• Title/Summary/Keyword: resistance determinant

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Cloning and Sequencing of Resistance Determinants to Aminoglycoside Antibiotics from Sterptoalloteichus hindustanus ATCC 31219 (Streptoalloteichus hindustanus ATCC 31219로부터 아미노글라이코사이드계 항생제에 내성을 지정하는 유전자의 클로닝 및 염기서열 결정)

  • Kim, Jong-Woo;Han, Jae-Jin;Choi, Young-Nae;Eom, Joon-Ho;Yoon, Sung-Joon;Hyun, Chang-Gu;Suh, Joo-Won
    • Microbiology and Biotechnology Letters
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    • v.23 no.4
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    • pp.384-389
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    • 1995
  • Streptoalloteichus hindustanus ATCC 31219, a nebramycin complex producer, is similar to Streptomyeces tenebrarius in a viewpoint of resistance to a wide range of aminoglycoside antibiotics. S. tenebrarius has resistance mechanisms of 16s rRNA methylation and aminogycoside modification. However, it is not known whether resistance mechanisms of Stall. hindustanus are the same as in S. tenebrarius. Therefore, we have tried to isolate resistance determinants from Stall. hindustanus. Two different types of aminoglycoside resistance determinants were isolated from Stall. hindustanus and expressed in Streptomyces lividans TK24. The apramycin resistance gene (amr) and the tobramycin resistance gene (tmr) isolated from Stall. hindustanus showed broad resistance spectrum against a dozen of aminoglycoside antibiotics. The complete nucleotide sequences of apramycin resistance gene (amr) were determined. The deduced amino acid sequence of the amr gene of Stall hindustanus ATCC 31219 showed extensive sequence homology to the 16s rRNA methylase gene (kamB) of S. tenebrarius.

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A genetic map of the R-factor pKU10 isolated from pseudomonas putida (Pseudomonas putida에서 분리한 R-factor pKU 10의 유전자 지도)

  • 임영복;민경미;이영록
    • Korean Journal of Microbiology
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    • v.26 no.3
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    • pp.167-172
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    • 1988
  • A genetic map of the IncP-1 group plasmid pKU10 has been prepared through the construction of recombinant plasmids containing various fragments of pKU10. Phenotypic analysis of these derivatives has identified the location of genes encoding resistance to ampicillin, tetracyclin, and chloramphenicol. The region involved in conferring resistance to ampicillin was located around two PstI sites that are 1.0Kb apart. The tetracyclin resistance gene was mapped on the region of HindIII E fragment and a part of HindIII D fragment, and the determinant for chloramphenicol resistance gene was localized on HindIII D fragment.

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Characterization of S. aureus Showing MLS Resistance with Foggy D Shape (fMLS) (안개 D형 저지원을 갖는 MLS 내성 황색 포도상 구균의 분포와 내성 기전)

  • Yoon, Eun-Jeong;Kim, Hyun-Jee;Kwon, Ae-Ran;Choi, Sung-Sook;Shim, Mi-Ja;Choi, Eung-Chil
    • YAKHAK HOEJI
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    • v.50 no.3
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    • pp.199-203
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    • 2006
  • A new type of macrolides, lincosamides and streptogramin B antibiotics (MLS) resistance, showing the characteristic phenotype growing within the inhibition zone area around the clindamycin disk, was identified among clinically isolated Staphylococcus aureus. We named the phenotype as MLS resistance with foggy-D shape (fMLS). The average frequency of MLS isolates was 9%. All of fMLS isolates have only erm(A) for the resistance determinant. The growth pattern of the challenged MLS isolate looks intermediate phase between the patterns of inducible MLS resistance and constitutive MLS resistance.

