• Journal of Chest Surgery
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• v.28 no.8
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• pp.742-746
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• 1995

The present study was aimed at investigating possible transmitter mechanisms in the endothelial cell layer in regulating the tone of the vascular smooth muscle. The thoracic aorta was isolated from the anesthetized male white rabbits and its helical strips were prepared. Electrical field stimulation was delivered to platinum wire electrodes positioned parallel to the vessel segment preconstricted with phenylephrine [3.5x10-6 mol/L at a distance of 1.5-2.0 mm. The electrical stimulation [70 V, 5 msec, 0.5-200 Hz caused either relaxation only [34% or a biphasic response [prolonged relaxation following a weak and transient contraction, 66% . The relaxation response was frequency- dependent, and at 200 Hz a complete relaxation was noted. Mechanical rubbing of the endothelial layer abolished or greatly attenuated the relaxation. The relaxation was also markedly attenuated in the presence of NG-nitro- L-arginine methyl ester [10-3mol/L or procaine hydrochloride [3.5x10-4mol/L . Tetrodotoxin,guanethidine, atropine or indomethacin failed to block or enhance the relaxation response to electrical field stimulation. It is concluded that the vascular endothelium in the aorta contains diffusible substances that regulates the function of the smooth muscle layer, in which relaxation is more prominent than contraction. Their release by the electrical stimualtion in vitro may not involve classic neuronal transmitter release mechanisms or metabolism of arachidonic acids by cyclooxygenase. The release of the relaxing agents may require an increase in cytosolic calcium level. The chemical nature of the relaxant may be, to a large extent, nitric oxide.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.1
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• pp.63-68
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• 1998

Nitric oxide (NO)-mediated relaxation in vascular smooth muscle involves not only activation of guanylate cyclase but also hyperpolarization of the membrane. It has been shown that depolarization decreases the [$Ca^{2+}$] sensitivity of myosin light chain kinase in arterial smooth muscle, and nitric oxide (NO)-mediated relaxation was attenuated in this situation. However, why potassium inhibits or attenuates the action of EDRF/NO is not clear. Therefore, we investigated the magnitude of relaxation and cGMP contents using measures known to release NO, such as photorelaxation, photo activated NO-mediated relaxation, and NO-donor (SNP)-mediated relaxation in porcine coronary arterial rings in which contractile conditions were made by different degree of depolarization, i.e., contraction in response to U46619 or U46619 plus KCl. In all cases, the magnitude of relaxation was significantly greater (P＜0.05) in U46619-contracted rings than in U46619+KCl-contracted ones. Although accumulation of cGMP was evident with three measures employed in the present study, no difference was found in cGMP contents between U46619 and U46619+KCl conditions, indicating that the diminished relaxation in KCl containing solution is cGMP-independent mechanism(s). To understand this further, cytosolic $Ca^{2+}$ changes due to NO were compared in rat thoracic aorta by exploiting photoactivated NO using streptozotocin (STZ) that was contracted with either NE or KCl. Fura-3 $[Ca]_{cyt}$ signal caused by NO was small and transient in high $K^+$-, but large and sustained in NE-contracted aorta. The inhibitory potency of STZ expressed in terms of $IC_{50}$ was 5.14 and 3.88 ${\mu}M$ in NE and in high $K^+$, respectively. These results suggest that modification of the cellular mobilization of $Ca^{2+}$ rather than cGMP levels may be an important mechanism for the NO-mediated relaxation when vascular membrane is depolarized, such as atherosclerosis and hypertension.

• Steel and Composite Structures
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• v.16 no.6
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• pp.703-718
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• 2014

The initial clamping forces of high strength bolts subjected to different faying surface conditions drop within 500 hours regardless of loading, any other external force or loosening of the nut. This study develops a mathematical model for relaxation confined to creep on a coated faying surface after initial clamping. The quantitative model for estimating relaxation was derived from a regression analysis for the relation between the creep strain of the coated surface and the elapsed time for 744 hours. This study establishes an expected model for estimating the relaxation of bolted joints with diverse coated surfaces. The candidate bolts are dacro-coated tension control bolts, ASTM A490 bolt, and plain tension control bolts. The test parameters were coating thickness, species of coating. As for 96, 128, 168, and $226{\mu}m$ thick inorganic zinc, when the coating thickness was increased, relaxation after the initial clamping rose to a much higher range from 10% to 18% due to creep of the coating. The amount of relaxation up to 7 days exceeded 85% of the entire relaxation. From this result, the equation for creep strain can be derived from a statistical regression analysis. Based on the acquired creep behavior, it is expected that the clamping force reflecting relaxation after the elapse of constant time can be calculated from the initial clamping force. The manufacturer's recommendation of inorganic zinc on faying surface as $75{\mu}m$, appears to be reasonable.

