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Expression pattern of prohibitin, capping actin protein of muscle Z-line beta subunit and tektin-2 gene in Murrah buffalo sperm and its relationship with sperm motility

  • Xiong, Zhaocheng;Zhang, Haihang;Huang, Ben;Liu, Qingyou;Wang, Yingqun;Shi, Deshun;Li, Xiangping
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.11
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    • pp.1729-1737
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    • 2018
  • Objective: The aim of the current study is to investigate the relationship between prohibitin (PHB), capping actin protein of muscle Z-line beta subunit (CAPZB), and tektin-2 (TEKT2) and sperm motility in Murrah buffalo. Methods: We collected the high-motility and low-motility semen samples, testis, ovary, muscle, kidney, liver, brain and pituitary from Murrah buffalo, and analysed the expression of PHB, CAPZB, and TEKT2 in mRNA (message RNA) and protein level. Results: Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) result showed that the expression of PHB was higher and CAPZB, TEKT2 were specifically expressed in testis as compared to the other 6 tissues, and that in testis, the expression of TEKT2 was higher than that of CAPZB and PHB. Immunohistochemistry test revealed that all three genes were located on the convoluted seminiferous tubule and enriched in spermatogenic cells. Both qRT-PCR and Western Blot results showed that the expression levels of PHB, CAPZB, and TEKT2 were significantly lower in the low-motility semen group compared to the high-motility semen group (p<0.05). Conclusion: The expression levels of PHB, CAPZB, and TEKT2 in Murrah buffalo sperm have a high positive correlation with sperm motility. And the three genes may be potential molecular markers for the decline of buffalo sperm motility.

Heritage Soft Power in East Asia's Memory Contests: Promoting and Objecting to Dissonant Heritage in UNESCO

  • Nakano, Ryoko
    • Journal of Contemporary Eastern Asia
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    • v.17 no.1
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    • pp.50-67
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    • 2018
  • Heritage has entered the center stage of public diplomacy in East Asia. Competition to claim and interpret memories of World War II in East Asia has driven campaigns to list heritage items with UNESCO. State and non-state actors aim to use heritage listings to present a particular view of the war and related history to domestic and international audiences. This paper highlights the role of heritage soft power in East Asia's "memory contests" by examining the promotion of dissonant modern heritage in UNESCO's heritage programs. It conceptualizes heritage designation as a soft power resource in East Asia and presents a conceptual framework for understanding the hegemonic competition over the "memory regime" that emerged from the structural change in East Asia's regional order. It then uses this framework to analyze the processes by which state and non-state actors promote and/or object to UNESCO recognition of their sites and documents as heritage of outstanding universal value or world significance. The elements of this process are illustrated with case studies of two very different pieces of heritage, Japan's "Sites of the Meiji Industrial Revolution" and China's "Documents of Nanjing Massacre," which were enshrined as significant world heritage in 2015. While state and non-state actors in East Asia are increasingly recognizing the utility of heritage as a soft power resource for advancing specific historical narratives to an international audience, a backlash movement from civil society groups and governments in other countries prevents a purely unilateral interpretation. As a result, the utility of heritage soft power in this context must be significantly qualified.

A Quantitative Evaluation and Comparison of Harmonic Elimination Algorithms Based on Moving Average Filter and Delayed Signal Cancellation in Phase Synchronization Applications

