• 생태와환경
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• 제41권1호
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• pp.81-89
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• 2008

우리나라 연안의 저서성 요각류 Tigriopus japonicus s.l.를 대상으로 내분비계 교란물질인 4-nonylphenol에 대한 생태독성 반응을 연구한 결과, 4NP는 $30{\mu}g\;L^{-1}$이하에서는 농도에 관계없이 생존율에는 영향을 주지 않았으며, 포란율, 부화율 등 생식 능력의 저하 현상 역시 찾아 볼 수 없었다. 그러나 노출된 T. japonicus s.l. nauplius 유생과 copepodite 유생은 모든 농도에서 대조군보다 성장이 지연되는 것으로 나타났으며, 처리 농도가 높아질수록 개체의 크기와 생체량이 줄어드는 것으로 나타났다. 또한 DEG를 이용한 유전자 발현의 차이를 확인하여 biomarker로서 가능성을 지닌 유전자를 확인해 볼 수 있었다. 추후 형태학적 연구와 분자생물학적인 연구를 계속 진행하여 생태계 평가를 할 수 있는 실질적인 생체지표 개발을 할 수 있을 것으로 생각된다.

• 산업경영시스템학회지
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• 제24권65호
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• pp.51-63
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• 2001

The present experiment investigated the possibility of evaluating of the human sensibility contingent on the speed and modes of driving using the responses from the autonomic nervous system, subjective assessments, Simulator Sickness (55) in dynamic simulator. The three conditions of the speed of driving were 40 $\pm$ 10 km/h, 100$\pm$10 km/h, 160 $\pm$ 10 km/h, and the participant was instructed to drive the car for three minutes on the elliptical track. It is programed in such a way that the modes of driving can be changed smoothly using road DB in Dynamic Simulator, and for signifying the change of the condition the road signs were used. The instruction was given to the participant to drive the car on the fixed speed of 20 km/h for 30 seconds, then to drive the car on sudden-start mode of driving from the 20 km/h to 160 km/h within 10 seconds. For the sudden-stop mode of driving, it was instructed that stop the car from the speed of 160km/h to 20km/h within 10 seconds when the subject see the road sign, then drive the car at the fixed speed of 20 km/h for 30 seconds. The results of the subjcetive assessment showed that the level of pleasantness and the tension was increased, and physiological response showed that the level of activity of the autonomic responses were also increased as the speed of the car increased. Also, for results on the driving modes showed that the level of pleasantness was highest for the sudden-stop, next highest was sudden-start, and the lowest was 20 km/h fixed speed condition for the subjective assessment, and tile order of the level of activation of the autonomic nervous system showed the same results as above. From the results of the present study it was concluded that the presentation and evaluation of the stimulus for the human sensitivity is possible in dynamic simulator.

• Clinical and Experimental Reproductive Medicine
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• 제48권3호
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• pp.221-228
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• 2021

Objective: This study investigated the effect of crocin in methylglyoxal (MGO)-induced diabetic male mice. Methods: Seventy 1-month-old male NMRI mice weighing 20-25 g were divided into seven groups (n=10): sham, MGO (600 mg/kg/day), MGO+crocin (15, 30, and 60 mg/kg/day), MGO+metformin (150 mg/kg/day), and crocin (60 mg/kg/day). MGO was administered orally for 30 days. Starting on day 14, after confirming hyperglycemia, metformin and crocin were administered orally. On day 31, plasma and tissue samples were prepared for experimental assessments. Results: Blood glucose and insulin levels in the MGO group were higher than those in the sham group (p<0.001), and decreased in response to metformin (p<0.001) and crocin treatment (not at all doses). Testis width and volume decreased in the MGO mice and improved in the crocin-treated mice (p<0.05), but not in the metformin group. Superoxide dismutase levels decreased in diabetic mice (p<0.05) and malondialdehyde levels increased (p<0.001). Crocin and metformin improved malondialdehyde and superoxide dismutase. Testosterone (p<0.001) and sperm count (p<0.05) decreased in the diabetic mice, and treatment with metformin and crocin recovered these variables. Luteinizing hormone levels increased in diabetic mice (p<0.001) and crocin treatment (but not metformin) attenuated this increase. Seminiferous diameter and height decreased in the diabetic mice and increased in the treatment groups. Vacuoles and ruptures were seen in diabetic testicular tissue, and crocin improved testicular morphology (p<0.01). Conclusion: MGO increased oxidative stress, reduced sex hormones, and induced histological problems in male reproductive organs. Crocin and metformin improved the reproductive damage caused by MGO-induced diabetes.

