• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.31-37
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• 1993

To analyze and classify the varietal variation of alkali digestibility in detail, which is closely connected with the gelatinization temperature and physical characteristics of cooked rice, the patterns of alkali decomposition changed along the alkali concentration were investigated for thirty three Korean leading rice cultivars and new breeding lines(japonica : 25, Tongil-type:8) including five glutinous rice. Principal component analysis was used to condense the information and to classify rice materials according to decomposed reaction pattern at several levels of potassium hydroxide(KOH) concentration. Thirty three rice varieties were classified largely into four groups by the distribution on the plane of upper two principal component scores which contained above 92% of total informations. Group I was consisted of one variety, Dobongbyeo, which owned almost same strong resistance to alkali digestion at the range of 0.8% to 1.6% KOH solutions. Group II included three japonica and Tongil-type glutinous rice varieties, which revealed medium alkali digestion value(ADV) at 1.4% KOH solution and intermediate change in ADV from 0.8% to 1.6% KOH solutions. Most of Tongil-type and early-maturity japonica rice, which exhibited medium-high ADV at 1.4% of KOH concentration and large ADV difference between low and high alkali solutions, were contained in Group III. Group N included most of medium or medium-late-maturity japonica, which showed high ADV at 1.4% KOH and medium or intermediate-high ADV change between low and high alkali solutions. The 1st principal component indicated the average index of ADV through 0.8-1.6% KOH solutions and the 2nd principal component pointed out the factor related with ADV difference between low and high alkali solutions or regression coefficients of ADV change along with the KOH concentrations.

• Journal of Plant Biotechnology
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• v.42 no.4
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• pp.290-297
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• 2015

Based on the place of its origin, pear tree (Pyrus spp.) is largely divided into European pears (P. communis, cultivated mainly in Europe and the U.S.) and Asian pears (P. pyrifolia, P. bretschneideri, and P. ussuriensis, distributed and grown in East Asian countries including China, Japan, and Korea). Most pear trees have 17 chromosomes (diploidy, 2n=2x=34). Their genetic studies and precise cultivar breeding are highly restricted by conditions such as self-incompatibility controlled by S-locus and juvenility as one major character of fruit crops. Genetic studies on Pyrus have been promoted by the development of various molecular markers. These markers are being utilized actively in various genetic studies, including genetic relationship analysis, genetic mapping, and QTL analysis. In addition, research on pear genetic linkage maps has been extended to studies for the identification of QTL for target traits such as disease resistance and genetic loci of useful traits. NGS technology has radically reduced sequencing expenses based on massive parallel reactions to enable high-capacity and high-efficiency. NGS based genome analyses have been completed for Chinese pear 'Danshansuli' and European pear 'Bartlett'. In Korea, GWAS for agricultural valuable traits such as floral structure, ripening, and total soluble contents have been conducted through resequencing. GBS has been performed for 'Whangkeumbae', 'Cheongsilri', and 'Minibae'.

• Korean Journal of Medicinal Crop Science
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• v.15 no.5
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• pp.339-345
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• 2007

Field performance and morphological characterization was conducted on seven transgenic lines of Codonopsis lanceolata expressing ${\gamma}-TMT$ gene. The shoots were obtained from leaf explants after co-cultivation with Agrobacterium tume-faciens strain LBA 4404 harboring a binary vector pYBI 121 that carried genes encoding ${\gamma}-Tocopherol$ methyltransferase gene (${\gamma}-TMT$) and a neomycin phosphotransferase II gene (npt II) for kanamycin resistance. The transgenic plants were transferred to a green house for acclimation. Integration of T-DNA into the $T_0\;and\;T_1$ generation of transgenic Codonopsis lanceolata genome was confirmed by the polymerase chain reaction and southern blot analysis. The progenies of transgenic plants showed phenotypic differences within the different lines and with relative to control plants. When grown in field, the transgenic plants in general exhibited increased fertility, significant improvement in the shoot weight, root weight, shoot height and rachis length with relation to the control plants. However, all seven independently derived transgenic lines produced normal flower with respect to its shape, size, color and seeds number at its maturity. Indicating that the addition of a selectable marker gene in the plant genome does not effect on seed germination and agronomic performance of transgenic Codonopsis lanceolata. $T_1$ progenies of these plants were obtained and evaluated together with control plant in a field experiment. Overall, the agronomic performance of $T_1$ progenies of transgenic Codonopsis lanceolata showed superior to that of the seed derived non-transgenic plant. In this study, we report on the morphological variation and agronomic performance of transgenic Codonopsis lanceolata developed by Agrobacterium transformation.

