• Title/Summary/Keyword: repeat element

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Formation of a New Solo-LTR of the Human Endogenous Retrovirus H Family in Human Chromosome 21

  • Huh, Jae-Won;Kim, Dae-Soo;Ha, Hong-Seok;Kim, Tae-Hong;Kim, Wook;Kim, Heui-Soo
    • Molecules and Cells
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    • v.22 no.3
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    • pp.360-363
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    • 2006
  • Human endogenous retroviruses (HERVs) contribute to various kinds of genomic instability via rearrangement and retrotransposition events. In the present study the formation of a new human-specific solo-LTR belonging to the HERV-H family (AP001667; chromosome 21q21) was detected by a comparative analysis of human chromosome 21 and chimpanzee chromosome 22. The solo-LTR was formed as a result of an equal homologous recombination excision event. Several evolutionary processes have occurred at this locus during primate evolution, indicating that mammalian-wide interspersed repeat (MIR) and full-length HERV-H elements integrated into hominoid genomes after the divergence of Old World monkeys and hominoids, and that the solo-LTR element was created by recombination excision of the HERV-H only in the human genome.

A Study on the melting Characteristics of Fuse Element by Repeating Overcurrent (반복과전류에 의한 퓨즈 엘리먼트의 용단특성에 관한 연구)

  • Kim, Youn-Hyun
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.24 no.2
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    • pp.120-126
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    • 2010
  • This paper propose analysis and examination of the melting characteristic of fuse elements by repeating overcurrent as a depletion factor of high pressure current limiting fuse through test following existence and nonexistence of extinction material and various configuration of elements. To examine deterioration progress rate by repeating overcurrent we analyzed heat for various element notching configuration, designed plate type, ring type element and estimated the relationship with life span by analyzing breaking characteristic through repeating overcurrent test with adjusting load factor at Silicon Dioxide(SiO2) filled state or in air. A Crack by repeat stress, decrease of section and transformation by friction with extinction material by repeating overcurrent causes a problem which shortens life span based on fuse repeating frequency. Since the contents of this paper might be useful to research the correlation between friction of materials and repeating life span based on load factor of repeating current, the quality of product would be improved through solution of the problem.

A Study on the Selection of Optimum Auto-design Data using FEA (유한요소해석을 이용한 최적자동설계 데이터 선정에 관한 연구)

  • 박진형;이승수;김민주;김순경;전언찬
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2001.04a
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    • pp.406-409
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    • 2001
  • This study is an investigation for the ADS optimum design by using FEA. We write out program which express ADS perfectly and reduce the required time for correcting of model to the minimum in solution and manufacture result. We complete algorithm which can plan optimum forming of model by feedback error information in CAE. Then we correct model by feedback date obtaining in solution process, repeat course following stress solution again and do modeling rachet wheel for optimum forming. That is our aim. In rachet wheel, greatest equivalence stress originates in key groove corner and KS standard is proved the design for security.

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A Study on the Possibility of Model Development from Traditional Han-ok to Urban Clustering Housing Model in Korean Context (전통한옥의 도시집합주거로 발전 가능성 연구)

  • Shon, Seung-Kwang
    • Journal of the Korean housing association
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    • v.19 no.3
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    • pp.71-81
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    • 2008
  • A traditional Korean housing is a typical type which is contained life style, spatial organization and scape element of people who lived in Korea. In the hanok, people want to be live not only in human environment and traditional culture, but also modem urban housing as a high density. This article deals as follows: First, Hanok as urban housing would be composed in a housing lot, linear type layout, devide building, and cluster type. Second, Housing unit and configuration of Multi family housing can be used single story, second story, second story + single story, multi story and Hanok roofing. Thirds, structure of the building are traditional wooden, combined one of steel and wood or concrete and wood, and the building system in exterior and interior can be seperated into another system. Forth, Image of multi story Hanok A last, consistency of Hanok is not a repeat of an origin but application and creative aptitude of the origin, and multi family housing application of Hanok can be a trial creative.

A study on Improvement of Workpiece Deformation In High Frequency Heat Treatment (고주파 열처리에서 공작물 변형 개선에 관한 연구)

  • Hong, Sung-Oh;Kim, Hong-Bae;Cho, Gyu-Jae
    • Journal of the Korean Society of Manufacturing Process Engineers
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    • v.2 no.2
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    • pp.31-36
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    • 2003
  • Base and saddle feed drive system in machine tool puts in operation for Improvement of life and endure by high frequency heat treatment. In this time, work requirement of establishment to gets by repeat experimentation. In this paper, using the finite elementary method, we predict and revision processing, and gets minimizing of deformation and reduce the progress of Grinding works. Moreover, having high frequency heat treatment, the maximum deformation genesis m the middle parts without slideway length. Take deformation Into finite element program (ANSYS) of taper process in roughing process, after having high frequency heat treatment, existed quantity of deformation can be reduced down to 80 percents.

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Development of X-Y table with LPM and its user's interface (LPM을 이용한 X-Y table과 사용자 interface의 개발)

  • Kim, Moon-Hwan;Kim, Young-Joo;Ahn, Jong-Bo;Lee, Sang-Jeong
    • Proceedings of the KIEE Conference
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    • 1995.07a
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    • pp.291-293
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    • 1995
  • LPM(Linear pulse motor) was developed as a prototype. In this paper, the design results of prototype and its dynamic characteristics are shown. The prototype was designed by using permeance analysis and the thrust value was also confirmed by comparing with the analysis results of the finite element method. To verify the performance of LPM. dynamic characteristics were measured. In slewing characteristic of the LPM, the thrust value of prototype LPM is higher than commercial level. A X-Y table was structured as a application of the prototype LPM. A controller and user's interface was also developed. By using this control system, the position error of X-Y table was checked. The repeat position error in linear movement was under than ${\pm}$ 100 micron.

