• Journal of Plant Biotechnology
• /
• 제47권2호
• /
• pp.164-171
• /
• 2020

Transgenic Alstroemeria plants resistant to Alstroemeria mosaic virus (AlMV) were generated through RNA-mediated resistance. To this end, the friable embryogenic callus (FEC) of Alstroemeria was induced from the leaf axil tissue and transformed with a DNA fragment containing the coat protein gene and 3'-nontranslated region of AlMV through an improved particle bombardment system. The bar gene was used as a selection marker. More than 300 independent transgenic FEC lines were obtained. Among these, 155 lines resistant to phosphinothricin (PPT) were selected under low stringent conditions. After increasing the stringency of PPT selection, 44 transgenic lines remained, and 710 somatic embryos from these lines germinated and developed into shoots. These transgenic shoots were then transferred to the greenhouse and challenged with AlMV. In total, 25 of the 44 lines showed some degree of resistance. PCR analysis confirmed the presence of the viral sequence. Virus resistance was observed at various levels. Establishment of an efficient transformation system for Alstroemeria will allow inserting transgenes into this plant to confer resistance to viral and fungal pathogens. Accordingly, this is the first report on the production of a transgenic virus-resistant Alstroemeria and lays the foundation for alternative management of viral diseases in this plant.

• 한국어병학회지
• /
• 제16권1호
• /
• pp.61-68
• /
• 2003

MICs of 8 chemotherapeutic agents against forty streptococcal isolates were determined. These strains were isolated from diseased olive flounder, Paralichthys olivaceus, and showed 2-5 multiple drug resistance against different antibacterial agents including ampicillin, doxycycline (DOXY), erythromycin, florfenicol, flumequine, norfloxacin, oxolinic acid, and oxytetracycline (OTC). In conjugation experiment, we found transferable R plasmids carrying OTC and DOXY resistance determinant in 3 drug resistance strains analyzed. Six out of 40 isolates showed positive signal in colony hybridization with the R plasmid DNA (pST9) as a probe. It suggests that other types R plasmid different from pST9 is also involving in multiple drug resistance of streptococci isolated from olive flounder.

• Clinical and Experimental Pediatrics
• /
• 제64권1호
• /
• pp.12-20
• /
• 2021

The mean age at menarche (AAM) of Korean females has been rapidly decreasing over the last 50 years; currently, the prevalence of early menarche (<12 years) is 22.3%. Female adolescents who experience early menarche are known to be at greater risk of psychosocial and behavioral problems along with several physical health problems such as menstrual problems. They also tend to achieve a shorter final height and develop obesity. Population-based Korean studies have shown a strong association between early menarche and the risk of obesity, insulin resistance, metabolic syndrome, nonalcoholic fatty liver disease, diabetes, breast cancer, and cardiovascular disease in adulthood. Although the exact mechanism of how early menarche causes cardiometabolic derangement in later adulthood is unknown, childhood obesity and insulin resistance might be major contributors. Recent studies demonstrated that an excessive consumption of fructose might underlie the development of obesity and insulin resistance along with an earlier AAM. A positive association was observed between sugar-sweetened beverages (a major source of fructose) intake and obesity, metabolic syndrome, insulin resistance, and cardiometabolic risk in Korean females. In pediatrics, establishing risk factors is important in preventing disease in later life. In this regard, early menarche is a simple and good marker for the management of cardiometabolic diseases in adulthood. Decreasing one's fructose intake might prevent early menarche as well as the development of obesity, insulin resistance, and cardiometabolic diseases.

• 아태비즈니스연구
• /
• 제12권4호
• /
• pp.313-329
• /
• 2021

Purpose - Digital trade, which started in the early 2000s, is showing a sharp increase due to the recent pandemic. However, despite this proliferation, users' acceptance of innovation is very slow. Cloud services are at the center of digital trade activation. This study aims to contribute to the spread of digital trade through empirical analysis of the resistance factors that hinder the use of cloud service-based digital trade using the innovation resistance theory and the status quo bias theory. Design/Methodology/Approach - In order to achieve the research purpose, this study was conducted with 171 entrepreneurs using cloud service-based digital trade. Structural equation model(SEM) was used through empirical analysis. Findings - As a result of the study, it was found that the complexity of technology, perceived risk, compatibility, and trust in service providers had a significant effect on innovation resistance, and policy trust did not affect innovation resistance. Also, security concerns and institutional trust were analyzed to have a significant effect on the trust of service providers. Research Implications - This study is meaningful to help the rapid diffusion of innovative technologies through empirical analysis of factors that lower the intention to accept cloud service-based digital trade.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2004년도 하계학술대회 논문집 A
• /
• pp.440-442
• /
• 2004

The ground resistance of middle scale ground pole was measured by using the 'fall of potential method' suggested by IEEE However, the measured resistance value was lower than the minimum value required To solve this problem. ground pole was set to be used commonly. In this research. ground resistance was measured using the newly suggested method and the results were analyzed to see if they satisfied the domestic regulation The results of this research will be applied to the management works as well as the establishment of new plan.

