• Title/Summary/Keyword: resistance induction

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Induction of Systemic Resistance against Cucumber mosaic virus in Arabidopsis thaliana by Trichoderma asperellum SKT-1

  • Elsharkawy, Mohsen Mohamed;Shimizu, Masafumi;Takahashi, Hideki;Ozaki, Kouichi;Hyakumachi, Mitsuro
    • The Plant Pathology Journal
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    • v.29 no.2
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    • pp.193-200
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    • 2013
  • Trichoderma asperellum SKT-1 is a microbial pesticide that is very effective against various diseases. Our study was undertaken to evaluate T. asperellum SKT-1 for induction of resistance against yellow strain of Cucumber mosaic virus (CMV-Y) in Arabidopsis plants. Disease severity was rated at 2 weeks post inoculation (WPI). CMV titre in Arabidopsis leaves was determined by indirect enzyme-linked immunosorbent assay (ELISA) at 2 WPI. Our results demonstrated that among all Arabidopsis plants treated with barley grain inoculum (BGI) of SKT-1 NahG and npr1 plants showed no significant reduction in disease severity and CMV titre as compared with control plants. In contrast, disease severity and CMV titre were significantly reduced in all Arabidopsis plants treated with culture filtrate (CF) of SKT-1 as compared with control plants. RT-PCR results showed increased expression levels of SA-inducible genes, but not JA/ET-inducible genes, in leaves of BGI treated plants. Moreover, expression levels of SA- and JA/ET-inducible genes were increased in leaves of CF treated plants. In conclusion, BGI treatment induced systemic resistance against CMV through SA signaling cascade in Arabidopsis plants. While, treatment with CF of SKT-1 mediated the expression of a majority of the various pathogen related genes, which led to the increased defense mechanism against CMV infection.

$H_2$ $O_2$ Resistance of Escherichia coli That Expresses Acetyl Xylan Esterase of Streptomyces coelicolor A3(2) (Streptomyces coelicolor A3(2)의 Acetyl Xylan Esterase를 발현하는 Escherichia coli의 과산화수소 저항성)

  • Kim Jae-heon;Choi Won-ill;Youn Seock-won;Jung Sang Oun;Oh Chung-Hun
    • Korean Journal of Microbiology
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    • v.40 no.3
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    • pp.232-236
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    • 2004
  • We investigated hydrogen peroxide resistance of Escherichia coli possessing acetyl xylan esterase(AxeA) of Streptomyces coelicolor A3(2). The induction of AxeA production by isopropyl-$\beta$-thiogalactoside was confirmed by SDS-polyacrylamide gel electrophoresis. The differences in growth between induced and non-induced E. coli were determined by the changes in optical density of cultures after hydrogen peroxide treatment The lethal effect of hydrogen peroxide was observed for non-induced cultures at all concentrations tested in this study (lmM, 2.5mM and 5mM). However, cultures induced for AxeA production resisted the lethal effect, except at 5mM where cells were killed irrespective of the AxeA production. The axeA induction increased survival against 1.5mM hydrogen peroxide from 59% to 74%. In addition, AxeA producing E. coli showed increased survival at $45^{\circ}C$, near maximum growth temperature. Therefore, it was concluded that AxeA conferred a cross-resistance upon the bacterium against both oxidative- and heat stress.

Fluorescent Pseudomonas Induced Systemic Resistance to Powdery Mildew in Mulberry (Morus spp.)

  • Pratheesh Kumar, Padinjare Mannath;Sivaprasad, Vankadara
    • International Journal of Industrial Entomology and Biomaterials
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    • v.35 no.2
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    • pp.63-70
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    • 2017
  • Native fluorescent pseudomonas bacteria were isolated from rhizosphere soil of mulberry and were evaluated against powdery mildew. In vitro conidial germination study showed significant (P<0.05) variation in conidial germination by bacterial strains Pf1 and Pf3. Mildew incidence was significantly varied due to treatment with various pseudomonas strains in vivo. Significantly (P<0.05) less mildew incidence was in plants treated with the bacterial strain Pf1 (9.11%) followed by Pf3 (13.48%) controlling 69.40% and 54.75% respectively compared with untreated control. Similarly, mildew severity was least (8.51%) in plants treated with strain Pf1 followed by Pf5 (9.23%) and Pf3 (9.72%) controlling the severity by 84.51%, 77.01% and 71.96% respectively compared with control. The bacterial strains significantly influenced biochemical constituents such as chlorophyll, protein and soluble sugar content of the mulberry leaf. Similarly, bacterial strains significantly increased the activity of the peroxidase (PO) and Polyphenol oxydase (PPO) activity from $7^{th}$ day up to the $28^{th}$ day after treatment. The strain Pf1, Pf3 and Pf5 exhibited a marked enhancement in the peroxidase at different periods of infection. Significant (P<0.01) negative correlation was found between powdery mildew severity with phenol content ($R^2=0.67$) as well as peroxidase ($R^2=0.92$) and polyphenol oxidase ($R^2=0.72$) activity thus confirms induction of systemic resistance in mulberry by pseudomonas bacteria. The study shows scope for exploration of rhizosphere fluorescent pseudomonas bacteria for induction of systemic resistance in mulberry to contain powdery mildew disease effectively.