• Journal of Plant Biotechnology
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• v.34 no.1
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• pp.11-17
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• 2007

Previously developed transgenic watermelon rootstocks (gongdae) inserted by CGMMV-CP were examined to test the virus tolerance levels. In the restricted plastic house, the $T_{3}$ watermelon rootstock showed tolerance to CGMMV until 70 days after inoculation on the leaves while the non-transformed watermelon rootstock became susceptible at 20 days after inoculation. In the field, tolerance efficiency of transgenic rootstocks maintained up to 40% at 71 days after contamination with CGMMV in the soil while all of the non-transformed rootstocks became susceptible at 37 days with the same condition. In the same field, transgenic rootstocks showed more tolerance to CGMMV than the non-transformed rootstocks as those were inoculated on the leaves, but it showed only 10 days delay before being susceptible. Therefore, transgenic rootstocks have a characteristic of delay effect against CGMMV susceptibility, rather than resistance character. From $T_{3}$ rootstocks homozygous for the CGMMV-CP horticulturally favorable individuals were selected for further breeding and a transgenic line was finally obtained at the $BC_{1}T_{5}$. A material transfer experiment was conducted to find out if the DNA, RNA or expressed protein in the transgenic rootstocks could move to the grafted scion (non-transformed watermelon, Super-Kumcheon). PCR, northern, and western blot analysis were performed and no evidence of transferring of those materials from rootstock to scion was ever found.

• Korean Journal of Agricultural Science
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• v.33 no.2
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• pp.123-127
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• 2006

This study was carried out to compare growth and yield performance for soil conditions at the Maize Genetics and Breeding Lab. in Chungnam National University. The waxy hybrid corn in the drained soil of paddy field showed higher fresh yield per 10a and 100-kernel weight per plant than control. The average dry yield of drained and drainless soil were 804kg/10a and 498kg/10a compared with a control (751kg/10a), respectively. The waxy hybrid corn in the drained soil also had better good stand and stay green characteristics than drainless soil condition, and showed moderately resistance to water stress and weed. Vegetational feature of weeds was characterized as the prevalence of Acalypha auatralis, Echinochloa crus-galli, Persicaria hydrpopiper in drained paddy soil and Persicaria hydrpopiper, Acalypha auatralis, Echinochloa crus-galli in drainless paddy soil, and Echinochloa crus-galli, Acalypha auatralis in upland soil.

• Korean Journal of Plant Resources
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• v.29 no.5
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• pp.625-629
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• 2016

A new Gomchwi cultivar ‘Gommany’ was bred by crossing between Gomchwi (Ligularia fischeri (Ledeb.) Turcz.) and Handaeri-gomchwi (Ligularia fischeri var. spiciformis Nakai). The selection and investigation of growth and yield characteristics were conducted from 2006 to 2011 in field and greenhouse of Highland Agriculture Research Institute, NICS, Rural Development Administration. The color of petiole ear was purple. Petiole trichome existed, but light of leaf back did not. Density of leaf vein was 5 degree in a newly developed cultivar ‘Gommany’. Plant height, leaf length, leaf width and petiole length were 47.7, 16.3, 21.2, and 31.4 ㎝, respectively in the 3 year of growth characteristics. Plant size was also higher than that of ‘Gondalbi’. Bolting and flowering time were Aug. 5 and Sept. 10, respectively. In contrast, Bolting time of ‘Gondalbi’ showed 25 days quite earlier bolting time compared to ‘Gommany’, Which led to earlier flowering time. ‘Gommany’ showed higher number of leaves (138) per plant compared to ‘Gondalbi’ (130). Furthermore, yield was 97% higher in ‘Gommany’ (1,575 g/plant) than in ‘Gondalbi’ (798 g/plant). ‘Gommany’ showed higher leaf thickness (0.69㎜) than ‘Gonalbi’ (0.46 ㎜), and consequently showed more hardness in leaf characteristics (24.3 ㎏/㎠) compared to ‘Gondalbi’ (23.0㎏/㎠). ‘Gommany’ showed higher resistance in the susceptibility of powdery mildew compared to ‘Gondalbi’. ‘Gommany’ cultivar was registered as a novel variety with number 90 in The Minister of the Korea Forest Service on December 2015.

