• Title/Summary/Keyword: resource plant

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Biotransformation of Liquiritin in Glycyrrhiza uralensis Fisch Extract into Liquiritigenin by Plant Crude Enzymes (식물 유래 조효소에 의한 감소 Liquiritin의 Liquiritigenin으로의 변환)

  • Park, Min-Ju;Na, In-Su;Min, Jin-Woo;Kim, Se-Yeong;Yang, Deok-Chun
    • Korean Journal of Medicinal Crop Science
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    • v.16 no.2
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    • pp.74-78
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    • 2008
  • Liquiritin in licorice (Glycyrrhiza uralensis Fisch) extract was treated with three different plant crude enzymes (Prunus dulcis enzyme; PDE, P. armeniaca enzyme; PAE and P. persica enzyme; PPE) for biotransformation. The resulting product of liquiritin was analyzed by TLC and HPLC. The ${\beta}glucosidase$ activities of crude enzymes were 259.6 U/g (PDE), 407.6 U/g (PAE) and 445.8 U/g (PPE), respectively. The liquiritin was converted to liquiritigenin after 12 hours of incubation with the crude enzymes. Liquiritigenin content reached its maximum level after the treatment with PPE at $37^{\circ}C$.

The Small GTPase CsRAC1 Is Important for Fungal Development and Pepper Anthracnose in Colletotrichum scovillei

  • Lee, Noh-Hyun;Fu, Teng;Shin, Jong-Hwan;Song, Yong-Won;Jang, Dong-Cheol;Kim, Kyoung Su
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.607-618
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    • 2021
  • The pepper anthracnose fungus, Colletotrichum scovillei, causes severe losses of pepper fruit production in the tropical and temperate zones. RAC1 is a highly conserved small GTP-binding protein in the Rho GT-Pase family. This protein has been demonstrated to play a role in fungal development, and pathogenicity in several plant pathogenic fungi. However, the functional roles of RAC1 are not characterized in C. scovillei causing anthracnose on pepper fruits. Here, we generated a deletion mutant (𝜟Csrac1) via homologous recombination to investigate the functional roles of CsRAC1. The 𝜟Csrac1 showed pleiotropic defects in fungal growth and developments, including vegetative growth, conidiogenesis, conidial germination and appressorium formation, compared to wild-type. Although 𝜟Csrac1 was able to develop appressoria, it failed to differentiate appressorium pegs. However, 𝜟Csrac1 still caused anthracnose disease with significantly reduced rate on wounded pepper fruits. Further analyses revealed that 𝜟Csrac1 was defective in tolerance to oxidative stress and suppression of host-defense genes. Taken together, our results suggest that CsRAC1 plays essential roles in fungal development and pathogenicity in C. scovilleipepper fruit pathosystem.

Screening and Utilization of Antagonistic Plants to Control Northern root-knot Nematode in Ginseng Fields (인삼포장에 발생하는 당근뿌리혹선층의 방제를 위한 길항식물의 탐색)

  • Yang, Kae-Jin;Doh, Eun-Soo;Kim, Kwang-Ho
    • Journal of Ginseng Research
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    • v.20 no.3
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    • pp.331-338
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    • 1996
  • This study was conducted to screen the antagonistic plants on northern root-knot nematode (Meloidogyne) and to utilize those in its control. Egg hatching of M. hapla was found to be inhibited by 17 plant methanol extracts, and 11 plant extracts among them were also toxic to M. hapla second stage juvenile. Egg hatching of iW. hapla was also found to be inhibited by squeezed extracts of Cassia tora and Zea mays, and they were also toxic to M. hapla second stage juvenile. Extracts of Achyranthes japonica, Melia axedrach and Acorus graminens were toxic to M. hapla second stage with a juvenile mortality above 70clc at the 10 folds diluted concent ration and A. graminens was toxic to tested juvenile mortality above 50% at the 100 folds diluted concentration. The toxicity was directly propotional to the diluted concentration of the plant extracts and to the exposure period. Punica granatum, Acorns graminens and Melia axedrach were effective in inhibiting root penetration of JW. hapla juveniles, among of them p. granatum is most effective Percent inhibition of penetration by second and third stage juveniles into tomato slants penetrating by it was 72.7 and 82.4%, respectively.

