• Advances in biomechanics and applications
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• 제1권3호
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• pp.169-185
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• 2014

Computational multibody models of the elbow can provide a versatile tool to study joint mechanics, cartilage loading, ligament function and the effects of joint trauma and orthopaedic repair. An efficiently developed computational model can assist surgeons and other investigators in the design and evaluation of treatments for elbow injuries, and contribute to improvements in patient care. The purpose of this study was to develop an anatomically correct elbow joint model and validate the model against experimental data. The elbow model was constrained by multiple bundles of non-linear ligaments, three-dimensional deformable contacts between articulating geometries, and applied external loads. The developed anatomical computational models of the joint can then be incorporated into neuro-musculoskeletal models within a multibody framework. In the approach presented here, volume images of two cadaver elbows were generated by computed tomography (CT) and one elbow by magnetic resonance imaging (MRI) to construct the three-dimensional bone geometries for the model. The ligaments and triceps tendon were represented with non-linear spring-damper elements as a function of stiffness, ligament length and ligament zero-load length. Articular cartilage was represented as uniform thickness solids that allowed prediction of compliant contact forces. As a final step, the subject specific model was validated by comparing predicted kinematics and triceps tendon forces to experimentally obtained data of the identically loaded cadaver elbow. The maximum root mean square (RMS) error between the predicted and measured kinematics during the complete testing cycle was 4.9 mm medial-lateral translational of the radius relative to the humerus (for Specimen 2 in this study) and 5.30 internal-external rotation of the radius relative to the humerus (for Specimen 3 in this study). The maximum RMS error for triceps tendon force was 7.6 N (for Specimen 3).

• 한국건설관리학회논문집
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• 제11권5호
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• pp.53-64
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• 2010

리모델링은 기존 건물을 완전 철거하지 않고 구조체의 일부만을 철거하여 자원의 재활용 이라는 측면에서 매우 중요한 이슈가 되고 있다. 그러나 리모델링의 경우에 신축과 달리 철거와 보수보강 공사가 추가되고, 다수의 발주자 조직으로 인한 의사결정에 어려움이 발생하고 있다. 이러한 문제를 해결하기 위한 방안으로 건물의 라이프사이클 동안에 발생하는 정보를 생산하고 관리하는 통합 도구로서 BIM이 해결 방안으로서 가능성을 가지고 있다. 그러나 현재 BIM의 경우 신축 공사시 3D 모델을 통한 간섭체크나 디자인 대안 결정에 주로 활용하고 있으며, 공사비 산출 등을 위한 BIM 데이터베이스의 경우 신축사의 BIM 적용을 위한 프레임웍과 BIM 데이터베이스를 제시하고 사례적용을 통하여 리모델링 공사를 위한 BIM 데이터베이스의 적용 가능성을 검증하고자 하였다.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권4호
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• pp.308-313
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• 2011

Purpose: Beta-tricalcium phosphate (${\beta}$-TCP) is a synthetic calcium phosphate ceramic that has widely been used as a bone material to repair bone defects. Despite many clinical studies, the molecular mechanism whereby this biomaterial alters the gene expression in osteoblasts to promote bone formation is poorly understood. Thus, we attempted to address this question by using microarray techniques to identify the genes that are differentially regulated in osteoblasts exposed to ${\beta}$-TCP. Methods: By using DNA microarrays, we identified several genes whose expression levels were significantly up- or down-regulated in osteoblast-likeMG-63cells cultured with ${\beta}$-TCP at a concentration of 100 mg/10 ml for 24 hours. Results: The differentially expressed genes covered a broad range of functional activities: signal transduction, transcription, cell cycle regulation, vesicular transport, apoptosis, immunity, cytoskeletal elements and cell proliferation and differentiation. Conclusion: The gene expression changes related to cell proliferation and differentiation, vesicle transport, immunity and defense could affect the osteogenic activities of osteoblasts for bone regeneration. However, further studies will be required to verify the relative importance of these genes in bone formation, their temporal and spatial expression patterns and their interactions with each other.

