Analytical Science and Technology (분석과학)

The Korean Society of Analytical Sciences (한국분석과학회)
권호 표지
DOI QR코드

DOI QR Code

Analytical method of aflatoxins in edible oil and infant-children foods

식용유지와 영유아식품 중 아플라톡신 분석방법

  • Hu, Soo-Jung (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Park, Seung-Young (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Kim, Soon-Sun (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Lee, Joon-Goo (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Song, Ji-Young (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Kang, Eun-Gi (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Lee, Hyun-Sook (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration) ;
  • Cho, Dae-Hyun (Hazardous Substances Analysis Division, Incheon Regional Food & Drug Administration)
  • 허수정 (경인지방식품의약품안전청 유해물질분석과) ;
  • 박승영 (경인지방식품의약품안전청 유해물질분석과) ;
  • 김순선 (경인지방식품의약품안전청 유해물질분석과) ;
  • 이준구 (경인지방식품의약품안전청 유해물질분석과) ;
  • 송지영 (경인지방식품의약품안전청 유해물질분석과) ;
  • 강은귀 (경인지방식품의약품안전청 유해물질분석과) ;
  • 이현숙 (경인지방식품의약품안전청 유해물질분석과) ;
  • 조대현 (경인지방식품의약품안전청 유해물질분석과)
  • Received : 2011.02.25
  • Accepted : 2011.03.21
  • Published : 2011.04.25
Download PDF
⟨ Previous Next ⟩

Abstract

Aflatoxins are secondary metabolites of the molds of Aspergillus flavus and Aspergillus parasiticus. They are highly carcinogenic compounds and can affect a wide range of vegetable commodities such as cereals (especially corn), nuts, peanuts, fruits and oil seeds, in the field and during storage. In fact, oilseeds are often stored for weeks in conditions that promote the mould growth, and the possible consequent presence of aflatoxins in oilseeds can lead to their transfer in oil. In addition, aflatoxins can be found as a natural contaminant in multi-cereals and beans making baby food for infants and young-children. The objective of this study was to validate the liquid extraction method or develop an analytical method for edible oil and infant-children foods. Therefore, this study developed condition of extract for aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) in edible oil using a high performance liquid chromatography with florescence detector (HPLC/FLD). Aflatoxins were extracted from edible oil samples by means of MSPD (Matrix solid phased dispersion), utilizing $C_{18}$ as dispersing material and purified by using immunoaffinity column. The gression line coefficients were above 0.999. The recoveries for aflatoxins ranged from 85.9 to 93.0%, and relative standard deviations were below 5.7%. The new developed method of aflatoxins effectively enhanced recoveries by using MSPD-Immunoaffinity column compared with liquid extraction. The analytical method for liquid extraction of aflatoxin was appropriate for infant-children food. Reviewing the current method, the recoveries of aflatoxins ($B_1$, $B_2$, $G_1$ and $G_2$) were 89.5~92.3%.

아플라톡신은 Aspergillus flavus와 A. parasiticus에 의해 생성되는 독소대사산물로 발암물질이며 곡류(옥수수, 쌀, 보리), 땅콩, 과실류 및 종자류의 생산과 저장과정에서 생성된다. 곰팡이가 생성되기 쉬운 조건에서 오랜 기간 저장된 종자류에서 아플라톡신이 생성될 가능성이 있으며 이를 원료로 하여 유지로 가공 할 때도 아플라톡신이 이행될 우려가 있다. 또한 곡류나 두류 등을 가공하여 만든 영유아용식품도 아플라톡신 오염 가능성이 있다. 따라서 본 연구는 식용유지 및 영유아용식품에 대해 액체 추출법의 아플라톡신 시험법 적용가능 여부를 검토하고 필요시 새로운 분석방법을 확립하고자 하였다. 식용유지에 대한 아플라톡신은 MSPD (Matrix Solid Phase Dispersion)법으로 아플라톡신을 추출해 내고 면역친화성 칼럼을 사용해 정제하여 형광검출기가 장착된 고성능액체크로마토그래피(HPLC/FLD)를 이용해 분석하였다. 아플라톡신($B_1$, $B_2$, $G_1$, $G_2$) 검량선의 직선성은 상관계수가 0.999 이상을 나타냈고 회수율은 85.9~93.0%의 양호한 결과를 얻었으며 상대표준편차는 5.7% 이하였다. 식용유지에서 액체 추출법과 비교해 볼 때, MSPD-면역친화성컬럼법을 사용하여 회수율을 향상시켜 시험법을 확립하였다. 영유아용식품에 대한 아플라톡신 분석방법은 액체 추출법이 적합하였으며 아플라톡신($B_1$, $B_2$, $G_1$, $G_2$)에 대한 회수율은 89.5~92.3%로 양호한 결과를 얻었다.

Keywords

References

  1. IARC, Internation Agency for Reserch on Cancer, Lyon, Mycotoxins, 169-366(2002).
  2. M. Martins, H. I. Tins and F. Bernardo, Food Additives and Food Science, 1, 63-67(2001).
  3. S. V. Reddy, K. D. Mayi, R. M. Uma, K. Thrumala- Devi and D. V. Reedy, Food Additives and Contamination, 18, 55-558(2001).
  4. T. Asao, G. Buchi, M. M. Abdel-Kader, S. B. Chang, E. L. Wick and G. N. Wagan, J. Am. Chem. Soc. 85, 1760-1770(1963).
  5. H. S. Hussein and M. B. Jeffery, Toxicology, 167, 101-134(2001). https://doi.org/10.1016/S0300-483X(01)00471-1
  6. K. C. Sargeant, Chemical Ind. London, 53-55(1963).
  7. Handbook of toxic fungal metabolites. Academic Press, New York, 1-66(1981).
  8. M. Holcomb, D. M. Wilson, M. W. Truckness and H. C. Thomason, J. Chromatography, 624, 341-352(1992). https://doi.org/10.1016/0021-9673(92)85687-O
  9. J. K Kim, J. Food Hyg. Safety, 13, 41-46(1998).
  10. 황준호, 전향숙, 이광근, J. Korean Soc. Appl. Biol. Chem. 47(1), 1-16(2004).
  11. M. P. Elizalde-Gonzalez, J. Mattusch and R. Wennrich, J. Chromatography. 828, 439-444(1998). https://doi.org/10.1016/S0021-9673(98)00702-X
  12. P. B. Andri, S. Joerg and A. Elke, J. AOAC International, 85(2), (2002).
  13. A. Carisano and G. D. Torre, J. Chromatography A, 355, 340-344(1986). https://doi.org/10.1016/S0021-9673(01)97334-0
  14. A. E. Waltking and D. Wilson, J. AOAC International, 89(3), 678-692(2006).
  15. D. M. Takahashi, J. Chromatography A, 131, 147-156 (1977). https://doi.org/10.1016/S0021-9673(00)80928-0
  16. 식품공전 (2010).
  17. C. Cavaliere, P. Foglia. C. Guarino, M. Nazzari, R. Samperi and A. Lagana, Analytica Chimica Acta, 596, 141-148(2007). https://doi.org/10.1016/j.aca.2007.05.055
  18. J. Stroka and E. Anklam, J. AOAC International, 84, 4 (2001).
  19. J. Tam, M. Mankotia, M. Mably, P. Pantazopoulous, R. J. Neil, P. Calway and P. M. Scott, Food Additives and Contamination, 23(7) 639-699(2006).
  20. P. C. Alvito, E. A. Sizoo, C. M. Almeida and H. P. Egmond, Food Anal. Methods, 3, 22-30(2010). https://doi.org/10.1007/s12161-008-9064-x