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http://dx.doi.org/10.7783/KJMCS.2013.21.2.136

Antioxidant Activity and Its Mechanism of Chelidonium majus Extract  

Heo, Jee In (Department of Biochemistry, Hallym University Medical School)
Kim, Jeong Hyeon (Department of Biochemistry, Hallym University Medical School)
Lee, Jeong Min (Department of Biochemistry, Hallym University Medical School)
Lim, Soon Sung (Institute of Natural Medicine, Hallym University Medical School)
Kim, Sung Chan (Department of Biochemistry, Hallym University Medical School)
Park, Jae Bong (Department of Biochemistry, Hallym University Medical School)
Kim, Jae Bong (Department of Biochemistry, Hallym University Medical School)
Lee, Jae Yong (Department of Biochemistry, Hallym University Medical School)
Publication Information
Korean Journal of Medicinal Crop Science / v.21, no.2, 2013 , pp. 136-141 More about this Journal
Abstract
Chelidonium majus (CM) contains several isoquinoline alkaloids that have been reported to have various biological activities such as anti-inflammatory, antimicrobial, antioxidant, immune-modulatory, and antitumoral. It has been reported that the extract of CM had an antioxidant potential, however the mechanism has not been verified. In this study, we found that CM extract activated FOXO3a. FOXO3a is a transcription factor that involved in various biological processes such as cell cycle arrest, apoptosis, DNA repair, and ROS detoxification. Transcriptional activities of FOXO3a were regulated by post-translational modifications including phosphorylation, acetylation, and ubiquitination. Protein level of FOXO3a was increased by CM extract. Promoter activities of FOXO-transcriptional target genes such as MnSOD, p27 and GADD45 were activated by CM extract in a dose dependent manner. In addition, protein level of MnSOD, major antioxidant enzyme, was increased by CM extract. Thereby ROS level was decreased by CM in old HEF cells. These results suggest that CM extract has an antioxidant activity through FOXO activation.
Keywords
Chelidonium majus; FOXO3a; Antioxidant;
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