• Restorative Dentistry and Endodontics
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• 제25권2호
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• pp.170-179
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• 2000

The rh-BMP-4 is a subgroup of TGF-${\beta}$ superfamily. The application of rh-BMP in alveolar bony defect was reported to new alveolar bone and new cementum formation. For minimized complications following tooth replantation, a operator must replant a tooth fast at the pertinent position. This study was to evaluate the effect of rh-BMP-4 on periodontal regeneration and root resorption following tooth replantation in rats. The 50 Sprague-Dawley rats weighting about 130gm were used in this study. The animals were divided into three groups. Group 1 ; immediate replantation after extraction : Group 2 ; replantation stored teeth extraction of first molar, the removal of periodontal ligament with collagenase, and etching with citric acid : Group 3 ; replantation stored teeth with treated rh-BMP-4 in mesial root. Experimental animals were sacrificed 3, 7, 14 days after replantation by heart infusion. The maxillae were removed, fixed, demineralized, dehydrated, infiltrated and embedded with JB-4 mixture. For light microscopic observation, 5 micron sections were cut and stained with toluidine blue. The results of this study were as follows : 1. After experimental 3 days, all groups were observed dead space between periodontum and root. 2. After experimental 7 days, group 1 and group 3 were observed filling periodontal fibers between alveolar bone and root but group 2 were not. 3. After experimental 7 days, group 3 were observed appearance of attached cementoblast like cell on root surface. Group 1 were observed regular arrangement of fibroblasts and collagen fibers at ${\times}400$ observation. 4. After experimental 14 days, all group were observed filling periodontal fibers between alveolar bone and root. Group 1 were observed normal arrangement of periodontal fibers. Group 3 were observed less abnormal arrangement of periodontal fibers. Group 2 were not observed functional normal arrangement of periodontal fibers. 5. After experimental 14 days, group 2 and 3 were observed several root resorption and irregular root surface but group 1 were not. These results suggest that the rh-BMP-4 can stimulate cementogenesis and enhance to attach collagen fibers.

• 한국재난정보학회 논문집
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• 제17권4호
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• pp.684-693
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• 2021

연구목적: 아두이노를 이용하여 실시간 대지저항 데이터를 취득할 수 있는 스마트 대지저항 측정장치를 개발하여 낙뢰 등 이상전압으로부터 안전한 설비환경을 구축하는것에 목적이 있다. 연구방법: 본 논문은 아두이노와 전력선 통신(PLC) 체계를 갖춘 대지저항 취득 및 분석 시스템을 개발하여 설계모델과 적용사례를 연구하였다. 경남지역의 풍력발전 단지 내 일부 부지를 테스트 베드로 선정하여 신기술을 적용한 실시간 대지저항 데이터를 취득하였다. 전극배열은 웨너(Wenner) 4전극배열과 슐렘버거(Schlumberger) 전극배열을 혼용한 스마트 전극배열을 채택하였다. 연구결과: 본 기술의 특징은 첫 번째로 스마트 다 전극의 깊이를 각기 다르게 편성해 층간에 특이성을 가지는 지층 구조에도 취득 데이터의 오차범위를 축소하였다. 두 번째로 스마트 접지전극에서 취득한 대지저항 데이터의 정보를 사물인터넷으로 실시간 송·수신이 가능하도록 IT융합 기술을 적용하였다. 마지막으로 규칙적인 관리 체계를 구축하고 Server에 축적된 빅 데이터를 분석하여 다양한 요소들의 변화추이를 확인할 수 있으며, IT융합 환경에 최적의 접지 알고리즘과 접지시스템 설계 모델링이 가능하다. 결론: 본 기술은 4차 산업 시대에 근간이 되는 도시 기반시설에 낙뢰로 인한 서지(Surge)의 피해를 줄이고 최적화된 접지시스템 모델을 설계하여 사용자의 안전과 생명을 보호 할 것이다. 또한 순수 국산 기술력의 지적재산권을 확보하여 포스트 코로나 시대에 팬데믹으로 정체되어있는 우리산업의 일자리 창출과 활기를 불어넣는 효과도 기대된다.