  • Xiong, Liansong;Zhuo, Fang;Wang, Feng;Liu, Xiaokang;Zhu, Minghua;Yi, Hao
    • Journal of Power Electronics
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    • v.16 no.2
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    • pp.717-730
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    • 2016
  • The harmonic components of grid voltage result in oscillations of the calculated phase obtained via phase synchronization. This affects the security and stability of grid-connected converters. Moving average filter, delayed signal cancellation and their related harmonic elimination algorithms are major methods for such issues. However, all of the existing methods have their limitations in dealing with multiple harmonics issues. Furthermore, few studies have focused on a comparison and evaluation of these algorithms to achieve optimal algorithm selections in specific applications. In this paper, these algorithms are quantitatively analyzed based on the derived mathematical models. Moreover, an enhanced moving average filter and enhanced delayed signal cancellation algorithms, which are applicable for eliminating a group of selective harmonics with only one calculation block, are proposed. On this basis, both a comprehensive comparison and a quantitative evaluation of all of the aforementioned algorithms are made from several aspects, including response speed, required data storage size, sensitivity to sampling frequency, and elimination of random noise and harmonics. With the conclusions derived in this paper, better overall performance in terms of harmonic elimination can be achieved. In addition, experimental results under different conditions demonstrate the validity of this study.

Characteristic of Hydrogen Generation from Solid-State NaBH4 and Fuel Cell Operation for Fuel Cell Aircraft (연료전지 항공기를 위한 고체상태 NaBH4의 수소발생 및 연료전지 구동 특성)

  • Lee, Chung-Jun;Kim, Tae-Gyu
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.39 no.9
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    • pp.858-865
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    • 2011
  • This paper describes the characteristics of hydrogen generation from solid-state $NaBH_4$ and fuel cell operation for fuel cell aircraft. The solid-state $NaBH_4$ was used for a high hydrogen storage density, and was reacted with hydrochloric acid to generate hydrogen. The hydrogen generation rate for the solid-state $NaBH_4$ reaction was measured at various conditions. As a result, the hydrogen generation rate was increased with the feed rate and concentration of hydrochloric acid, while not be affected by the reaction temperature. A fuel cell was connected with the solid-state $NaBH_4$ hydrogen generator. The stable power output was obtained at the gradual and sudden increases of electric loads.

Energy Transfer Pathway in Luminescent Lanthanide Complexes Based on Dansyl-N-methylaminobenzoic Acid through Intramolecular Charge Transfer State for Near Infrared Emission

  • Roh, Soo-Gyun;Baek, Nam-Seob;Kim, Yong-Hee;Kim, Hwan-Kyu
    • Bulletin of the Korean Chemical Society
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    • v.28 no.8
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    • pp.1249-1255
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    • 2007
  • We have investigated the photophysical properties of dansyl-N-methylaminobenzoic acid (DABAH) as a ligand and its lanthanide (Ln3+)-cored complexes (Ln3+-(DABA)3(terpy)) in order to determine the main energy transfer pathway for sensitized near infrared emission of Ln3+ ions (Ln3+ = Nd3+ and Er3+) in Ln3+- (DABA)3(terpy). The fluorescence spectrum of DABAH shows a large Stokes shift with increasing solvent polarity. This large Stokes shift might be due to the formation of a twisted intramolecular charge transfer (TICT) state, as demonstrated by the large dipole moment in the excited state. It is in good agreement with the result that the phosphorescence even in the Gd3+-cored complex based on the DABAH ligand was not observed, maybe due to the highly forbidden character of the S1 → T1 transition in the DABAH ligand. A short decay component (ca. 1 ns) was observed in Er3+-(DABA)3(terpy) whereas the fluorescence lifetimes of DABAH and its Gd3+-(DABA)3(terpy) are observed about ~10 ns. The short component could be originated from the energy transfer process between the ligand and the Ln3+ ion. Based on the fluorescence of DABAH its Ln3+- (DABA)3(terpy), the sensitization of Ln3+ luminescence in the Ln3+-(DABA)3(terpy) takes place by the energy transfer via the TICT state of DABAH in the excited singlet state rather than via the excited triplet state.