Effects of Induction Heat Bending Process on Microstructure and Corrosion Properties of ASME SA312 Gr.TP304 Stainless Steel Pipes

  • Kim, Nam In;Kim, Young Sik;Kim, Kyung Soo;Chang, Hyun Young;Park, Heung Bae;Sung, Gi Ho
    • Corrosion Science and Technology
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    • v.14 no.3
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    • pp.120-126
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    • 2015
  • The usage of bending products recently have increased since many industries such as automobile, aerospace, shipbuilding, and chemical plants need the application of pipings. Bending process is one of the inevitable steps to fabricate the facilities. Induction heat bending is composed of compressive bending process by local heating and cooling. This work focused on the effect of induction heat bending process on the properties of ASME SA312 Gr. TP304 stainless steel pipes. Tests were performed for base metal and bended area including extrados, intrados, crown up, and down parts. Microstructure was analyzed using an optical microscope and SEM. In order to determine intergranular corrosion resistance, Double Loop Electrochemical Potentiokinetic Reactivation (DL-EPR) test and ASTM A262 practice A and C tests were done. Every specimen revealed non-metallic inclusion free under the criteria of 1.5i of the standard and the induction heat bending process did not affect the non-metallic inclusion in the alloys. Also, all the bended specimens had finer grain size than ASTM grain size number 5 corresponding to the grain sizes of the base metal and thus the grain size of the pipe bended by induction heat bending process is acceptable. Hardness of transition start, bend, and transition end areas of ASME SA312 TP304 stainless steel was a little higher than that of base metal. Intergranular corrosion behavior was determined by ASTM A262 practice A and C and DL-EPR test, and respectively step structure, corrosion rate under 0.3 mm/y, and Degree of Sensitization (DOS) of 0.001~0.075% were obtained. That is, the induction heat bending process didn't affect the intergranular corrosion behavior of ASME SA312 TP304 stainless steel.

Self Tuning PI Controller of Induction Motor using Fuzzy Control (퍼지제어를 이용한 유도전동기의 자기동조 PI제어기)

  • Nam, Su-Myeong;Lee, Jung-Chul;Lee, Hong-Gyun;Lee, Young-Sil;Chung, Dong-Hwa
    • Proceedings of the KIEE Conference
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    • 2004.10a
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    • pp.173-175
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    • 2004
  • This paper presents a novel design of a self tuning PI controller of induction motor using fuzzy control. In this approach, the fuzzy tuning of a PI controller gains is achieved through fuzzy rules deduced from many robustness simulation tests applied to several induction motors, for a variety of operating conditions such as response to speed command from standstill, step load torque application and speed variations, with nominal parameters and an changed rotor resistance, self inductance and inertia. Simulation results on a speed controller of induction motor are presented to show the effectiveness of the proposed gain tuner. And this controller is better than the fixed gains one in terms of robustness, even under great variations of operating conditions and load disturbance.

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Sensorless Control of Induction Motor Drives Using an Improved MRAS Observer

  • Kandoussi, Zineb;Boulghasoul, Zakaria;Elbacha, Abdelhadi;Tajer, Abdelouahed
    • Journal of Electrical Engineering and Technology
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    • v.12 no.4
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    • pp.1456-1470
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    • 2017
  • This paper presents sensorless vector control of induction motor drives with an improved model reference adaptive system observer for rotor speed estimation and parameters identification from measured stator currents, stator voltages and estimated rotor fluxes. The aim of the proposed sensorless control method is to compensate simultaneously stator resistance and rotor time constant variations which are subject of large changes during operation. PI controllers have been used in the model reference adaptive system adaptation mechanism and in the closed loops of speed and currents regulation. The stability of the proposed observer is proved by the Lyapunov's theorem and its feasibility is verified by experimentation. The experimental results are obtained with an 1 kW induction motor using Matlab/Simulink and a dSPACE system with DS1104 controller board showing the effectiveness of the proposed approach in terms of dynamic performance.

