• 대한수의학회지
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• 제53권2호
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• pp.137-141
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• 2013

To report retinal image of in a 6-year-old male castrated poodle dog with a 2-month history of nyctalopia using optical coherence tomography (OCT). Ocular reflexes were present in both eyes and slit lamp examination showed anterior subscapular cataract in the right eye. There were fundus abnormalities in both eyes similar to retinal degeneration. Scotopic electroretinograms (ERGs) revealed significantly subabnormal amplitudes and prolonged implicit time, whereas photopic ERGs were better maintained, although far from normal. OCT of affected dogs revealed generalized retinal thinning much more than a normal age-matched dog. Therefore, OCT scanning is considered to be a useful method for retinal evaluation in dogs with retinal degeneration.

• 한국안광학회지
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• 제21권2호
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• pp.137-146
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• 2016

목적: 중심장액성맥락망막병증을 가진 눈의 망막 두께 및 망막 부피와 같은 망막 상태와 시력과의 상관관계를 알아보았다. 방법: 총 78명 136안을 대상으로 중심장액성맥락망막병증을 치료 중인 눈 및 무증상 반대편 눈, 중심장액성맥락망막병증을 진단받은 후 완치된 눈 및 반대편 눈과 정상인 눈 각각의 나안시력, 최대교정시력, 망막 두께, 망막 부피를 측정하였다. 결과: 중심장액성맥락망막병증을 치료 중인 눈은 나안시력, 최대교정시력, 망막 두께, 망막 부피 모두에서 반대편 무증상 눈, 질환이 완치된 눈 및 정상 눈과 비교하여 통계적으로 유의하게 차이가 있었다. 중심장액성맥락망막병증의 반대편 무증상 눈의 최대교정시력은 정상 눈과 차이가 없었으나 망막 두께 및 망막 부피는 정상 눈과 통계적으로 유의한 차이가 있었다. 망막 두께가 증가함에 따라 망막 부피가 증가하는 정도가 정상안의 경우보다 중심장액성맥락망막병증 소견을 보이는 눈이나 반대편 무증상 눈, 중심장액성맥락망막병증이 완치된 눈 및 그 반대편 눈에서 더 컸다. 결론: 본 연구를 통하여 중심장액성맥락망막병증을 가지고 있는 눈 뿐만 아니라 반대편 무증상 눈에서도 망막 두께 및 망막 부피가 증가하며, 중심장액성맥락망막병증에 의한 최대교정시력의 변화는 일정 정도 이상의 망막 두께 및 망막 부피가 증가되었을 때 나타남을 밝혔다.

• 생명과학회지
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• 제22권7호
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• pp.863-870
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• 2012

세포막 단백질 중 가장 큰 family를 형성하는 G protein-coupled receptor (GPCR)는 세포 외부의 다양한 신호를 세포 내 G 단백질의 활성화를 통하여 전달한다. 외부 신호자극이 없는 조건에서도 활성을 나타내는 항활성 돌연변이(constitutively active mutants, CAM)는 GPCR 신호전달 이상으로 인한 질병 치료나 GPCR의 활성화 구조연구에 좋은 대상이다. 본 연구는 시각수용체 로돕신에서 약한 항활성을 보이는 CAM의 하나인 E134Q/M257Y를 대상으로, inverse agonist와 agonist 존재 하에서 형성하는 두 가지 chromophore의 특성을 연구하였다. 이 CAM은 11-cis-retinal과 all-trans-retinal 존재 하에서 각기 최대흡광도가 500 nm와 380 nm인 로돕신을 형성한다. 두 가지 retinal을 다양한 비로 혼합한 조건과 연속적으로 결합하는 조건 하에서 각 형태의 로돕신 형성을 조사한 결과 E134Q/M257Y mutant는 11-cis-retinal과 우선적으로 결합함을 보여준다. E134Q/M257Y mutant는 wild type 옵신에 비해 11-cis-retinal에 대한 친화도는 별다른 차이가 없으나 옵신과 로돕신 상태의 안정성이 낮음이 확인되었다. 본 연구 결과는 GPCR의 활성화 시 일어나는 부분적 구조변화에 대한 정보를 제공하고, 구조정보에 기반한 GPCR신호를 미세하게 조절하는 물질의 발굴이나 개발에 유용하게 이용될 것이다.