• 대한시과학회지
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• 제20권4호
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• pp.561-568
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• 2018

목적 : 각막실질 내 다발 구조로 이루어진 콜라겐섬유의 크기와 규칙적인 배열은 투명성과 매우 밀접한 상관성을 가지고 있다. 시뮬레이션을 이용하여 배열구조 및 콜라겐섬유층 두께에 따른 광투과율의 변화를 확인하고자 하였다. 방법 : 시뮬레이션 소프트웨어인 OptiFDTD로 각막실질 내 콜라겐섬유를 정육각형, 육각형, 사각형 및 자유형으로 각각 배열하였고 이에 따른 광투과율을 분석하였다. 사각형 배열에 대하여 시뮬레이션 공간상에 있는 콜라겐섬유의 개수가 동일할 때 밀도변화에 따른 광투과율을 확인하고 콜라겐섬유의 개수와 밀도가 변화할 때 광투과율을 조사하였다. 결과 : 콜라겐섬유의 개수가 동일할 때 사각형, 정육각형, 자유형 및 육각형의 배열구조 순서로 밀도가 작아지고, 섬유층의 두께가 두꺼워진다. 배열구조를 변화시켜 광투과율을 측정한 결과 동일한 위치의 검출기에서 측정된 광투과율은 배열구조에 관계없이 거의 유사하였다. 검출기 D0, D1, D2 및 D3에서 각각 사각형, 육각형과 사각형, 정육각형 및 정육각형 배열구조에서 최대투과율로 나타났으며, 육각형, 자유형, 육각형과 사각형 및 사각형 배열구조에서 최소 투과율로 나타났다. 하지만, 최대 투과율과 최소 투과율의 차이는 1% 이내로 거의 유사하였다. 콜라겐섬유의 개수가 동일할 때 사각형 배열구조에서 밀도변화에 따른 광투과율은 섬유층 두께가 증가할수록 광투과율은 감소하였다. 또한, 두께가 증가하면서 콜라겐섬유의 개수가 감소하였을 때 광투과율이 더 많이 감소하였다. 결론 : 콜라겐 배열구조가 변화하여도 광투과율은 배열구조와 관계없이 거의 유사하게 나타났다. 하지만, 배열구조의 변화에 따라 콜라겐섬유층의 두께가 변화하였고, 두께가 증가할수록 광투과율이 감소하였다. 즉, 광투과율은 배열구조보다는 콜라겐섬유층의 두께와 더 밀접한 관계를 가지고 있음을 확인하였다.

• 한국의류산업학회지
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• 제6권1호
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• pp.17-22
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• 2004

The purpose of this study was to obtain the basic element of patterns, which is the motif, from the existing art works and use it to create and simulate a variety of patterns to show the practical use of the computer. Natural dyed works of geometric shapes like squares and 1/4 of circles, each dyed with a different natural dyes, were produced and motifs were extracted. Then using the 4D box in Adobe Photoshop v.6.0, the developing pattern change and simulation effects when using different patterns and different repetition settings were examined. Observations were made as the motifs were replicated. Different patterns like a diagonal line, a square and a circle appeared. In order to find out the effects according to the changes, the motifs from work's square portions, circle centre, and the composition of eight motifs were extracted and used. The repeated patterns according to the extracted motifs simply showed that in the case of just repeating and arranging, square patterns appeared. By replicating and arranging, the motif and the allophone were matched and patterns with regular lines were formed, like a twill, pointed twill line. By setting the direction to Y and X in the repetition methodes, a typical half-drop arrangement or a brick pattern arrangement were formed according to 1/4, 2/4, 3/4, 1/3, or 2/3 in changes. Also the steepness of the slope changes quickly or in turn slowly as the rhombus shape appears. However in the case of a composed motif, an ogee pattern appeared. Lastly, by 3-D mapping patterns like a slant line, pointed twill patterns, diamond patterns, and the repeat patterns of a motif with a circle and a line combined, and a circle motif, an optical illusion could be observed.

• 대한치과교정학회지
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• 제50권6호
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• pp.391-400
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• 2020

Objective: Increased gingival elasticity has been implicated as the cause of relapse following orthodontic rotational tooth movement and approaches to reduce relapse are limited. This study aimed to investigate the effects of sulforaphane (SFN), an inhibitor of osteoclastogenesis, on gene expression in gingival fibroblasts and relapse after rotational tooth movement in beagle dogs. Methods: The lower lateral incisors of five beagle dogs were rotated. SFN or dimethylsulfoxide (DMSO) were injected into the supra-alveolar gingiva of the experimental and control group, respectively, and the effect of SFN on relapse tendency was evaluated. Changes in mRNA expression of extracellular matrix components associated with gingival elasticity in beagles were investigated by real-time polymerase chain reaction. Morphology and arrangement of collagen fibers were observed on Masson's trichrome staining of buccal gingival tissues of experimental and control teeth. Results: SFN reduced the amount and percentage of relapse of orthodontic rotation. It also decreased the gene expression of lysyl oxidase and increased the gene expression of matrix metalloproteinase (MMP) 1 and MMP 12, compared with DMSO control subjects. Histologically, collagen fiber bundles were arranged irregularly and were not well connected in the SFN-treated group, whereas the fibers extended in parallel and perpendicular directions toward the gingiva and alveolar bone in a more regular and well-ordered arrangement in the DMSO-treated group. Conclusions: Our findings demonstrated that SFN treatment may be a promising pharmacologic approach to prevent orthodontic rotational relapse caused by increased gingival elasticity of rotated teeth in beagle dogs.

