• Animal Bioscience
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• v.36 no.12
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• pp.1796-1805
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• 2023

Objective: This study aims to identify heat shock protein70-2 (HSP70-2) and protamine-1 (PRM1) mRNA and protein in Madura bull sperm and demonstrate their relation as bull fertility biomarkers. Methods: The Madura bull fertility rates were grouped based on the percentage of first service conception rate (%FSCR) as high fertility (HF) (79.04%; n = 4), and low fertility (LF) (65.84%; n = 4). mRNA of HSP70-2 and PRM1 with peptidylprolyl isomerase A (PPIA) as a housekeeping gene were determined by quantitative real-time polymerase chain reaction, while enzyme-linked immunoassay was used to measure protein abundance. In the post-thawed semen samples, sperm motility, viability, acrosome integrity, and sperm DNA fragmentation index were analyzed. Data analysis was performed on the measured parameters of semen quality, relative mRNA expression, and protein abundance of HSP70-2 and PRM1, among the bulls with various fertility levels (HF and LF) in a one-way analysis of variance analysis. The Pearson correlation was used to analyze the relationship between semen quality, mRNA, proteins, and fertility rate. Results: Relative mRNA expression and protein abundance of HSP70-2 and PRM1 were detected and were found to be highly expressed in bulls with HF (p<0.05) and were associated with several parameters of semen quality. Conclusion: HSP70-2 and PRM1 mRNA and protein molecules have great potential to serve as molecular markers for determining bull fertility.

• Journal of Microbiology and Biotechnology
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• v.24 no.10
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• pp.1308-1318
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• 2014

The specific freshwater environment of reservoirs formed by streams has not been well studied. In this paper, the bacterioplankton community in such a reservoir, the Huangqian Reservoir in eastern China, was described using culture-independent molecular methods. We found that the most dominant bacterioplankton were affiliated with Cyanobacteria, followed by Betaproteobacteria, Bacteroidetes, Gammaproteobacteria, and Actinobacteria. Both bacterial abundance and diversity increased along the direction of water flow, and the 16S rRNA gene copy number in the water outlet was nearly an order of magnitude higher than that in the water inlet. Pearson correlation analyses indicated that nitrate had a significantly negative correlation with the bacterial abundance (p < 0.05) and that ammonium was positively correlated with bacterial abundance (p < 0.05). Interestingly, owing to a remarkably negative correlation (p < 0.01), the ratio of nitrate and ammonium might serve as a good pre dictor of the relative abundance of bacterioplankton. According to redundancy analysis, nitrate and dissolved oxygen were the major factors influencing the bacterial communities. In addition, we attempted to determine the reasons why such a reservoir could maintain good ecological balance for a period of decades, and we found that the environmental factors and bacterial communities both played critical roles. This research will benefit our understanding of bacterial communities and their surrounding environments in freshwater ecosystems.

• Mass Spectrometry Letters
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• v.2 no.1
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• pp.1-7
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• 2011

China's rapid economic growth has resulted in significant environmental side effects. Therefore, China has been interested in reducing her dependence on foreign oil and gas by developing technologies needed for hydrogen, in addition to her increasing energy mix of nuclear and renewable energy form, such as solar and wind power. There are three isotopes of hydrogen, i.e. protium (P or H), deuterium (D), and tritium (T). Both deuterium and tritium are important materials in nuclear fuel cycle industry. Tritium is one of the critical radioactive nuclides. Planning for and implementing contamination control as a part of normal operation and maintenance activities is an important function in any hydrogen facility, especially tritium facility. The development of hydrogen isotopes analysis is the key issues in this area. Mass spectrometry (MS) with medium (about 600) and high resolution (> 1,400) is commercially available; however, the routine analysis of hydrogen isotopes is done with low-resolution MS (< 200) in China. This paper summarizes the progress of MS measurement technology for hydrogen isotope abundance in China, focusing on our lab's research program and technical status. An analyzing method has been introduced for accurate measurement of tritium abundance in the H.D.T system by low resolution MAT-253 MS. The quotient of compression ratio coefficient is determined by building up equipment for laboratory-scale preparation of secondary standard gases and by considering the difference in sensitivity between hydrogen isotopes. The results show that the measured value is reproducible within the relative error range of 0.8% for gas samples of different tritium abundance.

• Korean Journal of Ecology and Environment
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• v.46 no.4
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• pp.541-550
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• 2013

The objective of this study was to understand the distribution characteristics of largemouth bass as an exotic species in relation to water chemistry. The survey was conducted in 10 reservoirs in Korea that showed different properties in size, location and eutrophic state. Total number of fish species observed in the artificial reservoirs was 52 (13 family) and the relative abundance of the bass was 13% of the total, which is the third dominant species in the whole sample. The relative abundance of bass was the highest in the Pyungtak reservoir (60%), whereas the Daechung reservoir showed the lowest abundance (only 3%). Although no significance difference statistically were founded in the relationship between bass abundance and water quality parameters. The reservoir trophic state showed some relationships. As result, the higher abundance was observed in hypertrophic reservoirs that located in the esturine regions compared to other large and medium reservoirs classified as meso- or eutrphic state. In conclusion, bass distribution in the reservoir ecosystem can not be directly explained by water chemistry only but other environmental factors should be considered.