• Journal of Plant Biotechnology
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• v.30 no.1
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• pp.19-25
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• 2003

Radiation technique in agriculture was initiated to develop mutant rice. Seeds of Daechungbyeo rice were irradiated with 250 Gy gamma ray for the purpose of inducing and selecting rice variants. Some quantitative traits of the variants in M$_{8}$ generation were evaluated and RAPD analysis was carried out. Variants showed a wider range of agronomic characteristics in both a positive and a negative direction compared with their original variety. The new mutants were characterized by an increased or decreased in plant height, lodging resistance and shorter panicle. RAPD analysis showed that polymorphic bands were presented in most of the primers. In comparison with the original variety, variants were classified into four groups through UPGMA analysis. Among mutants no. 91, 139, 140 and 141 was ranked as salt tolerance and the proline content of these mutants was more increased than that of original variety. The lines of 139, 140 and 141 had the highest genetic distance as compared to original variety in the dendrogram. It is expected that such variants will be useful not only for studying molecular genetics but also for breeding research and genetic analysis.s.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.5
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• pp.649-654
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• 1995

This study was conducted to investigate the utility and agronomic characteristics and use of cytoplasmic. male-sterile (CMS) haploid plants derived from interspecific cross between (male-sterile NC82$\times$burley21) F$_1$ plant (Nicotiana tabacum L.) and Nicotiana africana. Abundant seeds of high germinability were obtained when Nicotiana tabacum (cytoplasmic male-sterile F$_1$ plants) is pollinated by Nicotiana africana. Most of seedlings died at the cotyledonary stage. The remaining seedlings are viable F$_1$ hybrids or maternal haploids that can be easily distinguished. Number of interspecific Fl hybrids and matermal haploids per capsule obtained from the interspecific cross between cytoplasmic male-sterile tobacco F$_1$ plants and N.africana yielded 2.2 and 0.5 plants, respectively. Out of 149 CMS haploid plants obtained from interspecific cross, 102 plants showed green type while the others were yellow type for leaf and stem. This results agreed with the genetic ratios expected among haploid plants from the F$_1$ hybrids heterozygous for two recessive genes of yellow color of burley tobacco plant. Out of 83 CMS haploid plants inoculated with TMV, 48 plants showed resistant, while the others was susceptible. It agreed with the expected genetic ratios for a single dominant gene of TMV resistance. CMS haploid plant will be useful as a source material for breeding of CMS doubled haploid lines

• Korean Journal of Plant Resources
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• v.27 no.4
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• pp.371-379
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• 2014

In order to determine the response of turnip leaves to various races of Botrytis cinerea, fourteen different races were infected to the leaf surface and measured the lesion size. Based on lesion size of the pathogen on the leaves, turnip showed susceptible response to four races, moderate to seven races and resistant to three races. Four glucosinolate (GLS) compounds, such as butenyl GLS, indole-3ylmethyl GLS (I3M), 4-methoxy-indole-3-ylmethyl GLS (4MOI3M) and normal methoxy-indole-3ylmethyl GLS (NMOI3M) were identified in turnip leaves infected with Botrytis cinerea. Leaves infected with resistant races showed higher GLS contents as compared with the leaves infected with susceptible races. Contents of I3M in the leaves with resistant races were 2.5 times as high as those in uninfected leaves, whereas I3M in the leaves with susceptible races showed lower contents than those in untreated leaves. Leaves infected with resistant races showed 4MOI3M and NMOI3M contents 2.3 and 2.7 times as high as those in uninfected leaves, respectively. GLSs in the infected leaves were most abundant at 5~10 mm area from center of the lesion. However, the GLSs in 5~10 mm area were rapidly degradated at leaves with susceptible races which resulted in continuous expansion of the lesion on the leaves, whereas no degradation was obseveved at leaves with resistant races. These results suggest that accumulation and degradation of GLS compounds in turnip leaves closely related to the susceptibility and resistance of turnip leaves to Botrytis races.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.648-651
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• 2013

A new cultivar 'Daewang' strawberry was developed by the National Institute of Horticultural & Herbal Science for forcing culture in 2010. The cultivar 'Daewang' was originated from the cross between 'Maehyang', a high firmness cultivar and 'Wongyo 3111', a high sugar content in 2006. The cultivar shows erect plant type, vigorous growth habit, early flower bud differentiation with 12-15 flowers per cluster from planting on healthy nursery. Fruits of 'Daewang' are conical type having a bright red skin color, and 16-17 g in an average weight. 'Daewang' is suitable for forcing culture as time required for dormancy breaking ranged between 50 and 100 hours. This cultivar has excellent taste for high sugar/acid ratio as sugar content of $11.1^{\circ}Brix$, acidity of 0.39% with abundant texture and can be harvested by late spring because the fruit firmness of 'Daewang' was $18.2g{\cdot}mm^{-2}$ that was about $7.9g{\cdot}mm^{-2}$ higher than $10.3g{\cdot}mm^{-2}$ of 'Akihime' cultivar. But although total yield is not significantly different from 'Akihime' cultivar, its marketable yield is remarkably higher than that of 'Akihime' cultivar. Disease and pest resistance of 'Daewang' have a tendency to sensitive powdery mildew, anthracnose and spotted spider.