• Analytical Science and Technology
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• v.11 no.5
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• pp.374-385
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• 1998

Phytoestrogens are biologically active compounds derived from plants foods. It had been suggested that phytoestrogens, by inhibiting aromatase in peripheral and/or cancer cells and lowering estrogen levels, may play a protective role as antipromotional compounds during growth of estrogen-dependent cancers. Therefore, simultaneous analysis of estrogens and phytoestrogens is necessary to elucidate the possible involvement of phytoestrogens in estrogen metabolism. In this view, we developed a simple and reproducible procedure to quantitatively determine estrogen and phytoestrogen metabolites. The proposed method consisted of solid phase extraction using preconditioned Serdolit AD-2 resin, enzyme hydrolysis with ${\beta}$-glucuronidase/arylsulfatase from Helix pomatia, liquid-liquid extraction and TMS-ether derivatization. And the final determination was carried out by gas chromatography/mass spectrometry (GC/MS) in selected ion monitoring mode (SIM). The precision and accuracy of this method was evaluated through within-a-day and day-to-day test. Recovery range and detection limit were 71.96~105.66%, 2~4 ng/mL, respectively. Using this method, 17 estrogen and 5 phytoestrogen compositions in urine of normal subjects were analyzed. It was found that amounts and relative distribution of urinary phytoestrigens and estrogens showed different pattern in male and female subjects.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.7
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• pp.735-742
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• 2008

In order to evaluate the levels and distribution patterns, the concentrations of PAHs and chlorophenols were investigated in sludge samples discharged from 6 WWTPs located along Nak-dong river and 7 STPs in Busan, Korea. Levels of 16 PAHs and 19 chlorophenols in sludge samples ranged from 1.28 to 44.9 mg/kg dry wt. and from 213 to 3,850 $\mu$g/kg dry wt., respectively. Levels of PAHs in sludge samples except I5 and S4 were detected lower than those of previous studies. The distribution patterns of PAHs and chlorophenols varied with industrial wastewater sludge samples because industrial wastewater sludge had different industrial input sources. However, the distribution patterns of PAHs and chlorophenols in sewage sludge were pretty similar. Phenanthrene, fluoranthene and pyrene were dominant and the fractions of these 3 PAHs relative to 16 PAHs in sewage sludge ranged from 30.8 to 50.7%. 2-chlorophenol is dominated in most sewage sludge samples and the fraction ranged from 36.0 to 66.8%.

• The Korean Journal of Physiology
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• v.2 no.1
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• pp.31-38
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• 1968

Total body fat measurement by means of skinfold thickness was performed in 94 secondary school boys. Hydrostatic weighing was made on the same subject and corrected for residual volume in lung. Skinfold thicknesses at four sites, namely, back, waist, arm and abdomen, were compared with total body fat calculated from the equation given by Keys and Brozek and regression equation were computated. In 48 middle school boys aged between 13 and 16 years and 46 high school boys aged between 16 and 19 years, skinfold thicknesses increased at all 4 sites as age increased. The body density, however, showed different pattern. In middle school ages, body density showed little variation. Density in 13-14 years was 1.0608, in 14-15 years 1.0578 and in 15-16 years 1.0546. In high school ages density in-creased abruptly to 1.0703 (16 yr.), 1.0730 (17 yr.) and 1.0740 (18 yr.). Subsequently 15 years was a boundary for density change and total body fat change. In middle school boys as a group of 48 the observed values were: density: 1.0562, total body fat: 15.9%, mean skinfold thickness at four sites: 6.02 mm. The coefficient of correlation between mean skinfold thickness and density were r=-0.759 and r=0.781 with %fat. The regression equations were : Body $density=-0.00527{\times}Mean$ skinfold thickness (mm)+1.0879, %Fat=$1.933{\times}Mean$ skinfold thickness (mm)+4.26 In high school boys the values were: density: 1.0723, body fat: 10.4%, and mean skinfold thickness: 7.89 mm. Coefficients of correlation between mean skinfold thickness and density were r=-0.868 and r=0.855 with % fat. Thus, Body density=$-0.00365{\times}Mean$ skinfold thickness (mm)+1.1008, and %Fat=$1.326{\times}Mean$ skinfold thickness (mm)-0.064, were obtained. Although skinfold thicknesses at 4 sites showed a continuous increase in absolute value as age increased, relative growth of skinfold at specific site differed. On arm the growth of skinfold showed a decrease and on waist it showed an increase as compared to the mean thickness.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.3
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• pp.242-246
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• 2002

