS 2 region. Of 510 sites in the ITS 1 and 2 regions, 148 sites were potentially variable, of which 52% and 48% sites were distributed in ITS 1 and ITS 2 regions, respectively. By pairwise comparisons on the nucleotide sequences in the ITS 1 and 2 regions among 9 mulberry varieties, they were classified into 5 groups. Divergence values of the sequences, however, were considerably low ranging from 0 to 1.3%. Especially, there was no divergence among Backasipmunja, Chungilppong and Milsungpong and Jungyasang, Ssarigol II and Yulbon, respectively.

• YAKHAK HOEJI
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• v.24 no.1
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• pp.1-10
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• 1980

Viomycin inhibited polypeptide biosynthesis, initiation complex formation and translocation of peptidyl-tRNA on ribosomes derived from a sensitive strain of Mycobacterium smegmatis (R-15), but not significantly on ribosomes from viomycin-resistant mutants(R-31 and R-43). The inhibition of translocation was stronger than that of initiation complex formation in the sensitive strain. The binding of [$^{14}C$] tuberactinomycin O, a viomycin analog, to ribosomal particles was studied by Millipore filter method. The sensitive ribosome exhibited higher affinity for the antibiotic than the resistant ribosomes. The resistance was localized on the large ribosomal subunit in a mutant(R-31), and on the small subunit in another mutant(R-43). The binding of the drug to the sensitive ribosomal subunit was markedly reduced by combination with the resistant pair subunit, and the entire ribosome became resistant to the antibiotic.

• Parasites, Hosts and Diseases
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• v.41 no.1
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• pp.47-55
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• 2003

We compared patterns of intraspecific polymorphism of two markers with contrasting modes of evolution, nuclear ribosomal DNA (rDNA) and mitochondrial DNA (mtDNA), in the lung fluke, diploid and triploid Paragonimus westermani from three geographical regions of Korea. The genetic distances between three populations of Korean diploid and triploid P. westermani showed no significant difference in the nucleotide sequences of the mitochondrial cytochrome c oxidase subunit 1 (mtCOI) and ribosomaal second internal transcribed spacer (ITS2) genes. A highly resolved strict-consensus tree was obtained that illustrated phylogenetically useful information of the ITS2 and mtCOI sequences from diploid and triploid P. westermani.

• Korean Journal of Environmental Biology
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• v.27 no.1
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• pp.95-99
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• 2009

Transcriptomic changes in the brain of Limanda yokohamae were investigated to understand the environmental condition of Masan Bay, Korea. Differentially expressed genes (DEGs) in the brain of the flat fish from Masan Bay were identified by comparing those from the reference site Gangneung using annealing control primers-based polymerase chain reaction. The results demonstrated that two different kinds of the cytoplasmic ribosomal proteins, 40 s ribosomal protein S27a and ribosomal protein L6, were identified by the BLAST searching followed by sequence analysis. These findings suggest that environmental status of Masan Bay could hinder protein synthesis that is required for maintaining brain functions and thus cause the dysfunction of fish physiology.

• Journal of Sericultural and Entomological Science
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• v.41 no.3
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• pp.174-179
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• 1999

The genetic relationships among six Cordyceps spp. were investigated based on internal transcribed spacer sequences of ribosomal DNA .A portion of the these genes was amplified by PCR. Approximately 590 base pairs were successfully amplified, cloned, sequenced, compared. The nucleotide sequence of the six amplified fragments were aligned by the clustal W program. As a result, Cordyceps militaris shared 87, 96, 98, 90 and 97% sequences homology with Paecilomyces japonica, Paecilomyces sp. J300, Paeciomyces farinosa. Paecilomyces sp. J500 and Cordyceps sinensis, respectively. Paecilomyces japonica also shared 87, 88, 92 and 87% sequence similarity with Paecilomyces sp. J300, Paecilomyces farinosa, Paecilomyces sp. J500 and Cordyceps sinensis, repectively.

• Journal of Life Science
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• v.25 no.9
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• pp.1007-1013
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• 2015

A COG (Cluster of Orthologous Groups of proteins) algorithm was applied to detect conserved genes in 711 prokaryotes. Only COG0080 (ribosomal protein L11) was common among all the 711 prokaryotes analyzed and 58 COGs were common in more than 700 prokaryotes. Nine COGs among 58, including COG0197 (endonuclease III) and COG0088 (ribosomal protein L4), were conserved in a form of one gene per one organism. COG0008 represented 1356 genes in 709 of the prokaryotes and this was the highest number of genes among 58 COGs. Twenty-two COGs were conserved in more than 708 prokaryotes. Of these, two were transcription related, four were tRNA synthetases, eight were large ribosomal subunits, seven were small ribosomal subunits, and one was translation elongation factor. Among 58 conserved COGs in more than 700 prokaryotes, 50 (86.2%) were translation related, and four (6.9%) were transcription related, pointing to the importance of protein-synthesis in prokaryotes. Among these 58 COGs, the most conserved COG was COG0060 (isoleucyl tRNA synthetase), and the least conserved was COG0143 (methionyl tRNA synthetase). Archaea and eubacteria were discriminated in the genomic analysis by the average distance and variation in distance of common COGs. The identification of these conserved genes could be useful in basic and applied research, such as antibiotic development and cancer therapeutics.

• KSBB Journal
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• v.22 no.5
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• pp.362-365
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• 2007

Cyanobacteria have been identified as one of the most promising group producing novel biochemically active natural products. Cyanobacteria are a very old group of prokaryotic organisms that produce very diverse secondary metabolites, especially non-ribosomal peptide and polyketide structures. Large multienzyme complexes which are responsible for the non-ribosomal biosynthesis of peptides are modular for the addition of a single amino acid. An activation of amino acid substrates results in an amino adenylate occuring via an adenylation domain (A-domain). A-domains are responsible for the recognition of amino acids as substrates within NP synthesis. The A-domain contains ten conserved motifs, A1 to A10. In this study, ten conserved motifs from A1 to A10 were checked regarding their amino acid sequence of the NRPS-module of Microcystis aeruginosa PCC 7806. The part of the amino acid sequence chosen was that which contained as many conserved motives as possible, and then these amino sequence were compared between other cyanobacteria to design a new degenerate primer. A new degenerate primer (A3/A7 primer) was designed to detect any putative NP synthetase region in unkwon cyanobacteria by a reverse translation of the conserved amino acid sequence and a search for cyanobacterial DNA bank.

• Journal of Microbiology
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• v.38 no.3
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• pp.122-131
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• 2000

Phylogenetic classification of the Aphyllophorales was conducted based on the analysis of nuclear small subunit ribosomal RNA (nuc SSU rDNA) sequence. Based on phylogenetic groupings and taxonomic characters, 16 families were recognized and discussed. Although many of the characters had more or less homoplasies, miroscopic characters such ad the mitic system and clamp, spore amyloidity and rot type appeared to be important in the classification of the Aphyllophorales. Phylogenetically significant families were newly defined to improve the classification of the order Aphyllophorales.