The Analysis and Experimental Investigation of the Diagnosis of Rotor Faults for the Squirrel Cage Induction Motor (농형유도전동기의 회전자 불량진단에 관한 해석 및 실험적 고찰)

  • Kim, Chang-Eob;Chung, Gyo-Bum
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.21 no.3
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    • pp.27-34
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    • 2007
  • The rotor faults of induction motors may cause bad effects on the performance of the induction motor. This paper proposes the detecting technique of these faults by analyzing the waveform of the induced current and voltage of search coil using numerical analysis and the experiment. Several defective rotor bars are simulated to analyze the fault conditions-broken bars and high resistance of rotor bars. In order to prove the usefulness of the proposed method, we made an prototype experimental apparatus. The waveform of the induced voltages in search coil has the obvious characteristics and it is easy to differentiate the normal rotor from the abnormal one. The experimental results show that the proposed method is useful to detect the rotor fault conditions.

Design of an Adaptive Backstepping Controller for Doubly-Fed Induction Machine Drives

  • Dehkordi, Behzad Mirzaeian;Payam, Amir Farrokh;Hashemnia, Mohammad Naser;Sul, Seung-Ki
    • Journal of Power Electronics
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    • v.9 no.3
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    • pp.343-353
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    • 2009
  • In this paper, a nonlinear controller is proposed for Doubly-Fed Induction Machine (DFIM) drives. The nonlinear controller is designed based on an adaptive backstepping control technique, using a fifth order model of an induction machine in the synchronous d & q axis rotating reference frame, whose d axis coincides with the space voltage vector of the main AC supply, and using the rotor current and stator flux components as state variables. The nonlinear controller can perfectly track the torque reference signal measured in the stator terminals under the condition of unity power factor regulation, in spite of the stator and rotor resistance variations. In order to make the drive system capable of operating in the motoring and generating modes below and above the synchronous speed, two level Space-Vector PWM (SV-PWM) back-to-back voltage source inverters are employed in the rotor circuit. It is confirmed through computer simulation results that the proposed control approach is effective and valid.

Speed-Sensorless Induction Motor Control System using a Rotor Speed Compensation (회전자 속도보상을 이용한 센서리스 유도전동기 제어 시스템)

  • Jeong Gang-Youl
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.54 no.3
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    • pp.154-161
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    • 2005
  • This paper proposes a speed-sensorless induction motor control system using a rotor speed compensation. To explain the proposed system, this paper describes an induction motor model in the synchronous reference frame for the vector control. The rotor flux is estimated by the rotor flux observer using the reduced-dimensional state estimator technique. The estimated rotor speed is directly obtained from the electrical frequency, the slip frequency, and the rotor speed compensation with the estimated q-axis rotor flux. The error of the rotor time constant is indirectly reflected in the rotor speed compensation using the compensation of the flux error angle. To precisely estimate the rotor flux, the actual value of the stator resistance, whose actual variation is reflected, is derived. An implementation of pulse-width modulation (PWM) pulses using an effective space vector modulation (SVM) is briefly mentioned. For fast calculation and improved performance of the proposed algorithm, all control functions are implemented in software using a digital signal processor (DSP) with its environmental circuits. Also, it is shown through experimental results that the proposed system gives good performance for the speed-sensorless induction motor control.

Fininte Element Analysis of Squirrel-cage Induction Motor Taking into account the End-ring (엔드링을 고려한 농형 유도전동기의 2차원 유한요소해석)

  • Ha, Gyeong-Ho;Hong, Jeong-Pyo;Kim, Gyu-Tak;Im, Tae-Bin
    • The Transactions of the Korean Institute of Electrical Engineers B
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    • v.48 no.2
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    • pp.49-55
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    • 1999
  • This paper proposes an efficient 2D Finite Element Method(FEM) taking into account the end-ring of three phase squirrel-cage induction motors. The parameters of the squirrel-cage induction motor such as conductivity of secondary conductor have an effect on the characteristics of a motor. Especially, if the characteristic analysis is done without considering the end-ring, the good results can not be obtained. Therefore, we calculated a new resistivity of the secondary conductor including the end-ring's resistance to apply the 2D FEM. Then, the performances of the motors are analyzed by using the new resistivity of secondary conductor which contains the end-ring resistivity. The validity of the proposed method is verified by comparing the numerical results with experimental ones.

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