Growth and tetraspore release pattern of lithophyllum yessoense (Rhodophyta, Corallinaceae) were investigated from March 2000 to July 2001. Pinkish tetraspores were 40.2 $\pm$ 0.4 $\mu$m in diameter. After release, tetraspores attached on substrate shortly. Culture conditions were five temperatures (5, 10, 15, 20, 25^{\circ}C), four irradiances (0, 20, 50, 100 $\mu$mol${\cdot}m^{-2}$${\cdot}s^{-1}$) and six salinities (0, 9, 17, 25, 34, 43 ppt). Maximum growth of gametophyte was occurred at $20^{\circ}C$, $20{\mu}mol\;m^{-2}$${\cdot}s^{-1}$, 16: 8h (L:D) and 34 put. Maximum relative growth rate was 0.1232 at $20^{\circ}C$. The amount of tetraspore release showed maximum at September as 266 cells per crust area ($cm^2$), and tetraspores did not release from January to March.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.6
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• pp.427-434
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• 2008

Processing of collagen from yellowfin tuna (Thunnus albacares) abdominal skins was optimized by response surface methodology and central composite design. The values of independent variables at optimal conditions were NaOH concentration: 0.5 N, NaOH treatment time: 36.2 hr, pepsin concentration: 1:4.9 ratio (0.245%, w/v), and digestion time: 48.1 hr, respectively. The collagen content estimated under optimal conditions was 33.1%, and the actual experimental collagen content was 32.3%. Physicochemical properties of collagen from yellowfin tuna abdominal skin were investigated by amino acids analysis, SDS-PAGE, FT-IR, viscosity and denaturation temperature. Amino acids content of the collagen was 21.0%. SDS-PAGE pattern of the collagen showed two different $\alpha$-chain (${\alpha}_1$- and ${\alpha}_2$- chain), $\beta$-component and $\gamma$-component. The spectrum of FT-IR of the collagen showed wavenumber at 3,434, 1,650, 1,542 and $1,235\;cm^{-1}$ representing the regions of amide A, I, II and III, respectively. Relative viscosity of the collagen decreased continuously on heating up to $32^{\circ}C$, and the rate of decrease was retarded in the temperature range of $35-50^{\circ}C$. Denaturation temperature (Td) of the collagen solution (0.06%, w/v) was $31^{\circ}C$ and was lower than calf skin collagen ($35^{\circ}C$).

• Journal of the Korean Wood Science and Technology
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• v.41 no.1
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• pp.19-32
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• 2013

In this study, outstanding white rot fungi for biodegradation of organosolv lignin were selected on the basis of their ligninolytic enzyme system. Fifteen white rot fungi were evaluated for their ability to decolorize Remazol Brilliant Blue R (RBBR) in SSC and MEB medium, respectively. Six white rot fungi (Ceriporiopsis subvermispora, Ceriporia lacerate, Fomitopsis insularis, Phanerochaete chrysosporium, Polyporus brumalis, and Stereum hirsutum) decolorized RBBR rapidly in SSC medium within 3 days. The protein contents as well as the activities of manganese peroxidase (MnP) and laccase for 6 selected fungi were determined on the SSC medium with and without organosolv lignin. Interestingly, extracellular protein concentrations were determined to relative higher for S. hirsutum and P. chrysosporium in the presence of organosolv lignin than others. On the other hands, each fungus showed a different ligninolytic enzyme pattern. Among them, F. insularis resulted the highest ligninolytic enzyme activities on incubation day 6, indicating of 1,545 U/mg of MnP activity and 1,259 U/mg of laccase activity. In conclusion, $STH^*$ and FOI were considered as outstanding fungi for biodegradation of organosolv lignin, because $STH^*$ showed high extracellular protein contents and ligninolytic enzyme activities over all, and ligninolytic enzyme activities of FOI were the highest among white rot fungi used in this study.

• The korean journal of orthodontics
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• v.29 no.1 s.72
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• pp.23-35
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• 1999

Lateral cephalograms or 251 males md 286 females were taken and pubertal growth pattern or cranial base, maxillary and mandible of 7 to 17 years old Korean children was evaluated. 10 landmarks and 16 analytical measurements were evaluated. Analytical measurement and annual difference for each age group was calculated and tested for statistical significance. Analytical measurements were classified into three groups which were cranial base, maxillary and mandibular measurements and also classified into make and female measurements. Following results were achieved. 1. The circumpuberal growth spurt was earlier in Korean females than in males. 2. Cranial base, maxilla and mandible showed circumpuberal growth. The cranial base showed a relatively smaller amount of growth than the facial complex. 3. Middle and posterior cranial base length showed a floater increase than anterior cranial base length and circumpuberal growth spurt was also more definite. 4. the forward and downward growth or maxilla results from maxillary growht itself and transposition or the maxilla due to circumsutural growth aroud the maxilla. Ar-ANS and Ar-Pr which represent maxillary position relative to the cranial base showed more growth than ANS-PNS which represents maxillary bone growth. 5. mandible showed more vertical growth than horizontal growth but without significance. 6. Alveolar gwoth of maxilla and mandible show maximum growth rate of the time of permanent teeth eruption following loss of deciduous teeth . After this period alveolar growth shows a decreasing tendency.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.2
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• pp.128-139
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• 2017