• 한국자기공명학회논문지
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• 제22권4호
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• pp.91-100
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• 2018

Benzisothiazolinone (BIT) is the preservative that is widely used in industrial and household products. In this study, zebrafish (Danio rerio) was exposed to BIT at different concentrations (control, 0.5 g/L, 1.0 g/L and 2.0 g/L) for 72 hours. The techniques of nuclear magnetic resonance (NMR) spectroscopy were applied to analyze the effects of BIT on zebrafish. The advantages of NMR are the minimal sample preparation and high reproducibility of experimental results. With the multivariate statistical analysis, dimethylamine, N-acetylaspartate, glycine and histidine were identified as an important metabolite in differentiating between the control and BIT-exposed group. This study will improve the understanding the metabolite changes in the zebrafish in response to BIT exposure.

• 정보화정책
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• 제28권1호
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• pp.64-76
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• 2021

본 연구는 개인정보처리자 관점에서 개인정보보호 활동에 영향을 미치는 요인을 확인하고, 개인정보처리자 스스로 보호 활동을 강화하기 위한 방안을 모색하는데 있다. 요인 탐색을 위해 보호동기이론을 바탕으로 위협평가와 대처평가요인으로 대표되는 주요 요인을 선정하였으며, 요인별 영향분석을 위해 다항로짓모형을 활용하였다. 분석결과, 소규모 개인정보를 보유한 영세 개인정보처리자는 스스로 개인정보 보호 활동을 수행할 수 있도록 보호조치 점검도구 등 시스템 및 기술지원과 인식제고를 위한 교육지원이 필요하다. 그리고 대규모 개인정보를 보유한 개인정보처리자는 예산 및 조세지원 등 개인정보 보호 강화를 위한 투자를 장려하며, 실무 중심의 전문교육 지원이 필요한 것으로 나타났다.

• 한국축산식품학회지
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• 제42권4호
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• pp.609-624
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• 2022

Tenebrio molitor larvae, as known as edible insects, has advantages of being rich in protein, and has been recognized as a suitable alternate protein source for broiler and pig feed. Moreover, given their ability to biodegrade polystyrene, a major pollutant, Tenebrio molitor larvae has been proposed as an innovative solution to environmental problems. In the present study, we investigated the toxicity of Tenebrio molitor larvae powder (TMlp) ingested with expanded-polystyrene (W/ eps) through in vitro and in vivo experiments. The objective of this study was to determine whether TMlp W/ eps can be applied as livestock alternative protein source. For in vitro experiments, cytotoxicity test was performed to investigate the effects of TMlp-extract on the viability of estrogen-dependent MCF-7 cells. The possibility of estrogen response was investigated in two groups: Expanded-polystyrene-fed (W/ eps) TMlp group and without expanded-polystyrene-fed (W/o eps) TMlp group. For in vivo experiments, The male Sprague-Dawley rats were divided based on the dosage of TMlp administered and oral administration was performed to every day for 5 weeks. A toxicological assessments were performed, which included clinical signs, food consumption, body and organ weights, hematology, serum chemistry, and hematoxylin and eosin staining of liver and kidney. There were no specific adverse effect of TMlp W/ eps-related findings under the experimental conditions of this study, but further studies on both sexes and animal species differences should be investigated. In conclusion, TMlp W/ eps was considered non-toxic and observed to be applicable as an alternative protein source for livestock feed.

