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Control Efficacy of Bacillus thuringiensis against Herpetogramma luctuosalis on 'Campbell Early' Organic Vineyard (비티제를 이용한 포도 '캠벨얼리' 유기과수원 포도들명나방 방제효과)

  • Song, Myung-Kyu;Park, Jae-Seong;Lee, Seok-Ho;Lee, Jae-Wung;Kim, Seung-Duck;Choi, Won-Ho;Kim, Kyl-Ha;Park, Jong-Ho
    • Korean Journal of Organic Agriculture
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    • v.24 no.4
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    • pp.797-808
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    • 2016
  • The experiment of grape leafroller (Herpetogramma luctuosalis) was carried out at Okcheon area from 2007 to 2009 and 2015. The grape leafroller had been occurred at a campbell early' organic vineyard in Okcheon. It's larva was rolling the leaf of grape and ate the leaf. So the leaf of grape decreased. In organic vineyards, adult grape leafroller' generation rate per year showed the first peak in mid-June, the second peak in early -August and the third in mid-September. The larva showed the first peak in early July and the second peak in late August-early September. The grape leaf roller had three generations per year. And it took $60.9{\pm}1.09days$ from egg to adult in growth chamber (VS-91G09M-1300) which the relative humidity conditions was $60{\pm}10%$, temperature $25{\pm}2^{\circ}C$ and photoperiod 16L:8D (The egg : $12{\pm}0days$, larvae : $22.2{\pm}0.22days$, pupa : $10.6{\pm}0.75days$ and adult : $16.1{\pm}0.45days$). It was conducted to find out the effect of microbial pesticide treatments to control H. luctuosalis. The 4 microbial pesticides (Bacillus thurigiensis) were treated twice on the grape leaves in June 11 and 21 at an organic vineyard in Okcheon. On 10 days after last treatment, the control value of all microbial pesticides were more than 95%. When the dates of spraying to the grape leaves were on May 22, June 12 and July 2 each, the effects of microbial pesticide were 73.9%, 93.5% and 43.6% respectively. As a result, it was effective that Bt was sprayed to grape leaves on mid and late June for controling the H. luctuosalis in organic vineyard. And microbial pesticide Bt was thought to be useful to control the grape leafroller in organic vineyard.

Development of an Efficient Screening System for Resistance of Watermelon Plants to Didymella bryoniae (수박 덩굴마름병에 대한 효율적인 저항성 검정 방법 개발)

  • Lee, Ji Hyun;Jang, Kyoung Soo;Choi, Yong Ho;Kim, Jin-Cheol;Choi, Gyung Ja
    • Research in Plant Disease
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    • v.22 no.2
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    • pp.72-80
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    • 2016
  • Gummy stem blight, caused by the fungus Didymella bryoniae, is major disease of watermelons worldwide. The objective of the present study was to establish an efficient screening system to identify watermelon resistant to D. bryoniae. An GSB3 isolate was prepared from a watermelon plant showing typical symptoms of gummy stem blight in Haman-gun and identified as D. bryoniae based on molecular analysis of internal transcribed spacer sequence. A simple mass-production technique of inoculum was developed based on spore production of D. bryoniae GSB3 under several incubation conditions and their virulence on watermelon plants. Resistance degrees of 22 commercial watermelon cultivars to the GSB3 isolate were evaluated. Among them, four watermelon cultivars showing different degree of resistance response were selected for further study. Development of disease on the cultivars according to various conditions including inoculum concentrations, incubation periods in dew chamber, and incubation temperatures was investigated. From the results, we suggest an efficient screening method for resistant watermelon cultivars to gummy stem blight. Seeds of watermelon cultivar are sown and grown in a greenhouse until plant stage of 2-fully expanded leaves. Seedlings are inoculated with D. bryoniae by spraying spore suspension of the fungus at a concentration of $5.0{\times}10^5spores/ml$. The infected plants are incubated in humidity chamber at $25^{\circ}C$ for 48 hours and then transferred to a growth chamber at $25^{\circ}C$ and 80% relative humidity with 12-hour light a day. Three to four days after inoculation, disease severity of the plant are measured using percentage of infected leaf area.