• Archives of Pharmacal Research
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• v.29 no.5
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• pp.400-404
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• 2006

This study examined the effect of Gamma-Amino butyric acid (GABA) and selective GABA receptor related drugs on the electrically stimulated relaxation in the lower esophageal sphincter muscle (LES) of a cat. Tetrodotoxin $(10^{-6}\;M)$ suppressed the electrically stimulated (0.5-5 Hz) relaxation of the LES. However, guanethidine $(10^{-6}\;M)$ and atropine $(10^{-6}\;M)$ had no effect indicating that the relaxations were neurally mediated via the nonadrenergic and noncholinergic (NANC) pathways. NG-nitro-L-arginine methyl ester ($10^{-4}M$, L-NAME) also inhibited the relaxant response but did not completely abolish the electrically stimulated relaxation with 60% inhibition, which suggests the involvement of nitric oxide as an inhibitory transmitter. This study examined the role of GABA, an inhibitory neurotransmitter, on neurally mediated LES relaxation. GABA ($10^{-3}-10^{-5}M$, non selective receptor agonist), muscimol ($10^{-3}-10^{-5}M$, GABA-A agonist), and baclofen ($10^{-3}-10^{-5}M$, GABA-B agonist) had no significant effect on the electrically stimulated relaxation. Moreover, bicuculline ($10^{-5}M$, GABA-A antagonist) and phaclofen ($10^{-5}M$, GABA-B antagonist) had no inhibitory effect on the electrically stimulated relaxation. This suggests that GABA and the GABA receptor are not involved in the electrically stimulated NANC relaxation in the cat LES.

• Journal of Korean Academy of Nursing
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• v.19 no.2
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• pp.191-202
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• 1989

This study was peformed to examine the effect of muscle relaxation training on patients with insomnia complaint. The subjects were selected those who have taken poor sleep below 240-300 minutes. per day, between 16-15ages, without organic brain syndroms. Using the Budzinski's muscle relaxation training tapes the patients have been gotten the muscle relaxation training daily during four weeks and examined the state anxiety with Spielberger's Trait-State Anxiety Scale, and investigated Sleep amounts, Blood pressure, Pulse rates, Respiratory rates before and after the muscle relaxation training. The results were as follow : 1. The mean of the State anxiety after the muscle relaxation training(41.8$\pm$6.4) was significantly lower than that of before training (54.2$\pm$7.0) (p＜0.001). 2. The mean of systolic blood pressure after the training (114.$\pm$7.8mmHg) was significantly lower than that of before training (139.0$\pm$9.8) (P＜0.001). 3. The mean of Pulse rate after the traing (89.2$\pm$3.0) was significantly lower than that of before training (103.9$\pm$7.4) (P＜0.001). 4. The mean of Respiratory rate after the training(18.6$\pm$1.0) was significantly lower than that of before training(22.8$\pm$1.3) (P＜0.001). 5. The mean of Sleep amount after the muscle relaxation training (459.8$\pm$52.4 minutes) was significantly increased than that of before training (287.3$\pm$30.3) (P＜0.001).

• Journal of Korean Biological Nursing Science
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• v.12 no.1
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• pp.8-15
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• 2010

Purpose: The purpose of this study is to investigate the effects of relaxation on stress and blood pressure of the college students induced by a cognitive distress game. Methods: The subjects for this research were 18 individuals whose stress index scores were below 4.0 (Asan city). The 18 subjects were divided into two, 9 students were assigned to the experimental group and the other 9 students to the control group randomly. Survey was used to gather data to recognize general characteristics and the level of stress and blood pressure were measured before and after cognitive distress game and relaxation. The gathered data were analyzed with frequency, $X^2$-test, Wilcoxon signed ranks test and Mann Whitney U test using SPSS 14.0 program. Results: Both level of stress and blood pressure displayed a statistically significant difference pre and post cognitive distress game. Relaxation had a statistically significant effect on level of stress. Relaxation, however, did not have statistically significant effect on blood pressure. Conclusion: The above results suggest that while relaxation was effective in reducing level of stress for college students, and therefore recommendable as a means to deal with stress. However, to investigate the effects on physiological index such as blood pressure, further research is needed.

