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Identification of Differentially Regulated Genes in the Brain of Limanda yokohamae from Masan Bay, Korea  

Oh, Jeong-Hwan (Department of Biological Sciences, Pusan National University)
Moon, Hyo-Bang (Marine Environment Research Team, National Fisheries Research and Development Institute)
Choe, Eun-Sang (Department of Biological Sciences, Pusan National University)
Publication Information
Korean Journal of Environmental Biology / v.27, no.1, 2009 , pp. 95-99 More about this Journal
Abstract
Transcriptomic changes in the brain of Limanda yokohamae were investigated to understand the environmental condition of Masan Bay, Korea. Differentially expressed genes (DEGs) in the brain of the flat fish from Masan Bay were identified by comparing those from the reference site Gangneung using annealing control primers-based polymerase chain reaction. The results demonstrated that two different kinds of the cytoplasmic ribosomal proteins, 40 s ribosomal protein S27a and ribosomal protein L6, were identified by the BLAST searching followed by sequence analysis. These findings suggest that environmental status of Masan Bay could hinder protein synthesis that is required for maintaining brain functions and thus cause the dysfunction of fish physiology.
Keywords
ACP-based PCR; marine environmental contamination; Masan Bay; Limanda yokohamae;
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