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Contribution of Different Types of $Ca^{2+}$ channels to Catecholamine Secretion in Rat Adrenal Chromaffin Cells (부신수질 Chromaffin 세포의 $Ca^{2+}$ 통로유형이 카테콜아민 분비에 미치는 영향에 관한 정량적 연구)

  • Goo, Yang-Soak;Roh, Jin-A;Lee, Jung-Hwa;Chao, Eun-Jong
    • Progress in Medical Physics
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    • v.8 no.1
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    • pp.3-15
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    • 1997
  • Adrenal chromaffin cells secrete catecholamine in response to acetylcholine. The secretory response has absolute requirement for extracellular calcium, indication that $Ca^{2+}$ influx through voltage dependent $Ca^{2+}$ channel (VDCC) is the primary trigger of the secretion cascade. Although the existence of various types of $Ca^{2+}$ channels has been explored using patch clamp technique in adrenal chromaffin cells, the contribution of different types of $Ca^{2+}$ channels to catecholamine secretion remains to be established. To investigate the quantative contribution of different types of $Ca^{2+}$ channels to cate-cholamine secretion, $Ca^{2+}$ current($I_{Ca}$) and the resultant membrane capacitance increment($\Delta{C}_{m}$) were simultaneoulsy measured. Software based phasor detector technique was used to monitor $\Delta{C}_{m}$. After blockade of L type VDCC with nicardipine (1$\mu$M), $I_{ca}$ was blocked to 43.85$\pm$6.72%(mean$\pm$SEM) of control and the resultant ㅿC$_{m}$ was reduced ot 30.10$\pm$16.44% of control. In the presence of nicardipine and $\omega$-conotoxin in GVIA(l$\mu$M), an N type VDCC antagonist, $I_{ca}$ was blocked to 11.62$\pm$2.96% of control and the resultant $\Delta{C}_{m}$ was reduced to 26.13$\pm$8.25% of control. Finally, in the presence of L, N, and P type $Ca^{2\pm}$ channel antagonists(nicardipine, $\omega$-Conotoxin GVIA, and $\omega$-agatoxin IVA, respectively), $I_{ca}$ and resultant $\Delta{C}_{m}$ were almost completely blocked. From the observation of parallel effects of $Ca^{2+}$ channel antagonists on $I_{ca}$ and $\Delta{C}_{m}$, it was concluded that L, N, and also P type $Ca^{2+}$ channels served and $Ca^{2+}$ source for exocytosis and no difference was observed in their efficiency to evoke exocytosis amost L, N, and P type $Ca^{2+}$ channels.

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A Kinetic Analysis of the Lower Extremity during Walking on Three Different Stair width in Healthy Adults (성인 계단보행 시 계단 너비에 따른 하지의 운동역학적 분석)

  • Jun, Hyun-Min;Ryu, Ji-Seon
    • Korean Journal of Applied Biomechanics
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    • v.18 no.4
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    • pp.161-169
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    • 2008
  • The purpose of this study was to investigate kinetic variables of the lower extremity during walking on three different stair widths in healthy adults. Ten healthy college-aged adults($23.5{\pm}3.5$) recruited for this study. Each stairs with the same height and length(l8cm and 90cm) under the Korean Constructional Law but three different widths(26cm, 31cm and 36cm) were conducted for this study. One force plate(9286AA, Kistler Co.) was put on third stairs. One-way ANOVA was performed to find the stair width effects during stair walking and the following findings ware obtained. There was significantly decreased in ankle resultant joint moment at Pull-Up phase(p<.05) and, significantly increased in knee extension moment during mid-stance phase as stair width increase(p<.05), but there was no significance in ankle resultant joint moment was found at Forward Continuance Phase in Ascending Stair Walking and There was significantly increased in ankle resultant joint moment as stair width increase during mid-stance phase(p<.05) and no significance in knee and hip resultant moments among the stair width in descending stair walking.

A knowledge base construction and an application to control (지식베이스 구축과 제어응용)

  • 김도성;이명호
    • 제어로봇시스템학회:학술대회논문집
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    • 1989.10a
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    • pp.407-412
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    • 1989
  • Using the knowledge base which contains the patterns and data of the past experience of a plant, a learning control method is suggested. The knowledge for controlling a plant is stored to the knowledge base and continually modified after performance evaluation of an applied control input. The performance of the resultant knowledge based control system is examined by an application to process.