Smoking Behavior among Industrial Workers In Jecheon (산업체 근로자의 흡연행태에 관한 연구)

  • Kim Myoung Sook;Kim Myoung Hee
    • Journal of Korean Public Health Nursing
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    • v.19 no.1
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    • pp.18-29
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    • 2005
  • The purpose of this study was to analyze the smoking behavior and to provide the basic data required to develop a smoking cessation program. The study subjects were 407 industrial workers in Jecheon. The data were collected using a structured questionnaire. The data were analyzed by the SPSS 10.0 system using descriptive statistics, t-test, and Chi-square test. The study results were as follows 1. Current smokers were $64.0\%$, ex-smokers were $19.1\%$. and non-smokers were $16.9\%$. 2. The most common motives of smoking were anger. anxiety, depression. and stress in the workplace. 3. In smoking habits, about $65.1\%$ of the current smokers smoked 11-20 cigarettes/day, and $91.0\%$ had smoked for more than 5 years. The smoking areas of the workplace were outdoor $37.9\%,\;rest\;room\;34.2\%,\; lobby\;23.0\%,\;corridor\;21.4\%,\;and\;office\;8.6\%$. 4. Regarding smoking cessation, $78.2\%$ of current smokers had attempted. The frequency of smoking cessation attempts was 1-3 or less in $67.2\%$ of smokers. The duration of smoking cessation was 1-3 month or less in $61.6\%$ of the smokers. 5. The mean score for smoking knowledge was 17.62, smoking attitude 75.74. and perceived health state 12.27. 6. There were significant differences between smokers and non-smokers 10 smoking attitude (t=5.29, p=0.00), and perceived health state(t=6.47, p=0.00). 7. As a result of the homogeneity test, both types of smoking and perceived health state proved to have significant differences in the change of health state compared with 4 weeks ago'$(x^2=20.26,\;p=0.00)$ and the level of satisfaction of health state in activities of daily living'$(x^2=15.47,\; p=0.05)$. The important findings of this study showed that a smoking cessation program needs to be developed to enhance the negative smoking attitude. to promote the perceived health state through smoking cessation, to encourage the intention of smoking cessation, and to develop strategies of stress management.

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The Responsibility of the State for Financing of the National Health Insurance (의료보험 재정에서의 국가 책임)

  • Lee, Jun-Young
    • Korean Journal of Social Welfare
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    • v.57 no.4
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    • pp.321-342
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    • 2005
  • The finance of the National Health Insurance(NHI) in nearly every Nation in the world has been traditionally based on premiums of the workers and employers. But in Korea, the government has been guaranteeing financial supports to regional health corporations. After the unification of the different corporations in the NHIC, the government will not have to give financial support to the NHIC. Then this will be a serious financial challenge to NHIC, which has usually had financial deficit. The purpose of this paper is to review the problems of the premium based financing of the NHI and to exam whether such problems will be solved through the financial support from the state to the NHI. The analysis in this paper focused on five viewpoints; 1) work relatedness 2) redistribution effect 3) financial burden of business firms through the premium 4) risk pooling 5) management hegemony of the NHI. As a result, it was found that there are many problems in every five aspects and these problems could be solve through the financial aid from the state. But, it does not without any restriction mean to suggest that the financing mode of the korean NHI should be wholly transformed to a tax based financing mode. Because there are many things to be considered in oder to alter the financing mode of the NHI. Nevertheless, this paper would give a logical background to enlarge the financial aid form the state to NHI, or at least, to maintain it at a present level.

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In-transit development of color abnormalities in turkey breast meat during winter season

