• Biomolecules & Therapeutics
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• v.19 no.1
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• pp.1-8
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• 2011

G-protein coupled receptors (GPCRs) constitute an important class of drug targets and are involved in every aspect of human physiology including sleep regulation, blood pressure, mood, food intake, perception of pain, control of cancer growth, and immune response. Radiometric assays have been the classic method used during the search for potential therapeutics acting at various GPCRs for most GPCR-based drug discovery research programs. An increasing number of diverse small molecules, together with novel GPCR targets identified from genomics efforts, necessitates the use of high-throughput assays with a good sensitivity and specificity. Currently, a wide array of high-throughput tools for research on GPCRs is available and can be used to study receptor-ligand interaction, receptor driven functional response, receptor-receptor interaction,and receptor internalization. Many of the assay technologies are based on luminescence or fluorescence and can be easily applied in cell based models to reduce gaps between in vitro and in vivo studies for drug discovery processes. Especially, cell based models for GPCR can be efficiently employed to deconvolute the integrated information concerning the ligand-receptor-function axis obtained from label-free detection technology. This review covers various platform technologies used for the research of GPCRs, concentrating on the principal, non-radiometric homogeneous assay technologies. As current technology is rapidly advancing, the combination of probe chemistry, optical instruments, and GPCR biology will provide us with many new technologies to apply in the future.

• Journal of muscle and joint health
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• v.21 no.3
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• pp.255-264
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• 2014

Purpose: The purpose of the study were to analyze the research trends of compassion fatigue in Korea nurses and to suggest future research directions. Methods: For this study, RISS, KISTI, KISS, National Assembly Library databases were searched using the key words 'compassion fatigue', 'secondary traumatic stress', 'professional quality of life' that contains compassion fatigue as a sub-element, and 'nurses' for this study. A total of 124 articles published in June 2014 were searched and analyzed 19 articles for the final analysis. Results: The most frequently used study design was descriptive (N=17, 89.5%). High compassion fatigue was reported in this study. Factors affecting compassion fatigue were personal factors (e.g., age, health state, and sleep hours), work-related factors (e.g., experience with traumatized patients), and psychological factors (e.g., job stress, anxiety, excessive empathy, social support, and coping strategy). Finally, Consequence factors of compassion fatigue was reported burnout, silencing response and turnover intention. Conclusion: There was a few studies on compassion fatigue. Further research on compassion fatigue needs to use a variety of study designs instruments suitable for Korean nurses, and to develop management programs on compassion fatigue in nurses.

• Nuclear Engineering and Technology
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• v.46 no.2
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• pp.273-280
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• 2014

The spectrum weighted responses of various detectors were calculated to provide guidance on the proper selection and use of survey instruments on the basis of their energy response characteristics on the neutron fields. To yield the spectrum weighted response, the detector response functions of 17 neutron-measuring devices were numerically folded with each of the produced calibration neutron spectra through the in-house developed software 'K-SWR'. The detectors' response functions were taken from the IAEA Technical Reports Series No. 403 (TRS-403). The reference neutron fields of 21 kinds with 2 spectra groups with different proportions of thermal and fast neutrons have been produced using neutrons from the $^{241}Am$-Be sources held in a graphite pile, a bare $^{241}Am$-Be source, and a DT neutron generator. Fluence-average energy ($E_{ave}$) varied from 3.8 MeV to 16.9 MeV, and the ambient-dose-equivalent rate [$H^*(10)/h$] varied from 0.99 to 16.5 mSv/h.

• Nuclear Engineering and Technology
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• v.40 no.4
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• pp.311-318
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• 2008

In modem power reactors, nuclear fuels have recently reached 55,000 MWd/MtU from the initial average burnup of 35,000 MWd/MtU to reduce the fuel cycle cost and waste volume. At such high burnups, a fuel pellet produces fission products proportional to the burnup and creates a typical high burnup structure around the periphery region of the pellet, producing the so called 'rim effect'. This rim region of a highly burnt fuel is known to be ca. $200\;{\mu}m$ in width and is known to affect the fuel integrity. To characterize the local burnup in the rim region, solid sampling in the micro meter region by laser ablation is needed so that the distribution of isotopes can be determined by ICP-MS. For this procedure, special radiation shielding is required for personnel safety. In this study, we installed a radiation shielded laser ablation ICP-MS system, and a performance test of the developed system was conducted to evaluate the safe operation of instruments.

• Journal of Preventive Medicine and Public Health
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• v.52 no.5
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• pp.281-291
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• 2019

Objectives: The validity of instruments measuring socioeconomic position (SEP) has been a major area of concern in research on cardiovascular health disparities. The purpose of this systematic review is to identify the current status of the methods used to measure SEP in research on cardiovascular health disparities in Korea and to provide directions for future research. Methods: Relevant articles were obtained through electronic database searches with manual searches of reference lists and no restriction on the date of publication. SEP indicators were categorized into compositional, contextual, composite, and life-course measures. Results: Forty-eight studies published from 2003 to 2018 satisfied the review criteria. Studies utilizing compositional measures mainly relied on a limited number of SEP parameters. In addition, these measures hardly addressed the time-varying and subjective features of SEP. Finding valid contextual measures at the organizational, community, and societal levels that are appropriate to Korea's context remains a challenge, and these are rarely modeled simultaneously. Studies have rarely focused on composite and life-course measures. Conclusions: Future studies should develop and utilize valid compositional and contextual measures and appraise social patterns that vary across time, place, and culture using such measures. Studies should also consider multilevel influences, adding a focus on the interactions between different levels of intertwined SEP factors to advance the design of research. More attention should be given to composite and life-course measures.