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Design of High-Speed 2-D State-Space Digital Filters Based on a Improved Branch-and-Bound Algorithm (개량된 분기한정법에 의한 고속연산 2차원 상태공간 디지털필터의 설계)

  • Lee Young-Ho
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.10 no.7
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    • pp.1188-1195
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    • 2006
  • This paper presents an efficient design method of 2-D state-space digital filter based on an improved branch-and -bound algorithm. The resultant 2-D state-space digital filters whose coefficients are represented as the sum of two power-of-two terms, are attractive for high-speed operation and simple implementation. The feasibility of the proposed method is demonstrated by several experiments. The results show that the approximation error and group delay characteristic of the resultant filters are similar to those of the digital filters which designed in the continuous coefficient space.

Development of Eco-friendly Woven Floor Mat with High Resilience II - Characterization of TPU Coating Yarn and Floor Mat - (고탄성 특성을 보유한 친환경 우븐 바닥재에 관한 연구(II) - TPU 코팅사 및 바닥재의 특성-)

  • Lee, Sun-Hee
    • Fashion & Textile Research Journal
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    • v.14 no.4
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    • pp.635-640
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    • 2012
  • In this study, thermoplastic urethane (TPU) coating yarns were prepared at various extruding temperatures. The fine structure and mechanical properties of resultant TPU coating yarns examined by the wide angle X-ray diffractometer (WAXD), differential scanning calorimetry (DSC), dynamic mechanical thermal analysis (DMTA), and tensile test. TPU coating yarns (prepared at extruding temperatures at $175^{\circ}C$) were confirmed as a stable fine structure that obtained excellent tensile strength and flexibility. The C samples prepared by optimized conditions made by TPU woven floor mat. The structure of the woven mat is $4{\times}4$ basket weave and have laminated with the EVA foam to obtained final TPU woven floor mat products. The resultant TPU woven floor mat was obtained to 1.5MN of tensile strength, 22% of the elongation, and 0.2MN of tear strength. The weight loss abrasion and the resilience by the ball rebound of the TPU-woven floor mat was prior to those of the PVC subsequently, we were able to develop a woven floor mat with TPU coating yarn and produce an eco-friendly high valuable woven floor mat using an interior product.

Effect of addition of methanol on rheological properties of silk formic acid solution

  • Bae, Yu Jeong;Um, In Chul
    • International Journal of Industrial Entomology and Biomaterials
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    • v.40 no.1
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    • pp.28-32
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    • 2020
  • Recently, many studies have been undertaken on the wet spinning and electrospinning of silk because wet-spun fibers and electrospun webs of silk can be applied in the biomedical and cosmetic fields owing to the good biocompatibility of silk. The rheological properties of silk solution are important because they strongly affect the spinning performance of the silk solution and the structures of resultant fibrous materials. Therefore, as a preliminary study on the effect of solvent composition on the rheological properties of silk fibroin (SF) solution and structure of the resultant film, in the reported work, methanol was added to the SF formic acid solution. A small amount of methanol (i.e. 2%) added to the SF formic acid solution significantly altered the rheological properties of the solution: its shear viscosity increased by 10 folds at low shear and decreased on increasing the shear rate, demonstrating shear thinning behavior of the SF solution. Dynamic tests for the SF solution indicated that the addition of 2% methanol altered the viscous state of the SF formic acid solution to elastic. However, the molecular conformation (i.e. β-sheet conformation) of the regenerated SF film cast from formic acid remained unchanged on the addition of 2% methanol.

Study on Fatigue Behavior of Carbon Fiber Reinforced Polyimide Composites (탄소섬유강화 복합적층판의 피로특성에 관한 연구)

  • 이창수;황운봉;한경섭;윤병일
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.15 no.1
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    • pp.49-60
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    • 1991
  • Fatigue behavior of carbon fiber reinforced polyimide composite materials was studied experimentally and analytically. The physical variables, such as cyclic displacements and hysteresis loop energy were observed during fatigue tests. Fatigue life of the investigated [0/90]$_{2S}$ laminates was predicted by H'||'&'||'H models which was proposed based on the fatigue modulus and resultant strain. The predicted fatigue life by H'||'&'||'H curves was reasonably close to the experimental data. Fractography study shows that fatigue failure mechanism of [0/90]$_{2S}$ laminated composite materials involves failure break, matrix tearing and fiber-matrix debonding as well as delamination of layers.