  • Carvalho, Rafael H.;Honorato, Danielle C.B.;Guarnieri, Paulo D.;Soares, Adriana L.;Pedrao, Mayka R.;Oba, Alexandre;Paiao, Fernanda G.;Ida, Elza I.;Shimokomaki, Massami
    • Journal of Animal Science and Technology
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    • v.60 no.1
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    • pp.30.1-30.10
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    • 2018
  • Background: The poultry industry suffers losses from problems as pale, soft and exudative (PSE), and dark, firm and dry (DFD) meat can develop in meat as a result of short- and long-term stress, respectively. These abnormalities are impacted by pre-slaughter animal welfare. Methods: This work evaluated the effects of open vehicle container microclimate, throughout the $38{\pm}10km$ journey from the farm to the slaughterhouse, on commercially turkey transported during the Brazilian winter season. The journey was initiated immediately after water bath in truck fitted with portable Kestrel anemometers to measure air ventilation, relative humidity, temperature and ventilation. Results: The inferior compartments of the middle and rear truck regions showed highest temperature and relative humidity, and lower air ventilation. In addition, the superior compartments of the front truck regions presented lower temperature and wind chill, and highest air ventilation. The breast meat samples from animals located at the inferior compartments of the middle and rear truck regions and subjected to with water bath (WiB) treatment presented highest DFD-like and had lowest PSE-like meat incidence than those from animals located at other compartments within the container. Lower incidence of PSE-like meat was observed in birds without water bath (WoB). Conclusions: Assessment on turkeys transported under Brazilian southern winter conditions revealed that breast meat quality can be affected by relative humidity, air ventilation, temperature, and transport under subtropical conditions promoting color abnormalities and the formation of simultaneously PSE-like and DFD-like meat.

Specifics of Speech Development of Children with Cerebral Palsy

  • Zavitrenko, Dolores;Rizhniak, Renat;Snisarenko, Iryna;Pasichnyk, Natalia;Babenko, Tetyana;Berezenko, Natalia
    • International Journal of Computer Science & Network Security
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    • v.22 no.11
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    • pp.157-162
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    • 2022
  • Cerebral palsy is one of the most serious forms of disorders of the psychophysical development of children, which manifests itself in disturbances of motor functions, which are often combined with speech disorders, other complications of the formation of higher mental functions, and often with a decrease in intelligence. The article will discuss the speech disorder in children with cerebral palsy. Emphasis is placed on some important aspects, which should bear in mind, investigating the problem of specifics of speech development of children with cerebral palsy. In particular at the heart of speech disorders in the cerebral palsy is not only damage to certain structures of the brain, but also the later formation or underdevelopment of those parts of the cerebral cortex, which are of major importance in linguistic and mental activity. This is an ontogenetically young region of the cerebral cortex, which is most rapidly developing after birth (premotor, frontal, temmono-temporal). It is important to take into account, that children with cerebral palsy have disturbances of phonemic perception. Often, children do not distinguish between hearing sounds, cannot repeat component rows, allocate sounds in words. At dysarthria, there are violations of pronunciation of vowel and consonant sounds, tempo of speech, modulation of voice, breathing, phonation, as well as asynchronous breathing, alignment and articulation. As a result, we identified the main features and specifics of the speech development of children with cerebral palsy and described the conditions necessary for the full development of language. Language disturbances in children's cerebral palsy depend on the localization and severity of brain damage. Great importance in the mechanism of speech disorders has a pathology that limits the ability of movement and knowledge of the world.

The Trajectories of Welfare States after Global Economic Crisis (세계 경제위기 이후 복지국가의 진로)

  • Joo, Eunsun
    • Korean Journal of Social Welfare Studies
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    • v.42 no.2
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    • pp.97-122
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    • 2011
  • After global economic crisis, most countries increased the welfare expenditure as a part of stimulus package. As a However welfare expenditure was decreased radically as the crisis was transmitted into the financial crisis. Which turning point is the welfare state going through now? Although the need for the welfare and the role of the state to take responsibility of public welfare has increased because of poverty and polarization, responses of the state against the crisis had focused on the aid to the financial industry and cutting taxes and showed limitations in coordination. Financial limitation of welfare expenditure, political individualism, the change of class politics and the mixture of the welfare institutions and financial institutions make have a pessimistic prospect of the retrenchment to the minimalist welfare state. As neoliberal state is continued cash benefits mainly for the middle class is being decreased. As a result, the direction the welfare states pursue is prospected to win over the poor by strengthening selectivity in welfare provision rather than class coordination.