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A Study on Profitability of Power Plant for Landfill Gas (매립가스 자원화를 위한 가스엔진 발전의 수익성에 관한 연구)

  • Kim, Oh-Woo;Lee, Jeong-Il
    • Korean Business Review
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    • v.19 no.2
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    • pp.69-94
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    • 2006
  • Landfill gas is a mixture of methane and carbon dioxide produced by the bacterial decomposition of organic wastes, and it is considered to produce bad smells and pollute the environment. Economic trials and the developments of landfill gas, as an alternative energy resource, become known at the recent years. Resource development of landfill gas, which is managed by Korea up to now, is for the most part generation using gas engine. Medium BTU and High BTU are considered for the power generation as well. I\10st income of generation using gas engine is selling charge through a power plant. Expecting to manage the power plant for up to 10 years, the analysis based on revenue and expenditure shows when the unit price is 65.2 Won and the operating rate reaches 90%, it is possible to be into the black in 2012 without considering additional financial expense. It was also analyzed that the profit at a unit price of 85 Won under the anticipated rising unit price by the operating rate of 71% is larger than at the operating rate of 90% under limited unit price of 65.2 Won. It means to manage the power plant at a unit price of 65.2 Won and the operating rate must be higher than 90% for economic logicality. If we assume that the operating rate is 90% and it increases the unit price, the unit price must be higher than 85 Won for the management of a power plant. Analysis of changing a unit price, however, might be expected to have a gradual rise of prices. If there is no price rising and additional income related to CDM(Clean Development Mechanism) and emission trading upon Kyoto protocol, the management of a power plant using gas engine will get financial difficulties because of many operating expenses. However, since landfill gas is considered as a worthy energy resource for the guarantee of sustainable development and for the equity between recent generation and future generation, the development of it must be accomplished by the government's additional supporting and efforts under the interest of all stakeholder who are involved.

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New Fungal diseases of Economic Resource Plants in Korea (III) (유용 자원식물의 진균성 신병해(III))

  • 신현동
    • Korean Journal Plant Pathology
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    • v.11 no.3
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    • pp.197-209
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    • 1995
  • This paper is a third report about the new fungal diseases of economic resource plants in Korea. It contains short descriptions on symptoms, occurrence conditions, pathogen, and some phytopathological notes for each of 10 fungal plant diseases. They are angular leaf spot of Achyranthes japonica by Cercospora achyranthis causing leaf spot and defoliation in the shade of plants, leaf spot of Armoracia lapathifolia by Cercospora armoraciae causing leaf spot to blight from the rainy season to autumn, hypophyllous mold of Dioscorea tokoro by Distocercospora pachyderma causing leaf spot and yellowing, hypophyllous mold of Artemisia spp.by Mycovellosiella ferruginea causing leaf spot and yellowing, angular leaf spot of Aralia elata by Pseudocercospora araliae causing velvety leaf spot and defoliation, hypophyllous mold of Lycium chinense by Pseudocercospora chengtuensis causing velvety leaf spot and defoliation from the rainy season to autumn, angular leaf spot of Diospyros lotus by Pseudocercospora disospyri-morrisianae causing leaf spot and defoliation from summer to autumn, brown leaf spot of Impatiens textori by Pseudocercospora nojimae causing leaf spot to blight from the rainy season, leaf spot of Cephalonoplos segetum by Ramularia cirsii causing leaf spot to blight throughout the growing season, and white mold of Leonurus sibiricus by Ramularia leonuri causing leaf spot to blight mostly in autumn.

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Studies on cropping system for year-round forage crops production

  • Kang, Heonil;Lee, Donghyun;Han, Sangcheol;Choi, Insoo;Yun, Eulsoo;Lee, Jongki
    • Proceedings of the Korean Society of Crop Science Conference
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    • 2017.06a
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    • pp.333-333
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    • 2017
  • This study was conducted to establish of cropping system for year-round forage crops production in east-southern part of Korea and investigated their productivity and feed values. Cropping systems were tested in experiment using oat (cv. Highspeed and Darkhorse) in spring and autumn season, corn (cv. Kwangpyeongok) and sorghum (ss-450) in summer season and rye (cv. Gogu) and triticale (cv. Joseong) in winter season. Considering the forage productivity and feed value such as acid detergent fiber (ADF), neutral detergent fiber (NDF) and total digestive nutrients (TDN), this result suggest that three cropping system for year-round forage crops production. The combinations with triticale (winter), corn or sorghum (summer) and oat (autumn) were would be suitable ones. And also the combinations with rye (winter), corn or sorghum (summer) and oat (autumn) were would be suitable. If forage crops cultivation was started in spring season, the combinations with oat (spring), oat (autumn), triticale or rye (winter), corn or sorghum (summer) and oat (autumn) were would be appropriable. For the more suitable cropping system, we are proceeding on verification experiment of year-round forage crops.

