• 한국미생물·생명공학회지
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• 제15권2호
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• pp.112-115
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• 1987

B. megaterium ATCC 13639와 E. aerogenges의 비타민 B$_{12}$ 생산을 위한 최적 pH는 각각 6.0과 5.0으로 pH의 영향을 크게 받았으나 두 세균에 의한 riboflavin 생산은 pH에 따른 변화가 거의 없었다. Sucrose를 첨가하면 두 비타민 생산량이 크게 증가되었고 최적pH도 변함을 알 순 있었다. 두 세균에 의한 두 비타민 생산에 미치는 온도의 영향은 pH에 비하여 아주 적었다. 이상의 결론은 장차 여러 가지 식품 폐기물을 이용한 비타민 B$_{12}$와 riboflavin의 생산이 가능함을 보여주었다.

• Applied Biological Chemistry
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• 제36권5호
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• pp.332-338
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• 1993

리보플라빈을 생산하기 위하여 Ashbya gosspii NRRL Y-1056의 균주개량을 시도하였으며 선발한 변이주 Ashbua gossypii JAG-13에 의한 리보플라빈의 생산시의 최적 배지조성 및 배양조건을 결정하였다. 최적 배지조성은 9%의 옥수수 기름, 3%의 젤라톤, 4%의 CSL, 0.3%의 글리신, 0.2%의 자당 지방산 에스테르 S770 였다. 배양배지의 초기 pH와 최적 배양온도는 각각 pH 6.5 와 $28^{\circ}C$였다. 산소의 공급은 리보플라빈의 생산에 필수적이었으나 과도한 산소의 공급은 오히려 리보플라빈의 생산을 저해하였다. Ashbya gosspii JAG-13을 위와같은 조건에서 생물배양기를 이용하여 12일간 배양하여 6.9 mg/ml의 리보플라빈을 생산하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제6권2호
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• pp.75-88
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• 2001

In this paper, the microbial production of riboflavin is reviewed and includes descriptions of riboflavin overproducers, and the biosynthesis and details of the key-enzyme genes related to riboflavin. There kinds of riboflavin overproducers are known; Bacillus subtilis and Candida famate utilize glucose as a carbon source, but the fungus Ashbya gossypii requires plant oil as its sole carbon source. The starting material in ribofalvin biosynthesis is guanosine triphospate (GTP), which is converted to riboflavin through six enzymatic reactions. Though Bacillus subtilis, Candida famate, and Ashbya gossypii operate via different pathways until GTP, they follow the same pathway from GTP to riboflavin. From the metabolic viewpoint, with respect to improved riboflavin production, the supplementation of GTP, aprocess-limiting precursor must be considered. The GTP fluxes originate from three sources, serine, threonine and glyoxylate cycles. The development of pathways to strengthen GTP supplementation using biotechnological techniques remains an issue fro future research.

• 한국미생물·생명공학회지
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• 제21권6호
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• pp.615-621
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• 1993

In order to maximize the riboflavin production by a mutant strain Ashbya gosspyii, the optimization of medium and fed-batch fermentation were performed. As carbon sources, glucose and soybean oil were necessary for the riboflavin overproduction. Optimal concentrations of glucose and soybean oil in the flask cultures were found to be 3.0% and 0.5%, respectively, in a complex medium containing corn steep liquor(CLS) 1%. Among the various organic nitrogen sources tested, CSL was the most effective one both for the cell growth and riboflavin overproduction.

