• Korean Journal of Breeding Science
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• v.43 no.4
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• pp.273-281
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• 2011

Fusarium head blight (FHB), also known as scab, caused mainly by Fusarium graminearum is a devastating disease of wheat in regions that are warm and humid during flowering. In addition to significant yield and quality losses, the mycotoxin deoxynivalenol produced by the pathogen in infected wheat kernels is a serious problem for food and feed safety. Twenty- three Korean cultivars and "Sumai 3", which is a FHB-resistant Chinese cultivar were tested for Type I, Type II resistances of FHB. Three cultivars were identified as resistant in Type I assessment, and two cultivars were resistant in Type II assessment. Genetic variation and relationship among the cultivars were evaluated on the basis of 11 Simple Sequence Repeat (SSR) and 29 Sequence Tagged Site (STS) markers that were linked to FHB resistance Quantitative Trait Loci (QTL) on chromosome 3BS. One SSR and 7 STS markers detected polymorphisms. Especially, using a STS marker (XSTS3B-57), 32.4% of the variation for Type II FHB resistance could be explained. Genetic relationship among Korean wheat cultivars was generally consistent with their released year. These markers on chromosome 3BS have the potential for accelerating the development of Korean wheat cultivars with improved Fusarium head blight resistance through the use of marker-assisted selection.

• Horticultural Science & Technology
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• v.32 no.1
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• pp.129-132
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• 2014

A new resistant cabbage variety 'CT-171' to Fusarium wilt was bred by crossing $A337MSBC_5$ with resistance to the disease and 397-$G_6$ with good density and color. 'CT-171' was selected after combining ability, seed gathering and regional adaptability test in 2008. For breeding of resistant varieties, we investigated the development of Fusarium wilt on cabbage seedlings inoculated with Fusariumoxysporum f. sp. conglutinans by root dipping inoculation method. As a result, 'CT-171' showed higher resistance to Fusarium wilt than 'Asiaball' used as control. The maturity of new variety was 58 days and was faster than control and well suited for autumn cultivation because of cold resistance. The anthocyanin pigment of plant was not revealed. The weight, height and width of head were 1.5 kg, 14 cm and 15 cm, respectively and the core size was 5.7 cm and stable in various cultivation environments. 'CT-171' which showed good agricultural character and resistance to Fusarium wilt filed for variety protect right in Korea Seed & Variety Service on February 2013. The new variety will be appropriate for export and domestic consumption.

• Horticultural Science & Technology
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• v.19 no.1
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• pp.54-59
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• 2001

The objective of this research was to develop a screening system for selection of grape downy mildew (Plasmopara viticola)-resistant grape cultivars or seedlings under greenhouse condition. Inoculum concentrations ranging from $10^4$ to $10^5spores/mL$ for screening of resistance were evaluated and $5{\times}10^4spores/mL$ was optimum. Of the tested 14 grape cultivars, Campbell Early and Niagara were resistant to grape downy mildew, Sheridan and Cheongsoo were moderate resistant, while Kaiji, Red Queen and Ruby Okuyama were susceptible under both greenhouse and field conditions. Vitis vinifera grape cultivars were more susceptible to grape downy mildew than V. vinifera-labrusca hybrids and V. vinifera-labrusca-aestivalis hybrids. In V. vinifera-labrusca hybrids, tetraploid grape cultivars were more susceptible than diploid culivars. The evaluated results of grape downy mildew resistance under greenhouse condition were in accordance with those of field condition. Results of this study indicated that both greenhouse and field procedures could be used to screen grape cultivars and seedlings for downy mildew resistance.

• Journal of Plant Biotechnology
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• v.41 no.2
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• pp.65-72
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• 2014

Plant cells from which the cell walls have been enzymatically or mechanically removed are called protoplasts. The protoplasts are theoretically totipotent and can be used as sources of somatic cell fusion in practical breeding programs. Wild Solanum species have often been used as sources of important agricultural traits including diverse disease resistance. However, they cannot often be directly applied to breeding programs due to their sexual incompatibility with S. tuberosum. Somatic hybridization via protoplast fusion is one of the ideal methods to overcome this limitation and to introgress certain traits into S. tuberosum. This technique has still widely been used in potato since the first fusion was reported in 1970s. Therefore, this review highlights general perspectives of protoplast fusion and discusses the application of protoplast fusion in potato breeding.

• Journal of the Korean Society of Tobacco Science
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• v.9 no.2
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• pp.11-24
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• 1987

Lines were developed from the two crosses, BY4 X NC 82 and BY 4 X BY 104, by bulk, backcross and anther-derived dihaploid methods of breeding , and their variations in major agronomic, chemical characters, heritability, and response to selection were compared. 1. The anther-derived dihaploid lines showed a greater genetic variability and heritability in characters investigated than the lines developed by the other two methods of breeding . However, those were Inferior In agronomic characters and yielded 15.4% less leaf tobacco than the lines developed by the bulk method. 2. The lines developed by bulk method were higher In the frequency of lines resistant to bacterial wilt and in the yield, but Its lines appeared later flowering. inferior leaf Quality, and higher total nitrogen contents than those developed by the backcross method of breeding. 3. In the population of the lines developed by the backcross method, bacterial wilt resistant lines were less frequent, but the lines with a good leaf quality were more frequent.

