통합 검색 | Korea Science

Lee, Jang Wook;Kim, Jung Eun;Goo, In Bon;Hwang, Ju-Ae;Im, Jea Hyun;Choi, Hye-Sung;Lee, Jeong-Ho
- 한국발생생물학회지:발생과생식
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- 제19권4호
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- pp.181-187
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- 2015
Early life stage mortality in fish is one of the problems faced by loach aquaculture. However, our understanding of immune system in early life stage fish is still incomplete, and the information available is restricted to a few fish species. In the present work, we investigated the expression of immune-related transcripts in loach during early development. In fishes, recombination-activating gene 1 (RAG-1) and sacsin (SACS) have been considered as immunological function. In this study, the expression of the both genes was assessed throughout the early developmental stages of loach using real-time PCR method. maRAG-1 mRNA was first detected in 0 dph, observed the increased mostly until 40 dph. Significant expression of maRAG-1 was detected in 0 to 40 dph. These patterns of expression may suggest that the loach start to develop its function after hatching. On the other hand, maSACS was detected in unfertilized oocyte to molura stages and 0 to 40 dph. maSACS mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred.
https://doi.org/10.12717/DR.2015.19.4.181 인용 PDF KSCI

Hidayat, Asep;Turjaman, Maman;Faulina, Sarah Asih;Ridwan, Fadel;Aryanto, Aryanto;Najmulah, Najmulah;Irawadi, Tun Tedja;Iswanto, Apri Heri
- Journal of the Korean Wood Science and Technology
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- 제47권4호
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- pp.459-471
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- 2019
Several species of Aquilaria and Gyrinops are native to Indonesia and well known as agarwood-producing trees with a high economic value. Their bioactive compounds have a wide spectrum of uses, such as in medicine and cosmetics. These genera have undergone extensive search for novel bioactive compounds. The purpose of this study was to isolate, identify, and characterize the endophytic fungi community associated with Aquilaria malaccensis, A. microcarpa, Gyrinops versteegii, and A. crassna trees and investigate their bioactive properties as antioxidant agents and antagonists. A total of 50 fungi were successfully isolated from different tissues of the four species of agarwood-producing trees. Two isolates exhibited strong antioxidant activity, namely, Apodus oryzae (R2MC3A, $IC_{50}$ 60.92 mg/mL) and Diaporthe sp. (P1DS1[C], $IC_{50}$ 76.65 mg/mL). Two isolates, Pestalotiopsis theae (P3BS3[B]) and Curvularia sp. (P2CD3A), showed >75% antifungal activity against pathogenic Fusarium solani. The results revealed that endophytic fungi associated with the studied agarwood-producing trees had potential antioxidant and antifungal activities for further applications in biotechnology.
https://doi.org/10.5658/WOOD.2019.47.4.459 인용 PDF KSCI HTML