• Journal of Life Science
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• v.25 no.1
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• pp.75-83
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• 2015

Curcuma longa L. (CL), a traditional medicinal plant, is well known as a functional food ingredient. The major component of CL is a curcumin of anthocyanin family that has multi-functions such as antimicrobial, anticancer, and antioxidant activity. In this study, fermented milk containing CL was prepared using a mixed strain culture (Bifidobacterium bifidus, Streptococcus thermophilus, Lactobacillus acidophilus), and its physicochemical properties were characterized. In addition, inflammatory cytokine-modulating effects of the fermented milk were also investigated. As regards the properties of fermented milk, the growth rate of lactic acid bacteria in fermented milk containing CL was found to be remarkably more rapid than control. During fermentation, caseins and whey proteins were observed to be partially hydrolyzed, and lactic acid and acetic acid were produced in larger amounts than in the control. The sensory score of fermented milk containing CL was lower than control, owing to its bitter taste and strong flavor. RAW 264.7 cells treated with CL fermented milk supernatant showed no cytotoxicity. Inflammatory cytokines such as tumor necrosis factor-alpha (TNF-${\alpha}$) and interleukin-6 (IL-6) were significantly produced by fermented milk with CL, compared to control. The secretion of nitric oxide (NO) from RAW 264.7 cells significantly increased relative to the control. Results from the present study suggested that CL could be used as a natural immunomodulating ingredient for making yogurts, beverages, and other products.

• Journal of Life Science
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• v.19 no.1
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• pp.1-8
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• 2009

In this paper, to elucidate the further mechanisms of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG)-induced growth arrest, we investigated the effect of MNNG on cell cycle and proliferation in U937 cells, a p53-null human myeloid leukemia cell line. It was found that MNNG causes an arrest at the G2/M phase of the cell cycle and induces apoptosis, which is closely correlated to inhibition of cyclin B1 and cyelin-dependent kinase (Cdk) 2-associated kinase activities. MNNG treatment in. creased protein and mRNA levels of the Cdk inhibitor p21(WAF1/CIP1), and activated the reporter construct of a p21 promoter. By using p21 promoter deletion constructs, the MNNG-responsive element was mapped to a region between 113 and 61 relative to the transcription start site. These data indicate that in U937 cells MNNG can circumvent the loss of wild-type p53 function and induce critical downstream regulatory events leading to transcriptional activation of p21. Present results indicate that the p53-independent up-regulation of p21 by MNNG is likely responsible for the inhibition of cyclin/Cdk complex kinase activity rather than the down-regulation of cyclins and Cdks expression. These novel phenomena have not been previously described and provide important new insights into the possible biological effects of MNNG.

• Journal of Life Science
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• v.24 no.12
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• pp.1330-1338
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• 2014

In Mycobacterium tuberculosis H37Rv 16 adenylyl cyclase (AC) genes have been identified, while 10 AC genes have been found in non-pathogenic Mycobacterium smegmatis. Expression of 6 AC genes (MSMEG_0218, MSMEG_3243, MSMEG_3780, MSMEG_4279, MSMEG_4477, and MSMEG_6154) among 10 AC genes in M. smegmatis was increased when M. smegmatis was subjected to hypoxic growth conditions. On the other hand, only MSMEG_3780 and MSMEG_4279 were slightly induced in the presence of NO. The cAMP levels in cells and culture media were 450- and 9.8-fold increased, respectively, when M. smegmatis was grown under hypoxic conditions relative to those grown aerobically. Intracellular levels of cAMP were increased 5.8-fold on the exposure to NO. The DevSR two-component system is known to be involved in the induction of many genes whose expression is induced under hypoxic conditions and in the presence of NO. Expression of 6 hypoxically induced AC genes was observed to be induced in a devR deletion mutant grown under hypoxic conditions, indicating that the induction of the 6 AC genes under hypoxic conditions is independent of the DevSR two-component system. In order to identify a trans-acting regulatory element that is pertinent in the hypoxic induction of MSMEG_3780, ligand-fishing analysis was performed using the upstream DNA of MSMEG_3780 and MSMEG_5136 protein was identified.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.2
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• pp.146-154
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• 2014

