• Journal of Microbiology and Biotechnology
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• v.11 no.5
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• pp.870-875
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• 2001

A DNA fragment, pCKB13, containing two genes encoding Coenzyme a transferase, was isolated from a genomic DNA library of S. marcescens KCTC 2172. The complete nucleotide sequence of the 2,081-bp BamHI fragment on pCKB13 was determined. Sequencing of the fragment led to the identification of two open reading frames showing high homology with two Coenzyme A (CoA) transferases, Acetoacetyl-CoA transferase (Acot) and Succinyl-CoA transferase (Scot), enzymes catalyzing the reversible transfer of CoA from one carboxylic acid to another. The enzyme activity of Coenzyme A transferase increased after introducing the multicopy of the cloned gene in E. coli. The recombinant protein, overexpressed by multicopy and induction with IPTG, was a polypeptide of 42 kDa, as confirmed by SDS-PAGE. The protein was purified to homogeneity through three sequential chromatographic procedures including ion-exchanged DEAE-sepharose, CM-sepharose, and Mono Q.

• The KIPS Transactions:PartD
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• v.13D no.2 s.105
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• pp.183-190
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• 2006

Database workloads may show different resource usages for database applications. Database administrators can enhance the DBMS performances through resource management that reflects workload characteristics. We provide a method that can identify tunable resources from analyzing the relationship between performance indicators and resources. First, we select which performance indicators increase or decrease by expanding resources using a correlation coefficient and a significance level test. Next, we identify resources that can affect the DBMS Performances by using increasing or decreasing performance indicators. We evaluated our method in the TPC-C and TPC-W environments.

• Journal of Species Research
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• v.7 no.3
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• pp.240-247
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• 2018

Though several wood decay fungi have been reported in the world-wide, only about 600 wood decay fungi have been reported in Korea to date. Thus, the objective of this study was to secure resources for the wood decay fungi in Korea. We investigated wood decay fungi in Yeoninsan Provincial Park, Korea, and the collected specimens were identified based on ITS sequence analysis. Two species were unrecorded species in Korea: Postia hirsuta (Polyporales, Basidiomycota) and Hyphodontia reticulata (Hymenochaetales, Basidiomycota). Another species was previously reported without detailed description: Ceriporia alachuana (Polyporales, Basidiomycota). Here, we provided additional detailed microscopic features and phylogenetic analysis of these species.

• International Journal of Computer Science & Network Security
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• v.21 no.3
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• pp.304-310
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• 2021

The paper presents problems of optimization of the synthesis of embedded systems, in particular Pareto optimization. The model of such a system for its design for high-level of abstract is based on the classic approach known from the theory of task scheduling, but it is significantly extended, among others, by the characteristics of tasks and resources as well as additional criteria of optimal system in scope structure and operation. The metaheuristic algorithm operating according to this model introduces a new approach to system synthesis, in which parallelism of task scheduling and resources partition is applied. An algorithm based on a genetic approach with simulated annealing and Boltzmann tournaments, avoids local minima and generates optimized solutions. Such a synthesis is based on the implementation of task scheduling, resources identification and partition, allocation of tasks and resources and ultimately on the optimization of the designed system in accordance with the optimization criteria regarding cost of implementation, execution speed of processes and energy consumption by the system during operation. This paper presents examples and results for multi-criteria optimization, based on calculations for specifying non-dominated solutions and indicating a subset of Pareto solutions in the space of all solutions.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.29-29
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• 2022

The fast-changing climatic conditions make plants to be vulnerable to many abiotic stresses. Drought stress is one of the limiting factors that affect pepper production in water deficient regions. It affects plant growth and development by altering physiological, morphological, and metabolic processes. Breeding drought tolerant varieties is one of the mitigation strategies to overcome the ever increasing drought disaster. Hence, screening of new drought tolerant pepper genotypes is essential. The current study was aimed to identify new drought tolerant genotypes among the collection of pepper genetic resources. In total, 70 pepper genotypes were screened for drought tolerance after exposure to drought stress condition. The pepper genotypes were classified as highly tolerant, intermediate, or severely sensitive to drought stress based on the phenotypic analysis. Consequently, 13 genotypes significantly exhibited higher recovery rate after drought stress and were classified as highly tolerant. Comparative analysis of morphological and physiological parameters and expression of drought responsive genes between tolerant and susceptible pepper genotypes will be presented and discussed. The identified tolerant genotypes will be useful resources for breeding drought tolerant pepper cultivars.

• Journal of the Korea Furniture Society
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• v.10 no.1
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• pp.1-12
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• 1999

Of the vast number of plant taxa in the world, the wood is one of the most useful resources. It is important to identify the fibers of wood and pulp for the plant taxonomy and for the uses, but we do not have enough information on them, on them, especially for the computerizd data. The fiber identification is one of the difficult tasks. In addition to the plant taxonomy and the fiber-using industries, such identification is also important in many other fields, including education. document examiners, etc. For these purpose, the fibers should be exactly distinguished. The TISS system I have programed to identify various woods would also be useful in the identification of fibers by the genus and species in the features of unknown samples and in searching the features of a species based on its scientific name. Such searching programs are being developed in many other countries with a view to searching for the species name by using the features of the cells of the woody materials. With the survey of all the available literature, the features of the fibers of 124 species both of softwood and hardwood were examined under the electron and optical microscopies. Each species were coded and carded by the feature, and the databases were built. The microscopic were inputted into a personal computer program called and by a slide film scanner. The new computer program called TISS 2 was developed using C computer language. Korean language fonts were added to the TISS 2. The TISS 2 can be in adding and searching a image of fiber features both of a known fiber and an unknown fiber. The databases were corded for the DELTA system with was developed by Dallwitz and Paine in Australia, 1986.

