• Computers and Concrete
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• v.16 no.4
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• pp.531-544
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• 2015

The objective of this study was to examine the effect of repair mortar on the dynamic properties such as natural frequencies, mode shape and damping ratios of two story single span scale reinforced concrete building. To this end, two story single span scale reinforced concrete building having dimensions of 150 cm (width), 150 cm (length) and 135 cm (height) was constructed. Workmanship defects such as separation of material, faulty vibration application and bad gradation of the structure were properly evaluated. Dynamic properties of damaged structure were experimentally determined using Operational Modal Analysis (OMA). Detected defects in the structure were fixed by plastering with repair mortar. Dynamic properties of repaired structure were reevaluated by using the OMA method. Finite element software called Abaqus was used to numerically determine dynamic properties of the structure. Structure modeled as solid was subjected to Linear Perturbation Frequency Method. The changes in dynamic properties of structure after the repair process were comparatively studied by evaluating experimental and numerical results.

• BMB Reports
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• v.56 no.5
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• pp.265-274
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• 2023

Defects in DNA double-strand break (DSB) repair signaling permit cancer cells to accumulate genomic alterations that confer their aggressive phenotype. Nevertheless, tumors depend on residual DNA repair abilities to survive the DNA damage induced by genotoxic stress. This is why only isolated DNA repair signaling is inactivated in cancer cells. DNA DSB repair signaling contributes to general mechanism for various types of lesions in diverse cell cycle phases. DNA DSB repair genes are frequently mutated and amplified in cancer; however, limited data exist regarding the overall genomic prospect and functional result of these modifications. We list the DNA repair genes and related E3 ligases. Mutation and expression frequencies of these genes were analyzed in COSMIC and TCGA. The 11 genes with a high frequency of mutation differed between cancers, and mutations in many DNA DSB repair E3 ligase genes were related to a higher total mutation burden. DNA DSB repair E3 ligase genes are involved in tumor suppressive or oncogenic functions, such as RNF168 and FBXW7, by assisting the functionality of these genomic alterations. DNA damage response-related E3 ligases, such as RNF168, FBXW7, and HERC2, were generated with more than 10% mutation in several cancer cells. This study provides a broad list of candidate genes as potential biomarkers for genomic instability and novel therapeutic targets in cancer. As a DSB related proteins considerably appear the possibilities for targeting DNA repair defective tumors or hyperactive DNA repair tumors. Based on recent research, we describe the relationship between unstable DSB repairs and DSB-related E3 ligases.

• Journal of Microbiology
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• v.39 no.2
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• pp.102-108
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• 2001

DNA damage response has a central role in the maintenance of genomic integrity while mutations in related genes may result in a range of disorders including neoplasic formations. The uvsZl characterized in this report is a navel uvs mutation in Aspergillus nidulans, resulting in a nucleotide excision repair (NER) phenotype: UV-sensitivity before DNA synthesis (quiescent cells), high UV-induced mutation frequency and probable absence of involvement with mitotic and meiotic recombinations. The mutation is recessive and nan-allelic to the previously characterized uvsA101 mutation, also located on the paba-y interval on chromosome I. uvsZl skewed wild-type sensitivity to MMS, which suggests non-involvement of this mutation with BER. Epitasis tests showed that the uvsZ gene product is probably involved in the same repair pathways as UVSB or UVSH proteins. Although mutations in these proteins result in an NER phenotype, UVSB is related with cell cycle control and UVSH is associated with the post-replicational repair pathway. The epistatic interaction among uvsZl and uvsB413 and uvsH77 mutations indicates that different repair systems may be related with the common steps of DNA damage response in Aspergillus nidulans.