• Journal of Sensor Science and Technology
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• v.32 no.6
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• pp.412-417
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• 2023

There is increasing interest in the rapid and highly sensitive monitoring of cell viability in biological and toxicological research. Conventional methods depend on optical assays using Water Soluble Tetrazolium-8 (WST-8) or 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) assay, which requires a large volume of samples and special instruments, necessitating shipment of clinical samples to laboratories. This paper reports on the development of a rapid and sensitive electrochemical (EC) sensor using screen printed electrode (SPE) and surface modification using 4'-mercapto-N-phenylquinone diamine (4'-NPQD), as double electron mediators, for monitoring cell viability via the measurement of nicotinamide adenine dinucleotide (NADH). We used the sensor to observe the viability of MCF-7 and doxorubicin (Dox)-treated cells. The oxidation current of NADH was measured via chronoamperometry (CA), and the EC results showed a good linear relationship when compared with NADH quantification using WST-8 assay. The analysis time was only 10 s and limit of detection (LOD) of NADH was 1.78 µM. Our EC method has the potential to replace conventional WST assays for cell viability and cytotoxicity experiments.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.21 no.4
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• pp.543-558
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• 2023

Currently, Japan is undertaking a nationwide project to measure and map radioactive contamination around Fukushima, as part of the efforts to restore normalcy following the nuclear accident. The Japan Atomic Energy Agency (JAEA) manages the Fukushima Environmental Safety Center, located approximately 20 km north of the Fukushima Daiichi nuclear power plant in Minamisōma City, Fukushima Prefecture. In collaboration with the JAEA, this study involved conducting comparison experiments and analyses with radiation detectors in high radiation environments, a challenging task in Korean environments. Environmental radiation surveys were conducted using three types of detectors: CZT, NaI(Tl), and LaBr₃(Ce), across two contaminated areas. Dose rate values were converted using dose rate conversion factors for each detector type, and dose rate maps were subsequently created and compared. The detectors yielded similar results, demonstrating their feasibility and reliability in high radiation environments. The findings of this study are expected to be a crucial reference for enhancing the verification and supplementation of procedures and methods in future radiation measurements and mobile surveys in high-radiation environments, using these three types of radiation instruments.

• Journal of Acupuncture Research
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• v.18 no.4
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• pp.91-100
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• 2001

Background and Objective : Most diagnostic method for numbness were invasive and complex. So we need to simplify and objectify diagnostic method for numbness. Some study with EAV which is one of Physiological Signal Measuring Instruments, report significantly result as objective diagnostic method for other clinical symptom. By using EAV, we have obtained some physiological signal data from meridian-acupoints of 58 numbness cases. Objective and Methods : This study researched into the clinical statistics for 58 case who ware in numbness, and they ware treated with oriental medical care at the Dong-shin university oriental hospital during 1 year from April 3 2000 to March 30 2001. The data were analyzed and interpreted to compare with traditional differentiation of symptom-complexes, then further evaluated as the Five Evolutive Phases to make them differentiated. The EAV valus of Five Evolutive Phases were identified with the sequence of wood(木), fire(火), earth(土), steel(金), water(水). Results and Conclusion : These values of physiological signal were identical with standard differentiation of symptom-complexes of numbness which is the main cause of dishannonious flow of Qi and blood of the in the liver and deficiency of Qi and blood of the bladder with stagnancy of dampness. Among Five Evolutive Phases, Earth and wood values were increased, steel, fire and water were decreased significantly. This data imply the possibility of somewhat generalization from measuring instruments.

• Journal of Acupuncture Research
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• v.26 no.6
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• pp.171-178
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• 2009

Objectives : To investigate the correlation between DITI and assessment instruments of knee OA. Methods : Data were obtained from 127 subjects with OA of the knee. They were asked to answer VAS pain scale, LFI, WOMAC and KHAQ. Correlation was assessed by examing the pearson's correlation and spearman's rank correlation coefficients. Results : The thermal difference between the patella of knee was correlated with VAS, LFI and WOMAC. Age, duration, BMI and sex were not correlated with the thermal differences of each region. Conclusions : DITI value was useful for evaluating the severity of OA. And we should find out the standardization of analysis of DITI value for diagnosis of OA.

• Journal of Korean Society of Transportation
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• v.24 no.1 s.87
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• pp.59-72
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• 2006

In spite of continuous implementation of the transportation demand management (TDM), the profuse use of car at the peak-time has caused chronic traffic congestion in the Seoul downtown area. This study makes a comparative analysis on the effectiveness of commuting cost subsidy system for public transit user with other policy instruments such as an increment in fuel tax and park cost. This study not only follows standard guidelines of stated preference methodology to guarantee objectivity, but also uses sample enumeration method and non-Parametric bootstrapping method to secure reliability of empirical results. As a result of empirical studies, the conversion effect of car to public transit is superior to other two Policy instruments. Also. an increment in fuel tax and park cost is income-regressive from the equity aspect in a wage bracket, but commuting cost subsidy system for Public transit user is Income-progressive As a fundamental research on commuting cost subsidy system for public transit user, this study is likely to Provide Policy-makers with quantitative information useful in establishing Public transport Policy to Promote the use of the public transit.