• ALGAE
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• v.20 no.4
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• pp.345-352
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• 2005

In order to investigate the distribution of phytoplankton community in the coastal waters of the Chuksan Harbor, East Sea, the abundance and biomass of phytoplankton have been evaluated through seasonal interval sampling from April 2000 to October 2002. A total of 363 different phytoplankton species was observed and most of them were composed of diatoms. The mean abundance and chlorophyll-a concentration of phytoplankton during the study period ranged from 56 ${\times}$ $10^3$ to 720 ${\times}$ $10^3$ cells $L^{-1}$ and from 0.78 to 3.29 μg chl-a $L^{-1}$, respectively. The relative contribution of the size-fractionated phytoplankton to phytoplankton community showed difference according to seasons. The average contribution of nano-phytoplankton(<20 $\mu$m) was over 50% in the total abundance and biomass of the phytoplankton. Our results show that nano-phytoplankton play an important role in the southern coastal waters of the East Sea. And the environmental factors such as suspended substances, phosphates and silicates were positively correlated with the abundances and biomass of phytoplankton.

• ALGAE
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• v.31 no.1
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• pp.41-48
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• 2016

The clonal seaweed Chondrus crispus (Rhodophyta, Gigartinales) forms extensive stands at low intertidal elevations on wave-sheltered rocky shores of the North Atlantic. This study investigates if this bushy alga acts as a foundation species in such habitats. The abundance (percent cover) of C. crispus, all other algae, and invertebrates was measured in 390 quadrats spanning 350 km of coast in Nova Scotia, Canada. In these low-intertidal habitats, fucoid algae are the largest organisms and can form extensive canopies, but their cover was unrelated to benthic species richness and to C. crispus cover. Species richness, however, increased with C. crispus cover from low to intermediate cover values, showing little change towards full C. crispus cover. Species composition (a combined measure of species identity and their relative abundance) differed between quadrats with low (0-1%) and high (60-100%) cover of C. crispus. High C. crispus cover was associated to more invertebrate species but fewer algal species than low C. crispus cover. However, the average abundance of algal and invertebrate species occurring in both cover groups was often higher under high C. crispus cover, contributing to a higher average richness at the quadrat scale. Overall, only 16% of the observed variation in species richness was explained by C. crispus cover. Therefore, this study suggests that C. crispus acts as a foundation species but with a moderate influence.

• The Korean Journal of Ecology
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• v.11 no.1
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• pp.1-15
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• 1988

Studies on species compostion, promary production of benthic algal assemblage were carried out along a channel of the salt marsh near Inch'on, Kyonggi Bay, Korea. Possible biological, physical and KDICical factors controlling the aglal assemblage were also examined. The oveall diatoms were encountered one hundred and thirty-seven taxa, of which the dominant species were Paralia sulcata and Cymatosira belgica. These two species accounted for 32.6% relative abundance throughout the study period. Diatom taxa had no clear seasonal pattern in abundance analysis. But at the algal blooming period in spring, euglenoids occurred with a high abundance. The chlorophyll a content of benthic algae showed definite seasonal pattern. The algal biomass of the appeared to influence the spatial fluctuation in the algal biomass of the channel was regulated primarily by water content of sediment. Grazing by zoobenthos apperaed to influence the spatial fluctuation in the algal biomass of the sediment surface. The algal photosynthesis was measured in the laboratory with oxygen method. Photo-synthetic rate was independent of the temperature under the lower irradiance. The gross production from March to November was estimated to be 190g C/m2 at the channel slope. Photosynthetic efficiency was 0.37% on the basis of the photosyntherically active radiation for the study period.

• Journal of Astronomy and Space Sciences
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• v.28 no.1
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• pp.13-16
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• 2011

The $CH_3OH$ $4_2-5_1$ E transition was observed toward the Sgr B2 region, including the Principal Cloud and its surroundings. This methanol transition shows an extended emission along the 2'N cloud, which is believed to be colliding with the Principal Cloud and may trigger the massive star formation in this cloud. This extended methanol emission may also suggest that the 2'N cloud is under shocks. We derive total methanol column density $N(CH_3OH)\;=\;2.9{\pm}0.3{\times}10^{14}\;cm^{-2}$ toward the peak position of the extended emission. The fractional abundance of methanol is about 10.9, relative to the estimated total $H_2$ abundance, which is similar to the methanol abundances in quiet gas phase.

• Genomics & Informatics
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• v.16 no.4
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• pp.21.1-21.7
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• 2018

Metritis, the inflammation of the uterus caused by polymicrobial infections, is a prevalent and costly disease to the dairy industry as it decreases milk yield, survival, and the welfare of dairy cows. Although affected cows are treated with broad-spectrum antibiotics such as ceftiofur, endometrial and ovarian function are not fully recovered, which results in subfertility and infertility. According to culture-dependent studies, uterine pathogens include Escherichia coli, Trueperella pyogenes, Fusobacterium necrophorum, and Prevotella melaninogenica. Recent studies using high-throughput sequencing observed very low relative abundance of Escherichia coli, Trueperella pyogenes, and Prevotella melaninogenica in cows with metritis. Herein, we propose that metritis is associated with a dysbiosis of the uterine microbiota, which is characterized by high abundance of Bacteroides, Porphyromonas, and Fusobacterium.

• Journal of Astronomy and Space Sciences
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• v.35 no.1
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• pp.1-6
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• 2018

The reanalysis of the previously published abundance pattern of mild barium star HD202109 (${\zeta}$ Cyg) and the chemical compositions of 129 thin disk barium stars facilitated the search for possible correlations of different stellar parameters with second ionization potentials of chemical elements. Results show that three valuable correlations exist in the atmospheres of barium stars. The first is the relationship between relative abundances and second ionization potentials. The second is the age dependence of mean correlation coefficients of relative abundances vs. second ionization potentials, and the third one is the changes in correlation coefficients of relative abundances vs. second ionization potentials as a function of stellar spatial velocities and overabundances of s-process elements. These findings demonstrate the possibility of hydrogen and helium accretion from the interstellar medium on the atmospheres of barium stars.