In the result of effect on freezing damage reduction by the shade net colors and the shade rate to tea trees during wintering period, the high shade ratio decreased tea trees growth and increased freezing damage and 55% of shade ratio based on non treatment developed new leafs and green leaf productivity. By the shade net colors, colorless shade net (55% of shade type) treatment and green shade net treatment increased green leaf productivity and decreased damaged area compare to non treatment shade net and black shade net treatment. Colorless net shade treatment reduced over 50% of freezing damage and increased (10a) 68 kg for green leaf productivity compare to non treatment shade net. The colors of shade net treatments for reduction freezing damaged in order of Black < Green < Colorless but reduction of freezing damage was so high. In the relative microclimates, treatment shade nets were $0.7^{\circ}C$ lower, average relatively humidity 14.9% higher, soil temperature $0.6^{\circ}C$ lower and soil moisture 4.6% higher than non treatment shade net. And treatment shade nets decreased average wind speed 0.7 m/s and it showed us treatment net shades effected to excessive evapotranspiration and soil dry by wind and considered one of good solution for freezing damages.

• Journal of Bio-Environment Control
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• v.9 no.3
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• pp.156-160
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• 2000

The Plasma film treated with a high electric voltage was developed to enhance flow down of condensation drops on inside plastic film. Arch type greenhouse framed with iron pipe of 25mm diameter defand 1.5mm thickness were covered with either the developed plasma film or surfactant film(control). Green pepper seedlings raised for 40 days in plug trays were transplanted at a density of 110cm by 30cm in each greenhouse. The mount of condensational water on film surface, generated by 7$0^{\circ}C$ water bath chimney systems and flew down, was collected and measured. The amount of collected water after 150 minutes was 2.56 mL.100c $m^{-2}$ and 0.94mL.100c $m^{-2}$ , respectively, in the plasma film and surfactant film-covered greenhouses. The amount of condensational water drops attached on the cover at 08:20 a.m. at 60 days filter covering was 0.34mL.100c $m^{02}$ and 0.32mL.100c $m^{-2}$ , respectively, in the plasma film- and surfactant film-covered greenhouses. Solar irradiance transmitted into greenhouse was 2.0% higher in the greenhouse covered with the plasma film tan that in the greenhouse covered with the surfactant film. Air temperature in the plasma film-covered greenhouse was higher than the surfactant film-covered greenhouse by 0.5$^{\circ}C$. However, there was no difference in relative humidity between the two greenhouse. Plant height, leaf area, dry weight and early yield showed no significant differences.s.

• Applied Microscopy
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• v.42 no.1
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• pp.17-26
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• 2012

Histologic and microstructural changes during the postembryonic development of the wolf spider Arctosa kwangreungensis were studied using light and scanning electron microscopy to examine the relationship between a morphological differentiation and behavioral properties. The postembryo with abdominal yolk sac was stayed inactive in the egg case because its muscular and visual systems were not fully developed to a functional level. The first instar spiderlings, developed from the postembryo by a first molting process, started to exhibit its pigmentation on their body cuticles. In particular, undifferentiated cell clusters of central nervous system (CNS) were densely distributed within the cephalothorax, and highly differentiated abdominal ganglion was observed. They had a characteristic visual system looks more like its adult counterpart, and had segmented appendages looks more like the tiny spiders containing well oriented muscular system. After 3rd instar, spiderlings grew more rapidly with accordance to their consistent growth and periodical molting processes. Thus, the relative area of CNS with respect to cephalothorax was gradually decreased, instead a pair of venom glands, musculature, and connectives occupied the residual area. It has been revealed that the early development of spider can be controled by the feeding condition of larval period, since histologic and microstructural differentiations in both appendages and optic system were completed at the second instar. In particular, behavioral properties of the wandering spiders that depend on vision and their running ability were deeply related to physiological differentiation of the microstructural development.

• Korean Journal of Agricultural and Forest Meteorology
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• v.5 no.2
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• pp.51-60
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• 2003

Rice, which occupies about 60% of the farmland in Korea, is a staple crop in Asia. It not only absorbs $CO_2$ from the atmosphere, but also emits carbon in a form of CH$_4$. It has a potential role in the global budget of greenhouse gases because of its relative contributions of carbon absorption and emission associated with changing hydrologic cycle. To better understand its current and future role, seasonal variations of energy and $CO_2$ exchange in this critical ecosystem need to be quantified. The purpose of this study was to measure, document and understand the exchange of energy and $CO_2$ in a typical rice paddy in Korea throughout the whole growing season. Since late April of 2002, we have conducted measurements of energy and $CO_2$ exchange in a rural rice paddy at Hari site, one of the Korea regional network of tower flux measurement (KoFlux). After the quality control and gap-filling, the observed fluxes were analyzed in the context of micrometeorology and biophysics. $CO_2$ and energy exchanges varied significantly with land cover changes (e.g., plant growth stages), in addition to changes in weather and climate conditions. This study, reporting first direct measurement of energy and $CO_2$ exchange over a rice paddy in Korea, would serve as a useful database as one of the reference sites in AsiaFlux and FLUXNET.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.8
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• pp.1197-1204
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• 2018