• The Plant Pathology Journal
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• v.37 no.2
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• pp.115-123
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• 2021

The purpose of this study was to determine the virulence structure of oat powdery mildew (Blumeria graminis f. sp. avenae, Bga) populations in Poland collected in 2014 and 2015. Powdery mildew isolates were collected from 18 locations in Poland. In total, nine lines and cultivars of oat, with different mildew resistance genes, were used to assess virulence of 180 isolates. The results showed that a significant proportion of the Bga isolates found in Poland were virulent to differentials with Pm1, Pm3, Pm6, and Pm3 + Pm8 genes. In contrast Pm4, Pm5, Pm2, and Pm7 genes were classified as resistant to all pathogen isolates used in the experiment. Based on obtained results we can state that there are differences in virulence pattern and diversity parameters between sites and years, but clear trends are not deducible.

• Proceedings of the Korean Society of Crop Science Conference
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• 2022.10a
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• pp.202-202
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• 2022

Recently days, soybean production in paddy field is increasing, from 4,422 ha in 2016 to 10,658 ha in 2021 in Korea. It is easy for Phytophthora stem and root rot (PSR) occurring in paddy field condition, when it is poorly drained soils with a high clay content, and temporary flooding and ponding. Therefore PSR resistant soybean cultivar is required. The objective of this study is to identify QTL region and candidate genes relating to PSR resistance of the race in main soybean cultivation area in Korea. 210 soybean materials including cultivars and germplasm were used for inoculation and genome-wide association study (GWAS). Inoculation was conducted using stem-scar method with 2 replications in 2-year for the race 3053 from Kimje and 3617 from Andong. 210 materials were genotyped with Soya SNP 180K chip, and structure analysis and association mapping were conducted with QTLMAX V2. The results of inoculation showed that survival ratio ranged from 0% to 96.7% and mean 9.7% for 3053 and ranged from 0% to 100% and mean 7.6% for 3617. Structure analysis showed linkage disequillibrium (LD) was decayed below r²=0.5 at 335kb of SNP distance. Significant SNPs (LOD>7.0) were identified in Chr 1, 2, 3, 4, 5, 11, 14, 15 for 3053 and Chr 1, 2, 3, 7, 10, 14 for 3617. Especially, LD blocks (AX-90455181;15,056,628bp~AX-90475572;15,298,872bp) in Chr 2 for 3053 and 3067 were duplicated. 29 genes were identified on these genetic regions including Glyma.02gl47000 relating to ribosome recycling factor and defense response to fungus in Soybase.

• The Plant Pathology Journal
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• v.32 no.6
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• pp.519-527
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• 2016

Purple blotch, caused by Alternaria porri (Ellis) Cifferi, is a serious disease incurring heavy yield losses in the bulb and seed crop of onion and garlic worldwide. There is an immediate need for identification of effective resistance sources for use in host resistance breeding. A total of 43 Allium genotypes were screened for purple blotch resistance under field conditions. Allium cepa accession 'CBT-Ac77' and cultivar 'Arka Kalyan' were observed to be highly resistant. In vitro inoculation of a selected set of genotypes with A. porri, revealed that 7 days after inoculation was suitable to observe the disease severity. In vitro screening of 43 genotypes for resistance to A. porri revealed two resistant lines. An additional 14 genotypes showed consistent moderate resistance in the field as well as in vitro evaluations. Among the related Allium species, A. schoenoprasum and A. roylei showed the least disease index and can be used for interspecific hybridization with cultivated onion. Differential reaction analysis of three A. porri isolates (Apo-Chiplima, Apn-Nasik, Apg-Guntur) in 43 genotypes revealed significant variation among the evaluated Allium species (P = 0.001). All together, the present study suggest that, the newly identified resistance sources can be used as potential donors for ongoing purple blotch resistance breeding program in India.

• The Plant Pathology Journal
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• v.35 no.4
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• pp.372-380
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• 2019

Pseudomonas syringae pv. actinidiae (Psa) is by far the most important pathogen of kiwifruit. Sustainable expansion of the kiwifruit industry requires the use of Psa-tolerant or resistant genotypes for the breeding of tolerant cultivars. However, the resistance of most existing kiwifruit cultivars and wild genotypes is poorly understood, and suitable evaluation methods of Psa resistance in Actinidia have not been established. A unique in vitro method to evaluate Psa resistance has been developed with 18 selected Actinidia genotypes. The assay involved debarking and measuring the lesions of cane pieces inoculated with the bacterium in combination with the observation of symptoms such as callus formation, sprouting of buds, and the extent to which Psa invaded xylem. Relative Psa resistance or tolerance was divided into four categories. The division results were consistent with field observations. This is the first report of an in vitro assay capable of large-scale screening of Psa-resistance in Actinidia germplasm with high accuracy and reproducibility. The assay would considerably facilitate the breeding of Psa-resistant cultivars and provide a valuable reference and inspiration for the resistance evaluation of other plants to different pathogens.

• Journal of Plant Biotechnology
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• v.31 no.1
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• pp.7-11
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• 2004

This study was carried out to establish a new breeding scheme which is connected with conventional breeding method and anther culture method. To develop a herbicide resistant and direct seeding rice, $F_1$ plants were subjected to anther culture and regenerated plants from 5 crosses were studied to confirm the introduction of bar gene. After PCR analysis, we selected 227 plants which were carrying herbicide resistance gene (bar) out of 1,508 regenerated plants from anther culture. Among 169 $A_2$ lines carrying herbicide resistant gene from 5 crosses including YR23235 (Dongjin Ds3(Ba $r^{R}$)/ Milyang165), 42 lines that had superior agronomic characters were selected for further research. Among them, YR23235Acp79 which showed herbicide resistance, direct seeding adaptability and superiority in major agronomic characters was named Milyang 204. This breeding scheme proved that the anther culture of $F_1$ plants crossed between transformant and cultivar or transform ant alone could be utilized in breeding programs for a rapid progeny fixation and development of a variety.y.