Effect of ${\beta}$-glucan on Growth, Feed Efficiency and Hematologic Index in Sparague-Dawley Rats (${\beta}$-glucan이 Sparague-Dawley 랫드의 성장, 식이효율 및 혈액성상에 미치는 효과)

  • kim, So-Jung;Lee, Jin-Seok;Kwon, Jung-Ki;An, In-Jung;Lee, Seung-Ho;Park, Young-Seok;Park, Byung-Kwon;Kim, Byeong-Soo;Kim, Sang-Ki;Song, Sung-Ki;Lee, Jong-Dae;Cho, Sung-Doe;Choi, Chang-Sun;Jung, Ji-Youn
    • Journal of Food Hygiene and Safety
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    • v.26 no.1
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    • pp.49-56
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    • 2011
  • To investigate the toxicological effects of ${\beta}$-glucan, we performed basic studying on ${\beta}$-glucan in Sprague-Dawley (SD) rats. Standard endpoints in this study included mortality, clinical observations, changes of body weights, analysis on food consumption, ophthalmoscopic examination, hematologic examination, serum biochemistry, analysis of organ weights, gross anatomic pathology and histopathology. No clinical signs and mortality were observed in animals treated with beta-glucan throughout the experimental period. The average body weight of each treatment groups showed similar levels at end of experiment. There were no treatment-related changes in mortality, body weights changes, food consumption, ophthalmoscopic examination. Although there were statistically significant differences between the control and treated groups in some relative and absolute organ weights, and hematological and biochemical analysis, the data were in biologically normal ranges and did not show a dose-dependent manner. In the morphological change, hepatic tissue were not showed ballooning degeneration and irregular arrangement of hepatic cell in ${\beta}$-glucan treatment groups with control group. Also, organs weights (liver, heart, kidney and stomach) and hematological indices (WBC, RBC, Hb, Hct and Platelet) did not show statistically significant differences among the experimental groups. In summary of these results, there were no changes in mortality, mean body weight, clinical signs, food consumption. There were no changes in ophthalmological examination, hematology, blood chemistry, necropsy and histopathology. In conclusion, although further investigation of glucan should be performed in the functions registered in many ancient literatures, ${\beta}$-glucan is physiologically safe and may have potential as candidate food for human health.

Birth and Transformation of the Concept of "Oriental-ness" in Korean Art (한국미술에서의 동양성 개념의 출현과 변형)

  • Chung, Hyung-Min
    • The Journal of Art Theory & Practice
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    • no.1
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    • pp.109-144
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    • 2003
  • Orientalness is a concept that expresses the collective identity of the Orient in relation to the West. The concept itself is mutable and defined by the relationship between the two regions at different points in time. Changes in the concept depend on a number of factors, such as cultural influence, the political balance of power between the two regions, and on the interpretative scheme that defines the relationship. In addition, the geographical notion of the concept evolves culturally, socially and politically. During this process, Oriental-ness becomes Oriental-ism at times. I will attempt to survey and measure the progression of Orientalness from its emergence in early 17th century to its subsequent transformation in modern Korea as reflected in art theory and art works. The recognition of the comparative characteristics of Oriental art began when the Orient was exposed to the art of the West in the late Ming dynasty during the early 17th century. The changes in the artistic climate in China affected the late Chosun. I will start with a brief introduction of this time and the birth of Orientalness. The concept gradually changed during the period of Enlightenment(開化期) towards the end of the 19th century, and during the colonial period( 1910-1945) it took on a new form. Establishment of the concept of "Orient"as a single, unifying concept spanning across cultures and national boundaries has been attributed to late Meiji period Japan, whose intention at that time is believed to have been to build a pan-Asia(亞細亞) empire with Japan at its commanding center. It has been stressed that the real motive behind the formation of one single cultural unit, where the shared common written language was Chinese and Confucianism and Taoism were the common metaphysical traditions, was to build one political unit. When the notion of a geographical unit of Asia was replaced by the concept of Asia as a cultural and political unit, a massive growth of interest and discourse were provoked around the concept of Orientalism. When Orientalism was being formulated, Korea automatically became member of "one Asia" when the country became colonized. For Koreans, the identity of the Orient had to be defined in cultural terms, as the political notion of a nation was non-existent at that time. The definition of identity was pursued at two levels, pan-Asian and local. If Orientalism was an elite discourse centered in pan-Asian philosophical and religious tradition, localized Orientalism was a popular discourse emphasizing locality as the byproduct of natural geographic condition. After the liberation in 1945 from colonial rule, a thrust of movement arose towards political nationalism. Two types of discourses on Orientalism, elite and popular, continued as central themes in art. Despite the effort to redefine the national identity by eradicating the cultural language of the colonial past, the past was enduring well into the present time. As discussed above, even when the painting themes were selected from Korean history, the tradition of using history painting as a manifestation of political policy to glorify the local identity had its founding during the Meiji period. The elevation of folk art to the level of high art also goes back to the colonial promotion of local color and local sentiment. Again, the succession of the past (colonial) ideal was defended as the tradition assumed a distinct modern shape that was abstract in style. The concept of the "Orient" is of relative and changing nature. It was formulated in relation to Western culture or civilization. Whatever the real motive of the adoption of them had been, the superiority of the Orient was emphasized at all times. The essence of the Orient was always perceived as the metaphysical tradition as a way to downgrade Western culture as materialistic. This view still prevails and the principle of Orient was always sought in Confucianism, Taoism, and Buddhism. Even when Orientalism was employed by imperialist Japan in an effort to establish her position as the center of the Orient, the spiritual source was still in Chinese philosophy and religion. In art also, the Chinese literati tradition became the major platform for elite discourse. Orientalism was also defined locally, and the so-called local color was pursued in terms of theme and style. Thus trend continued despite the effort to eradicate the remnants of colonial culture long after liberation. These efforts are now being supported politically and also institutionalized to become the aesthetic ideal of the modern Korean art.

