• Title/Summary/Keyword: resistance

Search Result 31,214, Processing Time 0.049 seconds

Functional Metagenome Mining of Soil for a Novel Gentamicin Resistance Gene

  • Im, Hyunjoo;Kim, Kyung Mo;Lee, Sang-Heon;Ryu, Choong-Min
    • Journal of Microbiology and Biotechnology
    • /
    • v.26 no.3
    • /
    • pp.521-529
    • /
    • 2016
  • Extensive use of antibiotics over recent decades has led to bacterial resistance against antibiotics, including gentamicin, one of the most effective aminoglycosides. The emergence of resistance is problematic for hospitals, since gentamicin is an important broad-spectrum antibiotic for the control of bacterial pathogens in the clinic. Previous study to identify gentamicin resistance genes from environmental samples have been conducted using culture-dependent screening methods. To overcome these limitations, we employed a metagenome-based culture-independent protocol to identify gentamicin resistance genes. Through functional screening of metagenome libraries derived from soil samples, a fosmid clone was selected as it conferred strong gentamicin resistance. To identify a specific functioning gene conferring gentamicin resistance from a selected fosmid clone (35-40 kb), a shot-gun library was constructed and four shot-gun clones (2-3 kb) were selected. Further characterization of these clones revealed that they contained sequences similar to that of the RNA ligase, T4 rnlA that is known as a toxin gene. The overexpression of the rnlA-like gene in Escherichia coli increased gentamicin resistance, indicating that this toxin gene modulates this trait. The results of our metagenome library analysis suggest that the rnlA-like gene may represent a new class of gentamicin resistance genes in pathogenic bacteria. In addition, we demonstrate that the soil metagenome can provide an important resource for the identification of antibiotic resistance genes, which are valuable molecular targets in efforts to overcome antibiotic resistance.

The Ground Resistance Reduction Effect of o Conductive Expansion Ground Resistance Reducing Material (도전성 팽창 접지저감재의 접지저항 저감 효과)

  • Kim, Jong-Wook;Kim, Kyung-Chul;Choi, Jong-Kee;Kang, Min-Gyu;Baek, Nam-Woong
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
    • /
    • v.22 no.2
    • /
    • pp.155-161
    • /
    • 2008
  • The grounding systems provide a low resistance path for fault or normal currents into the earth. The material for reducing ground resistance is buried around the grounding electrode to increase its effective size of the electrode and thus to obtain low ground resistance. Increasing the contacting area with the grounding electrode is effective for lowering the ground resistance in soils. Using this idea the conductive expansion ground resistance reducing material was developed. In this paper experimental data has shown that the ground resistance reduction method using proposed material is superior to using a typically available commercial ground resistance reducing material.

Effects of Resistance and Resistance & Stabilization Exercises on the Strength of Lumbar and Lower Limbs of the Elderly

  • Choi, Seok Joo;Kim, Jong Woo;Hwang, Byeong Jun
    • Journal of International Academy of Physical Therapy Research
    • /
    • v.4 no.2
    • /
    • pp.611-617
    • /
    • 2013
  • The purpose of this study is to identify the effective lumbar area exercise program to prevent falls of the elderly by dividing 14 female elders over 65 living in Daegu into two groups such as resistance exercise group and resistance & stabilization exercise group, and applying a 60 minute exercise program three times a week for 12 weeks. The followings are the results. First, both of resistance exercise and resistance & stabilization exercise brought improvement of lumbar strength and there was no significant difference between two groups. Second, there was no significant difference in lower limb strength in resistance exercise group but resistance & stabilization exercise group showed significant increase in lower limb flexor strength. To make a summary of the above results, it can be said that 12 weeks' resistance & stabilization exercise is effective to the lumbar strength and lower limb strength of the elderly. Accordingly, if combining resistance exercise and stabilization exercise appropriately, we can build a successful preventive program which even the elderly who cannot perform resistance exercise easily can follow.

말뚝기초의 연적 방향 극한하중

  • 김명모
    • Proceedings of the Korean Geotechical Society Conference
    • /
    • 2002.11a
    • /
    • pp.209-236
    • /
    • 2002
  • Ultimate pile capacity - Point resistance - Frictional resistance - Determination of point and frictional resistances from field tests - Summary of recommendations from design Group effects Settlement analysis.

  • PDF

How Can Marketers Overcome Consumer Resistance to Innovations? - The Investigation of Psychological and Social Origins of Consumer Resistance to Innovations - (마케팅관리자들이 어떻게 혁신에 대한 소비자저항을 극복할 수 있는가? - 혁신에 대한 소비자의 개인적 사회적 저항의 근원 탐색 -)

  • Bagozzi, Richard P.;Lee, Kyu-Hyun
    • Journal of Global Scholars of Marketing Science
    • /
    • v.15 no.3
    • /
    • pp.211-231
    • /
    • 2005
  • It is important for marketers to understand both individual resistance and group resistance in order to successfully bring innovations into global markets. We suggest that consumers resist innovations as individuals and as members of a group and that they do this in different ways at different stages of decision-making. The individual resistance begins with forms of initial resistance, develops into emergent resistance and mature or belated resistance at the individual level. In addition, personal moral standards can influence decision making in relation to the adoption of innovations. Individual resistance is sometimes accompanied by or evolves into group resistance. We introduce a framework for thinking about consumer resistance to innovations that sees it as a consequence of social identity, which has functions for the individual, the group to which one belongs, and other individuals and groups. Consumers with membership in a certain group try to increase their self-esteem through the process of social comparison. The more consumers strongly identify with and bond with a certain group, the more in-group solidarity and out-group hostility will occur. Out-group hostility gives group members strong resistance toward products and services related to the out-group. Individual resistance and group resistance are threats to marketers and dampen performance. By considering the existence of resistance to innovations and seeking strategies to overcome it, marketers can transform these threat into new opportunities. A better understanding of consumer resistance can complement research on the adoption of innovations and help in the development of a universal model of consumer behavior.

