Embryos of most mammalian species grown in vitro would undergo developmental arrest at the approximate time of genomic activation. Stage-specific cell block and the resulting rapid loss of embryo viability in conventional culture media have limited the duration for which embryos may be cultured prior to transfer. As a result, embryos are usually transferred to the uterus at the 4-to 8-cell stage to avoid the loss of viability associated with long-term in vitro culture. Early transfer has led to asynchrony of the endometrium-trophectoderm interaction at the time of implantation and a resultant reduction in the rate of implantation. To overcome these problems, a variety of co-culture systems has been devised in which embryos can develop for a longer period prior to embryo transfer. Vero cells, derived from African green monkey kidney, share a common embryologic origin with cells from the genital tract. In addition, they are potentially safe to use, since they are highly controlled for viruses and other contaminants. Therefore, co-culture using Vero cells has been widely utilized to enhance embryo viability and development, although not without controversies. We thus designed a series of experiments to demonstrate whether Vero cells do indeed enhance mouse embryo development as well as to compare the efficacy of co-culturing mouse 1-cell embryos on Vero cell monolayer in both Ham's F-10 and human tubal fluid (HTF) culture media. 1-cell stage ICR mouse embryos were cultured either in the presence of Vero cells (Group A) or in conventional culture medium alone (Group B). In Ham's F-10 significantly more 3-to-8cell embryos developed in group A than group B (59.8 versus 10.0%; p<0.01). In contrast, there was no significant difference in embryonic development both group A and group B in HTF. However, significant differences were noted only in later embryonic stage (13 and 0%; p<0.05 of group A and B respectively, hatching or hatched). In Ham's F-10, we also could observe the beneficial effect of Vero cell on hatching process (70.7 and 42.1%; p<0.05 of group A and group B respectively).
Journal of Korean Society of Archives and Records Management
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v.2
no.2
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pp.107-135
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2002
This study concerns Sama Pangmok which is in the possession of Harvard-Yenching Library, Harvard University, USA. Sama Pangmok is the archive of Saengwon Chinsa Examination(the licentiate examination) which is the preliminary test for main civil service examination. And it is the list of the names of the applicants who passed this examination. This examination were conducted from the days of lauching of Chosun as a new nation to the days of Kabo Kyungjang(the 1894 reform). While this examination was conducted 230 times, not all but 186 of the resultant lists are available. Of these 186 lists, this library posses 47 titles. My study of the existing dissertation on the possession of titles by Harvard-Yenching Library, 7 titles where in possession of the same library in 1968 and 5 ones in 190. It is the main objective of this study to clarify the possession of this library and correct the past errors in indication of possession of titles by this library. For this purpose, I conducted a study on Saengwon Chinsa Examination and Sama Pangmok, which could be the historical background of this study. This was followed by research on the bibliographical aspect of Sama Pangmok and the research on the situation concerning this Library's possession of the same lists. Lastly, the analysis of the special feature of Sama Pangmok possessed by this Library was conducted. It is expected that the result of this research will be applied to the practical research of the history of civil service examinations and the old and rare books overseas in the future.
A $1{\times}16$ thermo-optic switch with small excess loss using multimode interference(MMI) couplers is designed, fabricated, and measured. This paper introduces the proposed $1{\times}16$ thermo-optic switch, and discusses the measurement results. The $1{\times}16$ thermo-optic switch is farmed as 4-stage which consists of 15 unit devices. The unit devices are the $2{\times}2$ thermo-optic switches with Mach-Zehnder interferometer(MZI) structure. The characteristics of the $1{\times}16$ thermo-optic switch depends strongly on each unit device. The unit deviceconsists of two 3-dB general interference MMI couplers and two single mode waveguide arms as a phase shifter. First of all, the 3-dB optical splitter and $2{\times}2$ MZI thermo-optic switch have been tested to confirm the characteristics of the unit devices of the $1{\times}16$ MZI thermo-optic switch. Using the measurement results of the unit devices, the $1{\times}16$ MZI thermo-optic switch can be produced with better characteristics. The resultant structure of the MMI coupler with the optical light source of wavelength of 1550nm for the $1{\times}16$ thermo-optic switch is that the width and the optimized length are $25{\mu}m\;and\;1580{\mu}m$, respectively. The smallest excess loss fur the unit device is -0.5dB and the average excess loss is -0.7dB.