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Effect of substrate bias voltage on a-C:H film (기판 bias 전압이 a-C:H 박막의 특성에 미치는 영향)

  • 유영조;김효근;장홍규;오재석;김근식
    • Journal of the Korean Vacuum Society
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    • v.6 no.4
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    • pp.348-353
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    • 1997
  • Hydrogenated amorphous carbon(a-C:H) films were deposited on p-type Si(100) by DC saddle-field plasma enhanced CVD to investigate the effect of substrate bias on optical properties and structural changes. They were deposited using pure methane gas at a wide range of substrate bias at room temperature and 90 mtorr. The substrate bias voltage ($V_s$) was employed from $V_s=0 V$ to $V_s=400 V$. The information of optical properties was investigated by photoluminescence and transmitance. Chemical bondings of a-C:H have been explored from FT-IR and Raman spectroscopy. The thickness and relative hydrogen content of the films were measured by Rutherford backscattering spectroscopy (RBS) and elastic recoil detection (ERD) technigue. The growth rate of a-C:H film was decreased with the increase of $V_s$, but the hydrogen content of the film was increased with the increase of $V_s$. The a-C:H films deposited at the lowest $V_s$ contain the smallest amount of hydrogen with most of C-H bonds in the of $CH_2$ configuration, whereas the films produced at higher $V_s$ reveal dominant the $CH_3$ bonding structure. The emission of white photoluminescence from the films were observed even with naked eyes at room temperature and the PL intensity of the film has the maximum value at $V_s$=200 V. With $V_s$ lower than 200 V, the PL intensity of the film increased with V, but for V, higher than 200 V, the PL intensity decreased with the increase of $V_s$. The peak energy of the PL spectra slightly shifted to the higher energy with the increase of $V_s$. The optical bandgap of the film, determined by optical transmittance, was increased from 1.5 eV at $V_s$=0V to 2.3 eV at $V_s$=400 V. But there were no obvious relations between the PL peak and the optical gap which were measured by Tauc process.

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Water Environment and Freshwater Algae in the Upstream of the Tamjin River Dam (탐진강댐 상류하천에서 수환경과 담수조류)

  • Shin, Jae-Ki;Cho, Kyung-Je
    • Journal of Environmental Impact Assessment
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    • v.10 no.2
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    • pp.109-121
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    • 2001
  • Water environment and freshwater algae were studied in the upstream of the Tamjin River Dam. Among the environmental factors, DO concentration in the Tamjin River ranged from 9.0 mg $O_2/l$ to 9.2 mg $O_2/l$, pH from 7.0 to 7.1 and conductivity from $98{\mu}S/cm$ to $100{\mu}S/cm$. Average concentration of $NH_4$ and $NO_3$ ranged from $40{\mu}g\;N/l$ to $56{\mu}g\;N/l$ and from $489{\mu}g\;N/l$ to $611{\mu}g\;N/l$, respectively. $NO_3$ was more plentiful above 9~15 fold than that of $NH_4$. Average concentrations of soluble reactive phosphorus and soluble reactive silicon were $2{\mu}g\;P/l$ and 1.6 mg Si/l, respectively. Particulaly, Si nutrient increased by heavy rain events during summer season. The ratios of N/P and Si/P ranged from 248 to 261 and from 640 to 740, respectively. It is assumed that P would be limiting nutrient on the freshwater algal growth. Average content of planktonic chlorophyll-a ranged from $5{\mu}g/l$ to $13{\mu}g/l$. Mean contents of chlorophyll-a, phaeo-pigment and ash-free dry matter of periphyton were $50.3mg/m^2$, $11.9mg/m^2$, $11.5g/m^2$ in the main stream and $30.1mg/m^2$, $5.6mg/m^2$, $7.8g/m^2$ in the tributary. By comparison of the epilithon biomass, the main stream was higher with 1.5~2.1 fold than the tributary. The impotant algae were composed of diatom Achnanthes linearis, A. minutissima, Fragilaria crotonensis, Gomphonema gracile, Tabellaria flocculosa and blue-green algae Microcystis aeruginosa. In the relative abundance of the phytoplankton and epilithon, the serial dominance were diatom > green algae > blue-green algae, and diatoms were very abundant in comparison with other algal phylum.