• The Korean Journal of Physiology and Pharmacology
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• v.2 no.2
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• pp.217-223
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• 1998

Oxygen-derived free radicals have been implicated in many important functions in the biological system. Electrical field stimulation (EFS) causes arterial relaxation in animal models. We found that EFS applied to neither muscle nor nerve but to Krebs solution caused a relaxation of rat aorta that had been contracted with phenylephrine. In the present study, therefore, we investigated the characteristics of this EIRF (electrolysis-induced relaxing factor) using rat isolated aorta. Results indicated that EIRF acts irrespective of the presence of endothelium. EIRF shows positive Griess reaction and is diffusible and quite stable. EIRF-induced relaxation was stronger on PE-contracted aorta than on KCl-contracted one, and inhibited by the pretreatment with methylene blue. Zaprinast, a cGMP-specific phosphodiesterase inhibitor, potentiated the EIRF-induced relaxation. $N^G-nitro-L-arginine$, NO synthase inhibitor, did not inhibit the EIRF-induced relaxation. Deferroxamine, but not ascorbic acid, DMSO potentiated the EIRF-induced relaxation. These results indicate that electrolysis of Krebs solution produces a factor that relaxes vascular smooth muscle via cGMP-mediated mechanism.

• Journal of the Korean Magnetic Resonance Society
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• v.18 no.1
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• pp.15-23
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• 2014

The structural properties and relaxation processes of $MCsSO_4$ (M = Na, K, or Rb) crystals were investigated by measuring the NMR spectra and spin-lattice relaxation rates $1/T_1$ of their $^{23}Na$, $^{39}K$, $^{87}Rb$, and $^{133}Cs$ nuclei. According to the NMR spectra, the $MCsSO_4$ crystals contain two crystallographically inequivalent sites each for the M and Cs ions. Further, the relaxation rates of all these nuclei do not change significantly over the investigated temperature range, indicating that no phase transitions occur in these crystals in this range. The variations in the $1/T_1$ values of the $^{23}Na$, $^{39}K$, $^{87}Rb$, and $^{133}Cs$ nuclei in these three crystals with increasing temperature are approximately proportional to $T^2$, indicating that Raman processes may be responsible for the relaxation. Therefore, for nuclear quadrupole relaxation of the $^{23}Na$, $^{39}K$, $^{87}Rb$, and $^{133}Cs$ nuclei, Raman processes with n = 2 are more effective than direct processes.

• Proceedings of the KWS Conference
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• 2003.11a
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• pp.216-218
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• 2003

Most of ferrous b.c.c weld materials may experience martensitic transformation during rapid cooling after welding. And it is well known that volume expansion due to phase transformation could influence in the case of welding of high tensile strength steels on the relaxation of welding residual stress. To apply this effect practically, it is a prerequisite to establish a numerical model which is able to estimate the effect of phase transformation on residual stress relaxation quantitatively. In this study, we investigated the effect of phase transformation on the relaxation of welding residual stress through experiment. And three-dimensional thermal elastic-plastic FEM analysis is conducted to compare the effect of phase transformation on the relaxation of welding residual stress in high strength steels(POSTEN60, POSTEN80) with analytical results which is not considering the effect of phase transformation on residual stress relaxation. According to the results, the extents of welding residual stress relaxation due to phase transformation in the case of welding of POSTEN60, POSTEN80 are 0.85 $\sigma$/$\sigma$$\sub$Y0/, 0.75$\sigma$/$\sigma$$\sub$Y0/, respectively.

• Journal of Korean Academy of Nursing
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• v.16 no.3
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• pp.123-130
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• 1986

This Study is aimed at showing the effect of relaxation technique on reduction of anxiety of nursing students in the first clinical practice. The samples of this research were eighty fresh-man students of Dong San Junior College of Nursing. Of them thirty-eight were experimental group and remaining forty-two were control group. This study has been conducted from October 14 to 22, 1985. Subjects in all two groups had measured their trait anxiety, after then, the researcher carried out Byun's relaxation technique with experimental group every other day After the experimental period, subjects in all two groups had measured their state anxiety. The tools of this study were two kinds: Spielberg, Gorsuch & Lushene's STAI, and Byun's relaxation technique. To test the statistical difference in the subjects' trait anxiety, score of lecture in pre-clinical practice and score of practice pre-clinical practice, t-test was performed. To verify the hypotheses, ANCOVA was performed. Results of this study were as follows: The main hypothesis that the nursing students who use relaxation technique will have less degree of anxiety in the first clinical practice than the nursing students who don't use relaxation technique was accepted (P<.005). Conclusively, the researcher thinks that it is necessary that nursing students were prepared with relaxation technique to reduce anxiety in their first clinical practice.