• Molecular & Cellular Toxicology
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• 제2권2호
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• pp.75-80
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• 2006

Biomarkers identify various stages and interactions on the pathway from exposure to disease. The three categories of biomarkers are those measuring susceptibility, exposure and effect. Susceptibility biomarkers are identifiable genetic variations affecting absorption, metabolism or response to environmental agents. Biomarkers of exposure indicate the amount of a foreign compound that is absorbed into the body. Biological measurements performed on human tissues are vastly expanding the capabilities of classical epidemiology, which has relied primarily on estimates of human exposure derived form chemical levels in the air, water, and other exposure routes. Biomarkers of exposure indicate the amount of a foreign compound that is absorbed into the body. Biological measurements performed on human tissues are vastly expanding the capabilities of classical epidemiology, which has relied primarily on estimates of human exposure derived form chemical levels in the air, water, and other exposure routes. The biomarker response is typical of chemical pollution by specific classes of compound, such as (i) heavy metals (mercury, cadmium, lead, zinc), responsible for the induction of metallothionein synthesis, and (ii) organochlorinated pollutants (PCBs, dioxins, DDT congeners) and polycyclic aromatic hydrocarbons (PAHs), which induce the mixed function oxygenase (MFO) involved in their bio transformations and elimination. Currently genomic researches are developed in human cDNA clone subarrays oriented toward the expression of genes involved in responses to xenobiotic metabolizing enzymes, cell cycle components, oncogenes, tumor suppressor genes, DNA repair genes, estrogen-responsive genes, oxidative stress genes, and genes known to be involved in apoptotic cell death. Several research laboratories in Korea for kicking off these Environmental Genomics were summarized.

• Asian Pacific Journal of Cancer Prevention
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• 제15권17호
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• pp.7413-7417
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• 2014

Background: Development of gastric cancer (GC) is a multistep process that requires alterations in the expression of oncogenes and tumor suppressor genes, occurring over several decades. The p53 tumor suppressor protein is involved in cell-cycle control, apoptosis and DNA repair. One of the most important regulators of p53 is MDM2, which acts as a negative regulator in the p53 pathway. Based on the key role of p53 and MDM2 in tumor suppression, polymorphisms that cause change in their function might affect cancer risk. We therefore elevated associations of the polymorphisms of p53 (R72P) and MDM2 (SNP309) with GC in Iran. Materials and Methods: A total of 104 patients with gastric cancer and 100 controls were recruited. Genomic DNA was extracted from fresh gastric samples. Genotyping of the p53 and MDM2 genes was performed using allele specific PCR (AS-PCR). Results: There was no significant difference between the p53 codon 72 polymorphism distribution in control and patient groups (p=0.54), but the G allele of MDM2 was found to be over-represented in patients (p=0. 01, Odds Ratio=2. 08, 95% Confidence Interval= 1.37-4.34). Conclusions: The p53 R72P seems not to be a potential risk factor for development of GC among Iranian patients, but our data suggest that MDM2 SNP309 might modify the risk related to GC.

• 한국재난관리표준학회지
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• 제2권4호
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• pp.35-41
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• 2009

교량 상시계측시스템을 이용하여 교량을 감지할 경우 지속적인 교량의 거동이력을 확인할 수 있어 손상의 진행을 관리자가 손쉽게 확인할 수 있으며, 지진이나 홍수와 같은 대규모의 재난상황이 발생할 경우 교량의 상태변화를 정량적으로 파악하여 교량 통제 및 통행제한 등 적절한 사전 조치 등 취할 수 있다. 또한 보수 및 보강 등의 교량 성능향상 작업시점을 적절하게 판단할 수 있어 생애주기를 통한 교량 유지관리 비용을 절감할 수 있다. 교량 상시계측시스템에 적용되고 있는 다양한 계측시스템의 계측항목별 관리 기준치 설정방법 중 범용 유한요소 해석프로그램을 이용하여 교량의 관리기준치를 설정하는 방법을 분석하고 강상자형교에 대한 계측항목별 관리기준을 설정하여 유한요소 해석프로그램을 통한 강상자형교 상시계측시스템 계측항목별 관리기준치 설정의 기준을 제시하였다.

