Journal of the Korean Wood Science and Technology

The Korean Society of Wood Science & Technology (한국목재공학회)
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Antioxidant and Antifungal Activity of Endophytic Fungi Associated with Agarwood Trees

  • Hidayat, Asep (Forest Microbiology Laboratory, Forest research and Development Centre, Research, Development and Innovation Agency, Ministry of Environmental and Forestry) ;
  • Turjaman, Maman (Forest Microbiology Laboratory, Forest research and Development Centre, Research, Development and Innovation Agency, Ministry of Environmental and Forestry) ;
  • Faulina, Sarah Asih (Forest Microbiology Laboratory, Forest research and Development Centre, Research, Development and Innovation Agency, Ministry of Environmental and Forestry) ;
  • Ridwan, Fadel (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University) ;
  • Aryanto, Aryanto (Forest Microbiology Laboratory, Forest research and Development Centre, Research, Development and Innovation Agency, Ministry of Environmental and Forestry) ;
  • Najmulah, Najmulah (Forest Microbiology Laboratory, Forest research and Development Centre, Research, Development and Innovation Agency, Ministry of Environmental and Forestry) ;
  • Irawadi, Tun Tedja (Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University) ;
  • Iswanto, Apri Heri (Department of Forest Product, Faculty of Forestry, Universitas Sumatera Utara)
  • Received : 2019.03.16
  • Accepted : 2019.07.15
  • Published : 2019.07.25
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Abstract

Several species of Aquilaria and Gyrinops are native to Indonesia and well known as agarwood-producing trees with a high economic value. Their bioactive compounds have a wide spectrum of uses, such as in medicine and cosmetics. These genera have undergone extensive search for novel bioactive compounds. The purpose of this study was to isolate, identify, and characterize the endophytic fungi community associated with Aquilaria malaccensis, A. microcarpa, Gyrinops versteegii, and A. crassna trees and investigate their bioactive properties as antioxidant agents and antagonists. A total of 50 fungi were successfully isolated from different tissues of the four species of agarwood-producing trees. Two isolates exhibited strong antioxidant activity, namely, Apodus oryzae (R2MC3A, $IC_{50}$ 60.92 mg/mL) and Diaporthe sp. (P1DS1[C], $IC_{50}$ 76.65 mg/mL). Two isolates, Pestalotiopsis theae (P3BS3[B]) and Curvularia sp. (P2CD3A), showed >75% antifungal activity against pathogenic Fusarium solani. The results revealed that endophytic fungi associated with the studied agarwood-producing trees had potential antioxidant and antifungal activities for further applications in biotechnology.

Keywords

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Fig. 1. Dual culture assay between potential endophytic against the pathogen of F. solani after 6 days incubation. A. P. theae P3BS3[B], B. Curvularia sp. (P2CD3A).

Table 1. Antioxidant and antifungal activity of thirteen potential endophytic fungi

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Table 2. Biomass, yield and phytochemical analyisis of crude extract etyl acetate soluble of 13 isolates endophytic fungi after fermentation at 14 days

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Table 3. Identification of 13 potential endophytic fungi based on internal transcribed spacer (ITS) using BLAST analysis

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