Journal of the Korean Society of Marine Environment & Safety
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v.27
no.5
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pp.691-699
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2021
The global surface temperature has risen critically over the past century and according to the IPCC Fifth Assessment Report 2014, existing risks in natural and human systems will worsen. Coastal erosion is mostly caused by climate change and among all the coastal areas at risk, Benin, which is part of the Gulf of Guinea, has been ranked very highly as a vulnerable region. Therefore, in this review, we focus on the evolution of coastline change in Cotonou of Benin, summarizing its resultant impacts and applied measures around the coast area by reviewing previous studies. Signs of coastal erosion in Cotonou appeared in 1963. After 39 years, the east shoreline of Cotonou has retreated by 885 m, resulting in the disappearance of more than 800 houses. To solve this problem, Benin authorities built seven groynes in 2013, and have increased the number of the structure as a way to interrupt water flow and limit the movement of sediment. Over the region, shorelines appeared preserved accordingly. In contrast, areas located further east, where groynes were not installed, have suf ered from intensive erosion at a rate of 49 m/yr. In the future, as a next step, the effectiveness of groynes should be studied with local and broader perspectives.
Xue, Ge;Cheng, Silu;Yin, Jingwen;Zhang, Runxiang;Su, Yingying;Li, Xiang;Li, Jianhong;Bao, Jun
Animal Bioscience
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v.34
no.6
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pp.1070-1077
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2021
Objective: An experiment was conducted to determine the appropriate fasting time prior to slaughter for broilers in floor-feed and scatter-feed mode. Methods: On 21 d since hatching, 120 Arbor Acres broilers were divided into floor-feed and scatter-feed groups, chicks from each group were further assigned to feed withdrawal treatments for 0, 4, 6, 8, and 10 h. Some resultant indicators such as carcass contamination, body weight loss, meat quality of 54-day-old broilers were measured. Results: It appears that longer feed withdrawal increased weight loss, lightness, drop loss of meat but reduced pH. A significant higher weight loss and lightness for both floor-feed and scatter-feed chicks coincided after 6 to 10 h feed withdrawal (p<0.05). pH for breast muscle at 45 min postmortem reduced when chicks of scatter-feed were fasted 6 and 10 h, while the reduction of floor-feed group occurred only in 10 h (p<0.05). A noticeable effect of feed withdrawal on drop loss occurred after 10 h fasting in scatter-feed of which drop loss were significantly higher than that for other groups including control (p<0.05). The change of contamination propensity revealed that 6 to 10 h fasting significantly reduced the likelihood of carcass contamination under both floor-feed and scatter-feed (p<0.05). Net weights of intestinal contents for gizzard were significantly reduced after feed deprived for 10 h in floor-feed and 6 and 10 h in scatter-feed (p<0.05). The decrease for whole intestine occurred after floor-feed broilers have been without feed for more than 4 h, scatter-feed broilers for more than 8 h (p<0.05). Conclusion: On the premise that poultry product properties and welfare were not significantly damaged, proper fasting time could reduce carcass contamination. Current data implied that 6 h fasting was recommendable for both floor and scatter feed pre-slaughter broilers.
This study analyzed the expected economic effects of the Korea-GCC FTA and sought strategies for industrial cooperation. To see the economic effects of Korea-GCC FTA, we analysed the effect of the oil tariff reduction of economy by Vector Autoregression(VAR) model. The estimation results shows that following the abolishment of the tariff on crude oil imports, GDP, GNI and consumption are expected to grow by 0.212%, 0.389% and 0.238%, respectively. Meanwhile, investment, export and import are estimated to drop by 0.462%, 0.413% and 0.342%, respectively. As for prices, producer prices are to rise by 6.356%p, whereas consumer prices fall by 2.996%p. In short, the Korea-GCC FTA and resultant abolishment of the tariff on crude oil imports followed by the decline in crude oil prices will result in declining prices whilst macroeconomic indices, such as GDP, GNI and consumption, will increase exerting positive effects on domestic economic growth. Also, it is necessary to proactively respond to GCC member states' industrial diversification policies for FTA-based industrial cooperation to diversify the sources of crude oil and natural gas imports for further resource risk management.
The combination of animals has been developed in each of the cultural spheres as a method of metaphor and symbolism of the cultural code. However, its symbolism is not a fixed constant, but a variable and relative constant. This work focused on its features in comparison with East Asian cultural spheres comedic stories and proverbs. Consequently, several features were identified. First, the combinations of animals in similar comedic stories and proverbs among Korea, Japan and China show a difference in point of view. Korean focuses on the difference of the two animals, but Chinese and Japanese focus on the differences in value and level. Second, the method of anthropomorphization is relatively more developed in China and Japan than Korea. The combinations of animals of Chinese comedic stories and proverbs particularly in the field of anthropomorphization, are most focused on age and sex of the animal. The animal's age or sex remains mostly undetermined in Korean animal's proverbs, unlike Chinese proverbs. On the other hand, two animals in Japanese comedic stories and proverbs are usually of the male and female gender from. Third, the combinations of animals of Chinese and Japanese focus on the animal's body and its characteristics of action. Chinese and Japanese combine the characteristics of the two animal's bodies and actions. This feature apparently caused the resultant combinations of the animal's body parts, for example, the Dragon. Understanding of the combinations of two animals is a good portal into the features of East Asian culture sphere.