• Journal of Microbiology and Biotechnology
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• 제11권4호
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• pp.552-557
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• 2001

By UV-irradiation of Eremothecium ashbyii DTl, a higWy flavinogenic mutant (UV-18-57) and a nonflavinogenic mutant (UV -85) were obtained. The physio-morphological characteristics of these three strains were studied on glucose medium in submerged fermentation. Glucose utilization and mycelial growth occurred in 0 - 2 days of fermentation. By the third day, the biomass had declined. Extracellular riboflavin excretion was distinct from the second day, reaching a maximum rate by the fourth day. The hyphae of the highly flavinogenic mutant UV-18-57 were broader than DTl, while the nonflavinogenic UV-85 hyphae were very thin. Riboflavin accumulation was high in UV-18-57 (extracellular riboflavin,$825\mu\textrm{g}/ml$ , and intracellular, $490\mu\textrm{g}/ml$) and caused the mycelia to swell into bulbous forms. Riboflavin accumulation was less in DTl ($108\mu\textrm{g}/ml$ extracellular and $24\mu\textrm{g}/ml$ intracellular) and correspondingly its hyphae were thinner than those of UV-18-57 and swollen bulbous mycelia were not prominent. UV-85 was nonflavinogenic and, accordingly, its mOlphological characteristics included long thin filaments with no intracellular riboflavin accumulation. A large number of greenish fluorescence spores were seen in UV-18-57, whereas DTI had less spores and UV-85 was nonsporulating. Sporulation is correlated with riboflavin production. UV-18-57 had better mycelial integrity and lysis started only by the seventh day, whereas DTI and UV -85 started to lyze earlier by 4 -5 days. By the late stage of fermentation (eighth day), DTl had a few long, thin filaments indicating some secondary growth, whereas UV -85 showed a compact pellet form of mycelia. Most mycelia of UV-18-57 still appeared intact.

• 한국미생물·생명공학회지
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• 제17권3호
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• pp.207-212
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• 1989

토양으로부터 분리 한 균주 YS-221-B로부터 $\alpha$-D-Glucosidase Inhibitor 생산조건을 검토한 결과는 다음과 같다. 최적 pH와 온도는 8.0, 3$0^{\circ}C$였으며, 탄소원으로는 mannitol, 1-Inositol, erythritol과 같은 당알코올이 양호하였으며, 질소원으로는 asparagine, beef extract가 양호하였다. Riboflavin, folic acid, thiamine과 같은 vitamin의 첨가에 의해서 저해물질 생산이 10~20% 증가되었으며, 금속이온 중에 $Zn^{++}$, $Mg^{++}$은 약 20% 정도 물질생산을 촉진시켰으나 Li$^+$, Co$^{++}$ $Ca^{++}$, Fe$^{++}$ 등은 감소시켰다. 그리고 pH8.0, 3$0^{\circ}C$에서 8~9일간 배양함으로써 저해물질 생산이 최고에 달하였다.

• 한국식생활문화학회지
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• 제39권1호
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• pp.74-81
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• 2024

Ulcerative colitis (UC) is a chronic inflammatory intestinal disease characterized by an imbalance in immune function and the overexpression of inflammatory cytokines and mediators. Vitamin B2, also known as riboflavin (Libof), is an essential water-soluble vitamin with numerous beneficial properties, including antioxidant, anti-aging, anti-inflammatory, anti-nociceptive, and anti-cancer effects. In this study, we aimed to investigate the protective effects of Libof on dextran sulfate sodium (DSS)-induced experimental colitis. The C57BL/6 mice were used as the in vivo model of chronic colitis to investigate the anti-inflammatory effects of Libof. RAW 264.7 cells were used for the in vitro investigation of the molecular mechanisms underlying these effects. In vivo, Libof alleviated the DSS-induced disease activity index (DAI), colon length shortening, and colonic pathological damage. In vitro, Libof inhibited lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 production in RAW 264.7 cells. Moreover, Libof inhibited LPS-induced nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) expression in RAW 264.7 cells. In conclusion, these findings indicate that Libof shows potential as an agent for the treatment of UC.