• International Journal of Contents
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• 제2권3호
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• pp.27-32
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• 2006

We present an effective algorithm for automatic tracing of retinal vessel structure and vascular landmark extraction of bifurcations and ending points. In this paper we deal with vascular patterns from RGB images for personal identification. Vessel tracing algorithms are of interest in a variety of biometric and medical application such as personal identification, biometrics, and ophthalmic disorders like vessel change detection. However eye surface vasculature tracing in RGB images has many problems which are subject to improper illumination, glare, fade-out, shadow and artifacts arising from reflection, refraction, and dispersion. The proposed algorithm on vascular tracing employs multi-stage processing of ten-layers as followings: Image Acquisition, Image Enhancement by gray scale retinal image enhancement, reducing background artifact and illuminations and removing interlacing minute characteristics of vessels, Vascular Structure Extraction by connecting broken vessels, extracting vascular structure using eight directional information, and extracting retinal vascular structure, and Vascular Landmark Extraction by extracting bifurcations and ending points. The results of automatic retinal vessel extraction using jive different thresholds applied 34 eye images are presented. The results of vasculature tracing algorithm shows that the suggested algorithm can obtain not only robust and accurate vessel tracing but also vascular landmarks according to thresholds.

• 대한수의학회지
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• 제58권4호
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• pp.219-221
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• 2018

A 2-year-old intact female Bengal cat was presented with a 6-month history of visual impairment. The cat manifested bilateral negative menace responses and dazzle reflexes and sluggish pupillary light reflexes. Bilateral fundus changes included generalized tapetal hyperreflectivity, advanced retinal vascular attenuation, and increased pallor of the optic disc. A diagnosis of bilateral retinal degeneration was made. The clinical findings suggest that the investigated Bengal cat was most likely to have an inherited retinal degeneration. Further studies of the Bengal cat breed are needed to determine the prevalence of inherited retinal degeneration in this breed in Korea.

• International Journal of Computer Science & Network Security
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• 제24권8호
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• pp.169-173
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• 2024

Nowadays high security is an important issue for most of the secure places and recent advances increase the needs of high-security systems. Therefore, needs to high security for controlling and permitting the allowable people to enter the high secure places, increases and extends the use of conventional recognition methods. Therefore, a novel identification method using retinal images is proposed in this paper. For this purpose, new mathematical functions are applied on corners and bifurcations. To evaluate the proposed method we use 40 retinal images from the DRIVE database, 20 normal retinal image from STARE database and 140 normal retinal images from local collected database and the accuracy rate is 99.34 percent.

• 인지과학
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• 제14권3호
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• pp.1-11
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• 2003

정지/운동하는 배경자극들 속에서 정지/운동하는 표적을 탐지하는 데 있어서 표적 유형과 망막 이심율의 효과를 알아보기 위해서 두 가지 실험을 수행하였다. 두 실험 모두 시각탐색과제를 사용하였다. 망막 이심율은 1.6$^{\circ}$ 단위로 커지는 5개의 동심원으로 구분하였으며, 표적은 배경자극과 방위차원에서 차이나는 방위 표적과 세부특정에서 차이나는 세부특정 표적이었다. 실험 l에서는 표적과 배경자극이 모두 정지되어있는 상황에서의 탐색을 다루었다. 그 결과 표적 유형과 망막 이심율 사이에 상호작용이 있었다. 정지상황에서 방위 표적은 망막 이심율의 영향을 별로 받지 않는 반면에, 세부특정 표적은 망막 이율이 증가함에 따라서 탐지시간이 일관성 있게 증가하였다. 표적과 배경자극이 모두 운동하는 상황인 실험 2 에서도 둘 사이의 상호작용이 나타냈으나, 그 이유는 실험 1과 극적인 대조를 이루었다. 즉, 운 동 상황에서 방위 표적은 망막 이심율이 증가함에 따라서 탐지시간이 일관성 있게 감소하는 반면, 세부 특징 표적은 망막 이심율의 영향을 거의 받지 않았다. 두 실험의 결과를 항공기나 자동차의 운동과 같은 현실상황과 관련된 합의와 문제점 그리고 향후 연구방향을 논의하였다.

