• Title/Summary/Keyword: research growth

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Selection of an Ethanol Tolerant Clostridium thermohydrosulfuricum Strain

  • PARK, YOUNG-MIN;CHUL-HO KIM;SANG-KI RHEE
    • Journal of Microbiology and Biotechnology
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    • v.2 no.3
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    • pp.226-229
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    • 1992
  • An ethanol tolerant mutant was selected by successive transfers of Clostridium thermohydrosulfuricum ATCC 33223 into the media with progressively higher ethanol concentrations. The growth kinetics of the mutant were characterized under various growth conditions. Physiological differences such as enhanced growth, tolerance to various solvents, alteration of the optimum temperature and the ratio of end products during fermentation were noticed in the mutant.

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Effects of Soil Moisture on the Growth of American Ginseng (Panax quinquefolium L.)

  • Li, Thomas S.C.;Berard, R.G.
    • Journal of Ginseng Research
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    • v.22 no.2
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    • pp.122-125
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    • 1998
  • Adequate available soil moisture level is considered to be one the most important components in growing high yields of good quality ginseng. Excessive soil moisture may promote stillborn fungal pathogens and cause serious diseases in ginseng fields. This study showed that soil moisture levels for optimum growth and health of ginseng varied with soil texture. Fifty- percent available moisture for sandy loam and 75% for silty loam are the best moisture levels for good growth and higher yield.

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Changes in Growth Characteristics, Biological Activity and Active Compound Contents in Ginseng of Different Ages (재배년수에 따른 인삼의 생육특성, 생리활성, 성분의 변화)

  • Moon, Ji Won;Jang, In Bae;Yu, Jin;Jang, In Bok;Seo, Su Jeoung;Lee, Sung Woo
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.6
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    • pp.383-389
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    • 2019
  • Background: Ginseng has been used as a medicine and functional food since ancient times. It is a perennial crop, and its whose commercial valuse increases with growing period and is affected by the atmosphere and soil environment. Methods and Results: In a selected field, we measured air temperature under a shade structure and soil physicochemical properties, and determied plant and root growth as well as ginsenoside and total polyphenol content of one- to five-year-old ginsengs plants. Although air temperature above 30℃ was recored for more than 37 days, no marked growth inhibition of ginseng was detected. Among all soil physicochemical properties, except for pH, were within the allowable range the shortage increases with ginseng years. In five-year-old ginseng, the quantity is about 9.7% higher than the average weight by standard, indicating that is not affected by temperature when grown under a shade structure. Three-year-old ginseng contained the highest total ginsenoside and total polyphenol levels and exhibited the greatest DPPH radical scavenging activity. Conclusions: The total ginsenoside and protopanaxadiol/protopanaxatriol ratio were both low at five-year-old ginseng plants, which was attributed to rapid growth of the root system in five-year-oid plants. There were no significant differences in total polyphenol content and antioxidant activity between.

Growth and Sex Ratio of Juvenile Olive Flounder (Paralichthys olivaceus) at Different Size-ranks

  • Kim, Jong-Hyun;Kim, Hyun-Chul;Noh, Jae-Koo;Lee, Jeong-Ho;Kang, Jung-Ha;Kim, Kyung-Kil
    • Fisheries and Aquatic Sciences
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    • v.10 no.4
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    • pp.191-195
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    • 2007
  • The aim of this study was to assess whether size-rank at the juvenile stage is associated with growth performance and sex ratio in olive flounder (Paralichthys olivaceus). Juveniles (mean body weight, $8.8{\pm}0.9\;g$) were selected from two age groups, with the high size-rank group (HSRG) fish in the upper 19.3-67.3% of 104 days post-hatching (dph) age group and the low size-rank group (LSRG) fish in the lower 9.0-55.7% of 116 dph age group. Triplicate groups of 100 fish were reared over 32 weeks. A heavier final body weight was observed in the HSRG ($360{\pm}12\;g$) compared to the LSRG ($315{\pm}9\;g$, P<0.05), whereas no differences in feed intake, feeding efficiency, and mortality were seen between the experimental groups. The percentage of females was significantly higher in the HSRG (51.7%) than in the LSRG (30.8%, P<0.05). In addition, a remarkable increase in growth was recorded in females, indicating that the relative frequency of females increased with a body weight increase. The results suggest that high size-rank juvenile olive flounder have better growth performance and a higher percentage of females, because females start growing faster than males before size grading occurs.

Induction on in vitro Plant Regeneration the Apple Rootstocks of Fire Blight Resistance by Plant Growth Regulators (생장조절제 처리에 따른 과수화상벙 저항성 사과대목의 기내 식물체 유도)

  • Young Hee Kwon;Won IL Choi;Hee Kyu Kim;Kyung Ok Kim;Ju Hyoung Kim;Yong Sup Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.23-23
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    • 2021
  • Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.