Railway is one of the public transportation systems along with shipping and aviation. With the recent introduction of high speed train, its proportion is increasing rapidly, which results in the higher risk of catastrophic failures. The wheel bearing to support the train is one of the important components requiring higher reliability and safety in this aspect. Recently, many studies have been made under the name of prognostics and health management (PHM), for the purpose of fault diagnosis and failure prognosis of the bearing under operation. Among them, the most important step is to extract a feature that represents the fault status properly and is useful for accurate remaining life prediction. However, the conventional features have shown some limitations that make them less useful since they fluctuate over time even after the signal de-noising or do not show a distinct pattern of degradation which lack the monotonic trend over the cycles. In this study, a new method for feature extraction is proposed based on the observation of relative frequency energy shifting over the cycles, which is then converted into the feature using the information entropy. In order to demonstrate the method, traditional and new features are generated and compared using the bearing data named FEMTO which was provided by the FEMTO-ST institute for IEEE 2012 PHM Data Challenge competition.

• Structural Monitoring and Maintenance
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• v.2 no.1
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• pp.77-93
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• 2015

The idea of using measured dynamic characteristics for damage detection is attractive because it allows for a global evaluation of the structural health and condition. However, vibration-based damage detection for complex structures such as long-span cable-supported bridges still remains a challenge. As a suspension or cable-stayed bridge involves in general thousands of structural components, the conventional damage detection methods based on model updating and/or parameter identification might result in ill-conditioning and non-uniqueness in the solution of inverse problems. Alternatively, methods that utilize, to the utmost extent, information from forward problems and avoid direct solution to inverse problems would be more suitable for vibration-based damage detection of long-span cable-supported bridges. The auto-associative neural network (ANN) technique and the probabilistic neural network (PNN) technique, that both eschew inverse problems, have been proposed for identifying and locating damage in suspension and cable-stayed bridges. Without the help of a structural model, ANNs with appropriate configuration can be trained using only the measured modal frequencies from healthy structure under varying environmental conditions, and a new set of modal frequency data acquired from an unknown state of the structure is then fed into the trained ANNs for damage presence identification. With the help of a structural model, PNNs can be configured using the relative changes of modal frequencies before and after damage by assuming damage at different locations, and then the measured modal frequencies from the structure can be presented to locate the damage. However, such formulated ANNs and PNNs may still be incompetent to identify damage occurring at the deck members of a cable-supported bridge because of very low modal sensitivity to the damage. The present study endeavors to enhance the damage identification capability of ANNs and PNNs when being applied for identification of damage incurred at deck members. Effort is first made to construct combined modal parameters which are synthesized from measured modal frequencies and modal shape components to train ANNs for damage alarming. With the purpose of improving identification accuracy, effort is then made to configure PNNs for damage localization by adapting the smoothing parameter in the Bayesian classifier to different values for different pattern classes. The performance of the ANNs with their input being modal frequencies and the combined modal parameters respectively and the PNNs with constant and adaptive smoothing parameters respectively is evaluated through simulation studies of identifying damage inflicted on different deck members of the double-deck suspension Tsing Ma Bridge.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.5
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• pp.660-665
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• 2008

The aim of this work was to investigate the microbiological and physicochemical properties of the rice sourdough for Jeungpyun prepared by mixed culture of Saccharomyces cerevisiae (S. cerevisiae) and Leuconostoc mesenteroides (L. mesenteroides) strains. The rice sourdough was fermented with S. cerevisiae and L. mesenteroides strains in rice dough for 24 hours at $30^{\circ}C$. Growth of L. mesenteroides strain was decreased after inoculation, however, it increased again after 18 hours of dough fermentation, and the growth of S. cerevisiae showed a typical growth pattern. Also, total aerobic microorganisms counts in rice sourdough were decreased due to the produced organic acids and ethanol during dough fermentation. These products led to a favorable fermentative quotient (FQ; molar ratio between lactic to acetic acid) value of $1.9{\sim}3.2$ and more stable fermentation for rice sourdough formation. The expansion ratio and viscosity were considerably increased by mixed cultivation of S. cerevisiae and L. mesenteroides strains. Addition of the brown rice at 10% (w/w) to dough preparation increased the relative expansion ratio to the highest value.