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Plasmid Profiles of Pseudomonas syringae pv. syringae Isolated from Kiwifruit Plants in Korea and the Copper Resistance Determinant  

Park, So-Yeon (Department of Biology, Sunchon National University)
Han, Hyo-Shim (Department of Biology, Sunchon National University)
Lee, Young-Sun (Department of Biology, Sunchon National University)
Koh, Young-Jin (Department of Plant Medicine, Sunchon National University)
Shin, Jong-Sup (Suncheon-si Agricultural technology and Extension Center)
Jung, Jae-Sung (Department of Biology, Sunchon National University)
Publication Information
Korean Journal of Microbiology / v.43, no.4, 2007 , pp. 337-340 More about this Journal
Abstract
Pseudomonas syringae Pv. syringae is a causal agent of bacterial blossom blight of kiwifruit in Korea. Eleven strains of the pathogen were isolated from different kiwifruit orchards in Korea and the plasmid profiles were obtained by pulsed-field gel electrophoresis. They could be clustered into six groups according to the number and size of plasmids. The number of plasmids per strain and size of these plasmids ranged from 0 to 4 and from 22 to 160 kb, respectively. Among them, four strains belonging to Group III which harbored two plasmids were resistant to copper sulfate. Southern blot hybridization of the plasmid DNA indicated that the copper resistance determinant was carried on a 48 kb plasmid.
Keywords
copper resistance gene; kiwifruit; plasmid; Pseudomonas syringae Pv. syringae;
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