Park, Sang-Mo;Park, Ji-Man;Kim, Seong-Kyun;Heo, Seong-Joo;Koak, Jai-Young
Journal of Korean Dental Science
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v.12
no.1
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pp.13-19
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2019
Purpose: The aim of this study was to compare the flexural strength of provisional fixed dental prostheses which was three-dimensional (3D) printed by several build directions. Materials and Methods: A metal jig with two abutment teeth and pontic space in the middle was fabricated. This jig was scanned with a desktop scanner and provisional restoration was designed on dental computer-aided design program. On the preprocessing software, the build angles of the restorations were arranged at $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ and support was added and resultant structure was sliced to a thickness of $100{\mu}m$. Processed restorations were printed with digital light processing type 3D printer using poly methyl meta acrylate-based resin. After washing and post-curing, compressive loading was applied at a speed of 1 mm/min on a metal jig fixed to a universal testing machine. The maximum pressure at which fracture occurred was measured. For the statistical analysis, build direction was set as the independent variable and fracture strength as the dependent variable. One-way analysis of variance and Tukey's post hoc analysis was conducted to compare fracture strength among groups (${\alpha}=0.05$). Result: The mean flexural strength of provisional restoration 3D printed with the build direction of $0^{\circ}$ was $1,053{\pm}168N$; it was $1,183{\pm}188N$ at $30^{\circ}$, $1,178{\pm}81N$ at $45^{\circ}$, $1,166{\pm}133N$ at $60^{\circ}$, and $949{\pm}170N$ at $90^{\circ}$. The group with a build direction of $90^{\circ}$ showed significantly lower flexural strength than other groups (P<0.05). The flexural strength was significantly higher when the build direction was $30^{\circ}$ than when it was $90^{\circ}$ (P<0.01). Conclusion: Among the build directions $0^{\circ}$, $30^{\circ}$, $45^{\circ}$, $60^{\circ}$, and $90^{\circ}$ set for 3D printing of fixed dental prosthesis, an orientation of $30^{\circ}$ is recommended as an effective build direction for 3D printing.
Kim, Ji-Su;Jung, Ji young;Ha, Si Young;Yang, Jae-Kyung
Journal of the Korean Wood Science and Technology
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v.47
no.4
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pp.393-407
/
2019
In this study, we prepared lignocellulosic artificial soil that contains Bacillus subtilis (peat moss/perlite/ steam-exploded oak wood/microbial culture = 3:1:3:3, w/w/w/w) for use in the restoration of damaged soil areas. The prepared lignocellulosic artificial soil was mixed with soil at ratios of 0%, 25%, 50%, 75%, and 100%. These mixed soils were then applied to fields, and the resultant physicochemical properties and their effects on the plant growth of Lespedeza cyrtobotrya were observed. The mixture of the prepared artificial soils (mixed at ratios of 25%-100%) with soil had a bulk densities of <$0.04g/cm^3$, porosities of >85%, pH values between 4.3 and 4.7, electrical conductivities of <0.5 dS/m, C/N ratios between 15.0 and 26.5, organic matter content between 23.6% and 43.2%, and bacterial densities between $157{\times}10^6$ and $624{\times}10^6CFU/g$. In addition, the prepared artificial soils mixed with soil at ratios of 25%-50% exhibited higher plant growth rates for L. cyrtobotrya compared with the control. Overall, we identified positive correlations between the plant growth of L. cyrtobotrya and soil bulk density, porosity, water-holding capacity, C/N ratio, organic matter, and bacterial densities.
Kim, Wonkook;Roh, Sang-Hyun;Moon, Yongseon;Jung, Sunghun
Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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v.37
no.3
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pp.167-175
/
2019
Water color analysis allows non-destructive estimation of abundance of optically active water constituents in the water body. Recently, there have been increasing needs for light-weighted multispectral cameras that can be integrated with low altitude unmanned platforms such as drones, autonomous vehicles, and heli-kites, for the water color analysis by spectroradiometers. This study performs the preprocessing of the Micasense Rededge-M camera which recently receives a growing attention from the earth observation community for its handiness and applicability for local environment monitoring, and investigates the applicability of Rededge-M data for water color analysis. The Vignette correction and the band alignment were conducted for the radiometric image data from Rededge-M, and the sky, water, and solar radiation essential for the water color analysis, and the resultant remote sensing reflectance were validated with an independent hyperspectral instrument, TriOS RAMSES. The experiment shows that Rededge-M generally satisfies the basic performance criteria for water color analysis, although noticeable differences are observed in the blue (475 nm) and the near-infrared (840 nm) band compared with RAMSES.
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