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Gene-editing techniques and their applications in livestock and beyond

  • Tae Sub Park
    • Animal Bioscience
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    • v.36 no.2_spc
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    • pp.333-338
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    • 2023
  • Genetic modification enables modification of target genes or genome structure in livestock and experimental animals. These technologies have not only advanced bioscience but also improved agricultural productivity. To introduce a foreign transgene, the piggyBac transposon element/transposase system could be used for production of transgenic animals and specific target protein-expressing animal cells. In addition, the clustered regularly interspaced short palindromic repeat-CRISPR associated protein 9 (CRISPR-Cas9) system have been utilized to generate chickens with knockout of G0/G1 switch gene 2 (G0S2) and myostatin, which are related to lipid deposition and muscle growth, respectively. These experimental chickens could be the invaluable genetic resources to investigate the regulatory pathways and mechanisms of improvement of economic traits such as fat quantity and growth. The gene-edited animals could also be applicable to the livestock industry.

Expression of a rice DREB1 gene, OsDREB1D, enhances cold and high-salt tolerance in transgenic Arabidopsis

  • Zhang, Yang;Chen, Chen;Jin, Xiao-Fen;Xiong, Ai-Sheng;Peng, Ri-He;Hong, Yi-Huan;Yao, Quan-Hong;Chen, Jian-Min
    • BMB Reports
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    • v.42 no.8
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    • pp.486-492
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    • 2009
  • OsDREB1D, a special DREB (dehydration responsive element binding protein) homologous gene, whose transcripts cannot be detected in rice (Oryza sativa L), either with or without stress treatments, was amplified from the rice genome DNA. The yeast one-hybrid assay revealed that OsDREB1D was able to form a complex with the dehydration responsive element/C-repeat motif. It can also bind with a sequence of LTRE (low temperature responsive element). To analyze the function of OsDREB1D, the gene was transformed and over-expressed in Arabidopsis thaliana cv. Columbia. Results indicated that the over-expression of OsDREB1D conferred cold and high-salt tolerance in transgenic plants, and that transgenic plants were also insensitive to ABA (abscisic acid). From these data, we deduced that this OsDREB1D gene functions similarly as other DREB transcription factors. The expression of OsDREB1D in rice may be controlled by a special mechanism for the redundancy of function.

Electromagnetic Forming Process Analysis Based on Coupled Simulations of Electromagnetic Analysis and Structural Analysis

  • Lee, Man Gi;Lee, Seung Hwan;Kim, Sunwoo;Kim, Jin Ho
    • Journal of Magnetics
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    • v.21 no.2
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    • pp.215-221
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    • 2016
  • We conducted a phased electromagnetic forming process analysis (EFPA) over time through a coupling of electromagnetic analysis and structural analysis. The analysis is conducted through a direct linkage between electromagnetic analysis and structural analysis. The analysis process is repeated until the electric current is completely discharged by a formed coil. We calculate the forming force that affects the workpiece using MAXWELL, a commercial electromagnetic finite element analysis program. Then, we simulate plastic behavior by using the calculated forming force data as the forming force input to ANSYS, a commercial structure finite element analysis program. We calculate the forming force data by using the model shape in MAXWELL, a commercial electromagnetic finite element analysis program. We repeat the process until the current is fully discharged by the formed coil. Our results can be used to reduce the error in data transformation with a reduced number of data transformations, because the proposed approach directly links the electromagnetic analysis and the structural analysis after removing the step of the numerical analysis of a graph describing the forming force, unlike the existing electromagnetic forming process. Second, it is possible to simulate a more realistic forming force by keeping a certain distance between nodes using the re-mesh function during the repeated analysis until the current is completely discharged by the formed coil, based on the MAXWELL results. We compare and review the results of the EFPA using the peak value of the forming force that acts on the workpiece (which is the existing analysis method), and the proposed phased EFPA over time approach.

Molecular Analysis of KP Elements Derived from Korean Populations of Drosophifa mefanogaster (Drosophila melanogaster 한국지반에서 분리한 KP Elements의 DNA 염기서열 분석)

  • Kim, Wook;Kim, Jeong-Soo;Shin, Dong-Jik
    • The Korean Journal of Zoology
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    • v.39 no.2
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    • pp.164-172
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    • 1996
  • KP elements derived from Korean populations (Seoul, Cheonan and Taegu) of Drosophila melunoguster were examined for their molecular structure. The entire 1.15 kb sequence of the three KP elements KC-137 (Cheonan), KS-95 (Seoul) and KT-99 (Taegu) have been obtained by PCR amplification using inverted repeat primers and DNA sequencing. The 1.15 kb fragments of KP elements were cloned into pCRmll vector plasmids, and subsequently sequenced. The sequence of the three KP elements in these populations suggested that there might have been derived from the complete P element by a 1753 bp internal deletion between positions 808 and 2560. Therefore these KP elements were confirmed to be identical to that isolated from M'10 strains widely distributed in most Eurasian populations of D. melanoguster. Sequence comparison with the 2.9 kb complete P element in pn25.1 revealed that KC-137 has only shown to be two base substitutions of A to G and G to A at positions 62 and 242, respectively. The retained sequence of the two KP elements KS-95 and KT-99 shows complete homology to the P factor in pn25.1. Based on this result, the two base substitutions in KC-137 might be due to Taq DNA pollunerase errors. Finally, it is suggested that the high copy numbers of KP elements provieds an explanation for the suppression of P-mediated hybrid dvssenesis in Korean population of D. melunogoster.

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