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$H_2$ $O_2$ Resistance of Escherichia coli That Expresses Acetyl Xylan Esterase of Streptomyces coelicolor A3(2)  

Kim Jae-heon (단국대학교 미생물학과)
Choi Won-ill (단국대학교 미생물학과)
Youn Seock-won (단국대학교 의학레이저연구센터)
Jung Sang Oun (단국대학교 의학레이저연구센터, 국립환경연구원 환경위해성연구부 위해성평가과)
Oh Chung-Hun (단국대학교 의학레이저연구센터)
Publication Information
Korean Journal of Microbiology / v.40, no.3, 2004 , pp. 232-236 More about this Journal
Abstract
We investigated hydrogen peroxide resistance of Escherichia coli possessing acetyl xylan esterase(AxeA) of Streptomyces coelicolor A3(2). The induction of AxeA production by isopropyl-$\beta$-thiogalactoside was confirmed by SDS-polyacrylamide gel electrophoresis. The differences in growth between induced and non-induced E. coli were determined by the changes in optical density of cultures after hydrogen peroxide treatment The lethal effect of hydrogen peroxide was observed for non-induced cultures at all concentrations tested in this study (lmM, 2.5mM and 5mM). However, cultures induced for AxeA production resisted the lethal effect, except at 5mM where cells were killed irrespective of the AxeA production. The axeA induction increased survival against 1.5mM hydrogen peroxide from 59% to 74%. In addition, AxeA producing E. coli showed increased survival at $45^{\circ}C$, near maximum growth temperature. Therefore, it was concluded that AxeA conferred a cross-resistance upon the bacterium against both oxidative- and heat stress.
Keywords
acetyl xylan esterase; heat; hydrogen peroxide; stress resistance; Streptomyces coelicolor A3(2);
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