Plasmid Profiles of Pseudomonas syringae pv. syringae Isolated from Kiwifruit Plants in Korea and the Copper Resistance Determinant (우리나라에서 분리된 참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 플라스미드와 Cu 저항성 유전자)

  • Park, So-Yeon;Han, Hyo-Shim;Lee, Young-Sun;Koh, Young-Jin;Shin, Jong-Sup;Jung, Jae-Sung
    • Korean Journal of Microbiology
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    • v.43 no.4
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    • pp.337-340
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    • 2007
  • Pseudomonas syringae Pv. syringae is a causal agent of bacterial blossom blight of kiwifruit in Korea. Eleven strains of the pathogen were isolated from different kiwifruit orchards in Korea and the plasmid profiles were obtained by pulsed-field gel electrophoresis. They could be clustered into six groups according to the number and size of plasmids. The number of plasmids per strain and size of these plasmids ranged from 0 to 4 and from 22 to 160 kb, respectively. Among them, four strains belonging to Group III which harbored two plasmids were resistant to copper sulfate. Southern blot hybridization of the plasmid DNA indicated that the copper resistance determinant was carried on a 48 kb plasmid.

Strength Estimation Model of Resistance Spot Welding in 780MPa Steel Sheet Using Simulation for High Efficiency Car Bodies (시뮬레이션을 이용한 고효율 차체용 780MPa급 강판의 저항 점 용접 강도 예측 모델 개발)

  • Son, Chang-Seok;Park, Young-Whan
    • Journal of Power System Engineering
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    • v.19 no.2
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    • pp.70-77
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    • 2015
  • Nowadays, car manufacturers applied many high strength steels such AHSS or UHSS to car bodies for weight lightening. Therefore, a variety of applied steel sheet to car bodies increased and the needs of simulation to evaluate weldability also increased in order to reduce the cost and time. In this study, resistance spot welding simulations for DP 780 Steel with 1.0 and 1.4 mm thickness were conducted with respect to lobe curve. 2 regression models to estimate tensile shear strength were suggested and they were second order polynomial regression model and optimized second order regression model. The performance of these models was evaluated in terms of the coefficient of determinant and average error rate.

Clinical characteristics in Taiwanese women with polycystic ovary syndrome

  • Hsu, Ming-I
    • Clinical and Experimental Reproductive Medicine
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    • v.42 no.3
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    • pp.86-93
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    • 2015
  • Polycystic ovary syndrome (PCOS) is one of the most common hormonal endocrine disorders in women of reproductive age. It consists of a heterogeneous collection of signs and symptoms that together form a disorder spectrum. The diagnosis of PCOS is principally based on clinical and physical findings. The extent of metabolic abnormalities in women with PCOS varies with phenotype, body weight, age, and ethnicity. For general population, the prevalence of hyperandrogenism and oligomenorrhea decreases with age, while complications such as insulin resistance and other metabolic disturbances increase with age. Obese women with PCOS have a higher risk of developing oligomenorrhea, amenorrhea, hyperandrogenemia, insulin resistance, and lower luteinizing hormone (LH) to follicle stimulation hormone (FSH) ratios than non-obese women with PCOS. The LH to FSH ratio is a valuable diagnostic tool in evaluating Taiwanese women with PCOS, especially in the diagnosis of oligomenorrhea. Overweight/obesity is the major determinant of cardiovascular and metabolic disturbances in women of reproductive age.

Multiple Drug Resistance and Transferable R-plasmid of Streptococci Isolated from Diseased Olive Flounder (연쇄상구균의 약제내성과 전이성 R-plasmid의 특성)

  • Kim, Jong-Hun;Im, Dae-Hwan;Lee, Chang-Hun;Lee, Su-Jeong;Lee, Wol-Ra;Hwang, Mi-Hye;Kim, Eun-Hui
    • Journal of fish pathology
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    • v.16 no.1
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    • pp.61-68
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    • 2003
  • MICs of 8 chemotherapeutic agents against forty streptococcal isolates were determined. These strains were isolated from diseased olive flounder, Paralichthys olivaceus, and showed 2-5 multiple drug resistance against different antibacterial agents including ampicillin, doxycycline (DOXY), erythromycin, florfenicol, flumequine, norfloxacin, oxolinic acid, and oxytetracycline (OTC). In conjugation experiment, we found transferable R plasmids carrying OTC and DOXY resistance determinant in 3 drug resistance strains analyzed. Six out of 40 isolates showed positive signal in colony hybridization with the R plasmid DNA (pST9) as a probe. It suggests that other types R plasmid different from pST9 is also involving in multiple drug resistance of streptococci isolated from olive flounder.