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1-Aminocyclopropane-1-Carboxylate Deaminase from Pseudomonas stutzeri A1501 Facilitates the Growth of Rice in the Presence of Salt or Heavy Metals

  • Han, Yunlei;Wang, Rui;Yang, Zhirong;Zhan, Yuhua;Ma, Yao;Ping, Shuzhen;Zhang, Liwen;Lin, Min;Yan, Yongliang
    • Journal of Microbiology and Biotechnology
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    • v.25 no.7
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    • pp.1119-1128
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    • 2015
  • 1-Aminocyclopropane-1-carboxylate (ACC) deaminase, which is encoded by some bacteria, can reduce the amount of ethylene, a root elongation inhibitor, and stimulate the growth of plants under various environmental stresses. The presence of ACC deaminase activity and the regulation of ACC in several rhizospheric bacteria have been reported. The nitrogen-fixing Pseudomonas stutzeri A1501 is capable of endophytic association with rice plants and promotes the growth of rice. However, the functional identification of ACC deaminase has not been performed. In this study, the proposed effect of ACC deaminase in P. stutzeri A1501 was investigated. Genome mining showed that P. stutzeri A1501 carries a single gene encoding ACC deaminase, designated acdS. The acdS mutant was devoid of ACC deaminase activity and was less resistant to NaCl and NiCl2 compared with the wild-type. Furthermore, inactivation of acdS greatly impaired its nitrogenase activity under salt stress conditions. It was also observed that mutation of the acdS gene led to loss of the ability to promote the growth of rice under salt or heavy metal stress. Taken together, this study illustrates the essential role of ACC deaminase, not only in enhancing the salt or heavy metal tolerance of bacteria but also in improving the growth of plants, and provides a theoretical basis for studying the interaction between plant growth-promoting rhizobacteria and plants.

Methanolic Extract Isolated from Root of Lycoris aurea Inhibits Cancer Cell Growth and Endothelial Cell Tube Formation In Vitro

  • Kang, Moo-Rim;Lee, Chang-Woo;Yun, Ji-Eun;Oh, Soo-Jin;Park, Song-Kyu;Lee, Ki-Ho;Kim, Hwan-Mook;Han, Sang-Bae;Kim, Hyoung-Chin;Kang, Jong-Soon
    • Toxicological Research
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    • v.28 no.1
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    • pp.33-38
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    • 2012
  • In this study, we investigated the effect of methanolic extract isolated from the root of Lycoris aurea (LA) on the growth of cancer cells and the tube formation activity of endothelial cells. Various cancer cells were treated with LA at doses of 0.3, 1, 3, 10 or 30 ${\mu}g/ml$ and LA significantly suppressed the growth of several cancer cell lines, including ACHN, HCT-15, K-562, MCF-7, PC-3 and SK-OV-3, in a dose-dependent manner. We also found that LA induced cell cycle arrest at G2/M phase in ACHN renal cell adenocarcinoma cells. Further study demonstrated that LA concentration-dependently inhibited the tube formation, which is a widely used in vitro model of reorganization stage of angiogenesis, in human umbilical vein endothelial cells. Collectively, these results show that LA inhibits the growth of cancer cells and tube formation of endothelial cells and the growth-inhibitory effect of LA might be mediated, at least in part, by blocking cell cycle progression.

Cultural characteristics and formation of fruiting body in Lentinula edodes (표고버섯의 배양적 특성 및 자실체 형성에 관한 연구)

  • Lee, Won-Ho;Kim, In-Yeop;Ko, Han-Gyu;Kim, Seon-Cheol;Choi, Sun-Gyu;Noh, Jong-Hyun;Park, Heung-Soo
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.24-28
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    • 2014
  • Lentinula edodes is known by oak mushroom. It has been favored as delicious and nutritious food and the low-calorie food with a high nutritional value. It is also functional food since it contains a material well-known for its medicinal benefits. Since the growth and quality of oak mushrooms are sensitively affected by environmental conditions, an adequate environmental control is very essential to improve the yield and quality under protected cultivation. The main objectives of the study were to investigate cultural characteristics of mycelial growth and in vitro fruiting of Lentinula edodes. The optimum culture media for mycelial growth of L. edodes were PDA and MYA. Similarly, optimum temperature was $25^{\circ}C$. Malt extract(2%) and yeast extract(0.2%) were optimum carbon and nitrogen sources. Optimal culture period was 110~120 days in sawdust medium. Mycelial growth in medium(61 mm/7 days) Quercus mongolica extract the most good. Among different five log types, highest mycelial growth and fruiting productivity were observed in Quercus variabilis sawdust(20.9%).

Foliar application of humic acid or a mixture of catechol and vanillic acid enhanced growth and productivity of alfalfa

  • Khaleda, Laila;Kim, Min Gab;Jeon, Jong-Rok;Cha, Joon-Yung;Kim, Woe-Yeon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.37 no.3
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    • pp.248-253
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    • 2017
  • Humic acid (HA) is known to consist of various kinds of polymeric organics, their detailed structures can vary depend on sample sources such as organic manure, composts, peat, and lignite brown coal, and largely exists in grassland soils. HA possesses diverse positive effects that not only increase plant growth but also improve soil fertility. Recently, we have manufactured a co-polymeric product of catechol and vanillic acid (CAVA) synthesized artificially, and found that CAVA as a HA mimic increases seed germination and salt tolerance in Arabidopsis. In this study, we examined whether HA or CAVA affects to seedling growth in alfalfa. Foliar application of HA or CAVA increased alfalfa seedling growth including aerial and in root parts. HA or CAVA dramatically enhanced size of leaf and root, whereas HA significantly displayed higher bioactivity than CAVA. Taken together, CAVA acts like as a HA mimic in alfalfa that could apply as an alternation supplement to enhance plant growth and productivity.