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Study on the Food Habits of Sika Deer(Servus Nippon) Fed with Various Roughage Sources (여러 가지 조사료를 급여한 꽃사슴(Servus nippon)의 채식습성에 관한 연구)

  • Lee, In-Duk;Lee, Hyung-Suk
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.28 no.1
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    • pp.61-70
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    • 2008
  • The object of this experiment was to investigate the food habits of sika deer fed with various roughage sources. The feeding trials were conducted at Chungnam National University and Unbong Animal Genetic Resources Station in 2007. The experimental roughages sources were five including 25 species in all; grass sources; 5 species(Lolium perenne, Festuca arundinacea, Poa pratensis, Agrostis alba and mixed grass), legume sources; 5 species(Trifolium pratense, Medicago sativa, Trifolium repens, Melilotus officinalis and Vicia villosa), browse sources; 5 species(Quercus serrata Thunb., Prunus jamasakura Sieb., Quercus aliena Blume, Robinia pseudoaccacia and Pinus densiflora), weed sources; 5 species(Artemisia princeps Pampanini, Erigeron canadensis, Alopecurus aegualis Sobolewski, Echinochloa crusgalli var. frumentacea(Roxb.) Wight and Rumex crispus), and native grass sources; 5 species(Zoysia japonica Steudel, Agropyron tsukushiense var. transiens(Hack) Ohwi., Arundinella hirta(Thunb.) Tanaka, Miscanthus sinensis Anderss and Phragmites comunis Trin). Five sika deer were selected which had nearly the same body weight(average 92.5kg) as experimental animal. Experimental roughages were prepared by 0.5kg(DM basis) per 1 species fed to sika deer, and the experiment was carried out from 08:00 to the next day 06:00. The chemical composition and dry matter digestibility of each roughage source and species were significantly different at the sampling area, plant species, growth stages and cutting period. The sika deer ate more roughages which had low fibrous contents, but high dry matter digestibility. Among all the 25 species of roughages, the favorite intake species ranking by sika deer was observed like this: Quercus aliena Blume, Prunus jamasakura Sieb, Quercus serrata Thunb. and Trifolium pratense, respectively, and the lower intake species was Phragmites comunis Trin. On the other hand, compared to each roughage source, total intake amount by sika deer was showed as browse(36.5%), Legumes(34.2%), grasses(14.0%), native grasses(13.5%) and weeds(1.8%), respectively. Based on the result, the food habits of sika deer seems to be closer to typically browser type.

Chromosomal analyses of 4,500 cases of the peripheral blood : An experience in a single hospital for 25 years (말초혈액을 이용한 핵형 분석 4,500례 : 단일기관에서의 25년간의 경험)