• 대한세포병리학회지
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• 제9권1호
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• pp.69-78
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• 1998

There is a lot of difficulty in the diagnosis of follicular lesions of the thyroid by fine needle aspiration cytology(FNAC). The main purpose of this report is offering more guidance regarding the cytologic appearance to distinguish follicular neoplasm from nodular golfer and laying stress on the presence of mixed group. The histologic and cytologic findings of 23 follicular neoplasms and 13 nodular (adenomatous) getters were reviewed. Histologic specimens were classified into the microfollicular(MIF), mixed(MIX), and nodular getter(NG) groups. The comparison of histologic patterns with histologic diagnosis revealed that all the lesions with predominantly microfollicular, trabecular, or solid pattern were follicular carcinoma and all the lesions with predominantly macrofollicular pattern were nodular goiter. The distinguishing cytologic features for the MIF group were irregular cell arrangement in cell groups(100%, p=0.00001), absence of atrophic follicular cells(100%, p=0.0007), abundant microfollicles(100%, 0=0.002), pleomorphic nuclei(100%, p=0.002), not predominant syncytial smear pattern(100%, p=0.002), heterochromatin(100%, p=0.032), absence of macrofollicles(100%, p=0.038), scant colloid(100%, 0=0.04), clear back-ground(83%, p=0.00006), and uniform sized follicles(83%, p=0.014). And regular cell arrangement(honeycomb appearance) in cell groups(85%, p=0.0000), atrophic change of follicular cells(69%, p=0.0002), syncytial smear pattern(54%, p=0.000), monomorphic nuclei(85%, p=0.008), and hemorrhagic background(100%, p=0.027) were characteristic features of the NG group. Seventeen out of 36 cases(47%) were the MIX group composed of combined cytologic features of the MIF and NG groups. Therefore the frequent presence of the MIX group is considered to be main cause of the difficulty in the diagnosis of follicular lesions by FNAC. The mixed morphologic feature may support the hypothesis of a biologic 'continuum' between nodular goiter and follicular neoplasm of thyroid gland.

• 대한한방내과학회지
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• 제32권2호
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• pp.259-277
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• 2011

Objectives : The purpose of this study was to investigate two subjects: the diagnostic value of bilateral lowering of electrical activity at point H4,5,6 of Ryodoraku and the mechanism for Ryodoraku phenomena. Methods : Electrical activities of Ryodoraku test and electrogastrography recorded simultaneously and monitored continuously from 16 cases of functional dyspeptic patients were collected and their variations were grouped by the topics of discussion which were peculiarity, stability, lagging, alterability, and anomaly. Ryodoraku recordings obtained from 6 patients with different gastrointestinal diseases and 1 normal healthy person were used as control. The results are discussed with Nakatani's suggestion, theory of sympathetic nerve and Meridian Principle, respectively. Finely, coincidence of stomach arrangement between anatomy and meridian system in Ryodoraku was also evaluated. Results : Time-course variation showed a regular relationship between the typical pattern of Ryodoraku at point H4,5,6 and gastric myoelectrical activity. However, an irregular relationship and atypical pattern of Ryodoraku occasionally appeared. A literature search suggested that electrical response at the Ryodoraku point H4,5,6 may be dependent on an afferent sympathetic spinal reflex transmitted from the stomach. However, there was no evidence for making clear whether bilateral lowering of electrical activity at this point was induced by hypofunction of local sympathetic nerve in the skin itself or of signals transmitted from the gastric sympathetic nerve or not. The coincidence of 19% could not provide a visceral arrangement of the stomach between anatomy and meridian systems. Conclusions : Bilateral lowering of electrical activity at Ryodoraku point H4,5,6 has value as a diagnostic index for gastric dysmotility of functional dyspepsia. This phenomenon is associated with spinal reflex transmitted from the afferent sympathetic nerve in the stomach but not that of meridian function.