  • PDF

Behavior of the Temperature Coefficient of Resistance at Parallelly Connected Resistors (병렬로 접속된 저항체에서 저항온도계수의 거동)

  • Lee, Sunwoo
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
    • /
    • v.31 no.2
    • /
    • pp.98-101
    • /
    • 2018
  • In this paper, we discuss the fabrication of metal alloy resistors. We connected them in parallel to estimate their resistance and temperature coefficient of resistance (TCR). The fabricated resistors have different resistances, 5 and $10{\Omega}$ and different TCRs, 50 and $200ppm/^{\circ}C$. Each resistor was confirmed to have the correct atomic composition through the use of energy dispersive X-ray (EDX). The resistors' electrical properties were confirmed by measuring resistance and TCR. The resistance and TCR of the resistors connected in parallel were estimated through the increase in resistance due to the increase in temperature, and were compared with the measured values. We are confident that this TCR estimation technique, which uses the increase in resistance due to temperature, will be very useful in designing and fabricating resistors with low and stable TCR.

Study on Inheritance of Potato virus X Resistance in Capsicum annuum

  • Shi, Jinxia;Choi, Do-Il;Kim, Byung-Dong;Kang, Byoung-Cheorl
    • The Plant Pathology Journal
    • /
    • v.24 no.4
    • /
    • pp.433-438
    • /
    • 2008
  • Potato virus X (PVX) resistance in potato is one of the best-characterized resistance models, however little is known in pepper. To evaluate the resistance to PVX in Capsicum annuum, a total of eleven pepper accessions were used for resistance screening against two PVX strains, USA and UK3. None of them were resistant against strain UK3, whereas four resistant genotypes were found against strain USA, three of which were further characterized. Two unlinked dominant genes were identified for both genotypes Bukang and Perennial; resistance in the genotype CV3 seemed to be conferred by two complementary dominant genes. These results demonstrated that the resistance to PVX in C. annuum is different from that in potato. This is the first report on genetic analysis of PVX resistance in C. annuum.

Estimation of load and resistance factors based on the fourth moment method

  • Lu, Zhao-Hui;Zhao, Yan-Gang;Ang, Alfredo H.S.
    • Structural Engineering and Mechanics
    • /
    • v.36 no.1
    • /
    • pp.19-36
    • /
    • 2010
  • The load and resistance factors are generally obtained using the First Order Reliability Method (FORM), in which the design point should be determined and derivative-based iterations have to be used. In this paper, a simple method for estimating the load and resistance factors using the first four moments of the basic random variables is proposed and a simple formula for the target mean resistance is also proposed to avoid iteration computation. Unlike the currently used method, the load and resistance factors can be determined using the proposed method even when the probability density functions (PDFs) of the basic random variables are not available. Moreover, the proposed method does not need either the iterative computation of derivatives or any design points. Thus, the present method provides a more convenient and effective way to estimate the load and resistance factors in practical engineering. Numerical examples are presented to demonstrate the advantages of the proposed fourth moment method for determining the load and resistance factors.

Effect of the Specific Resistance of Water on Corrosion Characteristics of STS 304 for Gas Boiler (가스보일러용 STS 304의 부식특성에 미치는 용수의 비저항의 영향)

  • Lim, Uh-Joh;Kim, Hwan-Sik;Yun, Byoung-Du
    • Journal of Fisheries and Marine Sciences Education
    • /
    • v.19 no.3
    • /
    • pp.323-328
    • /
    • 2007
  • This paper was studied on the effect of the specific resistance of water on corrosion characteristics of STS 304 for gas boiler. The electrochemical polarization test of STS 304 for gas boiler was carried out. And the polarization resistance, uniform corrosion, corrosion behavior by impressed potential and corrosion sensitivity of STS 304 with specific resistance were considered. The main results are as following: 1) With being low the specific resistance of water, the polarization resistance and corrosion potential of STS 304 decreases, while corrosion current density increases. 2) Under constant impressed potential, the corrosion sensitivity of STS 304 is large with being low the specific resistance due to synergy effect of Cl ion.

A Study on the Mock-up Test of Waterproofing/Root Penetration Resistance Technology for the Application to an Eco-bridge (생태교량 적용을 위한 방수·방근공법의 Mock-up 시험 연구)

  • Seon, Yun-Suk;Choi, Su-Young;Kim, Meong-Ji;Choi, Sung-Min;Oh, Sang-Keun
    • Proceedings of the Korean Institute of Building Construction Conference
    • /
    • 2014.05a
    • /
    • pp.230-231
    • /
    • 2014
  • This study is concerned with the root penetration resistance competence and constructability of a waterproofing and root penetration resistance layer of an eco-bridge which is applied with various types of seeding. For the review of root penetration resistance competence, the competence was tested using herbs, woody plants, and tall trees. As a result, there was no tear of or damage to the waterproofing and root penetration resistance layer as well as the sweeping-down phenomenon of a waterproofing and root penetration resistance layer by soil. Also, the effect of the root growth of herbs, woody plants, and tall trees on the root penetration resistance system was confirmed.

  • PDF