• Journal of Microbiology and Biotechnology
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• v.16 no.1
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• pp.68-76
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• 2006

Despites many attempts to explore the microbial diversity in kimchi fermentation, the predominant flora remains controversial to date. In the present study, major lactic acid bacteria (LAB) were investigated in Chinese cabbage kimchi in the early phase of fermention. For the samples over pH 4.0, viable cell counts of Leuconostoc and Pediococcus were $10^6\;cfu/ml$ and below $10^2\;cfu/ml$, respectively, and 20 isolates out of 172 were subjected to a biochemical identification (API 50 CH kit) as well as molecular-typing methods including ITSPCR with a RsaI digestion and 16s rRNA gene sequence analysis for species confirmation. Seven isolates were nicely assigned to Lb. brevis, 6 to Leuconostoc spp. (2 mesenteroides, 2 citreum, I carnosum, I gasicomitatum), 4 to Weissella (3 kimchii/cibaria, 1 hanii) and 2 to other Lactobacillus spp. (1 farciminis, 1 plantarum). On the other hand, the biochemical identification data revealed 9 strains of Lb. brevis, 6 strains of Leuconostocs,2 strains of Lb. plantarum and 1 strain each of Lb. coprophilus and Lactococcus lactis. However, a single isolates, YSM 16, was not matched to the ITS-PCR database constructed in the present study. Two Lb. brevis strains by API 50 CH kit were reassigned to W kimchii/cibaria, Lb. coprophilus or W hanii, respectively, judging from the results by the above molecular typing approaches. As a whole, the identification data obtained by the biochemical test were different from those of ITS-PCR molecular method by about $63\%$ at genus-level and $42\%$ at species-level. The data by the ITS-PCR method conclusively suggest that predominant LAB species is probably heterolactic Lb. brevis, followed by W kimchii/cibaria, Leuc. mesenteroides, and Leuc. citreum, in contrast to the previous reports [3] that Leuc. mesenteroides is the only a predominant species in the early phase kimchi fermentation.

• Journal of Forest and Environmental Science
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• v.31 no.3
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• pp.214-224
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• 2015

The economy of India and so also of many Asian countries depends on bamboos and their uses are not only in domestic items but also in rural housing and raw materials to several industries and germplasm characterization is an important link between the conservation and utilization of plant genetic resources. Classical taxonomic studies of the bamboos are based on floral morphology and growth habit, which can cause problems in identification due to erratic flowering coupled with different biotic agencies and environmental factors. Identification and genetic relationships among accessions of Thamnocalamus falconeri were investigated using morphology and random amplified polymorphic DNAs (RAPD) technique. Analysis started by using 51 vegetative characters and forty two 10-mer primers that allowed us to distinguish different genotypes hailing from different eco- zones of Garhwal Himalayas (India). The selected primers (12) were used for identification and for establishing a profiling system to estimate genetic diversity. A total of 79.33% polymorphism was estimated by using 12 selected primers. The genetic similar analysis was conducted based on binary digits i.e. presence (1) or absence (0) of bands, which revealed a wide range of variability among the species whereas genetic relatedness was quite high based on vegetative characters. Cluster analysis clearly showed two major clusters for both of the markers viz. morphology and RAPD belonging to 10 accessions of T. falconeri. Two major clusters were further divided into minor clusters. Cluster based on RAPD marker showed grouping of accessions of closed locality whereas analogy was reported for vegetative traits. The RAPD technique has the potential for use in species identification and genetic relationships studies of bamboo for breeding program.

• Water for future
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• v.23 no.3
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• pp.319-328
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• 1990

The Parameter Identification of 2-demensional estuarine model was carried out using new output ADI-FDM numerical semi-implicit schem transformed in boundary fitted(BF) - coordinate. The hydrodynamic equations which is coupled with the transport equations were used as basic equations in the model. Thompson's equations were used to transform governing equations into rectangular plane equations and his elliptic grid generation scheme was used to generate curvilinear grid system. in BF - coordinates. The parameters to be identified are friction coefficient and disperse coefficient embedded in the governing equations. The numerical output scheme is tidally averaged salinity model in BF - coordinates. The algorithm to optimize norm of error between observations and calculations is the influence coefficinet algorithm associated with least square criterion. The lumped model is conssidered in identification. This paper was concetrated on checking whether the new output scheme might be useful to identify parameters in estuarine salinity model or not. The proposed method was tested through experimental application with hypothetical simple model. The result of the test shows that the proposed method can be used for parameter identification in estuarine model.

• Food Science and Biotechnology
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• v.16 no.1
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• pp.99-103
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• 2007

The purpose of this study was to develop a simple detection method, possibly at the species-level, that allows for large-scale screening of bifidobacteria. Human fecal samples were plated on MRS-raffinose agar containing cysteine and neomycin sulfate, serving as selective pressure for bifidobacteria, and 0.003%(w/v) bromocresol green. All of the test strains grew well on this medium at $37{\pm}1^{\circ}C$, forming white colonies surrounded by yellow halos, which presented a sharp contrast against the green background. In this disc assay, the required incubation time to develop a yellowish zone varied with the species of Bifidobacterium that was tested, allowing for differential counts and easy identification at the species-level: 10-14 hr for B. bifidum, 20-22 hr for B. catenulatum and B. infantis. and 24-25 hr for B. longum and B. breve. No apparent color was observed for B. angulatum and B. adolescentis 28 hr after inoculation. To evaluate the results of pH indicator-based identification, individual isolates were subjected to a colony-PCR experiment with genus-specific primers. The amplified products from the isolates were in good accordance with those from the reference strains at a level of 95% agreement. These results suggest that the present method could be conveniently applied to cell counts, as well as to the preliminary identification of bifidobacteria from a variety of sample types including human feces, dairy products, and commercial probiotic supplements.