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Plant Genetic Resources in Lam Dong province - Vietnam : Brief in medicine plants and wild orchids situation

  • Ket, Nguyen Van;Cho, Joon-Hyeong
    • Korean Journal of Plant Resources
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    • v.22 no.6
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    • pp.571-583
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    • 2009
  • Lam Dong Province of Vietnam has an exceptional diversity of orchids and herbs. At least 920 herb species are widely mentioned in Vietnamese traditional medicinal literature and over 239 Lam Dong wild orchid species are used for attention under the Conservation Vietnamese wild orchids projects. In collaboration with Department of Plant Biotechnology, Dongguk University - South Korea working under supported ODA funds from Korea government (MIFAFF), we will consider how to collecting and preserving these plants in situ or conservating in vitro as a genetic resource.

Isolation and Characterization of Pathogenesis-Related Protein 5 (PgPR5) Gene from Panax ginseng

  • Kim, Yu-Jin;Lee, Jung-Hye;Jung, Dae-Young;Sathiyaraj, Gayathri;Shim, Ju-Sun;In, Jun-Gyo;Yang, Deok-Chun
    • The Plant Pathology Journal
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    • v.25 no.4
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    • pp.400-407
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    • 2009
  • A pathogenesis-related protein (PgPR5) gene that isolated from the leaf of Panax ginseng was characterized. The ORF is 756 bp with a deduced amino acid sequence of 251 residues. The calculated molecular mass of the matured protein is approximately 27.5 kDa with a predicated isoelectric point of 7.80. A GenBank BlastX search revealed that the deduced amino acid of PgPR5 shares highest sequence similarity to PR5 of Actinidia deliciosa (80% identity, 87% similarity). PgPR5 has a C-terminal and N-terminal signal peptide, suggesting that it is a vacuolar secreted protein. The expression of PgPR5 under various environmental stresses was analyzed at different time points using real-time PCR. Our results reveal that PgPR5 is induced by salt stress, chilling stress, heavy metal, UV, and pathogen infection. These results suggest that the PgPR5 could play a role in the molecular defence response of ginseng to abiotic and pathogen attack. This is the first report of the isolation of PR5 gene from the P. ginseng.

Bacterial Community and Diversity from the Watermelon Cultivated Soils through Next Generation Sequencing Approach

  • Adhikari, Mahesh;Kim, Sang Woo;Kim, Hyun Seung;Kim, Ki Young;Park, Hyo Bin;Kim, Ki Jung;Lee, Youn Su
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.521-532
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    • 2021
  • Knowledge and better understanding of functions of the microbial community are pivotal for crop management. This study was conducted to study bacterial structures including Acidovorax species community structures and diversity from the watermelon cultivated soils in different regions of South Korea. In this study, soil samples were collected from watermelon cultivation areas from various places of South Korea and microbiome analysis was performed to analyze bacterial communities including Acidovorax species community. Next generation sequencing (NGS) was performed by extracting genomic DNA from 92 soil samples from 8 different provinces using a fast genomic DNA extraction kit. NGS data analysis results revealed that, total, 39,367 operational taxonomic unit (OTU), were obtained. NGS data results revealed that, most dominant phylum in all the soil samples was Proteobacteria (37.3%). In addition, most abundant genus was Acidobacterium (1.8%) in all the samples. In order to analyze species diversity among the collected soil samples, OTUs, community diversity, and Shannon index were measured. Shannon (9.297) and inverse Simpson (0.996) were found to have the highest diversity scores in the greenhouse soil sample of Gyeonggi-do province (GG4). Results from NGS sequencing suggest that, most of the soil samples consists of similar trend of bacterial community and diversity. Environmental factors play a key role in shaping the bacterial community and diversity. In order to address this statement, further correlation analysis between soil physical and chemical parameters with dominant bacterial community will be carried out to observe their interactions.