• Toxicological Research
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• v.34 no.4
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• pp.297-302
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• 2018

Cells are constantly exposed to endogenous and exogenous chemical and physical agents that damage their genome by forming DNA lesions. These lesions interfere with the normal functions of DNA such as transcription and replication, and need to be either repaired or tolerated. DNA lesions are accurately removed via various repair pathways. In contrast, tolerance mechanisms do not remove lesions but only allow replication to proceed despite the presence of unrepaired lesions. Cells possess two major tolerance strategies, namely translesion synthesis (TLS), which is an error-prone strategy and an accurate strategy based on homologous recombination (homology-dependent gap repair [HDGR]). Thus, the mutation frequency reflects the relative extent to which the two tolerance pathways operate in vivo. In the present paper, we review the present understanding of the mechanisms of TLS and HDGR and propose a novel and comprehensive view of the way both strategies interact and are regulated in vivo.

• Journal of Photoscience
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• v.9 no.2
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• pp.182-185
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• 2002

There are 3 UV DNA repair endonuclease activities in mammalian cells that cleave UV -irradiated DNA. Interestingly, mammalian UV endonuclease III with MW of 26.7kD has a lyase activity on AP sites. It also cleaves the phosphodiester bond within a cyclobutane pyrimidine dimer. Genomic analysis of human repair endonuclease III gene revealed that this gene has 100% sequence identity with ribosomal protein S3 (rpS3). Therefore, rpS3 seems to function both in translation and in DNA repair. This gene of about 6.1 kb contains 6 introns and 7 exons, and the first and fifth introns of human rpS3 gene contain functional U15 small nucleolar (sno) RNAs which appear to be involved in ribosome assembly. It is to be noted that the column profile of the endonuclease activity of rpS3 appears to be altered in Xeroderma Pigmentosum (XP) group D cells compared to normal cells indicating that this protein is involved in XP disease as well. XP is a human disease characterized by high sensitivity of skin by UV- or sun-light irradiation and by high frequency of developing skin cancers. We also report here that rpS3 protein is involved in other cellular functions.

• Journal of the Korean Institute of Rural Architecture
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• v.12 no.4
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• pp.1-11
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• 2010

This study is a research examining reshaped pattern in the perspective of repairing parts and materials through repairing renovation of registered cultural properties constructed in modern times and results are as followings. Repairing construction of registered cultural properties of Jollanamdo according to parts is shown numerously in the order of roof, walls and windows, and mostly outworn as time passes on and leakage were the main cause of repair. Also when original shape was damaged by previous repair in the past, and this was another reason of repairing. It was surveyed that among the repairing job repair of damage occurred on the part of roofing area including water leakage, corrosion and damage of roofing material, and damage of groove channel were the most main cause of repair. Especially when roof leakage is occurred by outworn of roofing materials, this cause corrosion and damage of materials due to the damage of leakage parts and this cause repeating cycles of worse leakage again and again. Main repairing materials which deform the original shape of registered cultural properties were confirmed as copper plate used on the roof. Copper plate showing high frequency of application which replaces groove slate or cement roofing tile used on the roof before has high durability and anti-corrosion but it is considered improper material to recover original shape.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6469-6474
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• 2012

Background: DNA repair is one of the crucial defense mechanism against mutagenic exposure. Inherited SNPs of DNA repair genes may contribute to variation in DNA repair capacity and susceptibility to cancer. Due to the presence of these variants, inter-individual and ethnic differences in DNA repair capacity have been established in various populations. India harbors enormous genetic and cultural diversity. Materials and Methods: In the present study we aimed to determine the genotypes and allele frequencies of XRCC1 Arg399Gln (rs25487), XRCC3 Thr241Met (rs861539), XPD Lys751Gln (rs13181), and OGG1 Ser326Cys (rs1052133) gene polymorphisms in 186 healthy individuals residing in the Hyderabad region of India and to compare them with HapMap and other populations. Results and Conclusions: The genotype and allele frequency distribution at the four DNA repair gene loci among Hyderabad population of India revealed a characteristic pattern. Comparison of these gene polymorphisms with other populations revealed a distinctiveness of Hyderabad population from the Deccan region of India. To the best of our knowledge, this is the first report of such DNA repair gene polymorphisms in the Deccan Indian population.

