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http://dx.doi.org/10.5653/cerm.2011.38.4.216

Subretinal transplantation of putative retinal pigment epithelial cells derived from human embryonic stem cells in rat retinal degeneration model  

Park, Un-Chul (Department of Ophthalmology, Seoul National University College of Medicine)
Cho, Myung-Soo (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Park, Jung-Hyun (Department of Ophthalmology, Seoul Paik Hospital, Inje University)
Kim, Sang-Jin (Department of Ophthalmology, Seoul National University College of Medicine)
Ku, Seung-Yup (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Choi, Young-Min (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Moon, Shin-Yong (Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University)
Yu, Hyeong-Gon (Department of Ophthalmology, Seoul National University College of Medicine)
Publication Information
Clinical and Experimental Reproductive Medicine / v.38, no.4, 2011 , pp. 216-221 More about this Journal
Abstract
Objective: To differentiate the human embryonic stem cells (hESCs) into the retinal pigment epithelium (RPE) in the defined culture condition and determine its therapeutic potential for the treatment of retinal degenerative diseases. Methods: The embryoid bodies were formed from hESCs and attached on the matrigel coated culture dishes. The neural structures consisting neural precursors were selected and expanded to form rosette structures. The mechanically isolated neural rosettes were differentiated into pigmented cells in the media comprised of N2 and B27. Expression profiles of markers related to RPE development were analyzed by reverse transcription-polymerase chain reaction and immunostaining. Dissociated putative RPE cells ($10^5$ cells/5 ${\mu}L$) were transplanted into the subretinal space of rat retinal degeneration model induced by intravenous sodium iodate injection. Animals were sacrificed at 1, 2, and 4 weeks after transplantation, and immnohistochemistry study was performed to verify the survival of the transplanted cells. Results: The putative RPE cells derived from hESC showed characteristics of the human RPE cells morphologically and expressed molecular markers and associated with RPE fate. Grafted RPE cells were found to survive in the subretinal space up to 4 weeks after transplantation, and the expression of RPE markers was confirmed with immunohistochemistry. Conclusion: Transplanted RPE cells derived from hESC in the defined culture condition successfully survived and migrated within subretinal space of rat retinal degeneration model. These results support the feasibility of the hESC derived RPE cells for cell-based therapies for retinal degenerative disease.
Keywords
Retinal pigment epithelium; Embryonic stem cell; Retinal degeneration; Transplantation; Rat; Human;
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