Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Plasmid Profiles of Pseudomonas syringae pv. syringae Isolated from Kiwifruit Plants in Korea and the Copper Resistance Determinant

우리나라에서 분리된 참다래 꽃썩음병 병원세균(Pseudomonas syringae pv. syringae)의 플라스미드와 Cu 저항성 유전자

  • Park, So-Yeon (Department of Biology, Sunchon National University) ;
  • Han, Hyo-Shim (Department of Biology, Sunchon National University) ;
  • Lee, Young-Sun (Department of Biology, Sunchon National University) ;
  • Koh, Young-Jin (Department of Plant Medicine, Sunchon National University) ;
  • Shin, Jong-Sup (Suncheon-si Agricultural technology and Extension Center) ;
  • Jung, Jae-Sung (Department of Biology, Sunchon National University)
  • Published : 2007.12.30
Download PDF
⟨ Previous Next ⟩

Abstract

Pseudomonas syringae Pv. syringae is a causal agent of bacterial blossom blight of kiwifruit in Korea. Eleven strains of the pathogen were isolated from different kiwifruit orchards in Korea and the plasmid profiles were obtained by pulsed-field gel electrophoresis. They could be clustered into six groups according to the number and size of plasmids. The number of plasmids per strain and size of these plasmids ranged from 0 to 4 and from 22 to 160 kb, respectively. Among them, four strains belonging to Group III which harbored two plasmids were resistant to copper sulfate. Southern blot hybridization of the plasmid DNA indicated that the copper resistance determinant was carried on a 48 kb plasmid.

Pseudomonas syringae pv. syringae는 우리나라에서 참다래 꽃썩음병의 원인세균으로 알려져 있다. 본 연구에서는 우리나라의 서로 다른 참다래 과수원에서 분리되어 동정된 11개 균주의 꽃썩음병균이 가지고 있는 플라스미드 양상을 pulsed-field 젤 전기영동으로 조사하였다. 그 결과 전체 균주들은 가지고 있는 플라스미드의 개수와 크기에 따라 6개 그룹으로 나누어 졌다. 플라스미드의 수는 0에서 4개, 크기는 22 kb에서 160 kb로 다양하였다. 이들 중 두 개의 플라스미드를 가지고 있는 그를 III에 속하는 4균주가 Cu에 대한 저항성을 보였다. Southern blot hybridization 결과 Cu 저항성 유전자는 48 kb 크기의 플라스미드에 들어 있었다.

Keywords

References

  1. Alarcon-Chaidez, F.J., A. Penaloza-Vazquez, M. Ullrich, and C.L. Bender. 1999. Characterization of plasmids encoding the phytotoxin coronatine in Pseudomonas syringae. Plasmids 42, 210-220 https://doi.org/10.1006/plas.1999.1424
  2. Ausubel, F.M., R. Brent, R.E. Kingston, D.D. Moore, J.G. Seidman, J.A. Smith, and K. Struhl. 1999. Current protocols in molecular biology. John Wiley & Sons, Inc., New York, N.Y., USA
  3. Balestra, G.M. and L. Varvaro. 1997. Pseudomonas syringae pv. syringae causal agent of disease on floral bud of Actinidia deliciosa (A. Chev) Liang et Ferguson in Italy. J. Phytopathol. 145, 375-378 https://doi.org/10.1111/j.1439-0434.1997.tb00417.x
  4. Bender, C.L. and D.A. Cooksey. 1986. Indigenous plasmids in Pseudomonas syringae pv. tomato: Conjugative transfer and role in copper resistance. J. Bacteriol. 165, 534-541 https://doi.org/10.1128/jb.165.2.534-541.1986
  5. Bender, C.L., D.K. Malvick, K.E. Conway, S. George, and P. Pratt. 1990. Characterization of pXV10A, a copper resistance plasmid in Xanthomonas campestris pv. vesicatoria. Appl. Environ. Microbiol. 56, 170-175
  6. Coplin, D.L. 1989. Plasmids and their role in the evolution of plant pathogenic bacteria. Annu. Rev. Phytopathol. 27, 187-212 https://doi.org/10.1146/annurev.py.27.090189.001155
  7. Huang, T.C. and T.J. Burr. 1999. Characterization of plasmids that encode streptomycin-resistance in bacteria epiphytes of apple. J. Appl. Microbiol. 86, 741-751 https://doi.org/10.1046/j.1365-2672.1999.00719.x
  8. Kobayashi, D.Y., S.J. Tamaki, and N.T. Keen. 1990. Molecular characterization of avirulence gene D from Pseudomonas syringae pv. tomato. Mol. Plant-Microbe Interact. 3, 94-102 https://doi.org/10.1094/MPMI-3-094
  9. Koh, Y.J., H.J. Chung, and J.H. Kim. 1993. Blossom rot of kiwifruit caused by Pseudomonas syringae. Kor. J. Plant Pathol. 9, 300-303
  10. Long, S.R. and B.J. Staskawicz. 1993. Prokaryotic plant parasites. Cell 73, 921-935 https://doi.org/10.1016/0092-8674(93)90271-Q
  11. Mellano, T. and D.A. Cooksey. 1988. Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae. pv. tomato. J. Bacteriol. 170, 2879-2883 https://doi.org/10.1128/jb.170.6.2879-2883.1988
  12. Nakajima, M., M. Goto, K. Akutsu, and T. Hibi. 2004. Nucleotide sequence and organization of copper resistance genes from Pseudomonas syringae pv. actinidiae. Eur. J. Plant pathol. 110, 223-226 https://doi.org/10.1023/B:EJPP.0000015360.88352.a9
  13. Nakajima, M., M. Goto, and T. Hibi. 2002. Similarity between copper resistance genes from Pseudomonas syringae pv. actinidiae and P. syringae pv. tomato. J. Gen. Plant Pathol. 68, 68-74 https://doi.org/10.1007/PL00013056
  14. Shin, J.S., J.K. Park, G.H. Kim, J.Y. Park, H.S. Han, J.S. Jung, J.S. Hur, and Y.J. Koh. 2004. Identification and ecological characteristics of bacterial blossom blight pathogen of kiwifruit. Res. Plant Dis. 10, 290-296 https://doi.org/10.5423/RPD.2004.10.4.290
  15. Sundin, G.W. and C.L. Bender. 1993. Ecological and genetic analysis of copper and streptomycin resistance in Pseudomonas syringae pv. syringae. Appl. Environ. Microbiol. 59, 1018-1024
  16. Sundin, G.W. and C.L. Bender. 1996. Molecular analysis of closely related copper- and streptomycin-resistance plasmids in Pseudomonas syringae pv. syringae. Plasmid 35, 98-107 https://doi.org/10.1006/plas.1996.0012
  17. Wilkie, J.P., D.W. Dye, and D.R.W. Watson. 1973. Further hosts of Pseudomonas viridiflava. New Zealand J. Agri. Res. 16, 315-323 https://doi.org/10.1080/00288233.1973.10421110