• Journal of Microbiology and Biotechnology
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• 제32권11호
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• pp.1396-1405
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• 2022

Cholera remains a major global public health problem, for which oral cholera vaccines (OCVs) being a valuable strategy. Patients, who have recovered from cholera, develop antibody responses against LPS, cholera toxin (CT), toxin-coregulated pilus (TCP) major subunit A (TcpA) and other antigens; thus, these responses are potentially important contributors to immunity against Vibrio cholerae infection. However, assessments of the efficacy of current OCVs, especially inactivated OCVs, have focused primarily on O-antigen-specific antibody responses, suggesting that more sophisticated strategies are required for inactivated OCVs to induce immune responses against TCP, CT, and other antigens. Previously, we have shown that the toxT-139F allele enables V. cholerae strains to produce CT and TCP under simple laboratory culture conditions. Thus, we hypothesized that V. cholerae strains that express TCP via the toxT-139F allele induce TCP-specific antibody responses. As anticipated, V. cholerae strains that expressed TCP through the toxT-139F allele elicited antibody responses against TCP when the inactivated bacteria were delivered via a mouse model. We have further developed TCP-expressing V. cholerae strains that have been used in inactivated OCVs and shown that they effect an antibody response against TcpA in vivo, suggesting that V. cholerae strains with the toxT-139F allele are excellent candidates for cholera vaccines.

• Nuclear Engineering and Technology
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• 제27권5호
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• pp.701-709
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• 1995

RELAP5 /MOD3/KAERl의 임계유동모델을 위한 실제적인 배출계수들을 9개의 MARVIKEN 임계유동실험 의 평가계산을 통하여 과냉각과 이상임계유동에 대하여 구하였다. 선택된 실험에는 높은 초기 과냉각도와 큰 노즐 세 장비(L/D)인 것들이 포함되었다. 코드의 평가결과는 RELAP5/MOD3/KAERI은 과냉각임계유동을 크게 예측하고 이 상임계유동은 작게 예측함을 보이고 있다. 이러한 결과들을 이용하여 임계유동모델의 실제적인 배출계수들을 반복법으로 정량화 하였다. 실제적인 배출계 수는 과냉각임계유동이 0.89 그리고 이상임계유동이 1.07로 결정되었으며 관련 표준편차는 각 각 0.0349과 0.1189이다. 본 연구로부터 얻어진 결과는 대형냉각재 상실사고의 실제적인 계통반응 계산과 비상노심냉각계통 성능평가에 적용할 수 있다.

• Journal of Periodontal and Implant Science
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• 제52권6호
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• pp.455-465
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• 2022

Purpose: Periodontal diseases are inflammatory conditions that alter the host's response to microbial pathogens. Type 2 diabetes mellitus (T2DM) is a complex disease that affects the incidence and severity of periodontal diseases. This study investigated the gingival crevicular fluid (GCF) levels of colony-stimulating factor-1 (CSF-1) and interleukin-34 (IL-34) in patients with stage III grade C periodontitis (SIII-GC-P) and stage III grade C periodontitis with uncontrolled type 2 diabetes (SIII-GC-PD). Methods: In total, 72 individuals, including 24 periodontally healthy (PH), 24 SIII-GC-P, and 24 SIII-GC-PD patients, were recruited for this study. Periodontitis patients (stage III) had interdental attachment loss (AL) of 5 mm or more, probing depth (PD) of 6 mm or more, radiographic bone loss advancing to the middle or apical part of the root, and tooth loss (<5) due to periodontal disease. Radiographic bone loss in the teeth was also evaluated; grade C periodontitis was defined as a ratio of the percentage of root bone loss to age greater than 1.0. The plaque index (PI), gingival index (GI), presence of bleeding on probing (BOP), PD, and clinical AL were used for clinical periodontal assessments. GCF samples were obtained and analyzed using an enzyme-linked immunosorbent assay. Results: All clinical parameters-PD, AL, GI, BOP, and PI-were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups for both the full mouth and each sampling site (P<0.05). The total IL-34 and CSF-1 levels were significantly higher in the SIII-GC-PD group than in the PH and SIII-GC-P groups (P<0.05), and there were significant differences between the periodontitis groups (P<0.05). Conclusions: These findings suggest that IL-34 and CSF-1 expression increases in patients with SIII-GC-PD. CSF-1 was associated with the inflammatory status of periodontal tissues and T2DM, while IL-34 was associated only with T2DM.