• Environmental Analysis Health and Toxicology
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• 제24권2호
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• pp.159-167
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• 2009

To investigate the alleviative effects of Peonia japonica water extract(PJWE) on inflammation and skin barrier damage, both the irradiation of UVB and the application of squalene monohydroperoxide (Sq-OOH) to the backs of hairless mice were performed for 4 weeks. And at the same time experimental materials were applied topically. The skin erythema indices for the positive control (PC, 0.01% retinoic acid) and experimental (E, PJWE) groups were lower than that of the control (C) group. Whereas both the lipid and water capacities for the PC and E groups were higher than those of the C group. Epidermis and dermis of the C group were remarkably thickened in comparison with the PC and E groups. Relatively much less number of inflammatory cells, including lymphocytes, neutrophils and macrophages were found in dermis of the PC and E groups compared with the C group. Lipid lamellae of the C group were broken severely showing an irregular arrangement and lipid content was much reduced. Whereas those of the PC and E groups were almost intact with a regular arrangement, which were similar to that of the N group. Taken the results all together, it was confirmed that PJWE could be effective natural herbal material for the alleviation of inflammation and skin barrier damage in hairless mice skin which were induced by UVB and Sq-OOH.

• 한국방재학회 논문집
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• 제8권3호
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• pp.149-155
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• 2008

사석의 안정성 분석을 위해 Isbash식, California 도로국식, Netherlands간이식, ASCE식, Pilarczk식, Maynord식의 검토를 수리모형실험을 통해서 수행하였다. 사석의 경우는 직경과 중량이 커질수록 한계유속이 크게 나왔으며, 그에 따른 수심에 대한 사석의 직경비, Froude수, 평균유속에 따른 전단유속비, Shields수는 상관성이 크게 나왔다. 공칭직경에 대한 안정식은 6가지 식이 모두 크게 산정하고 있고, 그중 미 공병단에서 제시한 Maynord식이 비교적 잘 맞는 것으로 나타났다. 중량산정식의 경우 Isbash식, Netherlands식, Pilarczk식은 중량이 유속에 대하여 과대추정 되었고, 켈리포니아주 정부 도로국 식이 실험결과와 일치하는 것으로 나타났다. 단위중량이 작은 석고재료에 의한 모형사석 실험에서는 형상계수가 클수록 한계유속이 크게 나타났고, 또한 임의배열일 때보다 규칙배열일 경우 한계유속이 크게 측정 되었다. 이로서 형상계수와 결속의 정도가 사석의 안정성에 중요한 함수라는 것을 알 수 있다.

• 대한치과교정학회지
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• 제23권1호
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• pp.89-100
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• 1993

The purpose of this study was to evaluate the effect of orthodontic force on periodontal cellular activity by immunoperoxidase stain of epidermal growth factor, one of the tissue hormone. And supplementarily, to investigate of the changes of periodontal structures, periodontium was stained by H-E, Masson's Trichrome, P. A. S. stain after orthodontic force application. The experimental animals were four young adult dogs of average 8 month old. The fixed orthodontic appliance was cemented on mandibular right 4th premolar and 1st molar of each animal as experimental site. Mandibular left 4th premolar area of the same animal was used as control. The appliance consist of two silver crown soldered with 0.030' tube, $0.018\times0.022'$ S.S. sectional arch wire, and 0.009' open coil spring for manifestating of orthodontic force for bodily tooth movement of mandibular 4th premolar toward mesial direction. Experimental group was sacrificed at 1, 2, 3, 5 weeks from beginning of the experiment, and was investigated immunohistochemically and bistochemically by several staining methods. Findings were as follows: 1. The degree of EGF staining in control group was highest in epithelium of periodontium, and osteoclasts, osteoblasts and fibroblasts around the capillary were stained at higher level in periodontium. Generally, control group shows positive distribution of EGF all around the periodontal area. 2. The degree of EGF staining in control and 5 week group were similar, and did not show the significant different level between tension and pressure side. 3. All of 1, 2, 3 week group showed the same staining degree and distribution of EGF, and the tension side was more positive reaction of EGF stain than the pressure side. 4. The features of collagen fiber and periodontal fiber arrangement observed by H-E, Masson's Trichrome and P. A. S. stain revealed that oblique periodontal fibers were strectched in tension side, compressed in pressure side of all experimental group. Some fiber group in pressure side of 5 week group recovered the regular arrangement along the capillaries.