• 한국군사과학기술학회지
• /
• 제24권2호
• /
• pp.197-203
• /
• 2021

Mechanical structures of military equipment have been mainly applied with black oxide coating due to the limitation of surface treatment thickness. However, the mechanical structures applied by the black oxide coating treatment is constantly being corroded by calcium chloride and humidity. Since this can cause serious problems in the operation of equipment, a review to improve surface treatment and corrosion resistance is required. Therefore, in this study, surface treatment methods that can enhance corrosion resistance were selected and corrosion resistance performance was verified through experiments describing harsh field conditions. Thus, applying a proven surface treatment method to future military equipment will prevent corrosion.

• 한국안전학회지
• /
• 제38권2호
• /
• pp.15-22
• /
• 2023

In this study, the process and the environment of a melting furnace, located in Workplace A in Chungnam Province was inspected, and the insulation resistances of electrical circuits and motors were measured, and their changes over five years (2017-2021) were analyzed. It was found that the exhaust fan, oil pumps #A and #B, and heater were installed in 2010. Their insulation resistances decreased from 2017. Specifically, the insulation resistance of Oil Pump #A decreased from 100 (㏁) in 2018 to 20(㏁) in 2021, an 80% reduction. The fast decrease in insulation resistance was due to the high temperature of the melting furnace affecting surrounding the electrical facilities. A safety management scheme on the electrical facility of the melting furnace was proposed.

• 대한안전경영과학회:학술대회논문집
• /
• 대한안전경영과학회 2006년도 춘계공동학술대회
• /
• pp.397-406
• /
• 2006

• Journal of Biosystems Engineering
• /
• 제34권6호
• /
• pp.397-403
• /
• 2009

This study was carried out to investigate the present status of subsoil compaction, and subsoil compaction management in orchard as a basic study for developing a self-propelled explosive subsoiler. Subsoil compaction was evaluated using the soil penetration resistance. Soil cone index was measured using the DIK 5520 type cone penetrometer in several fruit farms such as apple, pear, peach and grapes during growing seasons of these fruit in Jecheon, Gamgok, Choongju, Cheonan and Hwasung areas. Most of the subsoil managing machinery were either explosive type or digging type attached to the tractor or power tiller and turning radius of this machine was more than 3-5 m. Many of the farmers wanted to use the subsoiler which can put lime into soil and rupture soil at the same time. For most of the orchard fields, soil penetration resistance in vehicle traffic area was increased quickly and reached about 1.0 MPa in 5 cm soil depth. As the soil depth increased to 15-20 cm, cone penetration resistance reached about 2.0-2.5 MPa which restricted root growth seriously. Thus it was concluded that one of the main reason for increasing the soil compaction in orchard fields is agricultural vehicle traffic. In the vicinity of fruit trees, compaction is not so serious compared to that of the vehicle traffic area, but as the soil depth increased to 20-25 cm, in most of the orchard fields soil penetration resistance reached about 2.0-2.5 MPa which is the root growth-limiting value. Considering the rooting depth of fruit trees which ranged 30-60 cm for apple, pear and peach, and 20-30 cm for grape, it is necessary to loosen the subosoil and improve the subsoil conditions using subsoiler.

• 한국화재소방학회논문지
• /
• 제23권1호
• /
• pp.40-47
• /
• 2009

현재 철골부재의 내화성능 확보를 위해서 다양한 내화피복방법이 적용되고 있으며, 따라서 이중 하나가 내화도료 피복 시스템의 적용이다. 하지만 내화도료 시스템의 경우 시공후 시간경과에 따라 균열, 탈락 등에 의한 내구성 저하 및 이에 따른 내화성능 상실의 우려가 있으며, 이에 따라 건축 설계 및 시공 초기에 시간경과에 따른 내구성 변화를 파악하여 이에 대비할 수 있는 평가방법 및 유지관리 등에 대한고려가 필요하다. 이에 본 연구에서는 시간경과에 따른 내화도료의 내구성 평가 및 이에 대한 관리방안을 제시하기 위하여 영국을 비롯한 기타 국가의 관련 자료를 조사하여 국내에 적용할 수 있는 내화도료 내구성 평가방법 및 유지관리에 필요한 기초 자료를 제시하도록 하였다.