• International Journal of Highway Engineering
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• v.20 no.2
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• pp.9-17
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• 2018

PURPOSES : The frequency and severity of natural disasters such as torrential rain or typhoons have become increasingly significant worldwide. Events such as summer typhoons and localized torrential downpour can cause severe damages to a residential area and road networks, resulting in serious harm to the daily lives of people, especially in rural areas by isolating residents from road networks. An immediate and emergency repair technology for the collapsed road networks is urgently needed. This study introduces a new technology to repair road bases or slopes. METHODS : The development of new technology for emergency and permanent repair consists of first, packing of cement paste-coated gravel, second, combining appropriate equipment, and third, conducting a field applicability test. In this research, the compressive strength of cement pastecoated gravel, gravel-netting concrete properties, and packing efficiency were determined, and a full scale field mock-up test was carried out. RESULTS : The compressive strength of the cement paste-coated gravel concrete satisfied the required limit for road base of 5 MPa after 7 days. With appropriate netting materials and packing size, gravel-netting concrete was successful up to a slope of 1:1.5. The full scale field mock-up test showed efficiency in the field and penetration resistance performance. CONCLUSIONS : The new technology of emergency and permanent repair for damaged road bases and slopes, introduced in this study, showed satisfactory performance. The technology is expected to be applied in the field when construction procedures and quality specifications are made.

• Clinics in Shoulder and Elbow
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• v.26 no.2
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• pp.109-116
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• 2023

Background: Golf is a popular sport involving overhead activity and engagement of the rotator cuff (RC). This study aimed to determine to what level golfers were able to return to golf following RC repair, the barriers to them returning to golf and factors associated with their failure to return to golf. Methods: Patients preoperatively identifying as golfers undergoing RC repair at the study centre from 2012 to 2020 were retrospectively followed up with to assess their golf-playing status, performance and frequency of play and functional and quality of life (QoL) outcomes. Results: Forty-seven golfers (40 men [85.1%] and 7 women [14.9%]) with a mean age of 56.8 years met the inclusion criteria, and 80.1% were followed up with at a mean of 27.1 months postoperatively. Twenty-nine patients (76.3%) had returned to golf with a mean handicap change of +1.0 (P=0.291). Golf frequency decreased from a mean of 1.8 rounds per week preinjury to 1.5 rounds per week postoperatively (P=0.052). The EuroQol 5-dimension 5-level (EQ-5D-5L) index and visual analog scale (EQ-VAS) score were significantly greater in those returning to golf (P=0.024 and P=0.002), although functional outcome measures were not significantly different. The primary barriers to return were ipsilateral shoulder dysfunction (78%) and loss of the habit of play (22%). Conclusions: Golfers were likely (76%) to return to golf following RC repair, including mostly to their premorbid performance level with little residual symptomatology. Return to golf was associated with a greater QoL. Persistent subjective shoulder dysfunction (78%) was the most common barrier to returning to golf.

• Journal of the Korea institute for structural maintenance and inspection
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• v.22 no.1
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• pp.32-39
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• 2018

Repairing timing and the extended service life with repairing are very important for cost estimation during operation. Conventionally used model for repair cost shows a step-shaped cost elevation without consideration of variability of extended service life due to repairing. In the work, RC(Reinforced Concrete) Column is considered for probabilistic evaluation of repairing number and cost. Two mix proportions are prepared and chloride behavior is evaluated with quantitative exterior conditions. The repairing frequency and cost are investigated with varying service life and the extended service life with repairing which were derived from the chloride behavior analysis. The effect of COV(Coefficient of Variation) on repairing frequency is small but the 1st repairing timing is shown to be major parameter. The probabilistic model for repairing cost is capable of reducing the number of repairing with changing the intended service life unlike deterministic model of repairing cost since it can provide continuous repair cost with time.