• Asian-Australasian Journal of Animal Sciences
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• 제32권12호
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• pp.1873-1881
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• 2019

Objective: Information regarding the vitamin content of silage is limited. This study investigated the changes in the vitamin content of alfalfa and Chinese leymus silages with or without a lactic acid bacterial inoculant. Methods: Alfalfa at the early flowering stage and Chinese leymus at the full-bloom stage were harvested. The treatments for each forage type were control (deionized water only) and $1{\times}10^6$ colony-forming units Lactobacillus plantarum (LP)/g fresh matter. After 45 days of ensiling, all silages were sampled for evaluating the vitamin content, fermentation quality and chemical composition. Results: The LP inoculant decreased the pH value and ammonia nitrogen content of the alfalfa and Chinese leymus silages and significantly (p<0.05) increased the lactic acid, acetic acid concentrations and Flieg's points. Prior to ensiling, the levels of five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) and ${\alpha}$-tocopherol in alfalfa were significantly (p<0.01) higher than those in Chinese leymus. Ensiling decreased the levels of the five B-group vitamins in both alfalfa and Chinese leymus while increasing the ${\alpha}$-tocopherol content of Chinese leymus. The thiamin, riboflavin, niacin and pantothenic acid levels in the LP-treated silage were significantly (p<0.05) lower than those in the untreated silage for the alfalfa and Chinese leymus. The ${\alpha}$-tocopherol content in the LP-treated alfalfa silage was significantly (p<0.05) higher than that in the untreated alfalfa silage. There was no significant (p>0.05) difference in pyridoxine content between the untreated and LP-treated silages for both forages. Conclusion: With or without LP inoculation, the levels of the five B-group vitamins (thiamin, riboflavin, niacin, pantothenic acid, and pyridoxine) in alfalfa and Chinese leymus decreased after 45 days of ensiling, while the ${\alpha}$-tocopherol content of Chinese leymus increased. The LP inoculant improved the fermentation quality of both the alfalfa and Chinese leymus silages but increased the thiamin, riboflavin, niacin, and pantothenic acid loss in the two forages after fermentation.

• 한국미생물·생명공학회지
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• 제20권2호
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• pp.183-189
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• 1992

Bacteriocin 생산균주인 Lactococcus sp. 111-2의 최적 배양조건과 배양 특성을 검토하였다. 탄소원은 glucose 20g/l, 질소원은 casein acid hydrolyzate 15g/l, 무기염류는 sodium acetate 3g/l와 ammonium citrate 2g/l가 bacteriocin 생성에 많은 영향을 주는 것으로 타났으며, 배지 pH는 6.2, 발효온도는 $35^{\circ}C$일 때 높은 생성수율을 보였다. 선발균주는 증식 및 항균물질의 생성에 riboflavin과 pantotheinc acid의 비타민을 요구하였다. 회분식 발효시 항균성물질의 생산패턴은 배양 12시간에 3,894IU/ml로 최대를 나타냈으며 균체생성은 배양 8.5시간에 정상기에 도달하였다.

• 식물조직배양학회지
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• 제24권3호
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• pp.143-148
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• 1997

모상근의 성장 및 tropane alkaloids의 생성에 미치는 pH, 서당, 비타민의 영향을 구명하기 위하여, 독말풀(Datura stramonium var. tatula Torr.)의 잎에 Agrobacterium tumefaciens $A_4$T를 접종하여 모상근을 유도하였다. 유도된 모상근 중 성장률이 양호한 clone (DTLA9)을 선발하고, 이를 pH, 서당, 비타민을 각각 여러 농도로 처리한 SH(Schenk and Hildebrandt, 1972) 기본배지에 배양하였다. 모상근의 성장에 있어 최적 pH는 6.3이었으며, 최적 서당농도는 3.0%이었다. Tropane alkaloids의 함량에 있어 최적 PH는 6.5이었으며, 최적 서당농도는 2.8%이었다. 한편, 비타민이 제거된 SH기본배지에 ascorbic acid, D-pantothenate, nicotinic acid, pyridoxine, riboflavin. 그리고 thiamine을 각각 농도별로 첨가하여 배양한 경우에, 모상근의 성장에 있어 비타민의 최적 농도는 각각 0.1, 0.003, 0.07, 0.002, 0.025, 0.01 mM이었다. Tropane alkaloids의 함량은 0.1 mM의 ascorbic acid 단독처리구에서 대조구(vitamin-free구) 및 SH기본배지에서의 경우에 비하여 가장 높게 나타났다.