• Clinical and Experimental Reproductive Medicine
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• 제38권4호
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• pp.216-221
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• 2011

Objective: To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases. Methods: The embryoid bodies were formed from hESCs and attached on the matrigel coated culture dishes. The neural structures consisting neural precursors were selected and expanded to form rosette structures. The mechanically isolated neural rosettes were differentiated into pigmented cells in the media comprised of N2 and B27. Expression profiles of markers related to RPE development were analyzed by reverse transcription-polymerase chain reaction and immunostaining. Dissociated putative RPE cells ($10^5$ cells/5 ${\mu}L$) were transplanted into the subretinal space of rat retinal degeneration model induced by intravenous sodium iodate injection. Animals were sacrificed at 1, 2, and 4 weeks after transplantation, and immnohistochemistry study was performed to verify the survival of the transplanted cells. Results: The putative RPE cells derived from hESC showed characteristics of the human RPE cells morphologically and expressed molecular markers and associated with RPE fate. Grafted RPE cells were found to survive in the subretinal space up to 4 weeks after transplantation, and the expression of RPE markers was confirmed with immunohistochemistry. Conclusion: Transplanted RPE cells derived from hESC in the defined culture condition successfully survived and migrated within subretinal space of rat retinal degeneration model. These results support the feasibility of the hESC derived RPE cells for cell-based therapies for retinal degenerative disease.

• Journal of Ginseng Research
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• 제47권1호
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• pp.65-73
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• 2023

Background: Age-related macular degeneration (AMD) is a significant visual disease that induces impaired vision and irreversible blindness in the elderly. However, the effects of ginseng berry extract (GBE) on the retina have not been studied. Therefore, this study aimed to investigate the protective effects of GBE on blue light (BL)-induced retinal damage and elucidate its underlying mechanisms in human retinal pigment epithelial cells (ARPE-19 cells) and Balb/c retina. Methods: To investigate the effects and underlying mechanisms of GBE on retinal damage in vitro, we performed cell viability assay, pre-and post-treatment of sample, reactive oxygen species (ROS) assay, quantitative real-time PCR (qRT-PCR), and western immunoblotting using A2E-laden ARPE-19 cells with BL exposure. In addition, Balb/c mice were irradiated with BL to induce retinal degeneration and orally administrated with GBE (50, 100, 200 mg/kg). Using the harvested retina, we performed histological analysis (thickness of retinal layers), qRT-PCR, and western immunoblotting to elucidate the effects and mechanisms of GBE against retinal damage in vivo. Results: GBE significantly inhibited BL-induced cell damage in ARPE-19 cells by activating the SIRT1/PGC-1α pathway, regulating NF-kB translocation, caspase 3 activation, PARP cleavage, expressions of apoptosis-related factors (BAX/BCL-2, LC3-II, and p62), and ROS production. Furthermore, GBE prevented BL-induced retinal degeneration by restoring the thickness of retinal layers and suppressed inflammation and apoptosis via regulation of NF-kB and SIRT1/PGC-1α pathway, cleavage of caspase 3 and PARP, and expressions of apoptosis-related factors in vivo. Conclusions: GBE could be a potential agent to prevent dry AMD and progression to wet AMD.

• 한국임상수의학회지
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• 제14권2호
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• pp.235-237
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• 1997

Cataract and Retinal detachment eyes dogs were examined by ultrasonography. Ultrasonography of the immature and mature cataractous eyes showed echographic changes, and cataractous lens increased in thickness. The diagnosis of retinal detachment was made an echodense waving motion structure in the posterior segment was imaged. This investigation will provide basic information for the study of pathologic conditions affecting the eyes of dog using ultrasound unit commonly available to the veterinarian.