Objective: The objective was to characterize the fermentative and microbiological profile of Tifton 85 bermudagrass haylage with different layers of polyethylene film and storage time. Methods: The experimental design consisted of a randomized block design with four and six wrapping layers (100 and 150 microns in total. respectively) allocated in the main plots, through repeated measures analysis (30, 60, and 90 days of storage) with four replicates. Results: The storage time and number of wrapping layers did not show changes in the population of Clostridium and lactic acid bacteria. A decrease was observed in the enterobacteria population with an increase in the storage period in the two wrapping layers studied. Upon opening of the haylage at 30 days, the population of Bacillus was lower in haylages made with six layers of wrapping (3.63 log colony forming units/g). No growth of Listeria sp. or Salmonella sp. was observed during the experimental period. The fungal genera with a greater occurrence were Penicillium sp. and Fusarium sp. The following mycotoxins were not detected: ochratoxin A, fumonisins, and zearalenone. Relative to the organic butyric, propionic, and acetic acids, the haylages presented a low concentration of lactic acid; this may have prevented a drop in the pH, which was high when the silos were opened (5.4). The levels of ammoniacal nitrogen and soluble carbohydrates presented no variation among the number of wrapping layers, with an overall average of 35.55 and 38.04 g/kg. Conclusion: Tifton 85 bermudagrass haylage wrapped with four and six layers presented adequate fermentation and microbiological characteristics in the evaluated periods.

• Journal of Bio-Environment Control
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• v.29 no.3
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• pp.293-305
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• 2020

This experiment was aimed to identify concentrations of mineral nutrients in leaf lettuce (Lactuca sativa) grown on hydroball aquaponics and in the water for growing fish by conducting two experiments. The experiment I (Expt. I) was conducted with 12 fishes (F12) with and without filter, hydroball and plants (H12 (12 fishes, hydroball), FHP12 (12 fishes, filter, hydroball, 6 plants) and HP12 (12 fishes, hydroball, 6 plants)), and the experiment II (Expt. II) was with and without plants (FH15 (15 fishes, filter, hydroball), FHP15 (15 fishes, filter, hydroball, 6 plants)). The pH level in the water of all the treatments was decreased during the growing period, and the pH and EC of the water were lower in all the treatment with plants than those without plants in both Expt. I and Expt. II. When compared with adequate nutrient concentrations for hydroponics, nitrate nitrogen (NO₃-N) and phosphorus (P) concentrations in the fish growing water were higher under the FHP15 treatment in Expt. II; however, potassium (K), calcium (Ca), and magnesium (Mg) were only 16, 49, and 82% of hydroponics, representatively, and iron (Fe) was not detected. The fresh weight of lettuce harvested from the FHP15 treatment was 38 g, only a 30% of marketable lettuce yield. The T-N and P contents of the leaf tissue grown under the FHP15 treatment were close to the optimal level; however, the K, Ca, and iron (Fe) contents were less than the optimal with no deficiency symptom.

• Journal of Service Research and Studies
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• v.7 no.4
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• pp.51-68
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• 2017

Financial technology (FinTech) is an emerging financial service sector include innovations in financial literacy and investment, retail banking, education, and crypto-currencies like bitcoin. One of the crucial branch of financial technology-third-party payment (TPP) is undergoing rapid growth, with online/mobile systems replacing offline financial systems. System quality and user attitudes are key perceptions driving third-party payment usage, the importance of these perceptions, however, may be different with countries as users' thinking varies from country to country. Thus, the purpose of this study is to elaborate how factors differ from China to Korea by drawing on the unified theory of acceptance and use of technology (UTAUT2). Additionally, this study also aims to propose a multi-attribute evaluation of the third-party online payment system based on analytic hierarchy process (AHP), fuzzy sets and technique for order performance by similarity to ideal solution (TOPSIS), to examine the relative importance of the perceptions influencing new technology adoption intention. The results showed that the price value has the most significant influence on Chinese perceptions, while the perceived credibility has the most significant effect on Korean perceptions. Sub-criteria also performs different results to Chinese and Korean third-party online payment system.