  • Seo, Hye-Eun;Lee, Ji Hye;Kim, Ji Yoon;Lee, Dong Ha;Lee, Heung Kyo;Lee, Kun Soo
    • Clinical and Experimental Pediatrics
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    • v.50 no.9
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    • pp.875-881
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    • 2007
  • Purpose : Chromosome analysis is important in genetic study and genetic counseling. This study was performed to evaluate the type and incidence of chromosome abnormalities in a single hospital for 25 years. Methods : Chromosome analyses were performed on peripheral blood lymphocytes, obtained from 4,856 patients with suspected chromosomal aberrations, referred to cytogenetic laboratory in Department of Pediatrics, Kyungpook National University Hospital from May 1981 to October 2005. Results : We analyzed 4,567 cases. Children were 3,014 cases (66.0%) and adult were 1,553 cases (34.0 %). The most common purpose of the chromosomal analysis was growth and developmental abnormality in children and infertility in adults. Total chromosomal aberration rate was 16.9% (770/4,567). Among those cases, the numerical abnormalities were 12.2% (558 cases), the structural abnormalities were 4.1% (187 cases), and others were 0.5% (25 cases). The relative frequencies of autosomal abnormalities were 6.4% (294 cases) in Down syndrome; 0.2% (7 cases) in Edwards syndrome; 0.1% (4 cases) in Patau syndrome; 0.2% (10 cases) in other abnormalities, of sex chromosome, 2.9% (131 cases) in Klinefelter syndrome; 2.2% (99 cases) in Turner syndrome; 0.2% (8 cases) in 47, XXX; 0.1% (3 cases) in 47, XYY. Among the structural abnormalities, translocation was 1.8% (84 cases), inversion was 0.8% (37 cases), deletion was 0.4% (17 cases), and insertion was 0.3% (13 cases), in order of frequency. Conclusion : In this study, the type, incidence and distribution of cytogenetic abnormalities by karyotype were reviewed. We hope that our study could be used as a basic information on the diagnosis, treatment and genetic counseling for chromosome abnormalities in Korea.

Analytical method of aflatoxins in edible oil and infant-children foods (식용유지와 영유아식품 중 아플라톡신 분석방법)

  • Hu, Soo-Jung;Park, Seung-Young;Kim, Soon-Sun;Lee, Joon-Goo;Song, Ji-Young;Kang, Eun-Gi;Lee, Hyun-Sook;Cho, Dae-Hyun
    • Analytical Science and Technology
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    • v.24 no.2
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    • pp.150-157
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    • 2011
  • Aflatoxins are secondary metabolites of the molds of Aspergillus flavus and Aspergillus parasiticus. They are highly carcinogenic compounds and can affect a wide range of vegetable commodities such as cereals (especially corn), nuts, peanuts, fruits and oil seeds, in the field and during storage. In fact, oilseeds are often stored for weeks in conditions that promote the mould growth, and the possible consequent presence of aflatoxins in oilseeds can lead to their transfer in oil. In addition, aflatoxins can be found as a natural contaminant in multi-cereals and beans making baby food for infants and young-children. The objective of this study was to validate the liquid extraction method or develop an analytical method for edible oil and infant-children foods. Therefore, this study developed condition of extract for aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) in edible oil using a high performance liquid chromatography with florescence detector (HPLC/FLD). Aflatoxins were extracted from edible oil samples by means of MSPD (Matrix solid phased dispersion), utilizing $C_{18}$ as dispersing material and purified by using immunoaffinity column. The gression line coefficients were above 0.999. The recoveries for aflatoxins ranged from 85.9 to 93.0%, and relative standard deviations were below 5.7%. The new developed method of aflatoxins effectively enhanced recoveries by using MSPD-Immunoaffinity column compared with liquid extraction. The analytical method for liquid extraction of aflatoxin was appropriate for infant-children food. Reviewing the current method, the recoveries of aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) were 89.5~92.3%.

Analysis on Estimating Evapotranspiration of Paddy Rice (벼의 증발산량(蒸發散量) 산정(算定)에 관(關)한 실험(實驗) 분석(分析))

  • Suh, Seung Duk;Lee, Jong Kook
    • Current Research on Agriculture and Life Sciences
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    • v.3
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    • pp.28-35
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    • 1985
  • This work Was carried out to obtain the most suitable crop coefficient for the paddy rice growing in Taegu area. The result was due to the comparative measurements of evapotranspiration formula in terms of Blaney & Criddle and eight other formulas with those produced by experiment particularly in this area. The crop coefficient, evapotranspiration and transpiration ratio produced by this research are hopefully expected to be of service in the future calculation of evapotranspiration without repeating experiment respectively, whenever the water requirement of paddy rice is planned in Taegu and its vicinity. The accomplished results could be summarized as follows : The maximum amount of evapotranspiration was recorded in the early and middle parts of August. The average reading of evapotranspiration was 6.33mm/day throughout the growth. The evapotranspiration had a highly significant correlation with pan evaporation, solar radiation, sunshine hours and relative humidity of meteorological elements. K and Kc by the use of Blaney & Criddle formula calculated at 0.76 to 1.45 and 0.82 to 1.27, respectively. Its peak value appeared commonly in early August. The ratio of transpiration was 269.03.

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