• Asian Pacific Journal of Cancer Prevention
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• 제17권7호
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• pp.3623-3626
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• 2016

Breast cancer is very common and the leading cause of cancer deaths among women globally. Hereditary cases account for 5-10% of the total burden and CHEK2, which plays crucial role in response to DNA damage to promote cell cycle arrest and repair or induce apoptosis, is considered as a moderate penetrance breast cancer risk gene. Our objective in the current study was to analyze mutations in related to breast cancer. A total of 271 individuals including breast cancer patients and normal subjects were enrolled and all 14 exons of CHEK2 were amplified and sequenced. The majority of the patients (>95%) were affected with invasive ductal carcinoma (IDC), 52.1% were diagnosed with grade III tumors and 56.2% and 27.5% with advanced stages III and IV. Two novel nonsense variants i.e. c.58C>T (P.Q20X) and c.256G>T (p.E85X) at exon 1 and 2 in two breast cancer patients were identified, both novel and not reported elsewhere.

• 대한기계학회논문집A
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• 제34권9호
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• pp.1325-1330
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• 2010

터빈설비 각 중요부품의 고장은 발전정지라는 큰 파급효과를 유발하며, 예기치 못한 고장으로 설비의 이용률이 감소하게 되면 막대한 경제적 손실이 발생한다. 현재 발전설비는 제작사에서 제시한 정비주기를 기준으로 보수적인 예방정비를 실시하고 있으나, 급변하는 경영환경에서 경쟁력을 유지하기 위해서는 신뢰도를 유지하면서 정비비용을 절감하는 신뢰도 기반 정비방법을 도입 해야 할 필요가 있다. 신뢰성 있는 정비주기를 선정하기 위해서는 설비의 고장이력에 대한 면밀한 분석을 통하여 고장확률을 예측해야 한다. 본 논문은 발전설비 중 터빈 각 부품들의 고장이력을 데이터베이스로 만들고, Weibull 함수를 이용하여 최적의 정비시점을 예측하며, 정비주기를 결정하는 방법에 대하여 연구하였다.

• 한국약용작물학회지
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• 제21권2호
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• pp.136-141
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• 2013

Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various biological activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has been reported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study, we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processes such as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regulated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level of FOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 and GADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxidant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggest that CM extract has an antioxidant activity through FOXO activation.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2106-2115
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• 2016

To identify the global effects of (p)ppGpp in the gram-positive bacterium Deinococcus radiodurans, which exhibits remarkable resistance to radiation and other stresses, RelA/SpoT homolog (RSHs) mutants were constructed by direct deletion mutagenesis. The results showed that RelA has both synthesis and hydrolysis domains of (p)ppGpp, whereas RelQ only synthesizes (p)ppGpp in D. radiodurans. The growth assay for mutants and complementation analysis revealed that deletion of relA and relQ sensitized the cells to $H_2O_2$, heat shock, and amino acid limitation. Comparative proteomic analysis revealed that the bifunctional RelA is involved in DNA repair, molecular chaperone functions, transcription, the tricarboxylic acid cycle, and metabolism, suggesting that relA maintains the cellular (p)ppGpp levels and plays a crucial role in oxidative resistance in D. radiodurans. The D. radiodurans relA and relQ genes are responsible for (p)ppGpp synthesis/hydrolysis and (p)ppGpp hydrolysis, respectively. (p)ppGpp integrates a general stress response with a targeted re-programming of gene regulation to allow bacteria to respond appropriately towards heat shock, oxidative stress, and starvation. This is the first identification of RelA and RelQ involvement in response to oxidative, heat shock, and starvation stresses in D. radiodurans, which further elucidates the remarkable resistance of this bacterium to stresses.